################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AocI ApaI AscI Asp718I AspEI AvaI AviII AvrII BalI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspLU11I BsrBI BssHII BstXI Bsu36I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoRV FbaI FseI FspI HgaI HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcyI 1547 GGCGCC G[GA]^CG[CT]C Alw44I 3007 GTGCAC G^TGCAC AlwNI 3314 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1613 AGCGCT AGC^GCT ApaLI 3007 GTGCAC G^TGCAC AspI 2854 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 1547 GGCGCC G^G[CT][GA]CC BbeI 1547 GGCGCC GGCGC^C BglII 2391 AGATCT A^GATCT BlpI 1295 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1295 GCTCAGC GC^T[ACGT]AGC BsaHI 1547 GGCGCC G[GA]^CG[CT]C BsmBI 13 GAGACG CGTCTC(1/5) BsmI 906 GCATTC GAATGC(1/-1) Bsp1407I 21 TGTACA T^GTACA BspHI 2961 TCATGA T^CATGA BsrFI 2034 GCCGGC [GA]^CCGG[CT] BsrGI 21 TGTACA T^GTACA Bst1107I 778 GTATAC GTA^TAC BstAPI 3230 GCATTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3165 TTCGAA TT^CGAA BstEII 2689 GGTGACC G^GT[ACGT]ACC BstPI 2689 GGTGACC G^GT[ACGT]ACC BstZ17I 778 GTATAC GTA^TAC CelII 1295 GCTCAGC GC^T[ACGT]AGC Cfr10I 2034 GCCGGC [GA]^CCGG[CT] DraIII 2414 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1613 AGCGCT AGC^GCT EcoNI 1257 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2689 GGTGACC G^GT[ACGT]ACC EcoRI 3423 GAATTC G^AATTC Hin1I 1547 GGCGCC G[GA]^CG[CT]C HincII 2176 GTTGAC GT[CT]^[GA]AC HindII 2176 GTTGAC GT[CT]^[GA]AC KasI 1547 GGCGCC G^GCGCC NaeI 2034 GCCGGC GCC^GGC NarI 1547 GGCGCC GG^CGCC NcoI 1496 CCATGG C^CATGG NgoMIV 2034 GCCGGC G^CCGGC NspV 3165 TTCGAA TT^CGAA PflFI 2854 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 2407 CTGCAG CTGCA^G RcaI 2961 TCATGA T^CATGA ScaI 1217 AGTACT AGT^ACT SexAI 448 ACCTGGT A^CC[AT]GGT SfoI 1547 GGCGCC GGC^GCC SfuI 3165 TTCGAA TT^CGAA SmlI 631 CTTGAG C^T[CT][GA]AG SspBI 21 TGTACA T^GTACA SspI 646 AATATT AAT^ATT Tth111I 2854 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 196 CCAGTGGATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 778 GTATAC GT^[AC][GT]AC 2284 GTCTAC AccII 2320 CGCG CG^CG 2456 CGCG AseI 649 ATTAAT AT^TAAT 3271 ATTAAT AsnI 649 ATTAAT AT^TAAT 3271 ATTAAT Asp700I 1066 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3376 GAAAGATTTC AvaII 734 GGACC G^G[AT]CC 1173 GGTCC BamHI 1083 GGATCC G^GATCC 1187 GGATCC BanII 1138 GAGCTC G[GA]GC[CT]^C 1293 GAGCTC BfaI 115 CTAG C^TAG 1867 CTAG BsaI 896 GGTCTC GGTCTC(1/5) 2467 GGTCTC BssSI 1290 CACGAG CACGAG(-5/-1) 2429 CTCGTG BstUI 2320 CGCG CG^CG 2456 CGCG EcoT22I 392 ATGCAT ATGCA^T 1851 ATGCAT FauI 2454 CCCGC CCCGC(4/6) 918 GCGGG HindIII 572 AAGCTT A^AGCTT 2611 AAGCTT MaeI 115 CTAG C^TAG 1867 CTAG MvnI 2320 CGCG CG^CG 2456 CGCG NsiI 392 ATGCAT ATGCA^T 1851 ATGCAT PflMI 196 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 998 CCATCCTTTGG PshAI 544 GACGTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1535 GACCTGTGTC PshBI 649 ATTAAT AT^TAAT 3271 ATTAAT SacI 1138 GAGCTC GAGCT^C 1293 GAGCTC SapI 1135 GAAGAGC GCTCTTC(1/4) 3088 GAAGAGC SphI 1849 GCATGC GCATG^C 2199 GCATGC Van91I 196 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 998 CCATCCTTTGG XmnI 1066 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3376 GAAAGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI BbsI BbvI BciVI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII