################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 800 CGCG CG^CG AflIII 617 ACGTGT A^C[GA][CT]GT AspHI 706 GTGCTC G[AT]GC[AT]^C AvaI 640 CCCGGG C^[CT]CG[GA]G BamHI 269 GGATCC G^GATCC BbsI 597 GAAGAC GAAGAC(2/6) BmyI 706 GTGCTC G[AGT]GC[ACT]^C BpuAI 597 GAAGAC GAAGAC(2/6) BsgI 646 GTGCAG GTGCAG(16/14) BsiHKAI 706 GTGCTC G[AT]GC[AT]^C BsmBI 396 CGTCTC CGTCTC(1/5) BsmFI 843 GGGAC GGGAC(10/14) BsmI 826 GCATTC GAATGC(1/-1) BsoBI 640 CCCGGG C^[CT]CG[GA]G Bsp1286I 706 GTGCTC G[AGT]GC[ACT]^C BsrBI 740 GAGCGG CCGCTC(-3/-3) BstAPI 862 GCATGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 672 GGTCACC G^GT[ACGT]ACC BstPI 672 GGTCACC G^GT[ACGT]ACC BstUI 800 CGCG CG^CG BstXI 339 CCAGGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 694 CTCAG C^T[ACGT]AG DraII 111 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 588 CCACGG C^C[GA][CT]GG EcoO109I 111 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 672 GGTCACC G^GT[ACGT]ACC HaeII 1045 AGCGCC [GA]GCGC^[CT] HgaI 798 GACGC GACGC(5/10) HincII 1021 GTTGAC GT[CT]^[GA]AC HindII 1021 GTTGAC GT[CT]^[GA]AC MaeII 617 ACGT A^CGT MvnI 800 CGCG CG^CG NaeI 918 GCCGGC GCC^GGC NgoMIV 918 GCCGGC G^CCGGC NspI 862 GCATGC [GA]CATG^[CT] PflMI 167 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 763 GAGTC GAGTC(4/5) SexAI 338 ACCAGGT A^CC[AT]GGT SmaI 640 CCCGGG CCC^GGG SphI 862 GCATGC GCATG^C TfiI 769 GAATC G^A[AT]TC Tsp509I 130 AATT ^AATT Van91I 167 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 734 CCATCCGAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 640 CCCGGG C^CCGGG XmaI 640 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaII 819 GGTCC G^G[AT]CC 844 GGACC BcnI 640 CCCGG CC^[GC]GG 641 CCGGG BglI 496 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 913 GCCCTGCCGGC BpmI 1041 CTCCAG CTGGAG(16/14) 382 CTGGAG BspMI 676 ACCTGC ACCTGC(4/8) 575 GCAGGT BsrFI 918 GCCGGC [GA]^CCGG[CT] 977 ACCGGC BstBI 118 TTCGAA TT^CGAA 986 TTCGAA BstYI 269 GGATCC [GA]^GATC[CT] 530 GGATCT Cfr10I 918 GCCGGC [GA]^CCGG[CT] 977 ACCGGC FauI 258 CCCGC CCCGC(4/6) 555 CCCGC HinfI 763 GAGTC G^A[ACGT]TC 769 GAATC MaeIII 673 GTCAC ^GT[ACGT]AC 796 GTGAC MflI 269 GGATCC [GA]^GATC[CT] 530 GGATCT MslI 699 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 804 CATGGTGGTG MspA1I 259 CCGCTG C[AC]G^C[GT]G 624 CAGCGG NciI 640 CCCGG CC^[GC]GG 641 CCGGG NspV 118 TTCGAA TT^CGAA 986 TTCGAA SfuI 118 TTCGAA TT^CGAA 986 TTCGAA TaqI 119 TCGA T^CGA 987 TCGA Tsp45I 673 GTCAC ^GT[GC]AC 796 GTGAC TspRI 10 CAGTG CA[GC]TG[ACGT][ACGT]^ 168 CAGTG TthHB8I 119 TCGA T^CGA 987 TCGA XhoII 269 GGATCC [GA]^GATC[CT] 530 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BalI BbvI BsaI BsaJI BsiYI BslI BsmAI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MboII MluNI MnlI MscI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI