(Circular) (Six-base) MAPSORT of: pFN21AE9558.seq Check: 5088 from: 1 to: 6136 pFN21AE9558 6136 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 2253 3457 2253 Size: 1204 4932 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2779 4199 5583 5851 1632 Size: 1147 1420 1384 268 1917 Fragments arranged by size: 1917 1420 1384 1147 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6136 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3362 1184 Size: 2178 3958 AlwNI CAG_nnn'CTG Cuts at: 2646 3184 3879 5451 2646 Size: 538 695 1572 3331 Fragments arranged by size: 3331 1572 695 538 ApaI G_GGCC'C Cuts at: 2310 2470 2310 Size: 160 5976 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 3445 3671 3823 5932 1493 Size: 1952 226 152 2109 1697 Fragments arranged by size: 2109 1952 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2334 2780 3461 1870 Size: 73 391 446 681 4545 Fragments arranged by size: 4545 681 446 391 73 BamHI G'GATC_C Cuts at: 2919 3466 3833 2919 Size: 547 367 5222 Fragments arranged by size: 5222 547 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2253 2265 2822 3128 3249 Size: 325 217 627 466 12 557 306 121 Cuts at: 3249 3258 3457 4912 618 Size: 9 199 1455 1842 Fragments arranged by size: 1842 1455 627 557 466 325 306 217 199 121 12 9 BanII G_rGCy'C Cuts at: 1391 2310 2470 3431 3455 1391 Size: 919 160 961 24 4072 Fragments arranged by size: 4072 961 919 160 24 BbeI G_GCGC'C Cuts at: 3262 3262 Size: 6136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2929 928 Size: 971 1030 4135 Fragments arranged by size: 4135 1030 971 BbvCI CC'TCA_GC Cuts at: 2501 3167 2501 Size: 666 5470 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4327 4361 1903 Size: 34 50 34 2306 34 3678 Fragments arranged by size: 3678 2306 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 2952 1548 Size: 1404 4732 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4747 136 Size: 107 122 71 631 605 3075 1525 Fragments arranged by size: 3075 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6131 6131 Size: 6136 BlpI GC'TnA_GC Cuts at: 3553 3553 Size: 6136 Bme1580I G_kGCm'C Cuts at: 1790 2310 2470 2827 3010 3131 4197 5353 1790 Size: 520 160 357 183 121 1066 1156 2573 Fragments arranged by size: 2573 1156 1066 520 357 183 160 121 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2356 3867 4825 Size: 416 53 83 399 24 939 1511 958 Cuts at: 4825 442 Size: 1753 Fragments arranged by size: 1753 1511 958 939 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4796 1239 Size: 3557 2579 Bpu10I CC'TnA_GC Cuts at: 2082 2144 2501 2597 3167 2082 Size: 62 357 96 570 5051 Fragments arranged by size: 5051 570 357 96 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4261 5126 5424 4261 Size: 865 298 4973 Fragments arranged by size: 4973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3383 4799 882 Size: 617 1884 1416 2219 Fragments arranged by size: 2219 1884 1416 617 BsaAI yAC'GTr Cuts at: 493 1204 3927 493 Size: 711 2723 2702 Fragments arranged by size: 2723 2702 711 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3081 3259 3943 4325 Size: 53 83 186 936 1548 178 684 382 Cuts at: 4325 4960 275 Size: 635 1451 Fragments arranged by size: 1548 1451 936 684 635 382 186 178 83 53 BsaWI w'CCGG_w Cuts at: 4569 4967 5241 5388 5799 4569 Size: 398 274 147 411 4906 Fragments arranged by size: 4906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2159 2189 2729 2759 3212 Size: 30 165 30 756 30 540 30 453 Cuts at: 3212 3242 3349 3379 1178 Size: 30 107 30 3935 Fragments arranged by size: 3935 756 540 453 165 107 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 2465 2800 3296 1186 Size: 1210 69 335 496 4026 Fragments arranged by size: 4026 1210 496 335 69 BseYI C'CCAG_C Cuts at: 2761 2817 3076 3335 5339 5835 2761 Size: 56 259 259 2004 496 3062 Fragments arranged by size: 3062 2004 496 259 259 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2495 2500 2735 3245 1163 Size: 573 759 5 235 510 4054 Fragments arranged by size: 4054 759 573 510 235 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4347 5375 1540 Size: 275 186 1585 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3120 4197 4282 5353 3120 Size: 1077 85 1071 3903 Fragments arranged by size: 3903 1077 1071 85 BsmI GAATG_Cn' Cuts at: 3647 3740 3647 Size: 93 6043 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2470 2827 3010 3120 3131 3431 Size: 399 520 160 357 183 110 11 300 Cuts at: 3431 3455 4197 4282 5353 1391 Size: 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 399 357 300 183 160 110 85 24 11 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2265 2757 2766 3491 844 Size: 1421 492 9 725 3489 Fragments arranged by size: 3489 1421 725 492 9 BsrBI CCG'CTC Cuts at: 1394 1823 2081 2663 3286 4023 1394 Size: 429 258 582 623 737 3507 Fragments arranged by size: 3507 737 623 582 429 258 BsrDI GCAATG_nn' Cuts at: 65 1248 2530 2902 4631 4805 65 Size: 1183 1282 372 1729 174 1396 Fragments arranged by size: 1729 1396 1282 1183 372 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4780 4967 1700 Size: 110 2970 187 2869 Fragments arranged by size: 2970 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2415 2436 96 Size: 2319 21 3796 Fragments arranged by size: 3796 2319 21 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 2990 4196 5208 2990 Size: 1206 1012 3918 Fragments arranged by size: 3918 1206 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2758 794 Size: 1964 4172 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 2123 1184 Size: 939 5197 BstXI CCAn_nnnn'nTGG Cuts at: 2170 2375 2621 2170 Size: 205 246 5685 Fragments arranged by size: 5685 246 205 BstYI r'GATC_y Cuts at: 1972 2919 3466 3833 4218 4235 5687 5773 5785 Size: 947 547 367 385 17 1452 86 12 Cuts at: 5785 6131 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 947 547 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2163 2229 2685 3325 513 Size: 552 1098 66 456 640 3324 Fragments arranged by size: 3324 1098 640 552 456 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 2512 2859 3652 4446 4466 2512 Size: 347 793 794 20 4182 Fragments arranged by size: 4182 794 793 347 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3440 3440 Size: 6136 DraIII CAC_nnn'GTG Cuts at: 2275 3091 2275 Size: 816 5320 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2636 5143 809 Size: 507 1320 2507 1802 Fragments arranged by size: 2507 1802 1320 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2207 2232 2406 Size: 54 1425 50 275 18 377 25 174 Cuts at: 2406 2484 2682 2712 2769 2943 2994 3045 3322 Size: 78 198 30 57 174 51 51 277 Cuts at: 3322 3583 4472 6101 8 Size: 261 889 1629 43 Fragments arranged by size: 1629 1425 889 377 277 275 261 198 174 174 78 57 54 51 51 50 43 30 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3155 3167 4066 1353 Size: 1802 12 899 3423 Fragments arranged by size: 3423 1802 899 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3130 4723 5097 5243 387 Size: 1064 138 1541 1593 374 146 1280 Fragments arranged by size: 1593 1541 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2779 4199 4796 5583 5851 1239 Size: 393 1147 1420 597 787 268 1524 Fragments arranged by size: 1524 1420 1147 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3215 3215 Size: 6136 EcoO109I rG'GnC_Cy Cuts at: 2071 2466 2467 2497 2601 2740 3181 3306 2071 Size: 395 1 30 104 139 441 125 4901 Fragments arranged by size: 4901 441 395 139 125 104 30 1 EcoRI G'AATT_C Cuts at: 3445 3823 3445 Size: 378 5758 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3169 3169 Size: 6136 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 3262 5283 1678 Size: 1584 2021 2531 Fragments arranged by size: 2531 2021 1584 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3212 3244 4777 4809 4851 Size: 32 135 32 1640 32 1533 32 42 Cuts at: 4851 4883 1373 Size: 32 2626 Fragments arranged by size: 2626 1640 1533 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3726 669 Size: 988 281 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6136 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KasI G'GCGC_C Cuts at: 3258 3258 Size: 6136 KpnI G_GTAC'C Cuts at: 2257 3461 2257 Size: 1204 4932 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3210 5250 5434 1305 Size: 1905 2040 184 2007 Fragments arranged by size: 2040 2007 1905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 2486 2714 2771 2945 Size: 54 1425 343 576 78 228 57 174 Cuts at: 2945 2996 3047 10 Size: 51 51 3099 Fragments arranged by size: 3099 1425 576 343 228 174 78 57 54 51 51 MslI CAynn'nnrTG Cuts at: 518 1166 4094 4453 4612 518 Size: 648 2928 359 159 2042 Fragments arranged by size: 2928 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2687 2986 3255 3552 4229 5377 5622 1606 Size: 1081 299 269 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 1081 677 299 297 269 245 NarI GG'CG_CC Cuts at: 3259 3259 Size: 6136 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5584 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 3494 5039 1132 Size: 97 2265 1545 2229 Fragments arranged by size: 2265 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2461 2847 3124 1348 Size: 1113 386 277 4360 Fragments arranged by size: 4360 1113 386 277 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 3091 1108 Size: 1983 4153 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 2601 2071 Size: 530 5606 PshAI GACnn'nnGTC Cuts at: 1613 2130 1613 Size: 517 5619 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PspOMI G'GGCC_C Cuts at: 2306 2466 2306 Size: 160 5976 PstI C_TGCA'G Cuts at: 830 3351 3488 830 Size: 2521 137 3478 Fragments arranged by size: 3478 2521 137 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 2986 2986 Size: 6136 SacII CC_GC'GG Cuts at: 2688 2688 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SapI GCTCTTCn'nnn_ Cuts at: 3155 3155 Size: 6136 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SfcI C'TryA_G Cuts at: 826 1045 2449 2608 3347 3484 4619 5300 5491 Size: 219 1404 159 739 137 1135 681 191 Cuts at: 5491 826 Size: 1471 Fragments arranged by size: 1471 1404 1135 739 681 219 191 159 137 SfoI GGC'GCC Cuts at: 3260 3260 Size: 6136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 2336 3463 1872 Size: 464 1127 4545 Fragments arranged by size: 4545 1127 464 SmlI C'TyrA_G Cuts at: 820 1017 1943 4240 5141 5403 820 Size: 197 926 2297 901 262 1553 Fragments arranged by size: 2297 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6136 StyI C'CwwG_G Cuts at: 513 1065 2261 2345 2481 3396 3575 513 Size: 552 1196 84 136 915 179 3074 Fragments arranged by size: 3074 1196 915 552 179 136 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2311 4163 4180 4333 4518 4963 325 Size: 1255 731 1852 17 153 185 445 1498 Fragments arranged by size: 1852 1498 1255 731 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2415 2436 4382 Size: 274 80 33 51 1443 438 21 1946 Cuts at: 4382 6094 96 Size: 1712 138 Fragments arranged by size: 1946 1712 1443 438 274 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 3349 3381 6096 6128 2159 Size: 32 1158 32 2715 32 2167 Fragments arranged by size: 2715 2167 1158 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2575 2575 Size: 6136 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 2334 3461 1870 Size: 464 1127 4545 Fragments arranged by size: 4545 1127 464 XmnI GAAnn'nnTTC Cuts at: 4265 4265 Size: 6136 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI