(Linear) (Six-base) MAPSORT of: KIBB0448.seq Check: 502 from: 1 to: 2733 KIBB0448.orf With 182 enzymes: SgfI * July 3, 2012 16:01 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 1095 1627 2431 2674 2733 Size: 280 815 532 804 243 59 Fragments arranged by size: 815 804 532 280 243 59 AhdI GACnn_n'nnGTC Cuts at: 0 2539 2733 Size: 2539 194 AleI CACnn'nnGTG Cuts at: 0 2398 2733 Size: 2398 335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2150 2733 Size: 2150 583 AlwNI CAG_nnn'CTG Cuts at: 0 798 1212 1591 2338 2733 Size: 798 414 379 747 395 Fragments arranged by size: 798 747 414 395 379 ApaI G_GGCC'C Cuts at: 0 2197 2263 2299 2733 Size: 2197 66 36 434 Fragments arranged by size: 2197 434 66 36 ApoI r'AATT_y Cuts at: 0 175 400 745 979 2343 2733 Size: 175 225 345 234 1364 390 Fragments arranged by size: 1364 390 345 234 225 175 BamHI G'GATC_C Cuts at: 0 1256 2733 Size: 1256 1477 BanI G'GyrC_C Cuts at: 0 1410 2733 Size: 1410 1323 BanII G_rGCy'C Cuts at: 0 2197 2263 2299 2733 Size: 2197 66 36 434 Fragments arranged by size: 2197 434 66 36 BbsI GAAGACnn'nnnn_ Cuts at: 0 136 1376 1859 2733 Size: 136 1240 483 874 Fragments arranged by size: 1240 874 483 136 BbvCI CC'TCA_GC Cuts at: 0 1770 2441 2733 Size: 1770 671 292 Fragments arranged by size: 1770 671 292 BclI T'GATC_A Cuts at: 0 32 2733 Size: 32 2701 BfrBI ATG'CAT Cuts at: 0 349 2733 Size: 349 2384 Bme1580I G_kGCm'C Cuts at: 0 1649 2197 2263 2299 2733 Size: 1649 548 66 36 434 Fragments arranged by size: 1649 548 434 66 36 BmrI ACTGGGnnnn_n' Cuts at: 0 1492 2733 Size: 1492 1241 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1820 2733 Size: 1820 913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 167 188 598 649 2733 Size: 167 21 410 51 2084 Fragments arranged by size: 2084 410 167 51 21 Bpu10I CC'TnA_GC Cuts at: 0 1770 2441 2733 Size: 1770 671 292 Fragments arranged by size: 1770 671 292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1101 2733 Size: 1101 1632 BsaI GGTCTCn'nnnn_ Cuts at: 0 2636 2733 Size: 2636 97 BsaAI yAC'GTr Cuts at: 0 2529 2733 Size: 2529 204 BsaBI GATnn'nnATC Cuts at: 0 770 2733 Size: 770 1963 BsaHI Gr'CG_yC Cuts at: 0 999 2733 Size: 999 1734 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2633 2663 2733 Size: 2633 30 70 Fragments arranged by size: 2633 70 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 694 1807 1825 2715 2733 Size: 694 1113 18 890 18 Fragments arranged by size: 1113 890 694 18 18 BseYI C'CCAG_C Cuts at: 0 1740 2375 2716 2733 Size: 1740 635 341 17 Fragments arranged by size: 1740 635 341 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1748 2146 2733 Size: 1748 398 587 Fragments arranged by size: 1748 587 398 BsiHKAI G_wGCw'C Cuts at: 0 2322 2733 Size: 2322 411 BsmI GAATG_Cn' Cuts at: 0 1531 2733 Size: 1531 1202 BsmBI CGTCTCn'nnnn_ Cuts at: 0 543 903 2605 2733 Size: 543 360 1702 128 Fragments arranged by size: 1702 543 360 128 Bsp1286I G_dGCh'C Cuts at: 0 1649 2197 2263 2299 2322 2733 Size: 1649 548 66 36 23 411 Fragments arranged by size: 1649 548 411 66 36 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1532 2733 Size: 1532 1201 BsrDI GCAATG_nn' Cuts at: 0 2294 2733 Size: 2294 439 BstAPI GCAn_nnn'nTGC Cuts at: 0 642 1591 1621 2733 Size: 642 949 30 1112 Fragments arranged by size: 1112 949 642 30 BstXI CCAn_nnnn'nTGG Cuts at: 0 1923 2400 2733 Size: 1923 477 333 Fragments arranged by size: 1923 477 333 BstYI r'GATC_y Cuts at: 0 1256 2733 Size: 1256 1477 Bsu36I CC'TnA_GG Cuts at: 0 655 1805 2202 2733 Size: 655 1150 397 531 Fragments arranged by size: 1150 655 531 397 BtgI C'CryG_G Cuts at: 0 238 1221 2733 Size: 238 983 1512 Fragments arranged by size: 1512 983 238 ClaI AT'CG_AT Cuts at: 0 111 2733 Size: 111 2622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1887 1922 2733 Size: 1887 35 811 Fragments arranged by size: 1887 811 35 DraI TTT'AAA Cuts at: 0 984 2733 Size: 984 1749 DraIII CAC_nnn'GTG Cuts at: 0 2497 2608 2733 Size: 2497 111 125 Fragments arranged by size: 2497 125 111 EaeI y'GGCC_r Cuts at: 0 1182 1626 1968 2733 Size: 1182 444 342 765 Fragments arranged by size: 1182 765 444 342 EarI CTCTTCn'nnn_ Cuts at: 0 689 831 1058 1982 2650 2655 2733 Size: 689 142 227 924 668 5 78 Fragments arranged by size: 924 689 668 227 142 78 5 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 167 188 280 598 649 1095 1627 2431 Size: 167 21 92 318 51 446 532 804 Cuts at: 2431 2674 2733 Size: 243 59 Fragments arranged by size: 804 532 446 318 243 167 92 59 51 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 1789 2733 Size: 1789 944 EcoO109I rG'GnC_Cy Cuts at: 0 2260 2335 2733 Size: 2260 75 398 Fragments arranged by size: 2260 398 75 HaeII r_GCGC'y Cuts at: 0 1785 2729 2733 Size: 1785 944 4 Fragments arranged by size: 1785 944 4 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 808 840 903 935 1099 1131 1714 1744 Size: 808 32 63 32 164 32 583 30 Cuts at: 1744 1746 1776 1894 1926 2525 2557 2633 2665 Size: 2 30 118 32 599 32 76 32 Cuts at: 2665 2696 2728 2733 Size: 31 32 5 Fragments arranged by size: 808 599 583 164 118 76 63 32 32 32 32 32 32 32 31 30 30 5 2 HincII GTy'rAC Cuts at: 0 219 491 1464 2733 Size: 219 272 973 1269 Fragments arranged by size: 1269 973 272 219 HindIII A'AGCT_T Cuts at: 0 211 2733 Size: 211 2522 MfeI C'AATT_G Cuts at: 0 152 2733 Size: 152 2581 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 348 738 2529 2619 2733 Size: 348 390 1791 90 114 Fragments arranged by size: 1791 390 348 114 90 MscI TGG'CCA Cuts at: 0 1184 1628 1970 2733 Size: 1184 444 342 763 Fragments arranged by size: 1184 763 444 342 MslI CAynn'nnrTG Cuts at: 0 1960 1964 2398 2733 Size: 1960 4 434 335 Fragments arranged by size: 1960 434 335 4 MspA1I CmG'CkG Cuts at: 0 1348 1863 2211 2398 2720 2733 Size: 1348 515 348 187 322 13 Fragments arranged by size: 1348 515 348 322 187 13 NcoI C'CATG_G Cuts at: 0 238 1221 2733 Size: 238 983 1512 Fragments arranged by size: 1512 983 238 NdeI CA'TA_TG Cuts at: 0 1179 2733 Size: 1179 1554 NsiI A_TGCA'T Cuts at: 0 351 2733 Size: 351 2382 NspI r_CATG'y Cuts at: 0 1057 2733 Size: 1057 1676 PpuMI rG'GwC_Cy Cuts at: 0 2335 2733 Size: 2335 398 PshAI GACnn'nnGTC Cuts at: 0 1695 2733 Size: 1695 1038 PsiI TTA'TAA Cuts at: 0 743 2733 Size: 743 1990 PspOMI G'GGCC_C Cuts at: 0 2193 2259 2295 2733 Size: 2193 66 36 438 Fragments arranged by size: 2193 438 66 36 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 779 811 2733 Size: 779 32 1922 Fragments arranged by size: 1922 779 32 PstI C_TGCA'G Cuts at: 0 1529 1587 1685 1986 2139 2436 2733 Size: 1529 58 98 301 153 297 297 Fragments arranged by size: 1529 301 297 297 153 98 58 PvuII CAG'CTG Cuts at: 0 1348 2211 2398 2733 Size: 1348 863 187 335 Fragments arranged by size: 1348 863 335 187 SapI GCTCTTCn'nnn_ Cuts at: 0 689 1058 2733 Size: 689 369 1675 Fragments arranged by size: 1675 689 369 SbfI CC_TGCA'GG Cuts at: 0 1529 2139 2733 Size: 1529 610 594 Fragments arranged by size: 1529 610 594 SfcI C'TryA_G Cuts at: 0 364 1353 1525 1583 1681 1982 2135 2432 Size: 364 989 172 58 98 301 153 297 Cuts at: 2432 2733 Size: 301 Fragments arranged by size: 989 364 301 301 297 172 153 98 58 SmlI C'TyrA_G Cuts at: 0 1116 2733 Size: 1116 1617 SspI AAT'ATT Cuts at: 0 1474 2733 Size: 1474 1259 StyI C'CwwG_G Cuts at: 0 238 1221 2361 2637 2733 Size: 238 983 1140 276 96 Fragments arranged by size: 1140 983 276 238 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 261 1511 2733 Size: 261 1250 1222 Fragments arranged by size: 1250 1222 261 TatI w'GTAC_w Cuts at: 0 2157 2733 Size: 2157 576 Tth111I GACn'n_nGTC Cuts at: 0 2647 2733 Size: 2647 86 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1088 1404 2733 Size: 1088 316 1329 Fragments arranged by size: 1329 1088 316 XmnI GAAnn'nnTTC Cuts at: 0 2043 2733 Size: 2043 690 Enzymes that do cut: AcuI AhdI AleI AlfI AlwNI ApaI ApoI BamHI BanI BanII BbsI BbvCI BclI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI Bsu36I BtgI ClaI CspCI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SapI SbfI SfcI SmlI SspI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BglI BglII BlpI BmgBI BmtI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PspXI PvuI RsrII SacI SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI XbaI XhoI XmaI ZraI