################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AcyI AflII AflIII AlwNI AocI ApaI AscI Asp718I AspI AviII AvrII BbeI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraIII EagI EclXI Eco52I Eco81I EcoO65I EcoRI FauI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PmeI PshAI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 1631 TCCGGA T^CCGGA AclI 2765 AACGTT AA^CGTT AgeI 2560 ACCGGT A^CCGGT Aor51HI 1557 AGCGCT AGC^GCT AseI 2055 ATTAAT AT^TAAT AsnI 2055 ATTAAT AT^TAAT BciVI 896 GTATCC GTATCC(6/5) BclI 2369 TGATCA T^GATCA BseAI 1631 TCCGGA T^CCGGA BspEI 1631 TCCGGA T^CCGGA BstXI 1113 CCAAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 1490 GCGTGC GC[ACGT]^[ACGT]GC DraI 2407 TTTAAA TTT^AAA DrdI 1952 GACCTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1557 AGCGCT AGC^GCT EcoNI 633 CCTAGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1528 GATATC GAT^ATC EcoT22I 2121 ATGCAT ATGCA^T FbaI 2369 TGATCA T^GATCA HgaI 28 GACGC GACGC(5/10) MroI 1631 TCCGGA T^CCGGA MslI 2557 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 544 CAGCTG C[AC]G^C[GT]G NsiI 2121 ATGCAT ATGCA^T NspI 1232 ACATGC [GA]CATG^[CT] PinAI 2560 ACCGGT A^CCGGT PshBI 2055 ATTAAT AT^TAAT Psp1406I 2765 AACGTT AA^CGTT PvuII 544 CAGCTG CAG^CTG SacI 2526 GAGCTC GAGCT^C SapI 1578 GAAGAGC GCTCTTC(1/4) SgrAI 2559 CACCGGTG C[GA]^CCGG[CT]G SmlI 253 CTTGAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 816 GTCTAC GT^[AC][GT]AC 1961 GTCTAC AccII 30 CGCG CG^CG 1719 CGCG AfaI 2090 GTAC GT^AC 2696 GTAC AhdI 392 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 864 GACTCTAGGTC Alw44I 412 GTGCAC G^TGCAC 1492 GTGCAC ApaLI 412 GTGCAC G^TGCAC 1492 GTGCAC AspEI 392 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 864 GACTCTAGGTC BamHI 1842 GGATCC G^GATCC 2010 GGATCC BanI 1459 GGTGCC G^G[CT][GA]CC 2455 GGTGCC BbrPI 697 CACGTG CAC^GTG 1702 CACGTG BsaAI 697 CACGTG [CT]AC^GT[GA] 1702 CACGTG BsgI 809 GTGCAG GTGCAG(16/14) 959 GTGCAG BsrFI 212 GCCGGT [GA]^CCGG[CT] 2560 ACCGGT BstAPI 952 GCAAGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1285 GCAGCCCGTGC BstUI 30 CGCG CG^CG 1719 CGCG BstYI 1842 GGATCC [GA]^GATC[CT] 2010 GGATCC Cfr10I 212 GCCGGT [GA]^CCGG[CT] 2560 ACCGGT DsaI 859 CCATGG C^C[GA][CT]GG 1455 CCATGG Eam1105I 392 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 864 GACTCTAGGTC HaeII 1557 AGCGCT [GA]GCGC^[CT] 2623 AGCGCC MflI 1842 GGATCC [GA]^GATC[CT] 2010 GGATCC MvnI 30 CGCG CG^CG 1719 CGCG NcoI 859 CCATGG C^CATGG 1455 CCATGG PmaCI 697 CACGTG CAC^GTG 1702 CACGTG PmlI 697 CACGTG CAC^GTG 1702 CACGTG RsaI 2090 GTAC GT^AC 2696 GTAC StuI 1094 AGGCCT AGG^CCT 2218 AGGCCT Tsp45I 166 GTGAC ^GT[GC]AC 646 GTGAC XhoII 1842 GGATCC [GA]^GATC[CT] 2010 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI