################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MaeII MamI MluI MluNI MroI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 1459 GTAGAC GT^[AC][GT]AC AccII 139 CGCG CG^CG Alw44I 1604 GTGCAC G^TGCAC AlwI 176 GATCC GGATC(4/5) ApaLI 1604 GTGCAC G^TGCAC Asp700I 27 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BciVI 1312 GGATAC GTATCC(6/5) BsmBI 99 GAGACG CGTCTC(1/5) BsmI 1431 GCATTC GAATGC(1/-1) BspHI 2291 TCATGA T^CATGA BspMI 191 GCAGGT ACCTGC(4/8) BsrBI 1093 GAGCGG CCGCTC(-3/-3) BsrFI 516 GCCGGT [GA]^CCGG[CT] BstAPI 2145 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 139 CGCG CG^CG CfoI 915 GCGC GCG^C Cfr10I 516 GCCGGT [GA]^CCGG[CT] DraI 1329 TTTAAA TTT^AAA EcoRV 258 GATATC GAT^ATC EcoT22I 1429 ATGCAT ATGCA^T FauI 54 GCGGG CCCGC(4/6) HgaI 10 GACGC GACGC(5/10) HhaI 915 GCGC GCG^C HinP1I 915 GCGC G^CGC HincII 2158 GTTGAC GT[CT]^[GA]AC HindII 2158 GTTGAC GT[CT]^[GA]AC MfeI 293 CAATTG C^AATTG MunI 293 CAATTG C^AATTG MvnI 139 CGCG CG^CG NsiI 1429 ATGCAT ATGCA^T PshAI 1969 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2047 CTGCAG CTGCA^G RcaI 2291 TCATGA T^CATGA SmaI 1129 CCCGGG CCC^GGG XmaCI 1129 CCCGGG C^CCGGG XmaI 1129 CCCGGG C^CCGGG XmnI 27 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 329 GTAC GT^AC 2244 GTAC AspHI 671 GTGCTC G[AT]GC[AT]^C 1604 GTGCAC AvaII 360 GGACC G^G[AT]CC 609 GGTCC BbsI 820 GAAGAC GAAGAC(2/6) 2124 GAAGAC BfaI 188 CTAG C^TAG 215 CTAG BglII 1680 AGATCT A^GATCT 2109 AGATCT BpuAI 820 GAAGAC GAAGAC(2/6) 2124 GAAGAC BsiHKAI 671 GTGCTC G[AT]GC[AT]^C 1604 GTGCAC BsrDI 1070 GCAATG GCAATG(2/0) 1075 GCAATG EcoNI 702 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1941 CCTGGAGGAGG EcoRI 1398 GAATTC G^AATTC 2232 GAATTC EcoT14I 241 CCAAGG C^C[AT][AT]GG 717 CCAAGG MaeI 188 CTAG C^TAG 215 CTAG NspI 59 ACATGC [GA]CATG^[CT] 1767 GCATGT RsaI 329 GTAC GT^AC 2244 GTAC SexAI 2246 ACCTGGT A^CC[AT]GGT 2309 ACCAGGT SmlI 734 CTCAAG C^T[CT][GA]AG 1918 CTCAAG StyI 241 CCAAGG C^C[AT][AT]GG 717 CCAAGG TaqI 967 TCGA T^CGA 1477 TCGA TthHB8I 967 TCGA T^CGA 1477 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AluI AlwNI AocI ApoI AvaI BanI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII EarI Eco81I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII