(Circular) (Six-base) MAPSORT of: pFN21AE4219.seq Check: 419 from: 1 to: 6859 pFN21AE4219 6859 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4669 278 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2103 4202 1937 Size: 166 2099 4594 Fragments arranged by size: 4594 2099 166 Acc65I G'GTAC_C Cuts at: 4180 4180 Size: 6859 AclI AA'CG_TT Cuts at: 1563 4158 4986 5359 1563 Size: 2595 828 373 3063 Fragments arranged by size: 3063 2595 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3506 3769 4922 6306 6574 1632 Size: 1874 263 1153 1384 268 1917 Fragments arranged by size: 1917 1874 1384 1153 268 263 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6662 AflIII A'CryG_T Cuts at: 1203 5758 6735 1203 Size: 4555 977 1327 Fragments arranged by size: 4555 1327 977 AgeI A'CCGG_T Cuts at: 5690 5690 Size: 6859 AhdI GACnn_n'nnGTC Cuts at: 2509 5588 2509 Size: 3079 3780 AleI CACnn'nnGTG Cuts at: 1166 2456 1166 Size: 1290 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2228 1184 Size: 1044 5815 AlwNI CAG_nnn'CTG Cuts at: 2032 2299 4602 6174 2032 Size: 267 2303 1572 2717 Fragments arranged by size: 2717 2303 1572 267 ApaI G_GGCC'C Cuts at: 2114 2141 2114 Size: 27 6832 ApaLI G'TGCA_C Cuts at: 4916 6072 4916 Size: 1156 5703 ApoI r'AATT_y Cuts at: 1493 3467 4168 4394 4546 6655 1493 Size: 1974 701 226 152 2109 1697 Fragments arranged by size: 2109 1974 1697 701 226 152 AseI AT'TA_AT Cuts at: 160 1032 5413 160 Size: 872 4381 1606 Fragments arranged by size: 4381 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 AvaI C'yCGr_G Cuts at: 1870 1943 2241 2264 4184 1870 Size: 73 298 23 1920 4545 Fragments arranged by size: 4545 1920 298 73 23 BamHI G'GATC_C Cuts at: 4189 4556 4189 Size: 367 6492 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2368 2374 2405 4180 5635 Size: 325 217 627 581 6 31 1775 1455 Cuts at: 5635 618 Size: 1842 Fragments arranged by size: 1842 1775 1455 627 581 325 217 31 6 BanII G_rGCy'C Cuts at: 1391 2114 2141 2641 2955 3459 3882 4072 4178 Size: 723 27 500 314 504 423 190 106 Cuts at: 4178 1391 Size: 4072 Fragments arranged by size: 4072 723 504 500 423 314 190 106 27 BbeI G_GCGC'C Cuts at: 2372 2378 2372 Size: 6 6853 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3852 928 Size: 971 1953 3935 Fragments arranged by size: 3935 1953 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5050 5084 1903 Size: 34 3113 34 3678 Fragments arranged by size: 3678 3113 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3951 4751 5961 3951 Size: 800 1210 4849 Fragments arranged by size: 4849 1210 800 BclI T'GATC_A Cuts at: 1548 3217 1548 Size: 1669 5190 BfrBI ATG'CAT Cuts at: 3639 3639 Size: 6859 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2438 2645 2703 Size: 107 122 71 631 605 766 207 58 Cuts at: 2703 5470 136 Size: 2767 1525 Fragments arranged by size: 2767 1525 766 631 605 207 122 107 71 58 BglII A'GATC_T Cuts at: 6854 6854 Size: 6859 BlpI GC'TnA_GC Cuts at: 2033 2307 2690 3876 4071 4276 2033 Size: 274 383 1186 195 205 4616 Fragments arranged by size: 4616 1186 383 274 205 195 Bme1580I G_kGCm'C Cuts at: 1790 2114 2141 4920 6076 1790 Size: 324 27 2779 1156 2573 Fragments arranged by size: 2779 2573 1156 324 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6859 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2631 4590 5548 Size: 416 53 83 399 24 1214 1959 958 Cuts at: 5548 442 Size: 1753 Fragments arranged by size: 1959 1753 1214 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6859 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4091 Size: 6859 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2969 5519 1239 Size: 1097 633 2550 2579 Fragments arranged by size: 2579 2550 1097 633 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2780 4984 5849 6147 2780 Size: 2204 865 298 3492 Fragments arranged by size: 3492 2204 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2859 5522 882 Size: 617 1360 2663 2219 Fragments arranged by size: 2663 2219 1360 617 BsaAI yAC'GTr Cuts at: 493 1204 4650 493 Size: 711 3446 2702 Fragments arranged by size: 3446 2702 711 BsaBI GATnn'nnATC Cuts at: 6853 6853 Size: 6859 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 2237 2369 2375 Size: 53 83 186 936 696 8 132 6 Cuts at: 2375 3993 4666 5048 5683 275 Size: 1618 673 382 635 1451 Fragments arranged by size: 1618 1451 936 696 673 635 382 186 132 83 53 8 6 BsaWI w'CCGG_w Cuts at: 5292 5690 5964 6111 6522 5292 Size: 398 274 147 411 5629 Fragments arranged by size: 5629 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3065 3095 3669 3699 1178 Size: 30 165 30 1662 30 574 30 4338 Fragments arranged by size: 4338 1662 574 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 3086 3089 3467 1186 Size: 1504 396 3 378 4578 Fragments arranged by size: 4578 1504 396 378 3 BseYI C'CCAG_C Cuts at: 2648 2722 3016 4021 6062 6558 2648 Size: 74 294 1005 2041 496 2949 Fragments arranged by size: 2949 2041 1005 496 294 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3581 1163 Size: 573 1845 4441 Fragments arranged by size: 4441 1845 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2572 4309 5070 6098 1540 Size: 275 186 571 1737 761 1028 2301 Fragments arranged by size: 2301 1737 1028 761 571 275 186 BsiHKAI G_wGCw'C Cuts at: 2691 2955 3459 3795 3882 4072 4920 5005 6076 Size: 264 504 336 87 190 848 85 1071 Cuts at: 6076 2691 Size: 3474 Fragments arranged by size: 3474 1071 848 504 336 264 190 87 85 BsmI GAATG_Cn' Cuts at: 2893 3284 4370 4463 2893 Size: 391 1086 93 5289 Fragments arranged by size: 5289 1086 391 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2417 2417 Size: 6859 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2141 2641 2691 2955 3459 3795 Size: 399 324 27 500 50 264 504 336 Cuts at: 3795 3882 4072 4178 4920 5005 6076 1391 Size: 87 190 106 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 500 399 336 324 264 190 106 87 85 50 27 BspEI T'CCGG_A Cuts at: 6522 6522 Size: 6859 BspHI T'CATG_A Cuts at: 2881 4748 6478 2881 Size: 1867 1730 3262 Fragments arranged by size: 3262 1867 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3241 4214 844 Size: 2397 973 3489 Fragments arranged by size: 3489 2397 973 BsrBI CCG'CTC Cuts at: 1394 1823 2290 3753 4746 1394 Size: 429 467 1463 993 3507 Fragments arranged by size: 3507 1463 993 467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3148 4095 5354 5528 65 Size: 1183 1900 947 1259 174 1396 Fragments arranged by size: 1900 1396 1259 1183 947 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2371 2571 5503 5690 1700 Size: 110 561 200 2932 187 2869 Fragments arranged by size: 2932 2869 561 200 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6859 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6859 BssSI C'ACGA_G Cuts at: 2747 3652 4919 5931 2747 Size: 905 1267 1012 3675 Fragments arranged by size: 3675 1267 1012 905 BstAPI GCAn_nnn'nTGC Cuts at: 794 2326 794 Size: 1532 5327 BstBI TT'CG_AA Cuts at: 5663 5663 Size: 6859 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6859 BstXI CCAn_nnnn'nTGG Cuts at: 3812 3812 Size: 6859 BstYI r'GATC_y Cuts at: 1972 3205 3679 4189 4556 4941 4958 6410 6496 Size: 1233 474 510 367 385 17 1452 86 Cuts at: 6496 6508 6854 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1233 510 474 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2409 2436 513 Size: 552 939 405 27 4936 Fragments arranged by size: 4936 939 552 405 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6120 Fragments arranged by size: 6120 683 56 BtsI GCAGTG_nn' Cuts at: 2394 2789 2891 4375 5169 5189 2394 Size: 395 102 1484 794 20 4064 Fragments arranged by size: 4064 1484 794 395 102 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2516 2551 529 Size: 35 1952 35 4837 Fragments arranged by size: 4837 1952 35 35 DraI TTT'AAA Cuts at: 4163 4163 Size: 6859 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5866 809 Size: 507 4550 1802 Fragments arranged by size: 4550 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2448 2524 2616 Size: 54 1425 50 275 18 618 76 92 Cuts at: 2616 3748 4306 5195 6824 8 Size: 1132 558 889 1629 43 Fragments arranged by size: 1629 1425 1132 889 618 558 275 92 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4306 1537 Size: 275 2494 4090 Fragments arranged by size: 4090 2494 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 3063 3504 4789 1353 Size: 1062 648 441 1285 3423 Fragments arranged by size: 3423 1285 1062 648 441 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2090 2354 2432 2593 2912 5446 Size: 1064 138 501 264 78 161 319 2534 Cuts at: 5446 5820 5966 387 Size: 374 146 1280 Fragments arranged by size: 2534 1280 1064 501 374 319 264 161 146 138 78 EcoICRI GAG'CTC Cuts at: 2953 3457 3880 4070 2953 Size: 504 423 190 5742 Fragments arranged by size: 5742 504 423 190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2969 3506 3769 4922 5519 6306 Size: 393 704 633 537 263 1153 597 787 Cuts at: 6306 6574 1239 Size: 268 1524 Fragments arranged by size: 1524 1153 787 704 633 597 537 393 268 263 EcoNI CCTnn'n_nnAGG Cuts at: 3687 3687 Size: 6859 EcoO109I rG'GnC_Cy Cuts at: 2111 2137 2138 2187 4095 2111 Size: 26 1 49 1908 4875 Fragments arranged by size: 4875 1908 49 26 1 EcoRI G'AATT_C Cuts at: 4168 4546 4168 Size: 378 6481 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3419 3799 3419 Size: 380 6479 FspI TGC'GCA Cuts at: 5365 5365 Size: 6859 HaeII r_GCGC'y Cuts at: 1678 2366 2372 2378 2578 2776 3784 6006 1678 Size: 688 6 6 200 198 1008 2222 2531 Fragments arranged by size: 2531 2222 1008 688 200 198 6 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 3077 3109 3669 Size: 32 135 32 923 32 550 32 560 Cuts at: 3669 3701 3737 3769 3949 3981 5500 5532 5574 Size: 32 36 32 180 32 1519 32 42 Cuts at: 5574 5606 1373 Size: 32 2626 Fragments arranged by size: 2626 1519 923 560 550 180 135 42 36 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2680 3763 4203 4449 669 Size: 988 281 742 1083 440 246 3079 Fragments arranged by size: 3079 1083 988 742 440 281 246 HindIII A'AGCT_T Cuts at: 748 3500 748 Size: 2752 4107 HpaI GTT'AAC Cuts at: 4449 4449 Size: 6859 KasI G'GCGC_C Cuts at: 2368 2374 2368 Size: 6 6853 KpnI G_GTAC'C Cuts at: 4184 4184 Size: 6859 MfeI C'AATT_G Cuts at: 4458 4458 Size: 6859 MluI A'CGCG_T Cuts at: 6735 6735 Size: 6859 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3063 5973 6157 1305 Size: 1758 2910 184 2007 Fragments arranged by size: 2910 2007 1758 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2526 2618 10 Size: 54 1425 343 694 92 4251 Fragments arranged by size: 4251 1425 694 343 92 54 MslI CAynn'nnrTG Cuts at: 518 1166 2456 2886 3897 4817 5176 5335 518 Size: 648 1290 430 1011 920 359 159 2042 Fragments arranged by size: 2042 1290 1011 920 648 430 359 159 MspA1I CmG'CkG Cuts at: 1606 2093 2299 2314 2323 2438 2671 2694 3808 Size: 487 206 15 9 115 233 23 1114 Cuts at: 3808 4275 4952 6100 6345 1606 Size: 467 677 1148 245 2120 Fragments arranged by size: 2120 1148 1114 677 487 467 245 233 206 115 23 15 9 NaeI GCC'GGC Cuts at: 2373 2373 Size: 6859 NarI GG'CG_CC Cuts at: 2369 2375 2369 Size: 6 6853 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5368 Fragments arranged by size: 5368 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6859 NgoMIV G'CCGG_C Cuts at: 2371 2371 Size: 6859 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6859 NotI GC'GGCC_GC Cuts at: 4306 4306 Size: 6859 NsiI A_TGCA'T Cuts at: 3641 3641 Size: 6859 NspI r_CATG'y Cuts at: 1132 1229 2916 3670 4217 5762 1132 Size: 97 1687 754 547 1545 2229 Fragments arranged by size: 2229 1687 1545 754 547 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6859 PciI A'CATG_T Cuts at: 5758 5758 Size: 6859 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6859 PmeI GTTT'AAAC Cuts at: 4163 4163 Size: 6859 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4928 4960 6441 6473 4928 Size: 32 1481 32 5314 Fragments arranged by size: 5314 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2187 4095 2187 Size: 1908 4951 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6859 PsiI TTA'TAA Cuts at: 4429 4429 Size: 6859 PspOMI G'GGCC_C Cuts at: 2110 2137 2110 Size: 27 6832 PspXI vC'TCGA_Gb Cuts at: 2264 2264 Size: 6859 PstI C_TGCA'G Cuts at: 830 2134 4211 830 Size: 1304 2077 3478 Fragments arranged by size: 3478 2077 1304 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6859 PvuII CAG'CTG Cuts at: 2299 2314 2323 2299 Size: 15 9 6835 Fragments arranged by size: 6835 15 9 SacI G_AGCT'C Cuts at: 2955 3459 3882 4072 2955 Size: 504 423 190 5742 Fragments arranged by size: 5742 504 423 190 SacII CC_GC'GG Cuts at: 2439 2439 Size: 6859 SalI G'TCGA_C Cuts at: 1936 4201 1936 Size: 2265 4594 SbfI CC_TGCA'GG Cuts at: 4211 4211 Size: 6859 ScaI AGT'ACT Cuts at: 5107 6819 5107 Size: 1712 5147 SfcI C'TryA_G Cuts at: 826 1045 2130 4207 5342 6023 6214 826 Size: 219 1085 2077 1135 681 191 1471 Fragments arranged by size: 2077 1471 1135 1085 681 219 191 SfoI GGC'GCC Cuts at: 2370 2376 2370 Size: 6 6853 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 SgrAI Cr'CCGG_yG Cuts at: 1700 2371 1700 Size: 671 6188 SmaI CCC'GGG Cuts at: 1872 2243 4186 1872 Size: 371 1943 4545 Fragments arranged by size: 4545 1943 371 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 2795 4963 5864 6126 820 Size: 197 926 321 531 2168 901 262 1553 Fragments arranged by size: 2168 1553 926 901 531 321 262 197 SnaBI TAC'GTA Cuts at: 493 4650 493 Size: 4157 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6859 SphI G_CATG'C Cuts at: 1132 4217 1132 Size: 3085 3774 SspI AAT'ATT Cuts at: 5 52 4783 5 Size: 47 4731 2081 Fragments arranged by size: 4731 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6859 StyI C'CwwG_G Cuts at: 513 1065 2004 2232 2871 3673 4056 4298 513 Size: 552 939 228 639 802 383 242 3074 Fragments arranged by size: 3074 939 802 639 552 383 242 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2133 2841 4886 4903 5056 5241 5686 Size: 1255 553 708 2045 17 153 185 445 Cuts at: 5686 325 Size: 1498 Fragments arranged by size: 2045 1498 1255 708 553 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2799 3563 5105 Size: 274 80 33 51 1443 822 764 1542 Cuts at: 5105 6817 96 Size: 1712 138 Fragments arranged by size: 1712 1542 1443 822 764 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6819 6851 6819 Size: 32 6827 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6859 XbaI T'CTAG_A Cuts at: 4195 4195 Size: 6859 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4029 4029 Size: 6859 XhoI C'TCGA_G Cuts at: 1943 2264 1943 Size: 321 6538 XmaI C'CCGG_G Cuts at: 1870 2241 4184 1870 Size: 371 1943 4545 Fragments arranged by size: 4545 1943 371 XmnI GAAnn'nnTTC Cuts at: 4988 4988 Size: 6859 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4667 276 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI PacI PfoI PmlI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI