################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AclI AcsI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BclI BfaI BfrI BlnI BsaAI BsaBI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BsrI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI MaeI MaeII MaeIII MamI MfeI MseI MunI NarI NcoI NdeI NheI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 566 GGTACC G^GTACC AccIII 730 TCCGGA T^CCGGA AcyI 600 GACGCC G[GA]^CG[CT]C AflIII 1076 ACGCGT A^C[GA][CT]GT AhdI 825 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 787 GGGCCC GGGCC^C Asp700I 756 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 566 GGTACC G^GTACC AspEI 825 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1092 GAGCTC G[AT]GC[AT]^C AvaII 863 GGACC G^G[AT]CC BalI 896 TGGCCA TGG^CCA BciVI 117 GTATCC GTATCC(6/5) BglII 740 AGATCT A^GATCT BsaHI 600 GACGCC G[GA]^CG[CT]C BsaI 717 GAGACC GGTCTC(1/5) BseAI 730 TCCGGA T^CCGGA BsiHKAI 1092 GAGCTC G[AT]GC[AT]^C BsmFI 644 GGGAC GGGAC(10/14) BsmI 40 GCATTC GAATGC(1/-1) BspEI 730 TCCGGA T^CCGGA BsrDI 16 GCAATG GCAATG(2/0) BssSI 714 CACGAG CACGAG(-5/-1) BstBI 763 TTCGAA TT^CGAA Eam1105I 825 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 667 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 298 GATATC GAT^ATC Hin1I 600 GACGCC G[GA]^CG[CT]C KpnI 566 GGTACC GGTAC^C MluI 1076 ACGCGT A^CGCGT MluNI 896 TGGCCA TGG^CCA MroI 730 TCCGGA T^CCGGA MscI 896 TGGCCA TGG^CCA MslI 318 CATGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NotI 433 GCGGCCGC GC^GGCCGC NspV 763 TTCGAA TT^CGAA PflMI 605 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1021 CAGCTG CAG^CTG SacI 1092 GAGCTC GAGCT^C SfiI 327 GGCCTCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 763 TTCGAA TT^CGAA SmaI 517 CCCGGG CCC^GGG SmlI 937 CTCAAG C^T[CT][GA]AG TaqI 764 TCGA T^CGA TthHB8I 764 TCGA T^CGA Van91I 605 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 517 CCCGGG C^CCGGG XmaI 517 CCCGGG C^CCGGG XmnI 756 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BamHI 676 GGATCC G^GATCC 990 GGATCC BbsI 93 GAAGAC GAAGAC(2/6) 756 GAAGAC BlpI 48 GCTCAGC GC^T[ACGT]AGC 583 GCTGAGC BpmI 639 CTGGAG CTGGAG(16/14) 751 CTGGAG Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC 583 GCTGAGC BpuAI 93 GAAGAC GAAGAC(2/6) 756 GAAGAC BsaWI 361 TCCGGT [AT]^CCGG[AT] 730 TCCGGA BsgI 73 CTGCAC GTGCAG(16/14) 1072 CTGCAC BsmAI 717 GAGAC GTCTC(1/5) 925 GAGAC CelII 48 GCTCAGC GC^T[ACGT]AGC 583 GCTGAGC DraII 786 GGGGCCC [GA]G^G[ACGT]CC[CT] 943 GGGGCCT EcoO109I 786 GGGGCCC [GA]G^G[ACGT]CC[CT] 943 GGGGCCT HaeII 538 AGCGCC [GA]GCGC^[CT] 932 AGCGCC HinfI 831 GAGTC G^A[ACGT]TC 919 GACTC NaeI 133 GCCGGC GCC^GGC 308 GCCGGC NgoMIV 133 GCCGGC G^CCGGC 308 GCCGGC NlaIII 81 CATG CATG^ 318 CATG PleI 919 GACTC GAGTC(4/5) 831 GAGTC SfaNI 624 GATGC GCATC(5/9) 290 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI BanI BanII BbvI BcnI BglI BmyI BsaJI BseRI BsiEI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EarI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI Ksp632I KspI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV RsaI SacII Sau3AI Sau96I ScrFI TseI XhoII