(Linear) (Six-base) MAPSORT of: KIEE1120.seq Check: 3440 from: 1 to: 1092 KIEE1120.orf With 182 enzymes: SgfI * July 3, 2012 16:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 685 1092 Size: 685 407 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 616 1092 Size: 616 476 AflIII A'CryG_T Cuts at: 0 617 1092 Size: 617 475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 721 1092 Size: 721 371 AlwNI CAG_nnn'CTG Cuts at: 0 173 292 581 1092 Size: 173 119 289 511 Fragments arranged by size: 511 289 173 119 AvaI C'yCGr_G Cuts at: 0 640 1092 Size: 640 452 BamHI G'GATC_C Cuts at: 0 269 1092 Size: 269 823 BbsI GAAGACnn'nnnn_ Cuts at: 0 604 1092 Size: 604 488 BglI GCCn_nnn'nGGC Cuts at: 0 502 919 1092 Size: 502 417 173 Fragments arranged by size: 502 417 173 BmgBI CAC'GTC Cuts at: 0 618 1092 Size: 618 474 BmrI ACTGGGnnnn_n' Cuts at: 0 63 1092 Size: 63 1029 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 1026 1092 Size: 403 623 66 Fragments arranged by size: 623 403 66 BsaI GGTCTCn'nnnn_ Cuts at: 0 298 424 431 1068 1092 Size: 298 126 7 637 24 Fragments arranged by size: 637 298 126 24 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 85 115 382 412 1092 Size: 85 30 267 30 680 Fragments arranged by size: 680 267 85 30 30 BseYI C'CCAG_C Cuts at: 0 5 360 839 1092 Size: 5 355 479 253 Fragments arranged by size: 479 355 253 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 667 1092 Size: 667 425 BsiHKAI G_wGCw'C Cuts at: 0 710 1092 Size: 710 382 BsmI GAATG_Cn' Cuts at: 0 826 1092 Size: 826 266 BsmBI CGTCTCn'nnnn_ Cuts at: 0 402 1092 Size: 402 690 Bsp1286I G_dGCh'C Cuts at: 0 710 1092 Size: 710 382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 566 685 1092 Size: 566 119 407 Fragments arranged by size: 566 407 119 BsrBI CCG'CTC Cuts at: 0 742 1092 Size: 742 350 BsrDI GCAATG_nn' Cuts at: 0 191 486 515 1092 Size: 191 295 29 577 Fragments arranged by size: 577 295 191 29 BsrFI r'CCGG_y Cuts at: 0 918 977 1092 Size: 918 59 115 Fragments arranged by size: 918 115 59 BstAPI GCAn_nnn'nTGC Cuts at: 0 868 1092 Size: 868 224 BstBI TT'CG_AA Cuts at: 0 119 987 1092 Size: 119 868 105 Fragments arranged by size: 868 119 105 BstEII G'GTnAC_C Cuts at: 0 672 1092 Size: 672 420 BstXI CCAn_nnnn'nTGG Cuts at: 0 346 1092 Size: 346 746 BstYI r'GATC_y Cuts at: 0 269 530 1092 Size: 269 261 562 Fragments arranged by size: 562 269 261 BtgI C'CryG_G Cuts at: 0 588 1092 Size: 588 504 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 123 297 1092 Size: 123 174 795 Fragments arranged by size: 795 174 123 BtsI GCAGTG_nn' Cuts at: 0 16 1092 Size: 16 1076 EaeI y'GGCC_r Cuts at: 0 227 431 503 731 1092 Size: 227 204 72 228 361 Fragments arranged by size: 361 228 227 204 72 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 616 1026 1092 Size: 403 213 410 66 Fragments arranged by size: 410 403 213 66 EcoO109I rG'GnC_Cy Cuts at: 0 112 1092 Size: 112 980 HaeII r_GCGC'y Cuts at: 0 1049 1092 Size: 1049 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 402 434 754 786 1015 1047 1051 1083 Size: 402 32 320 32 229 32 4 32 Cuts at: 1083 1092 Size: 9 Fragments arranged by size: 402 320 229 32 32 32 32 9 4 HincII GTy'rAC Cuts at: 0 1023 1092 Size: 1023 69 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 550 1092 Size: 550 542 MscI TGG'CCA Cuts at: 0 433 505 733 1092 Size: 433 72 228 359 Fragments arranged by size: 433 359 228 72 MslI CAynn'nnrTG Cuts at: 0 703 808 1092 Size: 703 105 284 Fragments arranged by size: 703 284 105 MspA1I CmG'CkG Cuts at: 0 261 626 1092 Size: 261 365 466 Fragments arranged by size: 466 365 261 NaeI GCC'GGC Cuts at: 0 920 1092 Size: 920 172 NgoMIV G'CCGG_C Cuts at: 0 918 1092 Size: 918 174 NspI r_CATG'y Cuts at: 0 866 1092 Size: 866 226 PflMI CCAn_nnn'nTGG Cuts at: 0 173 1092 Size: 173 919 PfoI T'CCnGG_A Cuts at: 0 380 526 1092 Size: 380 146 566 Fragments arranged by size: 566 380 146 SexAI A'CCwGG_T Cuts at: 0 338 1092 Size: 338 754 SmaI CCC'GGG Cuts at: 0 642 1092 Size: 642 450 SphI G_CATG'C Cuts at: 0 866 1092 Size: 866 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 82 141 790 1092 Size: 82 59 649 302 Fragments arranged by size: 649 302 82 59 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 741 1092 Size: 741 351 XmaI C'CCGG_G Cuts at: 0 640 1092 Size: 640 452 Enzymes that do cut: AarI AcuI AflIII AlfI AlwNI AvaI BamHI BbsI BglI BmgBI BmrI BpmI BsaI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI Eco57MI EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NgoMIV NspI PflMI PfoI SexAI SmaI SphI TaqII XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BspEI BspHI BsrGI BssHII BssSI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI