################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NarI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 445 GTCTAC GT^[AC][GT]AC AocI 88 CCTGAGG CC^T[ACGT]AGG Aor51HI 410 AGCGCT AGC^GCT BamHI 78 GGATCC G^GATCC BciVI 192 GGATAC GTATCC(6/5) BpmI 466 CTGGAG CTGGAG(16/14) BsiEI 24 CGGCCG CG[GA][CT]^CG BsmAI 254 GTCTC GTCTC(1/5) BsmBI 253 CGTCTC CGTCTC(1/5) BssSI 259 CACGAG CACGAG(-5/-1) BstXI 143 CCATCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 88 CCTGAGG CC^T[ACGT]AGG DdeI 89 CTGAG C^T[ACGT]AG EagI 24 CGGCCG C^GGCCG EarI 334 GAAGAG CTCTTC(1/4) EclXI 24 CGGCCG C^GGCCG Eco47III 410 AGCGCT AGC^GCT Eco52I 24 CGGCCG C^GGCCG Eco81I 88 CCTGAGG CC^T[ACGT]AGG HaeII 410 AGCGCT [GA]GCGC^[CT] HinfI 337 GAGTC G^A[ACGT]TC HphI 63 GGTGA GGTGA(8/7) Ksp632I 334 GAAGAG CTCTTC(1/4) MslI 259 CACGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 549 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 337 GAGTC GAGTC(4/5) PpuMI 119 AGGACCT [GA]G^G[AT]CC[CT] SacI 211 GAGCTC GAGCT^C SfaNI 29 GCATC GCATC(5/9) SmaI 276 CCCGGG CCC^GGG TaqI 340 TCGA T^CGA TthHB8I 340 TCGA T^CGA Van91I 549 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 276 CCCGGG C^CCGGG XmaI 276 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 370 GACGCC G[GA]^CG[CT]C 424 GGCGTC AluI 166 AGCT AG^CT 212 AGCT AspHI 211 GAGCTC G[AT]GC[AT]^C 469 GAGCAC AvaI 276 CCCGGG C^[CT]CG[GA]G 403 CCCGAG AvaII 120 GGACC G^G[AT]CC 503 GGACC BbsI 480 GAAGAC GAAGAC(2/6) 10 GTCTTC BpuAI 480 GAAGAC GAAGAC(2/6) 10 GTCTTC BsaHI 370 GACGCC G[GA]^CG[CT]C 424 GGCGTC BsiHKAI 211 GAGCTC G[AT]GC[AT]^C 469 GAGCAC BsoBI 276 CCCGGG C^[CT]CG[GA]G 403 CCCGAG BsrFI 22 GCCGGC [GA]^CCGG[CT] 356 GCCGGC BsrI 189 ACTGG ACTGG(1/-1) 549 CCAGT BstF5I 390 CATCC GGATG(2/0) 264 GGATG BstYI 78 GGATCC [GA]^GATC[CT] 432 GGATCT Cfr10I 22 GCCGGC [GA]^CCGG[CT] 356 GCCGGC DpnII 79 GATC ^GATC 433 GATC EaeI 24 CGGCCG [CT]^GGCC[GA] 302 CGGCCA FauI 19 CCCGC CCCGC(4/6) 82 CCCGC FokI 390 CATCC GGATG(9/13) 264 GGATG Hin1I 370 GACGCC G[GA]^CG[CT]C 424 GGCGTC MaeII 218 ACGT A^CGT 252 ACGT MboI 79 GATC ^GATC 433 GATC MflI 78 GGATCC [GA]^GATC[CT] 432 GGATCT MspA1I 69 CCGCTG C[AC]G^C[GT]G 160 CAGCGG NaeI 22 GCCGGC GCC^GGC 356 GCCGGC NdeII 79 GATC ^GATC 433 GATC NgoMIV 22 GCCGGC G^CCGGC 356 GCCGGC Sau3AI 79 GATC ^GATC 433 GATC XhoII 78 GGATCC [GA]^GATC[CT] 432 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AlwI ApaI BanII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TseI TspRI