(Circular) (Six-base) MAPSORT of: pFN21AB7933.seq Check: 2683 from: 1 to: 5806 pFN21AB7933 5806 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3149 1937 Size: 1212 4594 Acc65I G'GTAC_C Cuts at: 2571 3127 2571 Size: 556 5250 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2518 3869 5253 5521 1632 Size: 608 278 1351 1384 268 1917 Fragments arranged by size: 1917 1384 1351 608 278 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 3081 4705 5682 1203 Size: 1878 1624 977 1327 Fragments arranged by size: 1878 1624 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 2835 4535 2835 Size: 1700 4106 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5806 AlwNI CAG_nnn'CTG Cuts at: 3549 5121 3549 Size: 1572 4234 ApaI G_GGCC'C Cuts at: 2796 2796 Size: 5806 ApaLI G'TGCA_C Cuts at: 3863 5019 3863 Size: 1156 4650 ApoI r'AATT_y Cuts at: 1493 3115 3341 3493 5602 1493 Size: 1622 226 152 2109 1697 Fragments arranged by size: 2109 1697 1622 226 152 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 2335 2522 2795 3131 1870 Size: 73 392 187 273 336 4545 Fragments arranged by size: 4545 392 336 273 187 73 BamHI G'GATC_C Cuts at: 2681 2995 3136 3503 2681 Size: 314 141 367 4984 Fragments arranged by size: 4984 367 314 141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2198 2571 3127 4582 Size: 325 217 627 243 168 373 556 1455 Cuts at: 4582 618 Size: 1842 Fragments arranged by size: 1842 1455 627 556 373 325 243 217 168 BanII G_rGCy'C Cuts at: 1391 2244 2507 2529 2639 2796 2806 3101 3125 Size: 853 263 22 110 157 10 295 24 Cuts at: 3125 1391 Size: 4072 Fragments arranged by size: 4072 853 295 263 157 110 24 22 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2105 2768 928 Size: 971 206 663 3966 Fragments arranged by size: 3966 971 663 206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2133 3698 4908 2133 Size: 1565 1210 3031 Fragments arranged by size: 3031 1565 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2339 2426 2524 Size: 107 122 71 631 605 667 87 98 Cuts at: 2524 2900 4417 136 Size: 376 1517 1525 Fragments arranged by size: 1525 1517 667 631 605 376 122 107 98 87 71 BglII A'GATC_T Cuts at: 2745 5801 2745 Size: 3056 2750 BlpI GC'TnA_GC Cuts at: 2054 2589 3223 2054 Size: 535 634 4637 Fragments arranged by size: 4637 634 535 Bme1580I G_kGCm'C Cuts at: 1790 2033 2201 2796 3867 5023 1790 Size: 243 168 595 1071 1156 2573 Fragments arranged by size: 2573 1156 1071 595 243 168 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5806 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2665 2777 4466 1239 Size: 1426 112 1689 2579 Fragments arranged by size: 2579 1689 1426 112 Bpu10I CC'TnA_GC Cuts at: 2309 2501 2309 Size: 192 5614 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2927 3931 4796 5094 2927 Size: 1004 865 298 3639 Fragments arranged by size: 3639 1004 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2716 4469 882 Size: 617 1217 1753 2219 Fragments arranged by size: 2219 1753 1217 617 BsaAI yAC'GTr Cuts at: 493 1204 3597 493 Size: 711 2393 2702 Fragments arranged by size: 2702 2393 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2606 3613 3995 4630 Size: 53 83 186 936 1073 1007 382 635 Cuts at: 4630 275 Size: 1451 Fragments arranged by size: 1451 1073 1007 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2366 2735 4239 4637 4911 5058 5469 2366 Size: 369 1504 398 274 147 411 2703 Fragments arranged by size: 2703 1504 411 398 369 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5581 Fragments arranged by size: 5581 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2140 2143 2164 2365 2368 2371 2395 1186 Size: 954 3 21 201 3 3 24 4597 Fragments arranged by size: 4597 954 201 24 21 3 3 3 BseYI C'CCAG_C Cuts at: 2540 5009 5505 2540 Size: 2469 496 2841 Fragments arranged by size: 2841 2469 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 3062 1163 Size: 573 327 999 3907 Fragments arranged by size: 3907 999 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2280 2442 2553 3256 4017 5045 Size: 275 186 279 162 111 703 761 1028 Cuts at: 5045 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 703 279 275 186 162 111 BsiHKAI G_wGCw'C Cuts at: 3101 3867 3952 5023 3101 Size: 766 85 1071 3884 Fragments arranged by size: 3884 1071 766 85 BsmI GAATG_Cn' Cuts at: 2045 3317 3410 2045 Size: 1272 93 4441 Fragments arranged by size: 4441 1272 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2201 2244 2507 2529 2639 2796 Size: 399 243 168 43 263 22 110 157 Cuts at: 2796 2806 3101 3125 3867 3952 5023 1391 Size: 10 295 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 295 263 243 168 157 110 85 43 24 22 10 BspEI T'CCGG_A Cuts at: 2735 5469 2735 Size: 2734 3072 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3161 844 Size: 2317 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3693 1394 Size: 429 1870 3507 Fragments arranged by size: 3507 1870 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2028 4301 4475 65 Size: 1183 780 2273 174 1396 Fragments arranged by size: 2273 1396 1183 780 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 2313 2568 4450 4637 1700 Size: 110 328 175 255 1882 187 2869 Fragments arranged by size: 2869 1882 328 255 187 175 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5806 BssSI C'ACGA_G Cuts at: 2719 3866 4878 2719 Size: 1147 1012 3647 Fragments arranged by size: 3647 1147 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5806 BstBI TT'CG_AA Cuts at: 2769 4610 2769 Size: 1841 3965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstXI CCAn_nnnn'nTGG Cuts at: 2329 2584 2692 2329 Size: 255 108 5443 Fragments arranged by size: 5443 255 108 BstYI r'GATC_y Cuts at: 1972 2681 2745 2995 3136 3503 3888 3905 5357 Size: 709 64 250 141 367 385 17 1452 Cuts at: 5357 5443 5455 5801 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 709 385 367 346 250 141 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2232 2328 2424 2436 2547 2553 2562 Size: 552 1167 96 96 12 111 6 9 Cuts at: 2562 2904 513 Size: 342 3415 Fragments arranged by size: 3415 1167 552 342 111 96 96 12 9 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5067 Fragments arranged by size: 5067 683 56 BtsI GCAGTG_nn' Cuts at: 3322 4116 4136 3322 Size: 794 20 4992 Fragments arranged by size: 4992 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2088 2229 Size: 54 1425 50 275 18 240 18 141 Cuts at: 2229 2277 2439 2475 2550 2556 2599 2901 2907 Size: 48 162 36 75 6 43 302 6 Cuts at: 2907 3253 4142 5771 8 Size: 346 889 1629 43 Fragments arranged by size: 1629 1425 889 346 302 275 240 162 141 75 54 50 48 43 43 36 18 18 6 6 EagI C'GGCC_G Cuts at: 1537 1812 2277 2439 2550 3253 1537 Size: 275 465 162 111 703 4090 Fragments arranged by size: 4090 703 465 275 162 111 EarI CTCTTCn'nnn_ Cuts at: 1353 2389 2702 2961 3736 1353 Size: 1036 313 259 775 3423 Fragments arranged by size: 3423 1036 775 313 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2344 2383 2398 4393 4767 4913 Size: 1064 138 755 39 15 1995 374 146 Cuts at: 4913 387 Size: 1280 Fragments arranged by size: 1995 1280 1064 755 374 146 138 39 15 EcoICRI GAG'CTC Cuts at: 3099 3099 Size: 5806 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2518 2665 2777 3869 4466 5253 Size: 393 608 278 147 112 1092 597 787 Cuts at: 5253 5521 1239 Size: 268 1524 Fragments arranged by size: 1524 1092 787 608 597 393 278 268 147 112 EcoNI CCTnn'n_nnAGG Cuts at: 2676 2676 Size: 5806 EcoO109I rG'GnC_Cy Cuts at: 2792 2949 2792 Size: 157 5649 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 EcoRV GAT'ATC Cuts at: 2305 2305 Size: 5806 FspI TGC'GCA Cuts at: 4312 4312 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 2547 2941 4953 1678 Size: 869 394 2012 2531 Fragments arranged by size: 2531 2012 869 394 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2730 2762 2821 2853 2987 Size: 32 135 32 1158 32 59 32 134 Cuts at: 2987 3019 3086 3118 4447 4479 4521 4553 1373 Size: 32 67 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1329 1158 135 134 67 59 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 3396 669 Size: 988 281 1212 246 3079 Fragments arranged by size: 3079 1212 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KpnI G_GTAC'C Cuts at: 2575 3131 2575 Size: 556 5250 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 3081 5682 3081 Size: 2601 3205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2673 4920 5104 1305 Size: 1368 2247 184 2007 Fragments arranged by size: 2247 2007 1368 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2903 10 Size: 54 1425 343 1071 2913 Fragments arranged by size: 2913 1425 1071 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 3764 4123 4282 518 Size: 648 1161 1437 359 159 2042 Fragments arranged by size: 2042 1437 1161 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2131 2426 2438 2549 2584 3028 3054 Size: 501 24 295 12 111 35 444 26 Cuts at: 3054 3222 3899 5047 5292 1606 Size: 168 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 501 444 295 245 168 111 35 26 24 12 NaeI GCC'GGC Cuts at: 2140 2315 2140 Size: 175 5631 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5254 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NgoMIV G'CCGG_C Cuts at: 2138 2313 2138 Size: 175 5631 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 2439 3253 2439 Size: 814 4992 NspI r_CATG'y Cuts at: 1132 1229 3164 4709 1132 Size: 97 1935 1545 2229 Fragments arranged by size: 2229 1935 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 2616 1108 Size: 1508 4298 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PspOMI G'GGCC_C Cuts at: 2792 2792 Size: 5806 PstI C_TGCA'G Cuts at: 830 3158 830 Size: 2328 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 PvuII CAG'CTG Cuts at: 3028 3028 Size: 5806 SacI G_AGCT'C Cuts at: 3101 3101 Size: 5806 SacII CC_GC'GG Cuts at: 2427 2439 2550 2427 Size: 12 111 5683 Fragments arranged by size: 5683 111 12 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 3154 4289 4970 5161 826 Size: 219 2109 1135 681 191 1471 Fragments arranged by size: 2109 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2339 2339 Size: 5806 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 2524 3133 1872 Size: 652 609 4545 Fragments arranged by size: 4545 652 609 SmlI C'TyrA_G Cuts at: 820 1017 1943 2942 3910 4811 5073 820 Size: 197 926 999 968 901 262 1553 Fragments arranged by size: 1553 999 968 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 3164 1132 Size: 2032 3774 SrfI GCCC'GGGC Cuts at: 2524 2524 Size: 5806 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5806 StyI C'CwwG_G Cuts at: 513 1065 3245 513 Size: 552 2180 3074 Fragments arranged by size: 3074 2180 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2216 3833 3850 4003 4188 4633 325 Size: 1255 636 1617 17 153 185 445 1498 Fragments arranged by size: 1617 1498 1255 636 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5766 5798 5766 Size: 32 5774 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 2522 3131 1870 Size: 652 609 4545 Fragments arranged by size: 4545 652 609 XmnI GAAnn'nnTTC Cuts at: 2765 3935 2765 Size: 1170 4636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfoI SwaI