(Circular) (Six-base) MAPSORT of: pFN21AE9425.seq Check: 5183 from: 1 to: 8371 pFN21AE9425 8371 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6181 278 Size: 53 83 186 936 4645 2468 Fragments arranged by size: 4645 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3006 5714 1937 Size: 1069 2708 4594 Fragments arranged by size: 4594 2708 1069 Acc65I G'GTAC_C Cuts at: 5692 5692 Size: 8371 AclI AA'CG_TT Cuts at: 1563 6498 6871 1563 Size: 4935 373 3063 Fragments arranged by size: 4935 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3894 4286 5635 6434 7818 8086 1632 Size: 2262 392 1349 799 1384 268 1917 Fragments arranged by size: 2262 1917 1384 1349 799 392 268 AflII C'TTAA_G Cuts at: 820 1017 2461 3835 820 Size: 197 1444 1374 5356 Fragments arranged by size: 5356 1444 1374 197 AflIII A'CryG_T Cuts at: 1203 3931 4374 4782 7270 8247 1203 Size: 2728 443 408 2488 977 1327 Fragments arranged by size: 2728 2488 1327 977 443 408 AgeI A'CCGG_T Cuts at: 2228 7202 2228 Size: 4974 3397 AhdI GACnn_n'nnGTC Cuts at: 3135 7100 3135 Size: 3965 4406 AleI CACnn'nnGTG Cuts at: 1166 3291 1166 Size: 2125 6246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4658 1184 Size: 3474 4897 AlwNI CAG_nnn'CTG Cuts at: 4264 6114 7686 4264 Size: 1850 1572 4949 Fragments arranged by size: 4949 1850 1572 ApaI G_GGCC'C Cuts at: 3361 3361 Size: 8371 ApaLI G'TGCA_C Cuts at: 6428 7584 6428 Size: 1156 7215 ApoI r'AATT_y Cuts at: 1493 2330 2803 2944 3697 4003 4114 4838 4952 Size: 837 473 141 753 306 111 724 114 Cuts at: 4952 5680 5906 6058 8167 1493 Size: 728 226 152 2109 1697 Fragments arranged by size: 2109 1697 837 753 728 724 473 306 226 152 141 114 111 AseI AT'TA_AT Cuts at: 160 1032 6925 160 Size: 872 5893 1606 Fragments arranged by size: 5893 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8371 AvaI C'yCGr_G Cuts at: 1870 1943 5696 1870 Size: 73 3753 4545 Fragments arranged by size: 4545 3753 73 BamHI G'GATC_C Cuts at: 4072 4270 5701 6068 4072 Size: 198 1431 367 6375 Fragments arranged by size: 6375 1431 367 198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5692 7147 618 Size: 325 217 627 3905 1455 1842 Fragments arranged by size: 3905 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2224 2544 2792 2888 3361 5690 1391 Size: 833 320 248 96 473 2329 4072 Fragments arranged by size: 4072 2329 833 473 320 248 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2595 2739 4010 5397 928 Size: 971 696 144 1271 1387 3902 Fragments arranged by size: 3902 1387 1271 971 696 144 BbvCI CC'TCA_GC Cuts at: 2978 3727 2978 Size: 749 7622 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2365 2399 6562 6596 1903 Size: 34 428 34 4163 34 3678 Fragments arranged by size: 4163 3678 428 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6263 7473 6263 Size: 1210 7161 BclI T'GATC_A Cuts at: 1548 1548 Size: 8371 BfrBI ATG'CAT Cuts at: 2386 2624 2711 2386 Size: 238 87 8046 Fragments arranged by size: 8046 238 87 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6982 136 Size: 107 122 71 631 605 5310 1525 Fragments arranged by size: 5310 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5058 8366 5058 Size: 3308 5063 BlpI GC'TnA_GC Cuts at: 5788 5788 Size: 8371 Bme1580I G_kGCm'C Cuts at: 1790 3361 6432 7588 1790 Size: 1571 3071 1156 2573 Fragments arranged by size: 3071 2573 1571 1156 BmgBI CAC'GTC Cuts at: 1318 4377 1318 Size: 3059 5312 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2049 3494 3643 Size: 416 53 83 399 24 632 1445 149 Cuts at: 3643 6102 7060 442 Size: 2459 958 1753 Fragments arranged by size: 2459 1753 1445 958 632 416 399 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3318 7031 1239 Size: 2079 3713 2579 Fragments arranged by size: 3713 2579 2079 Bpu10I CC'TnA_GC Cuts at: 2978 3065 3727 4631 2978 Size: 87 662 904 6718 Fragments arranged by size: 6718 904 662 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2353 3350 3475 3881 4130 6496 7361 7659 2353 Size: 997 125 406 249 2366 865 298 3065 Fragments arranged by size: 3065 2366 997 865 406 298 249 125 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7034 882 Size: 617 5535 2219 Fragments arranged by size: 5535 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6162 493 Size: 711 4958 2702 Fragments arranged by size: 4958 2702 711 BsaBI GATnn'nnATC Cuts at: 5544 8365 5544 Size: 2821 5550 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6178 6560 7195 275 Size: 53 83 186 936 4645 382 635 1451 Fragments arranged by size: 4645 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2228 5088 6804 7202 7476 7623 8034 2228 Size: 2860 1716 398 274 147 411 2565 Fragments arranged by size: 2860 2565 1716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5201 5231 1178 Size: 30 165 30 3798 30 4318 Fragments arranged by size: 4318 3798 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4649 1186 Size: 3463 4908 BseYI C'CCAG_C Cuts at: 3094 4854 4997 7574 8070 3094 Size: 1760 143 2577 496 3395 Fragments arranged by size: 3395 2577 1760 496 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3138 4255 5075 1163 Size: 573 1402 1117 820 4459 Fragments arranged by size: 4459 1402 1117 820 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5821 6582 7610 1540 Size: 275 186 3820 761 1028 2301 Fragments arranged by size: 3820 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2544 4153 4637 6432 6517 7588 2544 Size: 1609 484 1795 85 1071 3327 Fragments arranged by size: 3327 1795 1609 1071 484 85 BsmI GAATG_Cn' Cuts at: 2962 5171 5882 5975 2962 Size: 2209 711 93 5358 Fragments arranged by size: 5358 2209 711 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2224 2544 2792 2888 3361 4153 4637 Size: 399 434 320 248 96 473 792 484 Cuts at: 4637 5690 6432 6517 7588 1391 Size: 1053 742 85 1071 2174 Fragments arranged by size: 2174 1071 1053 792 742 484 473 434 399 320 248 96 85 BspEI T'CCGG_A Cuts at: 8034 8034 Size: 8371 BspHI T'CATG_A Cuts at: 6260 7990 6260 Size: 1730 6641 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5127 5726 844 Size: 4283 599 3489 Fragments arranged by size: 4283 3489 599 BsrBI CCG'CTC Cuts at: 1394 1823 3091 6258 1394 Size: 429 1268 3167 3507 Fragments arranged by size: 3507 3167 1268 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4710 4788 5028 5608 6866 7040 65 Size: 1183 3462 78 240 580 1258 174 1396 Fragments arranged by size: 3462 1396 1258 1183 580 240 174 78 BsrFI r'CCGG_y Cuts at: 1700 1810 2228 7015 7202 1700 Size: 110 418 4787 187 2869 Fragments arranged by size: 4787 2869 418 187 110 BsrGI T'GTAC_A Cuts at: 96 4785 96 Size: 4689 3682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8371 BssSI C'ACGA_G Cuts at: 6431 7443 6431 Size: 1012 7359 BstAPI GCAn_nnn'nTGC Cuts at: 794 5177 794 Size: 4383 3988 BstBI TT'CG_AA Cuts at: 7175 7175 Size: 8371 BstEII G'GTnAC_C Cuts at: 1184 3625 1184 Size: 2441 5930 BstXI CCAn_nnnn'nTGG Cuts at: 3622 3748 5540 3622 Size: 126 1792 6453 Fragments arranged by size: 6453 1792 126 BstYI r'GATC_y Cuts at: 1972 4072 4270 4339 4969 5058 5701 6068 6453 Size: 2100 198 69 630 89 643 367 385 Cuts at: 6453 6470 7922 8008 8020 8366 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2100 1977 1452 643 630 385 367 346 198 89 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 3195 3195 Size: 8371 BtgI C'CryG_G Cuts at: 513 1065 3501 5505 513 Size: 552 2436 2004 3379 Fragments arranged by size: 3379 2436 2004 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7632 Fragments arranged by size: 7632 683 56 BtsI GCAGTG_nn' Cuts at: 5131 5887 6681 6701 5131 Size: 756 794 20 6801 Fragments arranged by size: 6801 794 756 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8336 DraI TTT'AAA Cuts at: 3663 3696 3882 5675 3663 Size: 33 186 1793 6359 Fragments arranged by size: 6359 1793 186 33 DraIII CAC_nnn'GTG Cuts at: 2107 2107 Size: 8371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3486 7378 809 Size: 507 2170 3892 1802 Fragments arranged by size: 3892 2170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2581 3108 3527 Size: 54 1425 50 275 18 751 527 419 Cuts at: 3527 5818 6707 8336 8 Size: 2291 889 1629 43 Fragments arranged by size: 2291 1629 1425 889 751 527 419 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5818 1537 Size: 275 4006 4090 Fragments arranged by size: 4090 4006 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4141 4667 5219 6301 1353 Size: 2788 526 552 1082 3423 Fragments arranged by size: 3423 2788 1082 552 526 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6958 7332 7478 387 Size: 1064 138 5369 374 146 1280 Fragments arranged by size: 5369 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2542 2542 Size: 8371 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3318 3894 4286 5635 6434 7031 7818 Size: 393 1686 576 392 1349 799 597 787 Cuts at: 7818 8086 1239 Size: 268 1524 Fragments arranged by size: 1686 1524 1349 799 787 597 576 393 392 268 EcoO109I rG'GnC_Cy Cuts at: 2154 3129 3358 3638 4196 5186 2154 Size: 975 229 280 558 990 5339 Fragments arranged by size: 5339 990 975 558 280 229 EcoRI G'AATT_C Cuts at: 4952 5680 6058 4952 Size: 728 378 7265 Fragments arranged by size: 7265 728 378 EcoRV GAT'ATC Cuts at: 3649 3649 Size: 8371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3595 4227 3595 Size: 632 7739 FspI TGC'GCA Cuts at: 5123 6877 5123 Size: 1754 6617 HaeII r_GCGC'y Cuts at: 1678 7518 1678 Size: 5840 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 3121 3153 4595 Size: 32 135 32 713 32 804 32 1442 Cuts at: 4595 4627 4954 4986 5537 5569 7012 7044 7086 Size: 32 327 32 551 32 1443 32 42 Cuts at: 7086 7118 1373 Size: 32 2626 Fragments arranged by size: 2626 1443 1442 804 713 551 327 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3007 5715 5961 669 Size: 988 281 1069 2708 246 3079 Fragments arranged by size: 3079 2708 1069 988 281 246 HindIII A'AGCT_T Cuts at: 748 2749 3876 748 Size: 2001 1127 5243 Fragments arranged by size: 5243 2001 1127 HpaI GTT'AAC Cuts at: 5961 5961 Size: 8371 KpnI G_GTAC'C Cuts at: 5696 5696 Size: 8371 MfeI C'AATT_G Cuts at: 2281 2636 3888 5970 2281 Size: 355 1252 2082 4682 Fragments arranged by size: 4682 2082 1252 355 MluI A'CGCG_T Cuts at: 8247 8247 Size: 8371 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2166 2997 3562 3678 4940 5448 7485 7669 Size: 861 831 565 116 1262 508 2037 184 Cuts at: 7669 1305 Size: 2007 Fragments arranged by size: 2037 2007 1262 861 831 565 508 184 116 MscI TGG'CCA Cuts at: 10 64 1489 1832 2583 3110 3529 10 Size: 54 1425 343 751 527 419 4852 Fragments arranged by size: 4852 1425 751 527 419 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3291 5033 5538 6329 6688 6847 518 Size: 648 2125 1742 505 791 359 159 2042 Fragments arranged by size: 2125 2042 1742 791 648 505 359 159 MspA1I CmG'CkG Cuts at: 1606 2982 3114 5787 6464 7612 7857 1606 Size: 1376 132 2673 677 1148 245 2120 Fragments arranged by size: 2673 2120 1376 1148 677 245 132 NcoI C'CATG_G Cuts at: 513 1065 3501 5505 513 Size: 552 2436 2004 3379 Fragments arranged by size: 3379 2436 2004 552 NdeI CA'TA_TG Cuts at: 387 4576 387 Size: 4189 4182 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8371 NotI GC'GGCC_GC Cuts at: 5818 5818 Size: 8371 NsiI A_TGCA'T Cuts at: 2388 2626 2713 2388 Size: 238 87 8046 Fragments arranged by size: 8046 238 87 NspI r_CATG'y Cuts at: 1132 1229 2209 2390 3935 4786 5729 7274 1132 Size: 97 980 181 1545 851 943 1545 2229 Fragments arranged by size: 2229 1545 1545 980 943 851 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8371 PciI A'CATG_T Cuts at: 3931 4782 7270 3931 Size: 851 2488 5032 Fragments arranged by size: 5032 2488 851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8371 PfoI T'CCnGG_A Cuts at: 4185 4185 Size: 8371 PmeI GTTT'AAAC Cuts at: 5675 5675 Size: 8371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 6440 6472 7953 7985 3710 Size: 32 2698 32 1481 32 4096 Fragments arranged by size: 4096 2698 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2154 3129 3638 4196 5186 2154 Size: 975 509 558 990 5339 Fragments arranged by size: 5339 990 975 558 509 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8371 PsiI TTA'TAA Cuts at: 2928 5941 2928 Size: 3013 5358 PspOMI G'GGCC_C Cuts at: 3357 3357 Size: 8371 PstI C_TGCA'G Cuts at: 830 5723 830 Size: 4893 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8371 PvuII CAG'CTG Cuts at: 2982 3114 2982 Size: 132 8239 SacI G_AGCT'C Cuts at: 2544 2544 Size: 8371 SalI G'TCGA_C Cuts at: 1936 3005 5713 1936 Size: 1069 2708 4594 Fragments arranged by size: 4594 2708 1069 SanDI GG'GwC_CC Cuts at: 3129 5186 3129 Size: 2057 6314 SapI GCTCTTCn'nnn_ Cuts at: 4141 4141 Size: 8371 SbfI CC_TGCA'GG Cuts at: 5723 5723 Size: 8371 ScaI AGT'ACT Cuts at: 6619 8331 6619 Size: 1712 6659 SexAI A'CCwGG_T Cuts at: 2505 2505 Size: 8371 SfcI C'TryA_G Cuts at: 826 1045 3154 5719 6854 7535 7726 826 Size: 219 2109 2565 1135 681 191 1471 Fragments arranged by size: 2565 2109 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8371 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8371 SmaI CCC'GGG Cuts at: 1872 5698 1872 Size: 3826 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2368 2461 3329 3454 3835 3860 Size: 197 926 425 93 868 125 381 25 Cuts at: 3860 4109 6475 7376 7638 820 Size: 249 2366 901 262 1553 Fragments arranged by size: 2366 1553 926 901 868 425 381 262 249 197 125 93 25 SnaBI TAC'GTA Cuts at: 493 6162 493 Size: 5669 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8371 SphI G_CATG'C Cuts at: 1132 5729 1132 Size: 4597 3774 SspI AAT'ATT Cuts at: 5 52 3736 3940 5632 6295 5 Size: 47 3684 204 1692 663 2081 Fragments arranged by size: 3684 2081 1692 663 204 47 StuI AGG'CCT Cuts at: 1517 2355 1517 Size: 838 7533 StyI C'CwwG_G Cuts at: 513 1065 3341 3441 3501 5505 5810 513 Size: 552 2276 100 60 2004 305 3074 Fragments arranged by size: 3074 2276 2004 552 305 100 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4521 6398 6415 6568 6753 7198 325 Size: 1255 2941 1877 17 153 185 445 1498 Fragments arranged by size: 2941 1877 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3205 4785 6617 Size: 274 80 33 51 1443 1228 1580 1832 Cuts at: 6617 8329 96 Size: 1712 138 Fragments arranged by size: 1832 1712 1580 1443 1228 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8331 8363 8331 Size: 32 8339 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8371 XbaI T'CTAG_A Cuts at: 5707 5707 Size: 8371 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2128 5041 2128 Size: 2913 5458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8371 XmaI C'CCGG_G Cuts at: 1870 5696 1870 Size: 3826 4545 XmnI GAAnn'nnTTC Cuts at: 2426 2826 3552 4776 6500 2426 Size: 400 726 1224 1724 4297 Fragments arranged by size: 4297 1724 1224 726 400 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6179 276 Size: 53 83 186 936 4645 2468 Fragments arranged by size: 4645 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI