################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AvaI AviII AvrII BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI FspI HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 998 GACGCC G[GA]^CG[CT]C AhdI 2534 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 2039 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2534 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2318 GTGCTC G[AT]GC[AT]^C AspI 2644 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1256 GGATCC G^GATCC BanI 1410 GGTGCC G^G[CT][GA]CC BclI 32 TGATCA T^GATCA BsaAI 2527 TACGTG [CT]AC^GT[GA] BsaBI 766 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 998 GACGCC G[GA]^CG[CT]C BsaI 2642 GAGACC GGTCTC(1/5) BsiHKAI 2318 GTGCTC G[AT]GC[AT]^C BsmI 1531 GCATTC GAATGC(1/-1) BspDI 110 ATCGAT AT^CGAT BspMI 1523 ACCTGC ACCTGC(4/8) BsrDI 2287 GCAATG GCAATG(2/0) BstYI 1256 GGATCC [GA]^GATC[CT] ClaI 110 ATCGAT AT^CGAT DraI 982 TTTAAA TTT^AAA Eam1105I 2534 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1785 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 347 ATGCAT ATGCA^T FauI 1860 CCCGC CCCGC(4/6) FbaI 32 TGATCA T^GATCA Hin1I 998 GACGCC G[GA]^CG[CT]C HindIII 211 AAGCTT A^AGCTT MamI 766 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 152 CAATTG C^AATTG MflI 1256 GGATCC [GA]^GATC[CT] MunI 152 CAATTG C^AATTG NdeI 1178 CATATG CA^TATG NsiI 347 ATGCAT ATGCA^T NspI 1053 GCATGT [GA]CATG^[CT] PflFI 2644 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2334 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1691 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SmlI 1116 CTCAAG C^T[CT][GA]AG SspI 1472 AATATT AAT^ATT Tth111I 2644 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1256 GGATCC [GA]^GATC[CT] XmnI 2039 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BbvCI 1769 CCTCAGC CCTCAGC(-5/-2) 2440 CCTCAGC BsgI 1763 CTGCAC GTGCAG(16/14) 2125 GTGCAG BstXI 1916 CCAAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2393 CCACAGCTGTGG CfoI 1782 GCGC GCG^C 2726 GCGC DraII 2259 GGGCCCT [GA]G^G[ACGT]CC[CT] 2334 AGGACCT DraIII 2492 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 2603 CACATCGTG DsaI 238 CCATGG C^C[GA][CT]GG 1221 CCATGG EcoO109I 2259 GGGCCCT [GA]G^G[ACGT]CC[CT] 2334 AGGACCT HaeII 1781 AGCGCC [GA]GCGC^[CT] 2725 GGCGCT HhaI 1782 GCGC GCG^C 2726 GCGC HinP1I 1782 GCGC G^CGC 2726 GCGC HphI 769 GGTGA GGTGA(8/7) 2566 TCACC NcoI 238 CCATGG C^CATGG 1221 CCATGG SapI 694 GAAGAGC GCTCTTC(1/4) 1063 GAAGAGC SbfI 1524 CCTGCAGG CCTGCA^GG 2134 CCTGCAGG Sse8387I 1524 CCTGCAGG CCTGCA^GG 2134 CCTGCAGG TaqI 111 TCGA T^CGA 163 TCGA TthHB8I 111 TCGA T^CGA 163 TCGA XcmI 1081 CCAGCCTTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1397 CCAGTAAGAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII EaeI EarI Eco81I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI