(Linear) (Six-base) MAPSORT of: KIEE3333.seq Check: 5334 from: 1 to: 2901 KIEE3333.orf With 182 enzymes: SgfI * July 3, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 465 1006 2901 Size: 465 541 1895 Fragments arranged by size: 1895 541 465 AccI GT'mk_AC Cuts at: 0 817 1962 2901 Size: 817 1145 939 Fragments arranged by size: 1145 939 817 AclI AA'CG_TT Cuts at: 0 2766 2901 Size: 2766 135 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 126 241 1075 1360 1654 1883 2901 Size: 126 115 834 285 294 229 1018 Fragments arranged by size: 1018 834 294 285 229 126 115 AfeI AGC'GCT Cuts at: 0 1559 2901 Size: 1559 1342 AgeI A'CCGG_T Cuts at: 0 2560 2901 Size: 2560 341 AhdI GACnn_n'nnGTC Cuts at: 0 397 869 2901 Size: 397 472 2032 Fragments arranged by size: 2032 472 397 AleI CACnn'nnGTG Cuts at: 0 2561 2901 Size: 2561 340 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 427 2901 Size: 427 2474 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 781 813 2901 Size: 781 32 2088 Fragments arranged by size: 2088 781 32 ApaLI G'TGCA_C Cuts at: 0 412 1492 2901 Size: 412 1080 1409 Fragments arranged by size: 1409 1080 412 ApoI r'AATT_y Cuts at: 0 1432 2448 2884 2901 Size: 1432 1016 436 17 Fragments arranged by size: 1432 1016 436 17 AseI AT'TA_AT Cuts at: 0 2056 2901 Size: 2056 845 AvaI C'yCGr_G Cuts at: 0 292 503 1754 2901 Size: 292 211 1251 1147 Fragments arranged by size: 1251 1147 292 211 BamHI G'GATC_C Cuts at: 0 1842 2010 2901 Size: 1842 168 891 Fragments arranged by size: 1842 891 168 BanI G'GyrC_C Cuts at: 0 1459 2455 2901 Size: 1459 996 446 Fragments arranged by size: 1459 996 446 BanII G_rGCy'C Cuts at: 0 98 1975 2530 2901 Size: 98 1877 555 371 Fragments arranged by size: 1877 555 371 98 BbsI GAAGACnn'nnnn_ Cuts at: 0 390 1898 2139 2901 Size: 390 1508 241 762 Fragments arranged by size: 1508 762 390 241 BciVI GTATCCnnnnn_n' Cuts at: 0 907 2901 Size: 907 1994 BclI T'GATC_A Cuts at: 0 2369 2901 Size: 2369 532 BfrBI ATG'CAT Cuts at: 0 2123 2901 Size: 2123 778 Bme1580I G_kGCm'C Cuts at: 0 315 416 1464 1496 2460 2754 2901 Size: 315 101 1048 32 964 294 147 Fragments arranged by size: 1048 964 315 294 147 101 32 BmrI ACTGGGnnnn_n' Cuts at: 0 1506 2654 2748 2901 Size: 1506 1148 94 153 Fragments arranged by size: 1506 1148 153 94 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 444 2901 Size: 444 2457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 1183 1940 2750 2901 Size: 58 1125 757 810 151 Fragments arranged by size: 1125 810 757 151 58 Bpu10I CC'TnA_GC Cuts at: 0 1361 2901 Size: 1361 1540 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 2901 Size: 274 2627 BsaI GGTCTCn'nnnn_ Cuts at: 0 118 475 801 1861 2229 2901 Size: 118 357 326 1060 368 672 Fragments arranged by size: 1060 672 368 357 326 118 BsaAI yAC'GTr Cuts at: 0 699 1704 2901 Size: 699 1005 1197 Fragments arranged by size: 1197 1005 699 BsaWI w'CCGG_w Cuts at: 0 1631 2560 2630 2901 Size: 1631 929 70 271 Fragments arranged by size: 1631 929 271 70 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 781 811 2268 2298 2901 Size: 781 30 1457 30 603 Fragments arranged by size: 1457 781 603 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1399 1402 1933 2433 2901 Size: 1399 3 531 500 468 Fragments arranged by size: 1399 531 500 468 3 BseYI C'CCAG_C Cuts at: 0 667 1774 1853 2098 2901 Size: 667 1107 79 245 803 Fragments arranged by size: 1107 803 667 245 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 830 980 2901 Size: 830 150 1921 Fragments arranged by size: 1921 830 150 BsiHKAI G_wGCw'C Cuts at: 0 416 1296 1496 2281 2530 2901 Size: 416 880 200 785 249 371 Fragments arranged by size: 880 785 416 371 249 200 BsmI GAATG_Cn' Cuts at: 0 588 876 2125 2901 Size: 588 288 1249 776 Fragments arranged by size: 1249 776 588 288 Bsp1286I G_dGCh'C Cuts at: 0 98 315 416 1296 1464 1496 1975 2281 Size: 98 217 101 880 168 32 479 306 Cuts at: 2281 2460 2530 2754 2901 Size: 179 70 224 147 Fragments arranged by size: 880 479 306 224 217 179 168 147 101 98 70 32 BspEI T'CCGG_A Cuts at: 0 1631 2901 Size: 1631 1270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 387 465 1006 2901 Size: 387 78 541 1895 Fragments arranged by size: 1895 541 387 78 BsrFI r'CCGG_y Cuts at: 0 212 2560 2901 Size: 212 2348 341 Fragments arranged by size: 2348 341 212 BstAPI GCAn_nnn'nTGC Cuts at: 0 958 1291 2901 Size: 958 333 1610 Fragments arranged by size: 1610 958 333 BstXI CCAn_nnnn'nTGG Cuts at: 0 1120 2901 Size: 1120 1781 BstYI r'GATC_y Cuts at: 0 1842 2010 2901 Size: 1842 168 891 Fragments arranged by size: 1842 891 168 BtgI C'CryG_G Cuts at: 0 859 1455 2901 Size: 859 596 1446 Fragments arranged by size: 1446 859 596 BtsI GCAGTG_nn' Cuts at: 0 328 818 994 1908 2901 Size: 328 490 176 914 993 Fragments arranged by size: 993 914 490 328 176 DraI TTT'AAA Cuts at: 0 2409 2901 Size: 2409 492 DrdI GACnn_nn'nnGTC Cuts at: 0 1958 2901 Size: 1958 943 EaeI y'GGCC_r Cuts at: 0 701 1227 1698 2901 Size: 701 526 471 1203 Fragments arranged by size: 1203 701 526 471 EarI CTCTTCn'nnn_ Cuts at: 0 1336 1573 1669 1859 2227 2901 Size: 1336 237 96 190 368 674 Fragments arranged by size: 1336 674 368 237 190 96 EcoICRI GAG'CTC Cuts at: 0 2528 2901 Size: 2528 373 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 126 241 1075 1183 1360 1654 1883 Size: 58 68 115 834 108 177 294 229 Cuts at: 1883 1940 2750 2901 Size: 57 810 151 Fragments arranged by size: 834 810 294 229 177 151 115 108 68 58 57 EcoNI CCTnn'n_nnAGG Cuts at: 0 637 2901 Size: 637 2264 EcoO109I rG'GnC_Cy Cuts at: 0 75 671 753 945 1951 2901 Size: 75 596 82 192 1006 950 Fragments arranged by size: 1006 950 596 192 82 75 EcoRV GAT'ATC Cuts at: 0 1530 2901 Size: 1530 1371 HaeII r_GCGC'y Cuts at: 0 1561 2627 2901 Size: 1561 1066 274 Fragments arranged by size: 1561 1066 274 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 73 105 383 415 717 749 855 887 Size: 73 32 278 32 302 32 106 32 Cuts at: 887 1737 1769 2268 2300 2517 2549 2901 Size: 850 32 499 32 217 32 352 Fragments arranged by size: 850 499 352 302 278 217 106 73 32 32 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 182 2265 2430 2901 Size: 182 2083 165 471 Fragments arranged by size: 2083 471 182 165 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 821 2038 2901 Size: 821 1217 863 Fragments arranged by size: 1217 863 821 MscI TGG'CCA Cuts at: 0 703 1229 1700 2901 Size: 703 526 471 1201 Fragments arranged by size: 1201 703 526 471 MslI CAynn'nnrTG Cuts at: 0 2561 2901 Size: 2561 340 MspA1I CmG'CkG Cuts at: 0 546 2901 Size: 546 2355 NcoI C'CATG_G Cuts at: 0 859 1455 2901 Size: 859 596 1446 Fragments arranged by size: 1446 859 596 NsiI A_TGCA'T Cuts at: 0 2125 2901 Size: 2125 776 NspI r_CATG'y Cuts at: 0 1236 2901 Size: 1236 1665 PasI CC'CwG_GG Cuts at: 0 491 599 801 2901 Size: 491 108 202 2100 Fragments arranged by size: 2100 491 202 108 PmlI CAC'GTG Cuts at: 0 699 1704 2901 Size: 699 1005 1197 Fragments arranged by size: 1197 1005 699 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 781 813 1927 1959 2901 Size: 781 32 1114 32 942 Fragments arranged by size: 1114 942 781 32 32 PpuMI rG'GwC_Cy Cuts at: 0 75 671 753 945 1951 2901 Size: 75 596 82 192 1006 950 Fragments arranged by size: 1006 950 596 192 82 75 PstI C_TGCA'G Cuts at: 0 1287 1401 1970 2901 Size: 1287 114 569 931 Fragments arranged by size: 1287 931 569 114 PvuII CAG'CTG Cuts at: 0 546 2901 Size: 546 2355 SacI G_AGCT'C Cuts at: 0 2530 2901 Size: 2530 371 SanDI GG'GwC_CC Cuts at: 0 671 2901 Size: 671 2230 SapI GCTCTTCn'nnn_ Cuts at: 0 1573 2901 Size: 1573 1328 SfcI C'TryA_G Cuts at: 0 717 1283 1397 1598 1847 1966 2241 2490 Size: 717 566 114 201 249 119 275 249 Cuts at: 2490 2901 Size: 411 Fragments arranged by size: 717 566 411 275 249 249 201 119 114 SgrAI Cr'CCGG_yG Cuts at: 0 2560 2901 Size: 2560 341 SmlI C'TyrA_G Cuts at: 0 253 2901 Size: 253 2648 StuI AGG'CCT Cuts at: 0 1096 2220 2901 Size: 1096 1124 681 Fragments arranged by size: 1124 1096 681 StyI C'CwwG_G Cuts at: 0 239 859 1455 2901 Size: 239 620 596 1446 Fragments arranged by size: 1446 620 596 239 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 67 99 645 677 797 829 2901 Size: 67 32 546 32 120 32 2072 Fragments arranged by size: 2072 546 120 67 32 32 32 XmnI GAAnn'nnTTC Cuts at: 0 1088 1559 1610 2147 2599 2901 Size: 1088 471 51 537 452 302 Fragments arranged by size: 1088 537 471 452 302 51 Enzymes that do cut: AarI AccI AclI AcuI AfeI AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BciVI BclI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrFI BstAPI BstXI BstYI BtgI BtsI DraI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PmlI PpiI PpuMI PstI PvuII SacI SanDI SapI SfcI SgrAI SmlI StuI StyI TstI XmnI Enzymes that do not cut: AatII Acc65I AflII AflIII AlwNI ApaI AscI AsiSI AvrII BaeI BbeI BbvCI BcgI BglI BglII BlpI BmgBI BmtI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EagI EciI EcoRI FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PflMI PfoI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI ZraI