Restriction map for
pFN21AE9558
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbrPI BcgI BciVI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
MamI MfeI MluI MroI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 248 GGTACC G^GTACC
Asp718I 248 GGTACC G^GTACC
AspHI 1111 GAGCAC G[AT]GC[AT]^C
BamHI 914 GGATCC G^GATCC
BbeI 1253 GGCGCC GGCGC^C
BbsI 931 GTCTTC GAAGAC(2/6)
BclI 947 TGATCA T^GATCA
BfaI 1409 CTAG C^TAG
BpuAI 931 GTCTTC GAAGAC(2/6)
BsaI 1384 GAGACC GGTCTC(1/5)
BsiHKAI 1111 GAGCAC G[AT]GC[AT]^C
BsmAI 1384 GAGAC GTCTC(1/5)
BssSI 985 CTCGTG CACGAG(-5/-1)
BstAPI 747 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 118 GGTGACC G^GT[ACGT]ACC
BstPI 118 GGTGACC G^GT[ACGT]ACC
BstYI 914 GGATCC [GA]^GATC[CT]
DrdI 625 GACCTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1206 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 118 GGTGACC G^GT[ACGT]ACC
FbaI 947 TGATCA T^GATCA
HaeII 1253 GGCGCC [GA]GCGC^[CT]
KasI 1253 GGCGCC G^GCGCC
KpnI 248 GGTACC GGTAC^C
KspI 680 CCGCGG CCGC^GG
MaeI 1409 CTAG C^TAG
MaeII 872 ACGT A^CGT
MflI 914 GGATCC [GA]^GATC[CT]
NarI 1253 GGCGCC GG^CGCC
PflMI 1080 CCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 121 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1342 CTGCAG CTGCA^G
PvuII 979 CAGCTG CAG^CTG
SacII 680 CCGCGG CCGC^GG
SapI 1143 GCTCTTC GCTCTTC(1/4)
SfoI 1253 GGCGCC GGC^GCC
SmaI 329 CCCGGG CCC^GGG
Tsp509I 615 AATT ^AATT
Van91I 1080 CCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 563 CCAGCTCCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 914 GGATCC [GA]^GATC[CT]
XmaCI 329 CCCGGG C^CCGGG
XmaI 329 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 531 CGCG CG^CG
681 CGCG
AcyI 1075 GGCGTC G[GA]^CG[CT]C
1253 GGCGCC
AlwI 915 GATCC GGATC(4/5)
914 GGATC
AlwNI 636 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1174 CAGGGCCTG
ApaI 301 GGGCCC GGGCC^C
461 GGGCCC
AvaI 329 CCCGGG C^[CT]CG[GA]G
775 CCCGAG
BbvCI 495 CCTCAGC CCTCAGC(-5/-2)
1161 CCTCAGC
BsaHI 1075 GGCGTC G[GA]^CG[CT]C
1253 GGCGCC
BsoBI 329 CCCGGG C^[CT]CG[GA]G
775 CCCGAG
Bsp1407I 410 TGTACA T^GTACA
431 TGTACA
BsrDI 518 GCAATG GCAATG(2/0)
890 GCAATG
BsrGI 410 TGTACA T^GTACA
431 TGTACA
BstUI 531 CGCG CG^CG
681 CGCG
CfoI 1131 GCGC GCG^C
1254 GCGC
DpnII 915 GATC ^GATC
948 GATC
DraIII 265 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1081 CACGAAGTG
EarI 1144 CTCTTC CTCTTC(1/4)
1156 CTCTTC
HgaI 544 GCGTC GACGC(5/10)
1076 GCGTC
HhaI 1131 GCGC GCG^C
1254 GCGC
Hin1I 1075 GGCGTC G[GA]^CG[CT]C
1253 GGCGCC
HinP1I 1131 GCGC G^CGC
1254 GCGC
HinfI 534 GAATC G^A[ACGT]TC
1372 GATTC
Ksp632I 1144 CTCTTC CTCTTC(1/4)
1156 CTCTTC
MboI 915 GATC ^GATC
948 GATC
MvnI 531 CGCG CG^CG
681 CGCG
NdeII 915 GATC ^GATC
948 GATC
PpuMI 65 AGGTCCC [GA]G^G[AT]CC[CT]
595 AGGACCC
Sau3AI 915 GATC ^GATC
948 GATC
SspBI 410 TGTACA T^GTACA
431 TGTACA
TfiI 534 GAATC G^A[AT]TC
1372 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AvaII BalI
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeIII MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I