################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BbvCI BcgI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HpaI KpnI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 97 GTAGAC GT^[AC][GT]AC AcsI 1462 AAATTC [GA]^AATT[CT] AhdI 499 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 1462 AAATTC [GA]^AATT[CT] AspEI 499 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 1840 GAAGAC GAAGAC(2/6) BciVI 1952 GGATAC GTATCC(6/5) BclI 1212 TGATCA T^GATCA BpuAI 1840 GAAGAC GAAGAC(2/6) BsaI 848 GGTCTC GGTCTC(1/5) BsgI 1591 CTGCAC GTGCAG(16/14) BsiEI 564 CGACCG CG[GA][CT]^CG BsmBI 418 GAGACG CGTCTC(1/5) BspHI 876 TCATGA T^CATGA BspMI 1227 ACCTGC ACCTGC(4/8) BstAPI 315 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1800 CCAGCGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 499 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1678 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1632 ATGCAT ATGCA^T FbaI 1212 TGATCA T^GATCA HindIII 1495 AAGCTT A^AGCTT HphI 863 TCACC GGTGA(8/7) KspI 431 CCGCGG CCGC^GG NaeI 366 GCCGGC GCC^GGC NgoMIV 366 GCCGGC G^CCGGC NsiI 1632 ATGCAT ATGCA^T PaeR7I 259 CTCGAG C^TCGAG PstI 125 CTGCAG CTGCA^G RcaI 876 TCATGA T^CATGA SacII 431 CCGCGG CCGC^GG SfcI 125 CTGCAG C^T[GA][CT]AG SgrAI 365 CGCCGGCG C[GA]^CCGG[CT]G SmaI 236 CCCGGG CCC^GGG Tsp45I 1309 GTCAC ^GT[GC]AC XcmI 2017 CCAGCCTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 259 CTCGAG C^TCGAG XmaCI 236 CCCGGG C^CCGGG XmaI 236 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 22 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 289 CAGCAGCTG ApaI 105 GGGCCC GGGCC^C 132 GGGCCC AvaI 236 CCCGGG C^[CT]CG[GA]G 259 CTCGAG BalI 519 TGGCCA TGG^CCA 611 TGGCCA BbeI 363 GGCGCC GGCGC^C 369 GGCGCC BpmI 310 CTGGAG CTGGAG(16/14) 943 CTGGAG BsmI 882 GAATGC GAATGC(1/-1) 1273 GAATGC BsoBI 236 CCCGGG C^[CT]CG[GA]G 259 CTCGAG BsrBI 1746 CCGCTC CCGCTC(-3/-3) 283 GAGCGG BsrDI 1144 CATTGC GCAATG(2/0) 2083 GCAATG BsrFI 366 GCCGGC [GA]^CCGG[CT] 566 ACCGGC BssSI 742 CACGAG CACGAG(-5/-1) 1647 CACGAG BstYI 1200 AGATCC [GA]^GATC[CT] 1674 AGATCC Cfr10I 366 GCCGGC [GA]^CCGG[CT] 566 ACCGGC DsaI 404 CCGTGG C^C[GA][CT]GG 431 CCGCGG HincII 673 GTCAAC GT[CT]^[GA]AC 1756 GTTGAC HindII 673 GTCAAC GT[CT]^[GA]AC 1756 GTTGAC KasI 363 GGCGCC G^GCGCC 369 GGCGCC MaeII 549 ACGT A^CGT 677 ACGT MaeIII 1309 GTCAC ^GT[ACGT]AC 1610 GTTAC MflI 1200 AGATCC [GA]^GATC[CT] 1674 AGATCC MluNI 519 TGGCCA TGG^CCA 611 TGGCCA MscI 519 TGGCCA TGG^CCA 611 TGGCCA NarI 363 GGCGCC GG^CGCC 369 GGCGCC NspI 907 GCATGT [GA]CATG^[CT] 1661 GCATGT PpuMI 181 GGGACCT [GA]G^G[AT]CC[CT] 2089 AGGTCCT SfoI 363 GGCGCC GGC^GCC 369 GGCGCC SmlI 259 CTCGAG C^T[CT][GA]AG 790 CTCAAG XhoII 1200 AGATCC [GA]^GATC[CT] 1674 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BlpI BmyI Bpu1102I BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I MaeI MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I