(Circular) (Six-base) MAPSORT of: pFN21AE3333.seq Check: 645 from: 1 to: 7606 pFN21AE3333 7606 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2470 3011 2470 Size: 541 7065 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5416 278 Size: 53 83 186 936 3880 2468 Fragments arranged by size: 3880 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2822 3967 4949 1937 Size: 885 1145 982 4594 Fragments arranged by size: 4594 1145 982 885 Acc65I G'GTAC_C Cuts at: 4927 4927 Size: 7606 AclI AA'CG_TT Cuts at: 1563 4771 5733 6106 1563 Size: 3208 962 373 3063 Fragments arranged by size: 3208 3063 962 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2131 2246 3080 3365 3659 3888 5669 7053 Size: 499 115 834 285 294 229 1781 1384 Cuts at: 7053 7321 1632 Size: 268 1917 Fragments arranged by size: 1917 1781 1384 834 499 294 285 268 229 115 AfeI AGC'GCT Cuts at: 3564 3564 Size: 7606 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7409 AflIII A'CryG_T Cuts at: 1203 6505 7482 1203 Size: 5302 977 1327 Fragments arranged by size: 5302 1327 977 AgeI A'CCGG_T Cuts at: 4565 6437 4565 Size: 1872 5734 AhdI GACnn_n'nnGTC Cuts at: 2402 2874 6335 2402 Size: 472 3461 3673 Fragments arranged by size: 3673 3461 472 AleI CACnn'nnGTG Cuts at: 1166 4566 1166 Size: 3400 4206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2432 1184 Size: 1248 6358 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2786 2818 2786 Size: 32 7574 AlwNI CAG_nnn'CTG Cuts at: 5349 6921 5349 Size: 1572 6034 ApaLI G'TGCA_C Cuts at: 2417 3497 5663 6819 2417 Size: 1080 2166 1156 3204 Fragments arranged by size: 3204 2166 1156 1080 ApoI r'AATT_y Cuts at: 1493 3437 4453 4889 4915 5141 5293 7402 1493 Size: 1944 1016 436 26 226 152 2109 1697 Fragments arranged by size: 2109 1944 1697 1016 436 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 4061 6160 160 Size: 872 3029 2099 1606 Fragments arranged by size: 3029 2099 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7606 AvaI C'yCGr_G Cuts at: 1870 1943 2297 2508 3759 4931 1870 Size: 73 354 211 1251 1172 4545 Fragments arranged by size: 4545 1251 1172 354 211 73 BamHI G'GATC_C Cuts at: 3847 4015 4936 5303 3847 Size: 168 921 367 6150 Fragments arranged by size: 6150 921 367 168 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3464 4460 4927 6382 618 Size: 325 217 627 1677 996 467 1455 1842 Fragments arranged by size: 1842 1677 1455 996 627 467 325 217 BanII G_rGCy'C Cuts at: 1391 2103 3980 4535 4925 1391 Size: 712 1877 555 390 4072 Fragments arranged by size: 4072 1877 712 555 390 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2395 3903 4144 928 Size: 971 496 1508 241 4390 Fragments arranged by size: 4390 1508 971 496 241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5797 5831 1903 Size: 34 3860 34 3678 Fragments arranged by size: 3860 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2912 5498 6708 2912 Size: 2586 1210 3810 Fragments arranged by size: 3810 2586 1210 BclI T'GATC_A Cuts at: 1548 4374 1548 Size: 2826 4780 BfrBI ATG'CAT Cuts at: 4128 4128 Size: 7606 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6217 136 Size: 107 122 71 631 605 4545 1525 Fragments arranged by size: 4545 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7601 7601 Size: 7606 BlpI GC'TnA_GC Cuts at: 5023 5023 Size: 7606 Bme1580I G_kGCm'C Cuts at: 1790 2320 2421 3469 3501 4465 4759 5667 6823 Size: 530 101 1048 32 964 294 908 1156 Cuts at: 6823 1790 Size: 2573 Fragments arranged by size: 2573 1156 1048 964 908 530 294 101 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3511 4659 4753 Size: 416 53 83 399 24 2094 1148 94 Cuts at: 4753 5337 6295 442 Size: 584 958 1753 Fragments arranged by size: 2094 1753 1148 958 584 416 399 94 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7606 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 7606 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 3188 3945 4755 6266 1239 Size: 824 1125 757 810 1511 2579 Fragments arranged by size: 2579 1511 1125 824 810 757 Bpu10I CC'TnA_GC Cuts at: 3366 3366 Size: 7606 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2279 5731 6596 6894 2279 Size: 3452 865 298 2991 Fragments arranged by size: 3452 2991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2123 2480 2806 3866 4234 6269 882 Size: 617 624 357 326 1060 368 2035 2219 Fragments arranged by size: 2219 2035 1060 624 617 368 357 326 BsaAI yAC'GTr Cuts at: 493 1204 2704 3709 5397 493 Size: 711 1500 1005 1688 2702 Fragments arranged by size: 2702 1688 1500 1005 711 BsaBI GATnn'nnATC Cuts at: 7600 7600 Size: 7606 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5413 5795 6430 275 Size: 53 83 186 936 3880 382 635 1451 Fragments arranged by size: 3880 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3636 4565 4635 6039 6437 6711 6858 7269 3636 Size: 929 70 1404 398 274 147 411 3973 Fragments arranged by size: 3973 1404 929 411 398 274 147 70 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2786 2816 4273 4303 1178 Size: 30 165 30 1383 30 1457 30 4481 Fragments arranged by size: 4481 1457 1383 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3404 3407 3938 4438 1186 Size: 2218 3 531 500 4354 Fragments arranged by size: 4354 2218 531 500 3 BseYI C'CCAG_C Cuts at: 2672 3779 3858 4103 6809 7305 2672 Size: 1107 79 245 2706 496 2973 Fragments arranged by size: 2973 2706 1107 496 245 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2835 2985 1163 Size: 573 1099 150 5784 Fragments arranged by size: 5784 1099 573 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5056 5817 6845 1540 Size: 275 186 3055 761 1028 2301 Fragments arranged by size: 3055 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2421 3301 3501 4286 4535 5667 5752 6823 2421 Size: 880 200 785 249 1132 85 1071 3204 Fragments arranged by size: 3204 1132 1071 880 785 249 200 85 BsmI GAATG_Cn' Cuts at: 2593 2881 4130 5117 5210 2593 Size: 288 1249 987 93 4989 Fragments arranged by size: 4989 1249 987 288 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2320 2421 3301 3469 3501 3980 Size: 399 313 217 101 880 168 32 479 Cuts at: 3980 4286 4465 4535 4759 4925 5667 5752 6823 Size: 306 179 70 224 166 742 85 1071 Cuts at: 6823 1391 Size: 2174 Fragments arranged by size: 2174 1071 880 742 479 399 313 306 224 217 179 168 166 101 85 70 32 BspEI T'CCGG_A Cuts at: 3636 7269 3636 Size: 3633 3973 BspHI T'CATG_A Cuts at: 5495 7225 5495 Size: 1730 5876 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2392 2470 3011 4961 844 Size: 1548 78 541 1950 3489 Fragments arranged by size: 3489 1950 1548 541 78 BsrBI CCG'CTC Cuts at: 1394 1823 5493 1394 Size: 429 3670 3507 Fragments arranged by size: 3670 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6101 6275 65 Size: 1183 4853 174 1396 Fragments arranged by size: 4853 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 4565 6250 6437 1700 Size: 110 407 2348 1685 187 2869 Fragments arranged by size: 2869 2348 1685 407 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7606 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7606 BssSI C'ACGA_G Cuts at: 5666 6678 5666 Size: 1012 6594 BstAPI GCAn_nnn'nTGC Cuts at: 794 2963 3296 794 Size: 2169 333 5104 Fragments arranged by size: 5104 2169 333 BstBI TT'CG_AA Cuts at: 6410 6410 Size: 7606 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7606 BstXI CCAn_nnnn'nTGG Cuts at: 3125 3125 Size: 7606 BstYI r'GATC_y Cuts at: 1972 3847 4015 4936 5303 5688 5705 7157 7243 Size: 1875 168 921 367 385 17 1452 86 Cuts at: 7243 7255 7601 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1875 1452 921 385 367 346 168 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2864 3460 513 Size: 552 939 860 596 4659 Fragments arranged by size: 4659 939 860 596 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6867 Fragments arranged by size: 6867 683 56 BtsI GCAGTG_nn' Cuts at: 2333 2823 2999 3913 5122 5916 5936 2333 Size: 490 176 914 1209 794 20 4003 Fragments arranged by size: 4003 1209 914 794 490 176 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7606 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7571 DraI TTT'AAA Cuts at: 4414 4910 4414 Size: 496 7110 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3963 6613 809 Size: 507 2647 2650 1802 Fragments arranged by size: 2650 2647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2706 3232 3703 Size: 54 1425 50 275 18 876 526 471 Cuts at: 3703 5053 5942 7571 8 Size: 1350 889 1629 43 Fragments arranged by size: 1629 1425 1350 889 876 526 471 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5053 1537 Size: 275 3241 4090 Fragments arranged by size: 4090 3241 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3341 3578 3674 3864 4232 5536 1353 Size: 1988 237 96 190 368 1304 3423 Fragments arranged by size: 3423 1988 1304 368 237 190 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6193 6567 6713 387 Size: 1064 138 4604 374 146 1280 Fragments arranged by size: 4604 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4533 4533 Size: 7606 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2131 2246 3080 3188 3365 3659 Size: 393 431 68 115 834 108 177 294 Cuts at: 3659 3888 3945 4755 5669 6266 7053 7321 1239 Size: 229 57 810 914 597 787 268 1524 Fragments arranged by size: 1524 914 834 810 787 597 431 393 294 268 229 177 115 108 68 57 EcoNI CCTnn'n_nnAGG Cuts at: 2642 2642 Size: 7606 EcoO109I rG'GnC_Cy Cuts at: 2080 2676 2758 2950 3956 2080 Size: 596 82 192 1006 5730 Fragments arranged by size: 5730 1006 596 192 82 EcoRI G'AATT_C Cuts at: 4915 5293 4915 Size: 378 7228 EcoRV GAT'ATC Cuts at: 3535 3535 Size: 7606 FspI TGC'GCA Cuts at: 6112 6112 Size: 7606 HaeII r_GCGC'y Cuts at: 1678 3566 4632 6753 1678 Size: 1888 1066 2121 2531 Fragments arranged by size: 2531 2121 1888 1066 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2388 2420 2722 Size: 32 135 32 506 32 278 32 302 Cuts at: 2722 2754 2860 2892 3742 3774 4273 4305 4522 Size: 32 106 32 850 32 499 32 217 Cuts at: 4522 4554 6247 6279 6321 6353 1373 Size: 32 1693 32 42 32 2626 Fragments arranged by size: 2626 1693 850 506 499 302 278 217 135 106 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4950 5196 669 Size: 988 281 3012 246 3079 Fragments arranged by size: 3079 3012 988 281 246 HindIII A'AGCT_T Cuts at: 748 2187 4270 4435 748 Size: 1439 2083 165 3919 Fragments arranged by size: 3919 2083 1439 165 HpaI GTT'AAC Cuts at: 5196 5196 Size: 7606 KpnI G_GTAC'C Cuts at: 4931 4931 Size: 7606 MfeI C'AATT_G Cuts at: 5205 5205 Size: 7606 MluI A'CGCG_T Cuts at: 7482 7482 Size: 7606 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2826 4043 6720 6904 1305 Size: 1521 1217 2677 184 2007 Fragments arranged by size: 2677 2007 1521 1217 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2708 3234 3705 10 Size: 54 1425 343 876 526 471 3911 Fragments arranged by size: 3911 1425 876 526 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4566 5564 5923 6082 518 Size: 648 3400 998 359 159 2042 Fragments arranged by size: 3400 2042 998 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2551 5022 5699 6847 7092 1606 Size: 945 2471 677 1148 245 2120 Fragments arranged by size: 2471 2120 1148 945 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2864 3460 513 Size: 552 939 860 596 4659 Fragments arranged by size: 4659 939 860 596 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7606 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7606 NotI GC'GGCC_GC Cuts at: 5053 5053 Size: 7606 NsiI A_TGCA'T Cuts at: 4130 4130 Size: 7606 NspI r_CATG'y Cuts at: 1132 1229 3241 4964 6509 1132 Size: 97 2012 1723 1545 2229 Fragments arranged by size: 2229 2012 1723 1545 97 PasI CC'CwG_GG Cuts at: 1348 2496 2604 2806 1348 Size: 1148 108 202 6148 Fragments arranged by size: 6148 1148 202 108 PciI A'CATG_T Cuts at: 6505 6505 Size: 7606 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7606 PmeI GTTT'AAAC Cuts at: 4910 4910 Size: 7606 PmlI CAC'GTG Cuts at: 2704 3709 2704 Size: 1005 6601 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2786 2818 3932 3964 5675 5707 7188 7220 2786 Size: 32 1114 32 1711 32 1481 32 3172 Fragments arranged by size: 3172 1711 1481 1114 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2676 2758 2950 3956 2080 Size: 596 82 192 1006 5730 Fragments arranged by size: 5730 1006 596 192 82 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7606 PsiI TTA'TAA Cuts at: 5176 5176 Size: 7606 PstI C_TGCA'G Cuts at: 830 3292 3406 3975 4958 830 Size: 2462 114 569 983 3478 Fragments arranged by size: 3478 2462 983 569 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7606 PvuII CAG'CTG Cuts at: 2551 2551 Size: 7606 SacI G_AGCT'C Cuts at: 4535 4535 Size: 7606 SalI G'TCGA_C Cuts at: 1936 4948 1936 Size: 3012 4594 SanDI GG'GwC_CC Cuts at: 2676 2676 Size: 7606 SapI GCTCTTCn'nnn_ Cuts at: 3578 3578 Size: 7606 SbfI CC_TGCA'GG Cuts at: 4958 4958 Size: 7606 ScaI AGT'ACT Cuts at: 5854 7566 5854 Size: 1712 5894 SfcI C'TryA_G Cuts at: 826 1045 2722 3288 3402 3603 3852 3971 4246 Size: 219 1677 566 114 201 249 119 275 Cuts at: 4246 4495 4954 6089 6770 6961 826 Size: 249 459 1135 681 191 1471 Fragments arranged by size: 1677 1471 1135 681 566 459 275 249 249 219 201 191 119 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7606 SgrAI Cr'CCGG_yG Cuts at: 1700 4565 1700 Size: 2865 4741 SmaI CCC'GGG Cuts at: 1872 4933 1872 Size: 3061 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 5710 6611 6873 820 Size: 197 926 315 3452 901 262 1553 Fragments arranged by size: 3452 1553 926 901 315 262 197 SnaBI TAC'GTA Cuts at: 493 5397 493 Size: 4904 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7606 SphI G_CATG'C Cuts at: 1132 4964 1132 Size: 3832 3774 SspI AAT'ATT Cuts at: 5 52 5530 5 Size: 47 5478 2081 Fragments arranged by size: 5478 2081 47 StuI AGG'CCT Cuts at: 1517 3101 4225 1517 Size: 1584 1124 4898 Fragments arranged by size: 4898 1584 1124 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 2864 3460 5045 513 Size: 552 939 240 620 596 1585 3074 Fragments arranged by size: 3074 1585 939 620 596 552 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5633 5650 5803 5988 6433 325 Size: 1255 4053 17 153 185 445 1498 Fragments arranged by size: 4053 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5852 7564 96 Size: 274 80 33 51 1443 3875 1712 138 Fragments arranged by size: 3875 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2072 2104 2650 2682 2802 2834 7566 7598 2072 Size: 32 546 32 120 32 4732 32 2080 Fragments arranged by size: 4732 2080 546 120 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7606 XbaI T'CTAG_A Cuts at: 4942 4942 Size: 7606 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7606 XmaI C'CCGG_G Cuts at: 1870 4931 1870 Size: 3061 4545 XmnI GAAnn'nnTTC Cuts at: 3093 3564 3615 4152 4604 5735 3093 Size: 471 51 537 452 1131 4964 Fragments arranged by size: 4964 1131 537 471 452 51 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5414 276 Size: 53 83 186 936 3880 2468 Fragments arranged by size: 3880 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI