################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BcgI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrFI BssHII BssSI Bst1107I BstAPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 2531 GTCTAC GT^[AC][GT]AC AccII 1291 CGCG CG^CG AcyI 2528 GGCGTC G[GA]^CG[CT]C AvaI 1820 CCCGGG C^[CT]CG[GA]G AvaII 2616 GGACC G^G[AT]CC BamHI 207 GGATCC G^GATCC BbvCI 2629 GCTGAGG CCTCAGC(-5/-2) BsaHI 2528 GGCGTC G[GA]^CG[CT]C BsaI 1662 GAGACC GGTCTC(1/5) BsaWI 743 TCCGGT [AT]^CCGG[AT] BsmBI 3001 GAGACG CGTCTC(1/5) BsmFI 1699 GTCCC GGGAC(10/14) BsoBI 1820 CCCGGG C^[CT]CG[GA]G Bsp1407I 2126 TGTACA T^GTACA BspLU11I 2162 ACATGT A^CATGT BsrDI 2074 CATTGC GCAATG(2/0) BsrGI 2126 TGTACA T^GTACA BstEII 2589 GGTGACC G^GT[ACGT]ACC BstPI 2589 GGTGACC G^GT[ACGT]ACC BstUI 1291 CGCG CG^CG BstXI 2861 CCAATAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 2097 GCGC GCG^C DraI 955 TTTAAA TTT^AAA DraII 2615 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 416 TGGCCG [CT]^GGCC[GA] EcoO109I 2615 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 2589 GGTGACC G^GT[ACGT]ACC FauI 2517 GCGGG CCCGC(4/6) HaeII 2096 AGCGCC [GA]GCGC^[CT] HhaI 2097 GCGC GCG^C Hin1I 2528 GGCGTC G[GA]^CG[CT]C HinP1I 2097 GCGC G^CGC HincII 2316 GTTGAC GT[CT]^[GA]AC HindII 2316 GTTGAC GT[CT]^[GA]AC HindIII 392 AAGCTT A^AGCTT MvnI 1291 CGCG CG^CG NdeI 1981 CATATG CA^TATG PpuMI 2615 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1281 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 1339 AGTACT AGT^ACT SexAI 1760 ACCTGGT A^CC[AT]GGT SmaI 1820 CCCGGG CCC^GGG SspBI 2126 TGTACA T^GTACA XmaCI 1820 CCCGGG C^CCGGG XmaI 1820 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflII 501 CTTAAG C^TTAAG 519 CTTAAG AflIII 735 ACACGT A^C[GA][CT]GT 2162 ACATGT BanI 2332 GGTGCC G^G[CT][GA]CC 2912 GGTGCC BciVI 773 GGATAC GTATCC(6/5) 1585 GTATCC BfrI 501 CTTAAG C^TTAAG 519 CTTAAG BsaBI 510 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 984 GATTGTCATC BsmI 1778 GCATTC GAATGC(1/-1) 1999 GCATTC BspMI 2237 ACCTGC ACCTGC(4/8) 2309 ACCTGC BstBI 2062 TTCGAA TT^CGAA 2298 TTCGAA HgaI 3003 GACGC GACGC(5/10) 2529 GCGTC MamI 510 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 984 GATTGTCATC NheI 189 GCTAGC G^CTAGC 1734 GCTAGC NspI 1082 ACATGC [GA]CATG^[CT] 2162 ACATGT NspV 2062 TTCGAA TT^CGAA 2298 TTCGAA SacI 721 GAGCTC GAGCT^C 3162 GAGCTC SapI 723 GCTCTTC GCTCTTC(1/4) 1708 GCTCTTC SfcI 1229 CTGTAG C^T[GA][CT]AG 1463 CTACAG SfuI 2062 TTCGAA TT^CGAA 2298 TTCGAA XcmI 482 CCAGGAGTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3050 CCAAACTCCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI