(Linear) (Six-base) MAPSORT of: KIEE3704.seq Check: 8395 from: 1 to: 3429 KIEE3704.orf With 182 enzymes: SgfI * July 3, 2012 18:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2142 3429 Size: 2142 1287 AccI GT'mk_AC Cuts at: 0 779 2285 3429 Size: 779 1506 1144 Fragments arranged by size: 1506 1144 779 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 803 1052 1279 2421 2479 2629 2889 Size: 181 622 249 227 1142 58 150 260 Cuts at: 2889 3429 Size: 540 Fragments arranged by size: 1142 622 540 260 249 227 181 150 58 AfeI AGC'GCT Cuts at: 0 1615 3429 Size: 1615 1814 AleI CACnn'nnGTG Cuts at: 0 1627 3004 3014 3429 Size: 1627 1377 10 415 Fragments arranged by size: 1627 1377 415 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 853 885 3429 Size: 853 32 2544 Fragments arranged by size: 2544 853 32 AlwNI CAG_nnn'CTG Cuts at: 0 3319 3429 Size: 3319 110 ApaLI G'TGCA_C Cuts at: 0 3007 3429 Size: 3007 422 ApoI r'AATT_y Cuts at: 0 165 791 2570 3423 3429 Size: 165 626 1779 853 6 Fragments arranged by size: 1779 853 626 165 6 AseI AT'TA_AT Cuts at: 0 650 3272 3429 Size: 650 2622 157 Fragments arranged by size: 2622 650 157 BamHI G'GATC_C Cuts at: 0 1083 1187 3429 Size: 1083 104 2242 Fragments arranged by size: 2242 1083 104 BanI G'GyrC_C Cuts at: 0 1547 3429 Size: 1547 1882 BanII G_rGCy'C Cuts at: 0 1142 1297 3429 Size: 1142 155 2132 Fragments arranged by size: 2132 1142 155 BbeI G_GCGC'C Cuts at: 0 1551 3429 Size: 1551 1878 BbsI GAAGACnn'nnnn_ Cuts at: 0 239 485 2549 2606 3155 3429 Size: 239 246 2064 57 549 274 Fragments arranged by size: 2064 549 274 246 239 57 BciVI GTATCCnnnnn_n' Cuts at: 0 574 872 1753 3429 Size: 574 298 881 1676 Fragments arranged by size: 1676 881 574 298 BfrBI ATG'CAT Cuts at: 0 394 1853 3429 Size: 394 1459 1576 Fragments arranged by size: 1576 1459 394 BglII A'GATC_T Cuts at: 0 2391 3429 Size: 2391 1038 BlpI GC'TnA_GC Cuts at: 0 1296 3429 Size: 1296 2133 Bme1580I G_kGCm'C Cuts at: 0 2162 3011 3429 Size: 2162 849 418 Fragments arranged by size: 2162 849 418 BmrI ACTGGGnnnn_n' Cuts at: 0 190 1230 1280 2254 3429 Size: 190 1040 50 974 1175 Fragments arranged by size: 1175 1040 974 190 50 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 914 1781 3429 Size: 914 867 1648 Fragments arranged by size: 1648 914 867 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 544 853 884 1751 2188 2416 3086 3429 Size: 544 309 31 867 437 228 670 343 Fragments arranged by size: 867 670 544 437 343 309 228 31 Bpu10I CC'TnA_GC Cuts at: 0 2482 3429 Size: 2482 947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 652 3429 Size: 652 2777 BsaI GGTCTCn'nnnn_ Cuts at: 0 902 2473 3429 Size: 902 1571 956 Fragments arranged by size: 1571 956 902 BsaHI Gr'CG_yC Cuts at: 0 1548 3429 Size: 1548 1881 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1591 1621 3429 Size: 1591 30 1808 Fragments arranged by size: 1808 1591 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 531 906 1612 1792 2107 2330 3429 Size: 531 375 706 180 315 223 1099 Fragments arranged by size: 1099 706 531 375 315 223 180 BseYI C'CCAG_C Cuts at: 0 3037 3429 Size: 3037 392 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 560 1651 1674 2103 2260 3429 Size: 560 1091 23 429 157 1169 Fragments arranged by size: 1169 1091 560 429 157 23 BsiHKAI G_wGCw'C Cuts at: 0 1142 1297 1505 3011 3429 Size: 1142 155 208 1506 418 Fragments arranged by size: 1506 1142 418 208 155 BsmI GAATG_Cn' Cuts at: 0 906 3429 Size: 906 2523 BsmBI CGTCTCn'nnnn_ Cuts at: 0 7 3429 Size: 7 3422 Bsp1286I G_dGCh'C Cuts at: 0 1142 1297 1505 2162 3011 3429 Size: 1142 155 208 657 849 418 Fragments arranged by size: 1142 849 657 418 208 155 BspHI T'CATG_A Cuts at: 0 2961 3429 Size: 2961 468 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 201 1849 2142 3268 3429 Size: 201 1648 293 1126 161 Fragments arranged by size: 1648 1126 293 201 161 BsrDI GCAATG_nn' Cuts at: 0 782 1725 2849 3429 Size: 782 943 1124 580 Fragments arranged by size: 1124 943 782 580 BsrFI r'CCGG_y Cuts at: 0 2034 3429 Size: 2034 1395 BsrGI T'GTAC_A Cuts at: 0 21 3429 Size: 21 3408 BssSI C'ACGA_G Cuts at: 0 1290 2429 3429 Size: 1290 1139 1000 Fragments arranged by size: 1290 1139 1000 BstAPI GCAn_nnn'nTGC Cuts at: 0 3236 3429 Size: 3236 193 BstBI TT'CG_AA Cuts at: 0 3166 3429 Size: 3166 263 BstEII G'GTnAC_C Cuts at: 0 2689 3429 Size: 2689 740 BstYI r'GATC_y Cuts at: 0 1083 1158 1187 2003 2243 2391 3429 Size: 1083 75 29 816 240 148 1038 Fragments arranged by size: 1083 1038 816 240 148 75 29 BstZ17I GTA'TAC Cuts at: 0 780 3429 Size: 780 2649 BtgI C'CryG_G Cuts at: 0 729 873 1496 3429 Size: 729 144 623 1933 Fragments arranged by size: 1933 729 623 144 BtsI GCAGTG_nn' Cuts at: 0 1760 3429 Size: 1760 1669 DraIII CAC_nnn'GTG Cuts at: 0 2419 3429 Size: 2419 1010 EarI CTCTTCn'nnn_ Cuts at: 0 662 700 965 1130 1778 2963 3083 3356 Size: 662 38 265 165 648 1185 120 273 Cuts at: 3356 3429 Size: 73 Fragments arranged by size: 1185 662 648 273 265 165 120 73 38 EcoICRI GAG'CTC Cuts at: 0 1140 1295 3429 Size: 1140 155 2134 Fragments arranged by size: 2134 1140 155 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 544 803 853 884 1052 1279 1751 Size: 181 363 259 50 31 168 227 472 Cuts at: 1751 2188 2416 2421 2479 2629 2889 3086 3429 Size: 437 228 5 58 150 260 197 343 Fragments arranged by size: 472 437 363 343 260 259 228 227 197 181 168 150 58 50 31 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 1261 3429 Size: 1261 2168 EcoRI G'AATT_C Cuts at: 0 3423 3429 Size: 3423 6 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 513 994 3429 Size: 513 481 2435 Fragments arranged by size: 2435 513 481 HaeII r_GCGC'y Cuts at: 0 1551 1617 2102 3429 Size: 1551 66 485 1327 Fragments arranged by size: 1551 1327 485 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 109 141 1279 1311 1385 1417 1647 1679 Size: 109 32 1138 32 74 32 230 32 Cuts at: 1679 2383 2415 2475 2507 3429 Size: 704 32 60 32 922 Fragments arranged by size: 1138 922 704 230 109 74 60 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2178 3429 Size: 2178 1251 HindIII A'AGCT_T Cuts at: 0 572 2611 3429 Size: 572 2039 818 Fragments arranged by size: 2039 818 572 KasI G'GCGC_C Cuts at: 0 1547 3429 Size: 1547 1882 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 27 2108 2519 2831 2864 3429 Size: 27 2081 411 312 33 565 Fragments arranged by size: 2081 565 411 312 33 27 MslI CAynn'nnrTG Cuts at: 0 721 1627 2020 3004 3014 3429 Size: 721 906 393 984 10 415 Fragments arranged by size: 984 906 721 415 393 10 MspA1I CmG'CkG Cuts at: 0 1300 1648 2060 2449 2721 3429 Size: 1300 348 412 389 272 708 Fragments arranged by size: 1300 708 412 389 348 272 NaeI GCC'GGC Cuts at: 0 2036 3429 Size: 2036 1393 NarI GG'CG_CC Cuts at: 0 1548 3429 Size: 1548 1881 NcoI C'CATG_G Cuts at: 0 1496 3429 Size: 1496 1933 NgoMIV G'CCGG_C Cuts at: 0 2034 3429 Size: 2034 1395 NsiI A_TGCA'T Cuts at: 0 396 1855 3429 Size: 396 1459 1574 Fragments arranged by size: 1574 1459 396 NspI r_CATG'y Cuts at: 0 1446 1853 2203 3206 3429 Size: 1446 407 350 1003 223 Fragments arranged by size: 1446 1003 407 350 223 PasI CC'CwG_GG Cuts at: 0 225 3429 Size: 225 3204 PflMI CCAn_nnn'nTGG Cuts at: 0 202 1004 3429 Size: 202 802 2425 Fragments arranged by size: 2425 802 202 PfoI T'CCnGG_A Cuts at: 0 857 3429 Size: 857 2572 PshAI GACnn'nnGTC Cuts at: 0 548 1539 3429 Size: 548 991 1890 Fragments arranged by size: 1890 991 548 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 401 433 3429 Size: 401 32 2996 Fragments arranged by size: 2996 401 32 PstI C_TGCA'G Cuts at: 0 2411 3429 Size: 2411 1018 PvuII CAG'CTG Cuts at: 0 1300 2060 2449 3429 Size: 1300 760 389 980 Fragments arranged by size: 1300 980 760 389 SacI G_AGCT'C Cuts at: 0 1142 1297 3429 Size: 1142 155 2132 Fragments arranged by size: 2132 1142 155 SapI GCTCTTCn'nnn_ Cuts at: 0 1130 3083 3429 Size: 1130 1953 346 Fragments arranged by size: 1953 1130 346 ScaI AGT'ACT Cuts at: 0 1219 3429 Size: 1219 2210 SexAI A'CCwGG_T Cuts at: 0 448 3429 Size: 448 2981 SfcI C'TryA_G Cuts at: 0 418 1143 2407 3429 Size: 418 725 1264 1022 Fragments arranged by size: 1264 1022 725 418 SfoI GGC'GCC Cuts at: 0 1549 3429 Size: 1549 1880 SmlI C'TyrA_G Cuts at: 0 631 3429 Size: 631 2798 SphI G_CATG'C Cuts at: 0 1853 2203 3429 Size: 1853 350 1226 Fragments arranged by size: 1853 1226 350 SspI AAT'ATT Cuts at: 0 648 3429 Size: 648 2781 StuI AGG'CCT Cuts at: 0 85 464 2684 2927 2955 3429 Size: 85 379 2220 243 28 474 Fragments arranged by size: 2220 474 379 243 85 28 StyI C'CwwG_G Cuts at: 0 473 1191 1496 2685 3429 Size: 473 718 305 1189 744 Fragments arranged by size: 1189 744 718 473 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 194 1376 3429 Size: 194 1182 2053 Fragments arranged by size: 2053 1182 194 TatI w'GTAC_w Cuts at: 0 21 1217 3018 3429 Size: 21 1196 1801 411 Fragments arranged by size: 1801 1196 411 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1179 1211 1591 1623 2415 2447 3429 Size: 1179 32 380 32 792 32 982 Fragments arranged by size: 1179 982 792 380 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2857 3429 Size: 2857 572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 203 3429 Size: 203 3226 XmnI GAAnn'nnTTC Cuts at: 0 1070 3380 3429 Size: 1070 2310 49 Fragments arranged by size: 2310 1070 49 Enzymes that do cut: AarI AccI AcuI AfeI AleI AloI AlwNI ApaLI ApoI AseI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtsI DraIII EarI EcoICRI Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PshAI PsrI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI ApaI AscI AsiSI AvaI AvrII BaeI BbvCI BcgI BclI BglI BmgBI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BssHII BstXI Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI EcoO109I EcoRV FseI FspI FspAI HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XbaI XhoI XmaI ZraI