(Linear) (Six-base) MAPSORT of: KIEE9558.seq Check: 6407 from: 1 to: 1431 KIEE9558.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. Acc65I G'GTAC_C Cuts at: 0 248 1431 Size: 248 1183 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 774 1431 Size: 774 657 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1357 1431 Size: 1357 74 AlwNI CAG_nnn'CTG Cuts at: 0 641 1179 1431 Size: 641 538 252 Fragments arranged by size: 641 538 252 ApaI G_GGCC'C Cuts at: 0 305 465 1431 Size: 305 160 966 Fragments arranged by size: 966 305 160 AvaI C'yCGr_G Cuts at: 0 329 775 1431 Size: 329 446 656 Fragments arranged by size: 656 446 329 BamHI G'GATC_C Cuts at: 0 914 1431 Size: 914 517 BanI G'GyrC_C Cuts at: 0 248 260 817 1123 1244 1253 1431 Size: 248 12 557 306 121 9 178 Fragments arranged by size: 557 306 248 178 121 12 9 BanII G_rGCy'C Cuts at: 0 305 465 1426 1431 Size: 305 160 961 5 Fragments arranged by size: 961 305 160 5 BbeI G_GCGC'C Cuts at: 0 1257 1431 Size: 1257 174 BbsI GAAGACnn'nnnn_ Cuts at: 0 924 1431 Size: 924 507 BbvCI CC'TCA_GC Cuts at: 0 496 1162 1431 Size: 496 666 269 Fragments arranged by size: 666 496 269 BclI T'GATC_A Cuts at: 0 947 1431 Size: 947 484 Bme1580I G_kGCm'C Cuts at: 0 305 465 822 1005 1126 1431 Size: 305 160 357 183 121 305 Fragments arranged by size: 357 305 305 183 160 121 BmrI ACTGGGnnnn_n' Cuts at: 0 351 1431 Size: 351 1080 Bpu10I CC'TnA_GC Cuts at: 0 77 139 496 592 1162 1431 Size: 77 62 357 96 570 269 Fragments arranged by size: 570 357 269 96 77 62 BsaI GGTCTCn'nnnn_ Cuts at: 0 1378 1431 Size: 1378 53 BsaHI Gr'CG_yC Cuts at: 0 1076 1254 1431 Size: 1076 178 177 Fragments arranged by size: 1076 178 177 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 154 184 724 754 1207 1237 1344 1374 Size: 154 30 540 30 453 30 107 30 Cuts at: 1374 1431 Size: 57 Fragments arranged by size: 540 453 154 107 57 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 391 460 795 1291 1431 Size: 391 69 335 496 140 Fragments arranged by size: 496 391 335 140 69 BseYI C'CCAG_C Cuts at: 0 756 812 1071 1330 1431 Size: 756 56 259 259 101 Fragments arranged by size: 756 259 259 101 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 495 730 1240 1431 Size: 490 5 235 510 191 Fragments arranged by size: 510 490 235 191 5 BsiHKAI G_wGCw'C Cuts at: 0 1115 1431 Size: 1115 316 Bsp1286I G_dGCh'C Cuts at: 0 305 465 822 1005 1115 1126 1426 1431 Size: 305 160 357 183 110 11 300 5 Fragments arranged by size: 357 305 300 183 160 110 11 5 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 260 752 761 1431 Size: 260 492 9 670 Fragments arranged by size: 670 492 260 9 BsrBI CCG'CTC Cuts at: 0 76 658 1281 1431 Size: 76 582 623 150 Fragments arranged by size: 623 582 150 76 BsrDI GCAATG_nn' Cuts at: 0 525 897 1431 Size: 525 372 534 Fragments arranged by size: 534 525 372 BsrGI T'GTAC_A Cuts at: 0 410 431 1431 Size: 410 21 1000 Fragments arranged by size: 1000 410 21 BssSI C'ACGA_G Cuts at: 0 985 1431 Size: 985 446 BstAPI GCAn_nnn'nTGC Cuts at: 0 753 1431 Size: 753 678 BstEII G'GTnAC_C Cuts at: 0 118 1431 Size: 118 1313 BstXI CCAn_nnnn'nTGG Cuts at: 0 165 370 616 1431 Size: 165 205 246 815 Fragments arranged by size: 815 246 205 165 BstYI r'GATC_y Cuts at: 0 914 1431 Size: 914 517 BtgI C'CryG_G Cuts at: 0 158 224 680 1320 1431 Size: 158 66 456 640 111 Fragments arranged by size: 640 456 158 111 66 BtsI GCAGTG_nn' Cuts at: 0 507 854 1431 Size: 507 347 577 Fragments arranged by size: 577 507 347 DraIII CAC_nnn'GTG Cuts at: 0 270 1086 1431 Size: 270 816 345 Fragments arranged by size: 816 345 270 DrdI GACnn_nn'nnGTC Cuts at: 0 631 1431 Size: 631 800 EaeI y'GGCC_r Cuts at: 0 202 227 401 479 677 707 764 938 Size: 202 25 174 78 198 30 57 174 Cuts at: 938 989 1040 1317 1431 Size: 51 51 277 114 Fragments arranged by size: 277 202 198 174 174 114 78 57 51 51 30 25 EarI CTCTTCn'nnn_ Cuts at: 0 1150 1162 1431 Size: 1150 12 269 Fragments arranged by size: 1150 269 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1125 1431 Size: 1125 306 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 774 1431 Size: 774 657 EcoNI CCTnn'n_nnAGG Cuts at: 0 1210 1431 Size: 1210 221 EcoO109I rG'GnC_Cy Cuts at: 0 66 461 462 492 596 735 1176 1301 Size: 66 395 1 30 104 139 441 125 Cuts at: 1301 1431 Size: 130 Fragments arranged by size: 441 395 139 130 125 104 66 30 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1164 1431 Size: 1164 267 HaeII r_GCGC'y Cuts at: 0 1257 1431 Size: 1257 174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1207 1239 1431 Size: 1207 32 192 Fragments arranged by size: 1207 192 32 KasI G'GCGC_C Cuts at: 0 1253 1431 Size: 1253 178 KpnI G_GTAC'C Cuts at: 0 252 1431 Size: 252 1179 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1205 1431 Size: 1205 226 MscI TGG'CCA Cuts at: 0 403 481 709 766 940 991 1042 1431 Size: 403 78 228 57 174 51 51 389 Fragments arranged by size: 403 389 228 174 78 57 51 51 MspA1I CmG'CkG Cuts at: 0 682 981 1250 1431 Size: 682 299 269 181 Fragments arranged by size: 682 299 269 181 NarI GG'CG_CC Cuts at: 0 1254 1431 Size: 1254 177 PasI CC'CwG_GG Cuts at: 0 456 842 1119 1431 Size: 456 386 277 312 Fragments arranged by size: 456 386 312 277 PflMI CCAn_nnn'nTGG Cuts at: 0 1086 1431 Size: 1086 345 PpuMI rG'GwC_Cy Cuts at: 0 66 596 1431 Size: 66 530 835 Fragments arranged by size: 835 530 66 PshAI GACnn'nnGTC Cuts at: 0 125 1431 Size: 125 1306 PspOMI G'GGCC_C Cuts at: 0 301 461 1431 Size: 301 160 970 Fragments arranged by size: 970 301 160 PstI C_TGCA'G Cuts at: 0 1346 1431 Size: 1346 85 PvuII CAG'CTG Cuts at: 0 981 1431 Size: 981 450 SacII CC_GC'GG Cuts at: 0 683 1431 Size: 683 748 SapI GCTCTTCn'nnn_ Cuts at: 0 1150 1431 Size: 1150 281 SfcI C'TryA_G Cuts at: 0 444 603 1342 1431 Size: 444 159 739 89 Fragments arranged by size: 739 444 159 89 SfoI GGC'GCC Cuts at: 0 1255 1431 Size: 1255 176 SmaI CCC'GGG Cuts at: 0 331 1431 Size: 331 1100 StyI C'CwwG_G Cuts at: 0 256 340 476 1391 1431 Size: 256 84 136 915 40 Fragments arranged by size: 915 256 136 84 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 306 1431 Size: 306 1125 TatI w'GTAC_w Cuts at: 0 410 431 1431 Size: 410 21 1000 Fragments arranged by size: 1000 410 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 154 186 1344 1376 1431 Size: 154 32 1158 32 55 Fragments arranged by size: 1158 154 55 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 570 1431 Size: 570 861 XmaI C'CCGG_G Cuts at: 0 329 1431 Size: 329 1102 Enzymes that do cut: Acc65I AcuI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI Bme1580I BmrI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI MmeI MscI MspA1I NarI PasI PflMI PpuMI PshAI PspOMI PstI PvuII SacII SapI SfcI SfoI SmaI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI