(Circular) (Six-base) MAPSORT of: pFN21AE9439.seq Check: 3374 from: 1 to: 6352 pFN21AE9439 6352 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2105 2350 2105 Size: 245 6107 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3513 3695 1937 Size: 1576 182 4594 Fragments arranged by size: 4594 1576 182 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2300 3164 4415 5799 6067 1632 Size: 668 864 1251 1384 268 1917 Fragments arranged by size: 1917 1384 1251 864 668 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6155 AflIII A'CryG_T Cuts at: 1203 2115 5251 6228 1203 Size: 912 3136 977 1327 Fragments arranged by size: 3136 1327 977 912 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 2061 5081 2061 Size: 3020 3332 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6352 AlwNI CAG_nnn'CTG Cuts at: 4095 5667 4095 Size: 1572 4780 ApaI G_GGCC'C Cuts at: 2032 2501 3025 2032 Size: 469 524 5359 Fragments arranged by size: 5359 524 469 ApaLI G'TGCA_C Cuts at: 4409 5565 4409 Size: 1156 5196 ApoI r'AATT_y Cuts at: 1493 3661 3887 4039 6148 1493 Size: 2168 226 152 2109 1697 Fragments arranged by size: 2168 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 2892 3024 3451 3677 1870 Size: 73 949 132 427 226 4545 Fragments arranged by size: 4545 949 427 226 132 73 BamHI G'GATC_C Cuts at: 3682 4049 3682 Size: 367 5985 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3673 5128 618 Size: 325 217 627 1886 1455 1842 Fragments arranged by size: 1886 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2032 2343 2501 3025 3671 1391 Size: 641 311 158 524 646 4072 Fragments arranged by size: 4072 646 641 524 311 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2325 2599 2682 3028 3106 3541 928 Size: 971 426 274 83 346 78 435 3739 Fragments arranged by size: 3739 971 435 426 346 274 83 78 BbvCI CC'TCA_GC Cuts at: 2300 2300 Size: 6352 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4543 4577 1903 Size: 34 2606 34 3678 Fragments arranged by size: 3678 2606 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4244 5454 4244 Size: 1210 5142 BclI T'GATC_A Cuts at: 1548 1548 Size: 6352 BfrBI ATG'CAT Cuts at: 2572 2572 Size: 6352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3020 3029 3159 Size: 107 122 71 631 605 1348 9 130 Cuts at: 3159 4963 136 Size: 1804 1525 Fragments arranged by size: 1804 1525 1348 631 605 130 122 107 71 9 BglII A'GATC_T Cuts at: 3372 6347 3372 Size: 2975 3377 BlpI GC'TnA_GC Cuts at: 2589 3149 3769 2589 Size: 560 620 5172 Fragments arranged by size: 5172 620 560 Bme1580I G_kGCm'C Cuts at: 1790 2032 2501 3025 3135 4413 5569 1790 Size: 242 469 524 110 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 524 469 242 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3204 4083 5041 Size: 416 53 83 399 24 1787 879 958 Cuts at: 5041 442 Size: 1753 Fragments arranged by size: 1787 1753 958 879 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2719 2719 Size: 6352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2357 2618 2700 3275 5012 1239 Size: 1118 261 82 575 1737 2579 Fragments arranged by size: 2579 1737 1118 575 261 82 Bpu10I CC'TnA_GC Cuts at: 2300 2300 Size: 6352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3630 4477 5342 5640 3630 Size: 847 865 298 4342 Fragments arranged by size: 4342 865 847 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2767 2842 5015 882 Size: 617 1268 75 2173 2219 Fragments arranged by size: 2219 2173 1268 617 75 BsaAI yAC'GTr Cuts at: 493 1204 2932 4143 493 Size: 711 1728 1211 2702 Fragments arranged by size: 2702 1728 1211 711 BsaBI GATnn'nnATC Cuts at: 3185 6346 3185 Size: 3161 3191 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 4159 4541 5176 Size: 53 83 186 936 479 2147 382 635 Cuts at: 5176 275 Size: 1451 Fragments arranged by size: 2147 1451 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3420 4785 5183 5457 5604 6015 3420 Size: 1365 398 274 147 411 3757 Fragments arranged by size: 3757 1365 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6127 Fragments arranged by size: 6127 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 3595 1186 Size: 847 1562 3943 Fragments arranged by size: 3943 1562 847 BseYI C'CCAG_C Cuts at: 3018 3162 3442 5555 6051 3018 Size: 144 280 2113 496 3319 Fragments arranged by size: 3319 2113 496 280 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2185 1163 Size: 573 449 5330 Fragments arranged by size: 5330 573 449 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2624 3802 4563 5591 1540 Size: 275 186 623 1178 761 1028 2301 Fragments arranged by size: 2301 1178 1028 761 623 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 3596 4413 4498 5569 3435 Size: 161 817 85 1071 4218 Fragments arranged by size: 4218 1071 817 161 85 BsmI GAATG_Cn' Cuts at: 3863 3956 3863 Size: 93 6259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2343 2501 3025 3135 3435 3596 Size: 399 242 311 158 524 110 300 161 Cuts at: 3596 3671 4413 4498 5569 1391 Size: 75 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 524 399 311 300 242 161 158 110 85 75 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 4241 5971 4241 Size: 1730 4622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2105 2350 3105 3707 844 Size: 1261 245 755 602 3489 Fragments arranged by size: 3489 1261 755 602 245 BsrBI CCG'CTC Cuts at: 1394 1823 3409 4239 1394 Size: 429 1586 830 3507 Fragments arranged by size: 3507 1586 830 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2197 4847 5021 65 Size: 1183 949 2650 174 1396 Fragments arranged by size: 2650 1396 1183 949 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4996 5183 1700 Size: 110 3186 187 2869 Fragments arranged by size: 3186 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6352 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 4412 5424 4412 Size: 1012 5340 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6352 BstBI TT'CG_AA Cuts at: 5156 5156 Size: 6352 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstXI CCAn_nnnn'nTGG Cuts at: 2023 2023 Size: 6352 BstYI r'GATC_y Cuts at: 1972 2925 3372 3682 4049 4434 4451 5903 5989 Size: 953 447 310 367 385 17 1452 86 Cuts at: 5989 6001 6347 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 953 447 385 367 346 310 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2059 2721 2059 Size: 662 5690 BtgI C'CryG_G Cuts at: 513 1065 2004 2706 2793 3310 513 Size: 552 939 702 87 517 3555 Fragments arranged by size: 3555 939 702 552 517 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5613 Fragments arranged by size: 5613 683 56 BtsI GCAGTG_nn' Cuts at: 2310 3868 4662 4682 2310 Size: 1558 794 20 3980 Fragments arranged by size: 3980 1558 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6317 DraI TTT'AAA Cuts at: 3656 3656 Size: 6352 DraIII CAC_nnn'GTG Cuts at: 2163 2163 Size: 6352 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2454 3480 3799 Size: 54 1425 50 275 18 624 1026 319 Cuts at: 3799 4688 6317 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1026 889 624 319 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2495 3613 4282 1353 Size: 1142 1118 669 3423 Fragments arranged by size: 3423 1142 1118 669 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2357 2618 2700 3164 3275 4415 Size: 393 668 57 261 82 464 111 1140 Cuts at: 4415 5012 5799 6067 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1140 787 668 597 464 393 268 261 111 82 57 EcoNI CCTnn'n_nnAGG Cuts at: 2881 2881 Size: 6352 EcoO109I rG'GnC_Cy Cuts at: 2020 2028 2029 2497 2498 3166 2020 Size: 8 1 468 1 668 5206 Fragments arranged by size: 5206 668 468 8 1 1 EcoRI G'AATT_C Cuts at: 3661 4039 3661 Size: 378 5974 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 5499 1678 Size: 3821 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 4993 5025 5067 Size: 32 135 32 475 32 2914 32 42 Cuts at: 5067 5099 1373 Size: 32 2626 Fragments arranged by size: 2914 2626 475 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3696 3942 669 Size: 988 281 1758 246 3079 Fragments arranged by size: 3079 1758 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6352 HpaI GTT'AAC Cuts at: 3942 3942 Size: 6352 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5466 5650 1305 Size: 4161 184 2007 Fragments arranged by size: 4161 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3482 10 Size: 54 1425 343 1650 2880 Fragments arranged by size: 2880 1650 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3068 4310 4669 4828 518 Size: 648 1902 1242 359 159 2042 Fragments arranged by size: 2042 1902 1242 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2526 3398 3768 4445 5593 5838 1606 Size: 920 872 370 677 1148 245 2120 Fragments arranged by size: 2120 1148 920 872 677 370 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4861 Fragments arranged by size: 4861 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6352 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NsiI A_TGCA'T Cuts at: 2574 2574 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 2119 2576 3710 5255 1132 Size: 97 890 457 1134 1545 2229 Fragments arranged by size: 2229 1545 1134 890 457 97 PasI CC'CwG_GG Cuts at: 1348 2016 2938 1348 Size: 668 922 4762 Fragments arranged by size: 4762 922 668 PciI A'CATG_T Cuts at: 2115 5251 2115 Size: 3136 3216 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6352 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PmlI CAC'GTG Cuts at: 2932 2932 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3233 3265 4421 4453 5934 5966 3233 Size: 32 1156 32 1481 32 3619 Fragments arranged by size: 3619 1481 1156 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2475 1613 Size: 862 5490 PsiI TTA'TAA Cuts at: 3922 3922 Size: 6352 PspOMI G'GGCC_C Cuts at: 2028 2497 3021 2028 Size: 469 524 5359 Fragments arranged by size: 5359 524 469 PstI C_TGCA'G Cuts at: 830 2317 3704 830 Size: 1487 1387 3478 Fragments arranged by size: 3478 1487 1387 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SfcI C'TryA_G Cuts at: 826 1045 2313 3393 3700 4835 5516 5707 826 Size: 219 1268 1080 307 1135 681 191 1471 Fragments arranged by size: 1471 1268 1135 1080 681 307 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 2894 3026 3679 1872 Size: 1022 132 653 4545 Fragments arranged by size: 4545 1022 653 132 SmlI C'TyrA_G Cuts at: 820 1017 1943 3451 3645 4456 5357 5619 820 Size: 197 926 1508 194 811 901 262 1553 Fragments arranged by size: 1553 1508 926 901 811 262 197 194 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3710 1132 Size: 2578 3774 SspI AAT'ATT Cuts at: 5 52 4276 5 Size: 47 4224 2081 Fragments arranged by size: 4224 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6352 StyI C'CwwG_G Cuts at: 513 1065 2004 2781 3791 513 Size: 552 939 777 1010 3074 Fragments arranged by size: 3074 1010 939 777 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2930 3452 4379 4396 4549 4734 5179 Size: 1255 1350 522 927 17 153 185 445 Cuts at: 5179 325 Size: 1498 Fragments arranged by size: 1498 1350 1255 927 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4598 6310 96 Size: 274 80 33 51 1443 2621 1712 138 Fragments arranged by size: 2621 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6312 6344 6312 Size: 32 6320 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2828 2848 2828 Size: 20 6332 XhoI C'TCGA_G Cuts at: 1943 3451 1943 Size: 1508 4844 XmaI C'CCGG_G Cuts at: 1870 2892 3024 3677 1870 Size: 1022 132 653 4545 Fragments arranged by size: 4545 1022 653 132 XmnI GAAnn'nnTTC Cuts at: 3374 4481 3374 Size: 1107 5245 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI