(Linear) (Six-base) MAPSORT of: KIEE4264.seq Check: 1636 from: 1 to: 3207 KIEE4264.orf With 182 enzymes: SgfI * July 3, 2012 18:53 .. AccI GT'mk_AC Cuts at: 0 2532 3207 Size: 2532 675 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 410 596 711 1311 1460 1724 2009 Size: 23 387 186 115 600 149 264 285 Cuts at: 2009 3207 Size: 1198 Fragments arranged by size: 1198 600 387 285 264 186 149 115 23 AflII C'TTAA_G Cuts at: 0 501 519 3207 Size: 501 18 2688 Fragments arranged by size: 2688 501 18 AflIII A'CryG_T Cuts at: 0 735 2162 3207 Size: 735 1427 1045 Fragments arranged by size: 1427 1045 735 AleI CACnn'nnGTG Cuts at: 0 412 3207 Size: 412 2795 AlwNI CAG_nnn'CTG Cuts at: 0 177 835 2773 3207 Size: 177 658 1938 434 Fragments arranged by size: 1938 658 434 177 ApoI r'AATT_y Cuts at: 0 1350 1607 2080 2203 2882 2920 3207 Size: 1350 257 473 123 679 38 287 Fragments arranged by size: 1350 679 473 287 257 123 38 AvaI C'yCGr_G Cuts at: 0 1820 3207 Size: 1820 1387 BamHI G'GATC_C Cuts at: 0 207 3207 Size: 207 3000 BanI G'GyrC_C Cuts at: 0 2332 2912 3207 Size: 2332 580 295 Fragments arranged by size: 2332 580 295 BanII G_rGCy'C Cuts at: 0 341 725 2039 3166 3207 Size: 341 384 1314 1127 41 Fragments arranged by size: 1314 1127 384 341 41 BbsI GAAGACnn'nnnn_ Cuts at: 0 732 1036 2214 2501 3145 3207 Size: 732 304 1178 287 644 62 Fragments arranged by size: 1178 732 644 304 287 62 BbvCI CC'TCA_GC Cuts at: 0 2630 3207 Size: 2630 577 BciVI GTATCCnnnnn_n' Cuts at: 0 767 1596 3207 Size: 767 829 1611 Fragments arranged by size: 1611 829 767 BmgBI CAC'GTC Cuts at: 0 738 3143 3207 Size: 738 2405 64 Fragments arranged by size: 2405 738 64 BmrI ACTGGGnnnn_n' Cuts at: 0 2169 3207 Size: 2169 1038 BmtI G_CTAG'C Cuts at: 0 193 1738 3207 Size: 193 1545 1469 Fragments arranged by size: 1545 1469 193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1297 1511 3040 3207 Size: 1297 214 1529 167 Fragments arranged by size: 1529 1297 214 167 Bpu10I CC'TnA_GC Cuts at: 0 2038 2630 3207 Size: 2038 592 577 Fragments arranged by size: 2038 592 577 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 2992 3207 Size: 130 2862 215 Fragments arranged by size: 2862 215 130 BsaI GGTCTCn'nnnn_ Cuts at: 0 1656 3207 Size: 1656 1551 BsaBI GATnn'nnATC Cuts at: 0 514 988 3207 Size: 514 474 2219 Fragments arranged by size: 2219 514 474 BsaHI Gr'CG_yC Cuts at: 0 2529 3207 Size: 2529 678 BsaWI w'CCGG_w Cuts at: 0 743 3207 Size: 743 2464 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 3058 3088 3207 Size: 3058 30 119 Fragments arranged by size: 3058 119 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1467 1514 1730 1886 3207 Size: 1467 47 216 156 1321 Fragments arranged by size: 1467 1321 216 156 47 BseYI C'CCAG_C Cuts at: 0 1842 2486 3207 Size: 1842 644 721 Fragments arranged by size: 1842 721 644 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 824 944 1018 1619 3145 3207 Size: 824 120 74 601 1526 62 Fragments arranged by size: 1526 824 601 120 74 62 BsiHKAI G_wGCw'C Cuts at: 0 725 1973 3166 3207 Size: 725 1248 1193 41 Fragments arranged by size: 1248 1193 725 41 BsmI GAATG_Cn' Cuts at: 0 1778 1999 3207 Size: 1778 221 1208 Fragments arranged by size: 1778 1208 221 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2995 3207 Size: 2995 212 Bsp1286I G_dGCh'C Cuts at: 0 341 725 1973 2039 3166 3207 Size: 341 384 1248 66 1127 41 Fragments arranged by size: 1248 1127 384 341 66 41 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2246 2318 3207 Size: 2246 72 889 Fragments arranged by size: 2246 889 72 BsrBI CCG'CTC Cuts at: 0 2375 2517 2910 3207 Size: 2375 142 393 297 Fragments arranged by size: 2375 393 297 142 BsrDI GCAATG_nn' Cuts at: 0 2073 3207 Size: 2073 1134 BsrGI T'GTAC_A Cuts at: 0 2126 3207 Size: 2126 1081 BstBI TT'CG_AA Cuts at: 0 2063 2299 3207 Size: 2063 236 908 Fragments arranged by size: 2063 908 236 BstEII G'GTnAC_C Cuts at: 0 2589 3207 Size: 2589 618 BstXI CCAn_nnnn'nTGG Cuts at: 0 2868 3207 Size: 2868 339 BstYI r'GATC_y Cuts at: 0 207 597 1573 3207 Size: 207 390 976 1634 Fragments arranged by size: 1634 976 390 207 BtsI GCAGTG_nn' Cuts at: 0 791 833 2195 3207 Size: 791 42 1362 1012 Fragments arranged by size: 1362 1012 791 42 DraI TTT'AAA Cuts at: 0 957 3207 Size: 957 2250 EaeI y'GGCC_r Cuts at: 0 416 3207 Size: 416 2791 EarI CTCTTCn'nnn_ Cuts at: 0 42 652 730 1535 1715 1922 2693 2714 Size: 42 610 78 805 180 207 771 21 Cuts at: 2714 2987 3207 Size: 273 220 Fragments arranged by size: 805 771 610 273 220 207 180 78 42 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 790 3207 Size: 790 2417 EcoICRI GAG'CTC Cuts at: 0 723 3164 3207 Size: 723 2441 43 Fragments arranged by size: 2441 723 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 410 596 711 1297 1311 1460 1511 Size: 23 387 186 115 586 14 149 51 Cuts at: 1511 1724 2009 3040 3207 Size: 213 285 1031 167 Fragments arranged by size: 1031 586 387 285 213 186 167 149 115 51 23 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 377 2104 3118 3207 Size: 377 1727 1014 89 Fragments arranged by size: 1727 1014 377 89 EcoO109I rG'GnC_Cy Cuts at: 0 2616 3207 Size: 2616 591 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2076 3207 Size: 2076 1131 HaeII r_GCGC'y Cuts at: 0 2100 3207 Size: 2100 1107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 34 66 91 123 195 227 1087 1114 Size: 34 32 25 32 72 32 860 27 Cuts at: 1114 1119 1140 1146 1172 1506 1538 1706 1738 Size: 5 21 6 26 334 32 168 32 Cuts at: 1738 2808 2840 3207 Size: 1070 32 367 Fragments arranged by size: 1070 860 367 334 168 72 34 32 32 32 32 32 32 27 26 25 21 6 5 HincII GTy'rAC Cuts at: 0 2318 3207 Size: 2318 889 HindIII A'AGCT_T Cuts at: 0 392 3207 Size: 392 2815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2072 2534 3207 Size: 2072 462 673 Fragments arranged by size: 2072 673 462 MslI CAynn'nnrTG Cuts at: 0 412 713 994 1751 2165 3207 Size: 412 301 281 757 414 1042 Fragments arranged by size: 1042 757 414 412 301 281 MspA1I CmG'CkG Cuts at: 0 177 1228 1731 3018 3060 3207 Size: 177 1051 503 1287 42 147 Fragments arranged by size: 1287 1051 503 177 147 42 NdeI CA'TA_TG Cuts at: 0 1982 3207 Size: 1982 1225 NheI G'CTAG_C Cuts at: 0 189 1734 3207 Size: 189 1545 1473 Fragments arranged by size: 1545 1473 189 NspI r_CATG'y Cuts at: 0 1086 2166 3207 Size: 1086 1080 1041 Fragments arranged by size: 1086 1080 1041 PciI A'CATG_T Cuts at: 0 2162 3207 Size: 2162 1045 PflMI CCAn_nnn'nTGG Cuts at: 0 1495 2139 2586 3063 3207 Size: 1495 644 447 477 144 Fragments arranged by size: 1495 644 477 447 144 PfoI T'CCnGG_A Cuts at: 0 1056 1274 2138 3207 Size: 1056 218 864 1069 Fragments arranged by size: 1069 1056 864 218 PpuMI rG'GwC_Cy Cuts at: 0 2616 3207 Size: 2616 591 PshAI GACnn'nnGTC Cuts at: 0 1285 3207 Size: 1285 1922 PvuII CAG'CTG Cuts at: 0 177 1228 1731 3018 3060 3207 Size: 177 1051 503 1287 42 147 Fragments arranged by size: 1287 1051 503 177 147 42 SacI G_AGCT'C Cuts at: 0 725 3166 3207 Size: 725 2441 41 Fragments arranged by size: 2441 725 41 SapI GCTCTTCn'nnn_ Cuts at: 0 730 1715 3207 Size: 730 985 1492 Fragments arranged by size: 1492 985 730 ScaI AGT'ACT Cuts at: 0 1341 3207 Size: 1341 1866 SexAI A'CCwGG_T Cuts at: 0 1760 3207 Size: 1760 1447 SfcI C'TryA_G Cuts at: 0 1229 1463 3207 Size: 1229 234 1744 Fragments arranged by size: 1744 1229 234 SmaI CCC'GGG Cuts at: 0 1822 3207 Size: 1822 1385 SmlI C'TyrA_G Cuts at: 0 145 501 519 3007 3207 Size: 145 356 18 2488 200 Fragments arranged by size: 2488 356 200 145 18 StyI C'CwwG_G Cuts at: 0 966 2280 2963 3207 Size: 966 1314 683 244 Fragments arranged by size: 1314 966 683 244 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1680 3207 Size: 1680 1527 TatI w'GTAC_w Cuts at: 0 198 1251 1339 2126 3207 Size: 198 1053 88 787 1081 Fragments arranged by size: 1081 1053 787 198 88 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 150 182 934 966 3058 3090 3207 Size: 150 32 752 32 2092 32 117 Fragments arranged by size: 2092 752 150 117 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 489 3057 3207 Size: 489 2568 150 Fragments arranged by size: 2568 489 150 XmaI C'CCGG_G Cuts at: 0 1820 3207 Size: 1820 1387 XmnI GAAnn'nnTTC Cuts at: 0 1607 1709 2114 3207 Size: 1607 102 405 1093 Fragments arranged by size: 1607 1093 405 102 Enzymes that do cut: AccI AcuI AflII AflIII AleI AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BstBI BstEII BstXI BstYI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII MmeI MslI MspA1I NdeI NheI NspI PciI PflMI PfoI PpuMI PshAI PvuII SacI SapI ScaI SexAI SfcI SmaI SmlI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BplI BsaAI BsiEI BsiWI BspEI BspHI BsrFI BssHII BssSI BstAPI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI ZraI