(Linear) (Six-base) MAPSORT of: KIBB7933.seq Check: 268 from: 1 to: 1101 KIBB7933.orf With 182 enzymes: SgfI * July 3, 2012 16:32 .. Acc65I G'GTAC_C Cuts at: 0 566 1101 Size: 566 535 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 513 1101 Size: 235 278 588 Fragments arranged by size: 588 278 235 AflIII A'CryG_T Cuts at: 0 1076 1101 Size: 1076 25 AhdI GACnn_n'nnGTC Cuts at: 0 830 1101 Size: 830 271 ApaI G_GGCC'C Cuts at: 0 791 1101 Size: 791 310 AvaI C'yCGr_G Cuts at: 0 330 517 790 1101 Size: 330 187 273 311 Fragments arranged by size: 330 311 273 187 BamHI G'GATC_C Cuts at: 0 676 990 1101 Size: 676 314 111 Fragments arranged by size: 676 314 111 BanI G'GyrC_C Cuts at: 0 25 193 566 1101 Size: 25 168 373 535 Fragments arranged by size: 535 373 168 25 BanII G_rGCy'C Cuts at: 0 239 502 524 634 791 801 1096 1101 Size: 239 263 22 110 157 10 295 5 Fragments arranged by size: 295 263 239 157 110 22 10 5 BbsI GAAGACnn'nnnn_ Cuts at: 0 100 763 1101 Size: 100 663 338 Fragments arranged by size: 663 338 100 BciVI GTATCCnnnnn_n' Cuts at: 0 128 1101 Size: 128 973 BglI GCCn_nnn'nGGC Cuts at: 0 334 421 519 895 1101 Size: 334 87 98 376 206 Fragments arranged by size: 376 334 206 98 87 BglII A'GATC_T Cuts at: 0 740 1101 Size: 740 361 BlpI GC'TnA_GC Cuts at: 0 49 584 1101 Size: 49 535 517 Fragments arranged by size: 535 517 49 Bme1580I G_kGCm'C Cuts at: 0 28 196 791 1101 Size: 28 168 595 310 Fragments arranged by size: 595 310 168 28 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 660 772 1101 Size: 660 112 329 Fragments arranged by size: 660 329 112 Bpu10I CC'TnA_GC Cuts at: 0 304 496 1101 Size: 304 192 605 Fragments arranged by size: 605 304 192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 922 1101 Size: 922 179 BsaI GGTCTCn'nnnn_ Cuts at: 0 711 1101 Size: 711 390 BsaHI Gr'CG_yC Cuts at: 0 601 1101 Size: 601 500 BsaWI w'CCGG_w Cuts at: 0 361 730 1101 Size: 361 369 371 Fragments arranged by size: 371 369 361 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 135 138 159 360 363 366 390 1101 Size: 135 3 21 201 3 3 24 711 Fragments arranged by size: 711 201 135 24 21 3 3 3 BseYI C'CCAG_C Cuts at: 0 535 1101 Size: 535 566 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 1057 1101 Size: 58 999 44 Fragments arranged by size: 999 58 44 BsiEI CG_ry'CG Cuts at: 0 275 437 548 1101 Size: 275 162 111 553 Fragments arranged by size: 553 275 162 111 BsiHKAI G_wGCw'C Cuts at: 0 1096 1101 Size: 1096 5 BsmI GAATG_Cn' Cuts at: 0 40 1101 Size: 40 1061 Bsp1286I G_dGCh'C Cuts at: 0 28 196 239 502 524 634 791 801 Size: 28 168 43 263 22 110 157 10 Cuts at: 801 1096 1101 Size: 295 5 Fragments arranged by size: 295 263 168 157 110 43 28 22 10 5 BspEI T'CCGG_A Cuts at: 0 730 1101 Size: 730 371 BsrDI GCAATG_nn' Cuts at: 0 23 1101 Size: 23 1078 BsrFI r'CCGG_y Cuts at: 0 133 308 563 1101 Size: 133 175 255 538 Fragments arranged by size: 538 255 175 133 BssSI C'ACGA_G Cuts at: 0 714 1101 Size: 714 387 BstBI TT'CG_AA Cuts at: 0 764 1101 Size: 764 337 BstXI CCAn_nnnn'nTGG Cuts at: 0 324 579 687 1101 Size: 324 255 108 414 Fragments arranged by size: 414 324 255 108 BstYI r'GATC_y Cuts at: 0 676 740 990 1101 Size: 676 64 250 111 Fragments arranged by size: 676 250 111 64 BtgI C'CryG_G Cuts at: 0 227 323 419 431 542 548 557 899 Size: 227 96 96 12 111 6 9 342 Cuts at: 899 1101 Size: 202 Fragments arranged by size: 342 227 202 111 96 96 12 9 6 EaeI y'GGCC_r Cuts at: 0 65 83 224 272 434 470 545 551 Size: 65 18 141 48 162 36 75 6 Cuts at: 551 594 896 902 1101 Size: 43 302 6 199 Fragments arranged by size: 302 199 162 141 75 65 48 43 36 18 6 6 EagI C'GGCC_G Cuts at: 0 272 434 545 1101 Size: 272 162 111 556 Fragments arranged by size: 556 272 162 111 EarI CTCTTCn'nnn_ Cuts at: 0 384 697 956 1101 Size: 384 313 259 145 Fragments arranged by size: 384 313 259 145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 339 378 393 1101 Size: 339 39 15 708 Fragments arranged by size: 708 339 39 15 EcoICRI GAG'CTC Cuts at: 0 1094 1101 Size: 1094 7 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 513 660 772 1101 Size: 235 278 147 112 329 Fragments arranged by size: 329 278 235 147 112 EcoNI CCTnn'n_nnAGG Cuts at: 0 671 1101 Size: 671 430 EcoO109I rG'GnC_Cy Cuts at: 0 787 944 1101 Size: 787 157 157 Fragments arranged by size: 787 157 157 EcoRV GAT'ATC Cuts at: 0 300 1101 Size: 300 801 HaeII r_GCGC'y Cuts at: 0 542 936 1101 Size: 542 394 165 Fragments arranged by size: 542 394 165 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 725 757 816 848 982 1014 1081 1101 Size: 725 32 59 32 134 32 67 20 Fragments arranged by size: 725 134 67 59 32 32 32 20 KpnI G_GTAC'C Cuts at: 0 570 1101 Size: 570 531 MluI A'CGCG_T Cuts at: 0 1076 1101 Size: 1076 25 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 668 1101 Size: 668 433 MscI TGG'CCA Cuts at: 0 898 1101 Size: 898 203 MslI CAynn'nnrTG Cuts at: 0 322 1101 Size: 322 779 MspA1I CmG'CkG Cuts at: 0 102 126 421 433 544 579 1023 1049 Size: 102 24 295 12 111 35 444 26 Cuts at: 1049 1101 Size: 52 Fragments arranged by size: 444 295 111 102 52 35 26 24 12 NaeI GCC'GGC Cuts at: 0 135 310 1101 Size: 135 175 791 Fragments arranged by size: 791 175 135 NgoMIV G'CCGG_C Cuts at: 0 133 308 1101 Size: 133 175 793 Fragments arranged by size: 793 175 133 NotI GC'GGCC_GC Cuts at: 0 434 1101 Size: 434 667 PflMI CCAn_nnn'nTGG Cuts at: 0 611 1101 Size: 611 490 PspOMI G'GGCC_C Cuts at: 0 787 1101 Size: 787 314 PvuII CAG'CTG Cuts at: 0 1023 1101 Size: 1023 78 SacI G_AGCT'C Cuts at: 0 1096 1101 Size: 1096 5 SacII CC_GC'GG Cuts at: 0 422 434 545 1101 Size: 422 12 111 556 Fragments arranged by size: 556 422 111 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 334 1101 Size: 334 767 SmaI CCC'GGG Cuts at: 0 519 1101 Size: 519 582 SmlI C'TyrA_G Cuts at: 0 937 1101 Size: 937 164 SrfI GCCC'GGGC Cuts at: 0 519 1101 Size: 519 582 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 211 1101 Size: 211 890 XmaI C'CCGG_G Cuts at: 0 517 1101 Size: 517 584 XmnI GAAnn'nnTTC Cuts at: 0 760 1101 Size: 760 341 Enzymes that do cut: Acc65I AcuI AflIII AhdI ApaI AvaI BamHI BanI BanII BbsI BciVI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrFI BssSI BstBI BstXI BstYI BtgI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KpnI MluI MmeI MscI MslI MspA1I NaeI NgoMIV NotI PflMI PspOMI PvuII SacI SacII SfiI SmaI SmlI SrfI TaqII XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaXI BsiWI BsmBI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI NarI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI