(Circular) (Six-base) MAPSORT of: pFN21AE4264.seq Check: 1796 from: 1 to: 7912 pFN21AE4264 7912 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5722 278 Size: 53 83 186 936 4186 2468 Fragments arranged by size: 4186 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4537 5255 1937 Size: 2600 718 4594 Fragments arranged by size: 4594 2600 718 Acc65I G'GTAC_C Cuts at: 5233 5233 Size: 7912 AclI AA'CG_TT Cuts at: 1563 6039 6412 1563 Size: 4476 373 3063 Fragments arranged by size: 4476 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2028 2415 2601 2716 3316 3465 3729 4014 Size: 396 387 186 115 600 149 264 285 Cuts at: 4014 5975 7359 7627 1632 Size: 1961 1384 268 1917 Fragments arranged by size: 1961 1917 1384 600 396 387 285 268 264 186 149 115 AflII C'TTAA_G Cuts at: 820 1017 2506 2524 820 Size: 197 1489 18 6208 Fragments arranged by size: 6208 1489 197 18 AflIII A'CryG_T Cuts at: 1203 2740 4167 6811 7788 1203 Size: 1537 1427 2644 977 1327 Fragments arranged by size: 2644 1537 1427 1327 977 AgeI A'CCGG_T Cuts at: 6743 6743 Size: 7912 AhdI GACnn_n'nnGTC Cuts at: 6641 6641 Size: 7912 AleI CACnn'nnGTG Cuts at: 1166 2417 1166 Size: 1251 6661 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7912 AlwNI CAG_nnn'CTG Cuts at: 2182 2840 4778 5655 7227 2182 Size: 658 1938 877 1572 2867 Fragments arranged by size: 2867 1938 1572 877 658 ApaLI G'TGCA_C Cuts at: 5969 7125 5969 Size: 1156 6756 ApoI r'AATT_y Cuts at: 1493 3355 3612 4085 4208 4887 4925 5221 5447 Size: 1862 257 473 123 679 38 296 226 Cuts at: 5447 5599 7708 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1862 1697 679 473 296 257 226 152 123 38 AseI AT'TA_AT Cuts at: 160 1032 6466 160 Size: 872 5434 1606 Fragments arranged by size: 5434 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7912 AvaI C'yCGr_G Cuts at: 1870 1943 3825 5237 1870 Size: 73 1882 1412 4545 Fragments arranged by size: 4545 1882 1412 73 BamHI G'GATC_C Cuts at: 2212 5242 5609 2212 Size: 3030 367 4515 Fragments arranged by size: 4515 3030 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4337 4917 5233 6688 618 Size: 325 217 627 2550 580 316 1455 1842 Fragments arranged by size: 2550 1842 1455 627 580 325 316 217 BanII G_rGCy'C Cuts at: 1391 2346 2730 4044 5171 5231 1391 Size: 955 384 1314 1127 60 4072 Fragments arranged by size: 4072 1314 1127 955 384 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2737 3041 4219 4506 5150 928 Size: 971 838 304 1178 287 644 3690 Fragments arranged by size: 3690 1178 971 838 644 304 287 BbvCI CC'TCA_GC Cuts at: 4635 4635 Size: 7912 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6103 6137 1903 Size: 34 4166 34 3678 Fragments arranged by size: 4166 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2772 3601 5804 7014 2772 Size: 829 2203 1210 3670 Fragments arranged by size: 3670 2203 1210 829 BclI T'GATC_A Cuts at: 1548 1548 Size: 7912 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6523 136 Size: 107 122 71 631 605 4851 1525 Fragments arranged by size: 4851 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7907 7907 Size: 7912 BlpI GC'TnA_GC Cuts at: 5329 5329 Size: 7912 Bme1580I G_kGCm'C Cuts at: 1790 5973 7129 1790 Size: 4183 1156 2573 Fragments arranged by size: 4183 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2743 5148 1318 Size: 1425 2405 4082 Fragments arranged by size: 4082 2405 1425 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4174 5643 6601 Size: 416 53 83 399 24 2757 1469 958 Cuts at: 6601 442 Size: 1753 Fragments arranged by size: 2757 1753 1469 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2198 3743 1056 Size: 1142 1545 5225 Fragments arranged by size: 5225 1545 1142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3302 3516 5045 6572 1239 Size: 2063 214 1529 1527 2579 Fragments arranged by size: 2579 2063 1529 1527 214 Bpu10I CC'TnA_GC Cuts at: 4043 4635 4043 Size: 592 7320 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 4997 6037 6902 7200 2135 Size: 2862 1040 865 298 2847 Fragments arranged by size: 2862 2847 1040 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3661 6575 882 Size: 617 2162 2914 2219 Fragments arranged by size: 2914 2219 2162 617 BsaAI yAC'GTr Cuts at: 493 1204 5703 493 Size: 711 4499 2702 Fragments arranged by size: 4499 2702 711 BsaBI GATnn'nnATC Cuts at: 2519 2993 7906 2519 Size: 474 4913 2525 Fragments arranged by size: 4913 2525 474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4534 5719 6101 6736 Size: 53 83 186 936 3001 1185 382 635 Cuts at: 6736 275 Size: 1451 Fragments arranged by size: 3001 1451 1185 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2748 6345 6743 7017 7164 7575 2748 Size: 3597 398 274 147 411 3085 Fragments arranged by size: 3597 3085 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5063 5093 1178 Size: 30 165 30 3660 30 3997 Fragments arranged by size: 3997 3660 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3472 3519 3735 3891 1186 Size: 2286 47 216 156 5207 Fragments arranged by size: 5207 2286 216 156 47 BseYI C'CCAG_C Cuts at: 3847 4491 7115 7611 3847 Size: 644 2624 496 4148 Fragments arranged by size: 4148 2624 644 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2829 2949 3023 3624 5150 1163 Size: 573 1093 120 74 601 1526 3925 Fragments arranged by size: 3925 1526 1093 601 573 120 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5362 6123 7151 1540 Size: 275 186 3361 761 1028 2301 Fragments arranged by size: 3361 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2730 3978 5171 5973 6058 7129 2730 Size: 1248 1193 802 85 1071 3513 Fragments arranged by size: 3513 1248 1193 1071 802 85 BsmI GAATG_Cn' Cuts at: 3783 4004 5423 5516 3783 Size: 221 1419 93 6179 Fragments arranged by size: 6179 1419 221 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5000 5000 Size: 7912 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2730 3978 4044 5171 5231 5973 Size: 399 556 384 1248 66 1127 60 742 Cuts at: 5973 6058 7129 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1248 1127 1071 742 556 399 384 85 66 60 BspEI T'CCGG_A Cuts at: 7575 7575 Size: 7912 BspHI T'CATG_A Cuts at: 5801 7531 5801 Size: 1730 6182 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4251 4323 5267 844 Size: 3407 72 944 3489 Fragments arranged by size: 3489 3407 944 72 BsrBI CCG'CTC Cuts at: 1394 1823 4380 4522 4915 5799 1394 Size: 429 2557 142 393 884 3507 Fragments arranged by size: 3507 2557 884 429 393 142 BsrDI GCAATG_nn' Cuts at: 65 1248 4078 6407 6581 65 Size: 1183 2830 2329 174 1396 Fragments arranged by size: 2830 2329 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6556 6743 1700 Size: 110 4746 187 2869 Fragments arranged by size: 4746 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4131 96 Size: 4035 3877 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7912 BssSI C'ACGA_G Cuts at: 5972 6984 5972 Size: 1012 6900 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7912 BstBI TT'CG_AA Cuts at: 4068 4304 6716 4068 Size: 236 2412 5264 Fragments arranged by size: 5264 2412 236 BstEII G'GTnAC_C Cuts at: 1184 4594 1184 Size: 3410 4502 BstXI CCAn_nnnn'nTGG Cuts at: 4873 4873 Size: 7912 BstYI r'GATC_y Cuts at: 1972 2212 2602 3578 5242 5609 5994 6011 7463 Size: 240 390 976 1664 367 385 17 1452 Cuts at: 7463 7549 7561 7907 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1664 1452 976 390 385 367 346 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6421 Fragments arranged by size: 6421 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7173 Fragments arranged by size: 7173 683 56 BtsI GCAGTG_nn' Cuts at: 2796 2838 4200 5428 6222 6242 2796 Size: 42 1362 1228 794 20 4466 Fragments arranged by size: 4466 1362 1228 794 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7912 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7877 DraI TTT'AAA Cuts at: 2962 5216 2962 Size: 2254 5658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6919 809 Size: 507 5603 1802 Fragments arranged by size: 5603 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2421 5359 6248 Size: 54 1425 50 275 18 591 2938 889 Cuts at: 6248 7877 8 Size: 1629 43 Fragments arranged by size: 2938 1629 1425 889 591 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5359 1537 Size: 275 3547 4090 Fragments arranged by size: 4090 3547 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2047 2657 2735 3540 3720 3927 4698 4719 Size: 694 610 78 805 180 207 771 21 Cuts at: 4719 4992 5842 1353 Size: 273 850 3423 Fragments arranged by size: 3423 850 805 771 694 610 273 207 180 78 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2795 6499 6873 7019 387 Size: 1064 138 1206 3704 374 146 1280 Fragments arranged by size: 3704 1280 1206 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2728 5169 2728 Size: 2441 5471 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2415 2601 2716 3302 3316 3465 Size: 393 396 387 186 115 586 14 149 Cuts at: 3465 3516 3729 4014 5045 5975 6572 7359 7627 Size: 51 213 285 1031 930 597 787 268 Cuts at: 7627 1239 Size: 1524 Fragments arranged by size: 1524 1031 930 787 597 586 396 393 387 285 268 213 186 149 115 51 14 EcoNI CCTnn'n_nnAGG Cuts at: 2382 4109 5123 2382 Size: 1727 1014 5171 Fragments arranged by size: 5171 1727 1014 EcoO109I rG'GnC_Cy Cuts at: 4621 4621 Size: 7912 EcoRI G'AATT_C Cuts at: 5221 5599 5221 Size: 378 7534 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4081 4081 Size: 7912 FspI TGC'GCA Cuts at: 6418 6418 Size: 7912 HaeII r_GCGC'y Cuts at: 1678 4105 7059 1678 Size: 2427 2954 2531 Fragments arranged by size: 2954 2531 2427 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2096 2128 2200 Size: 32 135 32 467 32 25 32 72 Cuts at: 2200 2232 3092 3119 3124 3145 3151 3177 3511 Size: 32 860 27 5 21 6 26 334 Cuts at: 3511 3543 3711 3743 4813 4845 6553 6585 6627 Size: 32 168 32 1070 32 1708 32 42 Cuts at: 6627 6659 1373 Size: 32 2626 Fragments arranged by size: 2626 1708 1070 860 467 334 168 135 72 42 32 32 32 32 32 32 32 32 32 32 27 26 25 21 6 5 HincII GTy'rAC Cuts at: 669 1657 1938 4323 5256 5502 669 Size: 988 281 2385 933 246 3079 Fragments arranged by size: 3079 2385 988 933 281 246 HindIII A'AGCT_T Cuts at: 748 2397 748 Size: 1649 6263 HpaI GTT'AAC Cuts at: 5502 5502 Size: 7912 KpnI G_GTAC'C Cuts at: 5237 5237 Size: 7912 MfeI C'AATT_G Cuts at: 5511 5511 Size: 7912 MluI A'CGCG_T Cuts at: 7788 7788 Size: 7912 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2005 4077 4539 7026 7210 1305 Size: 700 2072 462 2487 184 2007 Fragments arranged by size: 2487 2072 2007 700 462 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6090 Fragments arranged by size: 6090 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 2718 2999 3756 4170 5870 6229 Size: 648 1251 301 281 757 414 1700 359 Cuts at: 6229 6388 518 Size: 159 2042 Fragments arranged by size: 2042 1700 1251 757 648 414 359 301 281 159 MspA1I CmG'CkG Cuts at: 1606 2182 3233 3736 5023 5065 5328 6005 7153 Size: 576 1051 503 1287 42 263 677 1148 Cuts at: 7153 7398 1606 Size: 245 2120 Fragments arranged by size: 2120 1287 1148 1051 677 576 503 263 245 42 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6421 Fragments arranged by size: 6421 939 552 NdeI CA'TA_TG Cuts at: 387 3987 387 Size: 3600 4312 NheI G'CTAG_C Cuts at: 1052 2194 3739 1052 Size: 1142 1545 5225 Fragments arranged by size: 5225 1545 1142 NotI GC'GGCC_GC Cuts at: 5359 5359 Size: 7912 NspI r_CATG'y Cuts at: 1132 1229 3091 4171 5270 6815 1132 Size: 97 1862 1080 1099 1545 2229 Fragments arranged by size: 2229 1862 1545 1099 1080 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7912 PciI A'CATG_T Cuts at: 4167 6811 4167 Size: 2644 5268 PflMI CCAn_nnn'nTGG Cuts at: 1108 3500 4144 4591 5068 1108 Size: 2392 644 447 477 3952 Fragments arranged by size: 3952 2392 644 477 447 PfoI T'CCnGG_A Cuts at: 3061 3279 4143 3061 Size: 218 864 6830 Fragments arranged by size: 6830 864 218 PmeI GTTT'AAAC Cuts at: 5216 5216 Size: 7912 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5981 6013 7494 7526 5981 Size: 32 1481 32 6367 Fragments arranged by size: 6367 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4621 4621 Size: 7912 PshAI GACnn'nnGTC Cuts at: 1613 3290 1613 Size: 1677 6235 PsiI TTA'TAA Cuts at: 5482 5482 Size: 7912 PstI C_TGCA'G Cuts at: 830 5264 830 Size: 4434 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7912 PvuII CAG'CTG Cuts at: 2182 3233 3736 5023 5065 2182 Size: 1051 503 1287 42 5029 Fragments arranged by size: 5029 1287 1051 503 42 SacI G_AGCT'C Cuts at: 2730 5171 2730 Size: 2441 5471 SalI G'TCGA_C Cuts at: 1936 5254 1936 Size: 3318 4594 SapI GCTCTTCn'nnn_ Cuts at: 2735 3720 2735 Size: 985 6927 SbfI CC_TGCA'GG Cuts at: 5264 5264 Size: 7912 ScaI AGT'ACT Cuts at: 3346 6160 7872 3346 Size: 2814 1712 3386 Fragments arranged by size: 3386 2814 1712 SexAI A'CCwGG_T Cuts at: 3765 3765 Size: 7912 SfcI C'TryA_G Cuts at: 826 1045 3234 3468 5260 6395 7076 7267 826 Size: 219 2189 234 1792 1135 681 191 1471 Fragments arranged by size: 2189 1792 1471 1135 681 234 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7912 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7912 SmaI CCC'GGG Cuts at: 1872 3827 5239 1872 Size: 1955 1412 4545 Fragments arranged by size: 4545 1955 1412 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2506 2524 5012 6016 6917 Size: 197 926 207 356 18 2488 1004 901 Cuts at: 6917 7179 820 Size: 262 1553 Fragments arranged by size: 2488 1553 1004 926 901 356 262 207 197 18 SnaBI TAC'GTA Cuts at: 493 5703 493 Size: 5210 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7912 SphI G_CATG'C Cuts at: 1132 5270 1132 Size: 4138 3774 SspI AAT'ATT Cuts at: 5 52 5836 5 Size: 47 5784 2081 Fragments arranged by size: 5784 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7912 StyI C'CwwG_G Cuts at: 513 1065 2004 2971 4285 4968 5351 513 Size: 552 939 967 1314 683 383 3074 Fragments arranged by size: 3074 1314 967 939 683 552 383 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3685 5939 5956 6109 6294 6739 325 Size: 1255 2105 2254 17 153 185 445 1498 Fragments arranged by size: 2254 2105 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2203 3256 3344 Size: 274 80 33 51 1443 226 1053 88 Cuts at: 3344 4131 6158 7870 96 Size: 787 2027 1712 138 Fragments arranged by size: 2027 1712 1443 1053 787 274 226 138 88 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2155 2187 2939 2971 5063 5095 7872 7904 2155 Size: 32 752 32 2092 32 2777 32 2163 Fragments arranged by size: 2777 2163 2092 752 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7912 XbaI T'CTAG_A Cuts at: 5248 5248 Size: 7912 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 5062 2494 Size: 2568 5344 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7912 XmaI C'CCGG_G Cuts at: 1870 3825 5237 1870 Size: 1955 1412 4545 Fragments arranged by size: 4545 1955 1412 XmnI GAAnn'nnTTC Cuts at: 3612 3714 4119 6041 3612 Size: 102 405 1922 5483 Fragments arranged by size: 5483 1922 405 102 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5720 276 Size: 53 83 186 936 4186 2468 Fragments arranged by size: 4186 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI