(Circular) (Six-base) MAPSORT of: pFN21AE4230.seq Check: 6602 from: 1 to: 7129 pFN21AE4230 7129 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4939 278 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3465 4472 1937 Size: 1528 1007 4594 Fragments arranged by size: 4594 1528 1007 Acc65I G'GTAC_C Cuts at: 4450 4450 Size: 7129 AclI AA'CG_TT Cuts at: 1563 5256 5629 1563 Size: 3693 373 3063 Fragments arranged by size: 3693 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2023 2603 2898 2916 3333 3471 5192 6576 Size: 391 580 295 18 417 138 1721 1384 Cuts at: 6576 6844 1632 Size: 268 1917 Fragments arranged by size: 1917 1721 1384 580 417 391 295 268 138 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6932 AflIII A'CryG_T Cuts at: 1203 6028 7005 1203 Size: 4825 977 1327 Fragments arranged by size: 4825 1327 977 AgeI A'CCGG_T Cuts at: 5960 5960 Size: 7129 AhdI GACnn_n'nnGTC Cuts at: 5858 5858 Size: 7129 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2526 4302 1184 Size: 1342 1776 4011 Fragments arranged by size: 4011 1776 1342 AlwNI CAG_nnn'CTG Cuts at: 2056 3869 3914 4872 6444 2056 Size: 1813 45 958 1572 2741 Fragments arranged by size: 2741 1813 1572 958 45 ApaLI G'TGCA_C Cuts at: 3609 5186 6342 3609 Size: 1577 1156 4396 Fragments arranged by size: 4396 1577 1156 ApoI r'AATT_y Cuts at: 1493 2995 3349 3403 3617 4237 4375 4438 4664 Size: 1502 354 54 214 620 138 63 226 Cuts at: 4664 4816 6925 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1502 620 354 226 214 152 138 63 54 AseI AT'TA_AT Cuts at: 160 1032 5683 160 Size: 872 4651 1606 Fragments arranged by size: 4651 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 AvaI C'yCGr_G Cuts at: 1870 1943 2467 2671 2758 3134 4454 1870 Size: 73 524 204 87 376 1320 4545 Fragments arranged by size: 4545 1320 524 376 204 87 73 BamHI G'GATC_C Cuts at: 4459 4826 4459 Size: 367 6762 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2518 3299 4121 4450 5905 Size: 325 217 627 731 781 822 329 1455 Cuts at: 5905 618 Size: 1842 Fragments arranged by size: 1842 1455 822 781 731 627 329 325 217 BanII G_rGCy'C Cuts at: 1391 3051 3203 3231 3673 4448 1391 Size: 1660 152 28 442 775 4072 Fragments arranged by size: 4072 1660 775 442 152 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2832 4136 928 Size: 971 933 1304 3921 Fragments arranged by size: 3921 1304 971 933 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5320 5354 1903 Size: 34 3383 34 3678 Fragments arranged by size: 3678 3383 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3311 5021 6231 3311 Size: 1710 1210 4209 Fragments arranged by size: 4209 1710 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7129 BfrBI ATG'CAT Cuts at: 3436 3436 Size: 7129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5740 136 Size: 107 122 71 631 605 4068 1525 Fragments arranged by size: 4068 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3685 4114 7124 3685 Size: 429 3010 3690 Fragments arranged by size: 3690 3010 429 BlpI GC'TnA_GC Cuts at: 4546 4546 Size: 7129 Bme1580I G_kGCm'C Cuts at: 1790 3613 5190 6346 1790 Size: 1823 1577 1156 2573 Fragments arranged by size: 2573 1823 1577 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2884 3045 3593 Size: 416 53 83 399 24 1467 161 548 Cuts at: 3593 4860 5818 442 Size: 1267 958 1753 Fragments arranged by size: 1753 1467 1267 958 548 416 399 161 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7129 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3532 3973 3532 Size: 441 6688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2367 3968 4046 5789 1239 Size: 1128 1601 78 1743 2579 Fragments arranged by size: 2579 1743 1601 1128 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2724 3908 5254 6119 6417 2724 Size: 1184 1346 865 298 3436 Fragments arranged by size: 3436 1346 1184 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2118 2661 3789 5792 882 Size: 617 619 543 1128 2003 2219 Fragments arranged by size: 2219 2003 1128 619 617 543 BsaAI yAC'GTr Cuts at: 493 1204 4920 493 Size: 711 3716 2702 Fragments arranged by size: 3716 2702 711 BsaBI GATnn'nnATC Cuts at: 7123 7123 Size: 7129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4936 5318 5953 275 Size: 53 83 186 936 3403 382 635 1451 Fragments arranged by size: 3403 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5562 5960 6234 6381 6792 5562 Size: 398 274 147 411 5899 Fragments arranged by size: 5899 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3259 3289 1178 Size: 30 165 30 1856 30 5018 Fragments arranged by size: 5018 1856 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2449 3727 3965 3977 3980 1186 Size: 1054 209 1278 238 12 3 4335 Fragments arranged by size: 4335 1278 1054 238 209 12 3 BseYI C'CCAG_C Cuts at: 2369 3110 6332 6828 2369 Size: 741 3222 496 2670 Fragments arranged by size: 3222 2670 741 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6556 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4579 5340 6368 1540 Size: 275 186 2578 761 1028 2301 Fragments arranged by size: 2578 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2680 3613 5190 5275 6346 2680 Size: 933 1577 85 1071 3463 Fragments arranged by size: 3463 1577 1071 933 85 BsmI GAATG_Cn' Cuts at: 3436 4640 4733 3436 Size: 1204 93 5832 Fragments arranged by size: 5832 1204 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2098 2098 Size: 7129 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2680 3051 3203 3231 3613 3673 4448 Size: 399 890 371 152 28 382 60 775 Cuts at: 4448 5190 5275 6346 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 890 775 742 399 382 371 152 85 60 28 BspEI T'CCGG_A Cuts at: 6792 6792 Size: 7129 BspHI T'CATG_A Cuts at: 4296 5018 6748 4296 Size: 722 1730 4677 Fragments arranged by size: 4677 1730 722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 4484 844 Size: 1343 2297 3489 Fragments arranged by size: 3489 2297 1343 BsrBI CCG'CTC Cuts at: 1394 1823 3100 5016 1394 Size: 429 1277 1916 3507 Fragments arranged by size: 3507 1916 1277 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3082 3087 5624 5798 65 Size: 1183 1834 5 2537 174 1396 Fragments arranged by size: 2537 1834 1396 1183 174 5 BsrFI r'CCGG_y Cuts at: 1700 1810 2521 5773 5960 1700 Size: 110 711 3252 187 2869 Fragments arranged by size: 3252 2869 711 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7129 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7129 BssSI C'ACGA_G Cuts at: 5189 6201 5189 Size: 1012 6117 BstAPI GCAn_nnn'nTGC Cuts at: 794 4156 794 Size: 3362 3767 BstBI TT'CG_AA Cuts at: 5933 5933 Size: 7129 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7129 BstYI r'GATC_y Cuts at: 1972 2180 3685 4114 4459 4826 5211 5228 6680 Size: 208 1505 429 345 367 385 17 1452 Cuts at: 6680 6766 6778 7124 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1505 1452 429 385 367 346 345 208 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2432 2979 3663 2432 Size: 547 684 5898 Fragments arranged by size: 5898 684 547 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6577 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2058 2883 487 Size: 683 56 832 825 4733 Fragments arranged by size: 4733 832 825 683 56 BtsI GCAGTG_nn' Cuts at: 2510 3187 3825 4645 5439 5459 2510 Size: 677 638 820 794 20 4180 Fragments arranged by size: 4180 820 794 677 638 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7129 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7094 DraI TTT'AAA Cuts at: 3336 4433 3336 Size: 1097 6032 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6136 809 Size: 507 4820 1802 Fragments arranged by size: 4820 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4576 5465 7094 Size: 54 1425 50 275 18 2746 889 1629 Cuts at: 7094 8 Size: 43 Fragments arranged by size: 2746 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4576 1537 Size: 275 2764 4090 Fragments arranged by size: 4090 2764 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2116 2579 2854 2874 5059 1353 Size: 763 463 275 20 2185 3423 Fragments arranged by size: 3423 2185 763 463 275 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5716 6090 6236 387 Size: 1064 138 4127 374 146 1280 Fragments arranged by size: 4127 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2367 2603 2898 2916 3333 3471 Size: 393 391 344 236 295 18 417 138 Cuts at: 3471 3968 4046 5192 5789 6576 6844 1239 Size: 497 78 1146 597 787 268 1524 Fragments arranged by size: 1524 1146 787 597 497 417 393 391 344 295 268 236 138 78 18 EcoNI CCTnn'n_nnAGG Cuts at: 2711 3950 2711 Size: 1239 5890 EcoRI G'AATT_C Cuts at: 3403 4237 4438 4816 3403 Size: 834 201 378 5716 Fragments arranged by size: 5716 834 378 201 EcoRV GAT'ATC Cuts at: 2265 2265 Size: 7129 FspI TGC'GCA Cuts at: 5635 5635 Size: 7129 HaeII r_GCGC'y Cuts at: 1678 6276 1678 Size: 4598 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 2430 2462 2578 Size: 32 135 32 722 32 104 32 116 Cuts at: 2578 2610 3137 3169 3206 3238 4319 4351 5770 Size: 32 527 32 37 32 1081 32 1419 Cuts at: 5770 5802 5844 5876 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1419 1081 722 527 135 116 104 42 37 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4165 4473 4719 669 Size: 988 281 2227 308 246 3079 Fragments arranged by size: 3079 2227 988 308 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7129 HpaI GTT'AAC Cuts at: 4719 4719 Size: 7129 KpnI G_GTAC'C Cuts at: 4454 4454 Size: 7129 MfeI C'AATT_G Cuts at: 2298 4728 2298 Size: 2430 4699 MluI A'CGCG_T Cuts at: 7005 7005 Size: 7129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6243 6427 1305 Size: 4938 184 2007 Fragments arranged by size: 4938 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5307 Fragments arranged by size: 5307 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2275 3771 4272 5087 5446 5605 518 Size: 648 1109 1496 501 815 359 159 2042 Fragments arranged by size: 2042 1496 1109 815 648 501 359 159 MspA1I CmG'CkG Cuts at: 1606 4545 5222 6370 6615 1606 Size: 2939 677 1148 245 2120 Fragments arranged by size: 2939 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6577 NdeI CA'TA_TG Cuts at: 387 387 Size: 7129 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7129 NotI GC'GGCC_GC Cuts at: 4576 4576 Size: 7129 NsiI A_TGCA'T Cuts at: 3438 3438 Size: 7129 NspI r_CATG'y Cuts at: 1132 1229 2068 3776 4487 6032 1132 Size: 97 839 1708 711 1545 2229 Fragments arranged by size: 2229 1708 1545 839 711 97 PasI CC'CwG_GG Cuts at: 1348 2529 1348 Size: 1181 5948 PciI A'CATG_T Cuts at: 6028 6028 Size: 7129 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7129 PfoI T'CCnGG_A Cuts at: 3945 4023 3945 Size: 78 7051 PmeI GTTT'AAAC Cuts at: 4433 4433 Size: 7129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5198 5230 6711 6743 5198 Size: 32 1481 32 5584 Fragments arranged by size: 5584 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3978 1613 Size: 2365 4764 PsiI TTA'TAA Cuts at: 4699 4699 Size: 7129 PstI C_TGCA'G Cuts at: 830 4056 4481 830 Size: 3226 425 3478 Fragments arranged by size: 3478 3226 425 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7129 SalI G'TCGA_C Cuts at: 1936 4471 1936 Size: 2535 4594 SbfI CC_TGCA'GG Cuts at: 4481 4481 Size: 7129 ScaI AGT'ACT Cuts at: 5377 7089 5377 Size: 1712 5417 SexAI A'CCwGG_T Cuts at: 4251 4314 4251 Size: 63 7066 SfcI C'TryA_G Cuts at: 826 1045 2784 3159 4052 4477 5612 6293 6484 Size: 219 1739 375 893 425 1135 681 191 Cuts at: 6484 826 Size: 1471 Fragments arranged by size: 1739 1471 1135 893 681 425 375 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7129 SmaI CCC'GGG Cuts at: 1872 3136 4456 1872 Size: 1264 1320 4545 Fragments arranged by size: 4545 1320 1264 SmlI C'TyrA_G Cuts at: 820 1017 1943 2739 3923 5233 6134 6396 820 Size: 197 926 796 1184 1310 901 262 1553 Fragments arranged by size: 1553 1310 1184 926 901 796 262 197 SnaBI TAC'GTA Cuts at: 493 4920 493 Size: 4427 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7129 SphI G_CATG'C Cuts at: 1132 4487 1132 Size: 3355 3774 SspI AAT'ATT Cuts at: 5 52 5053 5 Size: 47 5001 2081 Fragments arranged by size: 5001 2081 47 StuI AGG'CCT Cuts at: 1517 2562 2717 3295 4155 1517 Size: 1045 155 578 860 4491 Fragments arranged by size: 4491 1045 860 578 155 StyI C'CwwG_G Cuts at: 513 1065 2246 2722 4568 513 Size: 552 1181 476 1846 3074 Fragments arranged by size: 3074 1846 1181 552 476 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2791 3317 4160 5156 5173 5326 5511 Size: 1255 1211 526 843 996 17 153 185 Cuts at: 5511 5956 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1211 996 843 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5375 7087 96 Size: 274 80 33 51 1443 3398 1712 138 Fragments arranged by size: 3398 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7089 7121 7089 Size: 32 7097 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7129 XbaI T'CTAG_A Cuts at: 4465 4465 Size: 7129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7129 XmaI C'CCGG_G Cuts at: 1870 3134 4454 1870 Size: 1264 1320 4545 Fragments arranged by size: 4545 1320 1264 XmnI GAAnn'nnTTC Cuts at: 2036 5258 2036 Size: 3222 3907 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4937 276 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI