(Circular) (Six-base) MAPSORT of: pFN21AB0448.seq Check: 4855 from: 1 to: 7438 pFN21AB0448 7438 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5248 278 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4781 1937 Size: 2844 4594 Acc65I G'GTAC_C Cuts at: 4759 4759 Size: 7438 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 3100 3632 4436 4679 5501 6885 7153 Size: 653 815 532 804 243 822 1384 268 Cuts at: 7153 1632 Size: 1917 Fragments arranged by size: 1917 1384 822 815 804 653 532 268 243 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 6337 7314 1203 Size: 5134 977 1327 Fragments arranged by size: 5134 1327 977 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 4544 6167 4544 Size: 1623 5815 AleI CACnn'nnGTG Cuts at: 1166 4403 1166 Size: 3237 4201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4155 1184 Size: 2971 4467 AlwNI CAG_nnn'CTG Cuts at: 2803 3217 3596 4343 5181 6753 2803 Size: 414 379 747 838 1572 3488 Fragments arranged by size: 3488 1572 838 747 414 379 ApaI G_GGCC'C Cuts at: 4202 4268 4304 4202 Size: 66 36 7336 Fragments arranged by size: 7336 66 36 ApaLI G'TGCA_C Cuts at: 5495 6651 5495 Size: 1156 6282 ApoI r'AATT_y Cuts at: 1493 2180 2405 2750 2984 4348 4747 4973 5125 Size: 687 225 345 234 1364 399 226 152 Cuts at: 5125 7234 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1364 687 399 345 234 226 225 152 AseI AT'TA_AT Cuts at: 160 1032 5992 160 Size: 872 4960 1606 Fragments arranged by size: 4960 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 4763 1870 Size: 73 2820 4545 Fragments arranged by size: 4545 2820 73 BamHI G'GATC_C Cuts at: 3261 4768 5135 3261 Size: 1507 367 5564 Fragments arranged by size: 5564 1507 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3415 4759 6214 618 Size: 325 217 627 1628 1344 1455 1842 Fragments arranged by size: 1842 1628 1455 1344 627 325 217 BanII G_rGCy'C Cuts at: 1391 4202 4268 4304 4757 1391 Size: 2811 66 36 453 4072 Fragments arranged by size: 4072 2811 453 66 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2141 3381 3864 928 Size: 971 242 1240 483 4502 Fragments arranged by size: 4502 1240 971 483 242 BbvCI CC'TCA_GC Cuts at: 3775 4446 3775 Size: 671 6767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5629 5663 1903 Size: 34 3692 34 3678 Fragments arranged by size: 3692 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5330 6540 5330 Size: 1210 6228 BclI T'GATC_A Cuts at: 1548 2037 1548 Size: 489 6949 BfrBI ATG'CAT Cuts at: 2354 2354 Size: 7438 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6049 136 Size: 107 122 71 631 605 4377 1525 Fragments arranged by size: 4377 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7433 7433 Size: 7438 BlpI GC'TnA_GC Cuts at: 4855 4855 Size: 7438 Bme1580I G_kGCm'C Cuts at: 1790 3654 4202 4268 4304 5499 6655 1790 Size: 1864 548 66 36 1195 1156 2573 Fragments arranged by size: 2573 1864 1195 1156 548 66 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3497 5169 6127 Size: 416 53 83 399 24 2080 1672 958 Cuts at: 6127 442 Size: 1753 Fragments arranged by size: 2080 1753 1672 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3825 3825 Size: 7438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 2193 2603 2654 6098 1239 Size: 933 21 410 51 3444 2579 Fragments arranged by size: 3444 2579 933 410 51 21 Bpu10I CC'TnA_GC Cuts at: 3775 4446 3775 Size: 671 6767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3106 5563 6428 6726 3106 Size: 2457 865 298 3818 Fragments arranged by size: 3818 2457 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4641 6101 882 Size: 617 3142 1460 2219 Fragments arranged by size: 3142 2219 1460 617 BsaAI yAC'GTr Cuts at: 493 1204 4534 5229 493 Size: 711 3330 695 2702 Fragments arranged by size: 3330 2702 711 695 BsaBI GATnn'nnATC Cuts at: 2775 7432 2775 Size: 4657 2781 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3004 5245 5627 6262 Size: 53 83 186 936 1471 2241 382 635 Cuts at: 6262 275 Size: 1451 Fragments arranged by size: 2241 1471 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5871 6269 6543 6690 7101 5871 Size: 398 274 147 411 6208 Fragments arranged by size: 6208 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4638 4668 1178 Size: 30 165 30 3235 30 3948 Fragments arranged by size: 3948 3235 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2699 3812 3830 4720 1186 Size: 1513 1113 18 890 3904 Fragments arranged by size: 3904 1513 1113 890 18 BseYI C'CCAG_C Cuts at: 3745 4380 4721 6641 7137 3745 Size: 635 341 1920 496 4046 Fragments arranged by size: 4046 1920 635 496 341 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3753 4151 1163 Size: 573 2017 398 4450 Fragments arranged by size: 4450 2017 573 398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4888 5649 6677 1540 Size: 275 186 2887 761 1028 2301 Fragments arranged by size: 2887 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4327 5499 5584 6655 4327 Size: 1172 85 1071 5110 Fragments arranged by size: 5110 1172 1071 85 BsmI GAATG_Cn' Cuts at: 3536 4949 5042 3536 Size: 1413 93 5932 Fragments arranged by size: 5932 1413 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2548 2908 4610 2548 Size: 360 1702 5376 Fragments arranged by size: 5376 1702 360 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3654 4202 4268 4304 4327 4757 5499 Size: 399 1864 548 66 36 23 430 742 Cuts at: 5499 5584 6655 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1864 1071 742 548 430 399 85 66 36 23 BspEI T'CCGG_A Cuts at: 7101 7101 Size: 7438 BspHI T'CATG_A Cuts at: 5327 7057 5327 Size: 1730 5708 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3537 4793 844 Size: 2693 1256 3489 Fragments arranged by size: 3489 2693 1256 BsrBI CCG'CTC Cuts at: 1394 1823 5325 1394 Size: 429 3502 3507 Fragments arranged by size: 3507 3502 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4299 5933 6107 65 Size: 1183 3051 1634 174 1396 Fragments arranged by size: 3051 1634 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6082 6269 1700 Size: 110 4272 187 2869 Fragments arranged by size: 4272 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7438 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7438 BssSI C'ACGA_G Cuts at: 5498 6510 5498 Size: 1012 6426 BstAPI GCAn_nnn'nTGC Cuts at: 794 2647 3596 3626 794 Size: 1853 949 30 4606 Fragments arranged by size: 4606 1853 949 30 BstBI TT'CG_AA Cuts at: 6242 6242 Size: 7438 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7438 BstXI CCAn_nnnn'nTGG Cuts at: 3928 4405 3928 Size: 477 6961 BstYI r'GATC_y Cuts at: 1972 3261 4768 5135 5520 5537 6989 7075 7087 Size: 1289 1507 367 385 17 1452 86 12 Cuts at: 7087 7433 1972 Size: 346 1977 Fragments arranged by size: 1977 1507 1452 1289 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2660 3810 4207 2660 Size: 1150 397 5891 Fragments arranged by size: 5891 1150 397 BtgI C'CryG_G Cuts at: 513 1065 2004 2243 3226 513 Size: 552 939 239 983 4725 Fragments arranged by size: 4725 983 939 552 239 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6699 Fragments arranged by size: 6699 683 56 BtsI GCAGTG_nn' Cuts at: 4954 5748 5768 4954 Size: 794 20 6624 Fragments arranged by size: 6624 794 20 ClaI AT'CG_AT Cuts at: 1554 2116 1554 Size: 562 6876 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3892 3927 529 Size: 35 3328 35 4040 Fragments arranged by size: 4040 3328 35 35 DraI TTT'AAA Cuts at: 2989 4742 2989 Size: 1753 5685 DraIII CAC_nnn'GTG Cuts at: 4502 4613 4502 Size: 111 7327 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6445 809 Size: 507 5129 1802 Fragments arranged by size: 5129 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3187 3631 3973 Size: 54 1425 50 275 18 1357 444 342 Cuts at: 3973 4885 5774 7403 8 Size: 912 889 1629 43 Fragments arranged by size: 1629 1425 1357 912 889 444 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2694 2836 3063 3987 4655 4660 5368 1353 Size: 1341 142 227 924 668 5 708 3423 Fragments arranged by size: 3423 1341 924 708 668 227 142 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6025 6399 6545 387 Size: 1064 138 4436 374 146 1280 Fragments arranged by size: 4436 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2193 2285 2603 2654 3100 3632 Size: 393 540 21 92 318 51 446 532 Cuts at: 3632 4436 4679 5501 6098 6885 7153 1239 Size: 804 243 822 597 787 268 1524 Fragments arranged by size: 1524 822 804 787 597 540 532 446 393 318 268 243 92 51 21 EcoNI CCTnn'n_nnAGG Cuts at: 3794 3794 Size: 7438 EcoO109I rG'GnC_Cy Cuts at: 4265 4340 4265 Size: 75 7363 EcoRI G'AATT_C Cuts at: 4747 5125 4747 Size: 378 7060 FspI TGC'GCA Cuts at: 5944 5944 Size: 7438 HaeII r_GCGC'y Cuts at: 1678 3790 4734 6585 1678 Size: 2112 944 1851 2531 Fragments arranged by size: 2531 2112 1851 944 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2813 2845 2908 2940 3104 Size: 32 135 32 1241 32 63 32 164 Cuts at: 3104 3136 3719 3749 3751 3781 3899 3931 4530 Size: 32 583 30 2 30 118 32 599 Cuts at: 4530 4562 4638 4670 4701 4733 6079 6111 6153 Size: 32 76 32 31 32 1346 32 42 Cuts at: 6153 6185 1373 Size: 32 2626 Fragments arranged by size: 2626 1346 1241 599 583 164 135 118 76 63 42 32 32 32 32 32 32 32 32 32 32 32 31 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 2224 2496 3469 4782 5028 669 Size: 988 281 286 272 973 1313 246 3079 Fragments arranged by size: 3079 1313 988 973 286 281 272 246 HindIII A'AGCT_T Cuts at: 748 2216 748 Size: 1468 5970 HpaI GTT'AAC Cuts at: 5028 5028 Size: 7438 KpnI G_GTAC'C Cuts at: 4763 4763 Size: 7438 MfeI C'AATT_G Cuts at: 2157 5037 2157 Size: 2880 4558 MluI A'CGCG_T Cuts at: 7314 7314 Size: 7438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2353 2743 4534 4624 6552 6736 1305 Size: 1048 390 1791 90 1928 184 2007 Fragments arranged by size: 2007 1928 1791 1048 390 184 90 MscI TGG'CCA Cuts at: 10 64 1489 1832 3189 3633 3975 10 Size: 54 1425 343 1357 444 342 3473 Fragments arranged by size: 3473 1425 1357 444 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 3965 3969 4403 5396 5755 5914 518 Size: 648 2799 4 434 993 359 159 2042 Fragments arranged by size: 2799 2042 993 648 434 359 159 4 MspA1I CmG'CkG Cuts at: 1606 3353 3868 4216 4403 4725 4854 5531 6679 Size: 1747 515 348 187 322 129 677 1148 Cuts at: 6679 6924 1606 Size: 245 2120 Fragments arranged by size: 2120 1747 1148 677 515 348 322 245 187 129 NcoI C'CATG_G Cuts at: 513 1065 2004 2243 3226 513 Size: 552 939 239 983 4725 Fragments arranged by size: 4725 983 939 552 239 NdeI CA'TA_TG Cuts at: 387 3184 387 Size: 2797 4641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NsiI A_TGCA'T Cuts at: 2356 2356 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 3062 4796 6341 1132 Size: 97 1833 1734 1545 2229 Fragments arranged by size: 2229 1833 1734 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7438 PciI A'CATG_T Cuts at: 6337 6337 Size: 7438 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7438 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5507 5539 7020 7052 5507 Size: 32 1481 32 5893 Fragments arranged by size: 5893 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4340 4340 Size: 7438 PshAI GACnn'nnGTC Cuts at: 1613 3700 1613 Size: 2087 5351 PsiI TTA'TAA Cuts at: 2748 5008 2748 Size: 2260 5178 PspOMI G'GGCC_C Cuts at: 4198 4264 4300 4198 Size: 66 36 7336 Fragments arranged by size: 7336 66 36 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2784 2816 2784 Size: 32 7406 PstI C_TGCA'G Cuts at: 830 3534 3592 3690 3991 4144 4441 4790 830 Size: 2704 58 98 301 153 297 349 3478 Fragments arranged by size: 3478 2704 349 301 297 153 98 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 PvuII CAG'CTG Cuts at: 3353 4216 4403 3353 Size: 863 187 6388 Fragments arranged by size: 6388 863 187 SalI G'TCGA_C Cuts at: 1936 4780 1936 Size: 2844 4594 SapI GCTCTTCn'nnn_ Cuts at: 2694 3063 2694 Size: 369 7069 SbfI CC_TGCA'GG Cuts at: 3534 4144 4790 3534 Size: 610 646 6182 Fragments arranged by size: 6182 646 610 ScaI AGT'ACT Cuts at: 5686 7398 5686 Size: 1712 5726 SfcI C'TryA_G Cuts at: 826 1045 2369 3358 3530 3588 3686 3987 4140 Size: 219 1324 989 172 58 98 301 153 Cuts at: 4140 4437 4786 5921 6602 6793 826 Size: 297 349 1135 681 191 1471 Fragments arranged by size: 1471 1324 1135 989 681 349 301 297 219 191 172 153 98 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 4765 1872 Size: 2893 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3121 5542 6443 6705 820 Size: 197 926 1178 2421 901 262 1553 Fragments arranged by size: 2421 1553 1178 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4796 1132 Size: 3664 3774 SspI AAT'ATT Cuts at: 5 52 3479 5362 5 Size: 47 3427 1883 2081 Fragments arranged by size: 3427 2081 1883 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7438 StyI C'CwwG_G Cuts at: 513 1065 2004 2243 3226 4366 4642 4877 513 Size: 552 939 239 983 1140 276 235 3074 Fragments arranged by size: 3074 1140 983 939 552 276 239 235 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2266 3516 5465 5482 5635 5820 6265 Size: 1255 686 1250 1949 17 153 185 445 Cuts at: 6265 325 Size: 1498 Fragments arranged by size: 1949 1498 1255 1250 686 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4162 5684 7396 Size: 274 80 33 51 1443 2185 1522 1712 Cuts at: 7396 96 Size: 138 Fragments arranged by size: 2185 1712 1522 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7398 7430 7398 Size: 32 7406 Tth111I GACn'n_nGTC Cuts at: 1316 4652 1316 Size: 3336 4102 XbaI T'CTAG_A Cuts at: 4774 4774 Size: 7438 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3093 3409 3093 Size: 316 7122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7438 XmaI C'CCGG_G Cuts at: 1870 4763 1870 Size: 2893 4545 XmnI GAAnn'nnTTC Cuts at: 4048 5567 4048 Size: 1519 5919 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5246 276 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI