Restriction map for
pFN21AE2110
(Circular) (Six-base) MAPSORT of: pFN21AE2110.seq Check: 6795 from: 1 to: 7423
pFN21AE2110 7423 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5233 278
Size: 53 83 186 936 3697 2468
Fragments arranged by size:
3697 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4766 1937
Size: 2829 4594
Acc65I G'GTAC_C
Cuts at: 4744 4744
Size: 7423
AclI AA'CG_TT
Cuts at: 1563 5550 5923 1563
Size: 3987 373 3063
Fragments arranged by size:
3987 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2461 2983 4467 5486 6870 7138 1632
Size: 829 522 1484 1019 1384 268 1917
Fragments arranged by size:
1917 1484 1384 1019 829 522 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7226
AflIII A'CryG_T
Cuts at: 1203 6322 7299 1203
Size: 5119 977 1327
Fragments arranged by size:
5119 1327 977
AgeI A'CCGG_T
Cuts at: 6254 6254
Size: 7423
AhdI GACnn_n'nnGTC
Cuts at: 6152 6152
Size: 7423
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4183 1184
Size: 2999 4424
AlwNI CAG_nnn'CTG
Cuts at: 2561 3806 5166 6738 2561
Size: 1245 1360 1572 3246
Fragments arranged by size:
3246 1572 1360 1245
ApaLI G'TGCA_C
Cuts at: 3205 5480 6636 3205
Size: 2275 1156 3992
Fragments arranged by size:
3992 2275 1156
ApoI r'AATT_y
Cuts at: 1493 2365 2434 2567 2723 2852 4264 4685 4732
Size: 872 69 133 156 129 1412 421 47
Cuts at: 4732 4958 5110 7219 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 872 421 226 156 152
133 129 69 47
AseI AT'TA_AT
Cuts at: 160 1032 5977 160
Size: 872 4945 1606
Fragments arranged by size:
4945 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7423
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 4295 4463 4748 1870
Size: 73 119 2233 168 285 4545
Fragments arranged by size:
4545 2233 285 168 119 73
AvrII C'CTAG_G
Cuts at: 2444 3908 2444
Size: 1464 5959
BamHI G'GATC_C
Cuts at: 4753 5120 4753
Size: 367 7056
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3428 3490 4225 4744 6199
Size: 325 217 627 1641 62 735 519 1455
Cuts at: 6199 618
Size: 1842
Fragments arranged by size:
1842 1641 1455 735 627 519 325 217
62
BanII G_rGCy'C
Cuts at: 1391 2030 2063 2088 3131 3189 3355 3894 4742
Size: 639 33 25 1043 58 166 539 848
Cuts at: 4742 1391
Size: 4072
Fragments arranged by size:
4072 1043 848 639 539 166 58 33
25
BbeI G_GCGC'C
Cuts at: 4229 4229
Size: 7423
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6452
BbvCI CC'TCA_GC
Cuts at: 2132 4569 2132
Size: 2437 4986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5614 5648 1903
Size: 34 3677 34 3678
Fragments arranged by size:
3678 3677 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3928 4132 5315 6525 3928
Size: 204 1183 1210 4826
Fragments arranged by size:
4826 1210 1183 204
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7423
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4129 4358 6034
Size: 107 122 71 631 605 2457 229 1676
Cuts at: 6034 136
Size: 1525
Fragments arranged by size:
2457 1676 1525 631 605 229 122 107
71
BglII A'GATC_T
Cuts at: 3625 7418 3625
Size: 3793 3630
BlpI GC'TnA_GC
Cuts at: 2888 4840 2888
Size: 1952 5471
Bme1580I G_kGCm'C
Cuts at: 1790 3209 5484 6640 1790
Size: 1419 2275 1156 2573
Fragments arranged by size:
2573 2275 1419 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7423
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5154 6112 442
Size: 416 53 83 399 24 3737 958 1753
Fragments arranged by size:
3737 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7423
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2913 3145 2913
Size: 232 7191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2681 2831 2933 3630 3709 4561 6083 1239
Size: 1442 150 102 697 79 852 1522 2579
Fragments arranged by size:
2579 1522 1442 852 697 150 102 79
Bpu10I CC'TnA_GC
Cuts at: 2132 2768 4569 2132
Size: 636 1801 4986
Fragments arranged by size:
4986 1801 636
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3520 5548 6413 6711 3520
Size: 2028 865 298 4232
Fragments arranged by size:
4232 2028 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6086 882
Size: 617 4587 2219
Fragments arranged by size:
4587 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4194 5214 493
Size: 711 2990 1020 2702
Fragments arranged by size:
2990 2702 1020 711
BsaBI GATnn'nnATC
Cuts at: 4425 7417 4425
Size: 2992 4431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 3691 4226 5230
Size: 53 83 186 936 564 1594 535 1004
Cuts at: 5230 5612 6247 275
Size: 382 635 1451
Fragments arranged by size:
1594 1451 1004 936 635 564 535 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5856 6254 6528 6675 7086 5856
Size: 398 274 147 411 6193
Fragments arranged by size:
6193 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3707 3737 1178
Size: 30 165 30 2304 30 4864
Fragments arranged by size:
4864 2304 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 4313 4387 1186
Size: 1258 1869 74 4222
Fragments arranged by size:
4222 1869 1258 74
BseYI C'CCAG_C
Cuts at: 6626 7122 6626
Size: 496 6927
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6850
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3446 4353 4873 5634 6662 1540
Size: 275 186 1445 907 520 761 1028 2301
Fragments arranged by size:
2301 1445 1028 907 761 520 275 186
BsiHKAI G_wGCw'C
Cuts at: 2030 3209 3355 5484 5569 6640 2030
Size: 1179 146 2129 85 1071 2813
Fragments arranged by size:
2813 2129 1179 1071 146 85
BsmI GAATG_Cn'
Cuts at: 4934 5027 4934
Size: 93 7330
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2063 2088 3131 3189 3209 3355
Size: 399 240 33 25 1043 58 20 146
Cuts at: 3355 3894 4742 5484 5569 6640 1391
Size: 539 848 742 85 1071 2174
Fragments arranged by size:
2174 1071 1043 848 742 539 399 240
146 85 58 33 25 20
BspEI T'CCGG_A
Cuts at: 7086 7086
Size: 7423
BspHI T'CATG_A
Cuts at: 2088 3408 4596 5312 7042 2088
Size: 1320 1188 716 1730 2469
Fragments arranged by size:
2469 1730 1320 1188 716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 2705 4778 844
Size: 1427 434 2073 3489
Fragments arranged by size:
3489 2073 1427 434
BsrBI CCG'CTC
Cuts at: 1394 1823 5310 1394
Size: 429 3487 3507
Fragments arranged by size:
3507 3487 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2707 3599 3872 4082 4209 4641 5918
Size: 1183 1459 892 273 210 127 432 1277
Cuts at: 5918 6092 65
Size: 174 1396
Fragments arranged by size:
1459 1396 1277 1183 892 432 273 210
174 127
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 3651 3852 4348 6067 6254 1700
Size: 110 199 1642 201 496 1719 187 2869
Fragments arranged by size:
2869 1719 1642 496 201 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 3704 96
Size: 3608 3815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7423
BssSI C'ACGA_G
Cuts at: 4155 5483 6495 4155
Size: 1328 1012 5083
Fragments arranged by size:
5083 1328 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2561 4280 794
Size: 1767 1719 3937
Fragments arranged by size:
3937 1767 1719
BstBI TT'CG_AA
Cuts at: 6227 6227
Size: 7423
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7423
BstYI r'GATC_y
Cuts at: 1972 3298 3625 4753 5120 5505 5522 6974 7060
Size: 1326 327 1128 367 385 17 1452 86
Cuts at: 7060 7072 7418 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1326 1128 385 367 346 327
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2667 3462 4487 513
Size: 552 939 663 795 1025 3449
Fragments arranged by size:
3449 1025 939 795 663 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6684
Fragments arranged by size:
6684 683 56
BtsI GCAGTG_nn'
Cuts at: 4939 5733 5753 4939
Size: 794 20 6609
Fragments arranged by size:
6609 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7423
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7388
DraI TTT'AAA
Cuts at: 4727 4727
Size: 7423
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6430 809
Size: 507 5114 1802
Fragments arranged by size:
5114 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3443 3653
Size: 54 1425 50 275 18 177 1436 210
Cuts at: 3653 3782 4269 4350 4665 4870 5759 7388 8
Size: 129 487 81 315 205 889 1629 43
Fragments arranged by size:
1629 1436 1425 889 487 315 275 210
205 177 129 81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3443 4350 4870 1537
Size: 275 1631 907 520 4090
Fragments arranged by size:
4090 1631 907 520 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 2405 2606 2981 3410 3832 4050 4083
Size: 665 387 201 375 429 422 218 33
Cuts at: 4083 4120 5353 1353
Size: 37 1233 3423
Fragments arranged by size:
3423 1233 665 429 422 387 375 218
201 37 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6010 6384 6530 387
Size: 1064 138 4421 374 146 1280
Fragments arranged by size:
4421 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2028 3353 2028
Size: 1325 6098
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2461 2681 2831 2933 2983 3630 3709
Size: 393 829 220 150 102 50 647 79
Cuts at: 3709 4467 4561 5486 6083 6870 7138 1239
Size: 758 94 925 597 787 268 1524
Fragments arranged by size:
1524 925 829 787 758 647 597 393
268 220 150 102 94 79 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2402 4117 2402
Size: 1715 5708
EcoRI G'AATT_C
Cuts at: 2434 4732 5110 2434
Size: 2298 378 4747
Fragments arranged by size:
4747 2298 378
EcoRV GAT'ATC
Cuts at: 4381 4381
Size: 7423
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 3497 2116
Size: 1381 6042
FspI TGC'GCA
Cuts at: 5929 5929
Size: 7423
HaeII r_GCGC'y
Cuts at: 1678 2234 4229 6570 1678
Size: 556 1995 2341 2531
Fragments arranged by size:
2531 2341 1995 556
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2944 2976 3707 3739 6064
Size: 32 135 32 1372 32 731 32 2325
Cuts at: 6064 6096 6138 6170 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2325 1372 731 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4767 5013 669
Size: 988 281 2829 246 3079
Fragments arranged by size:
3079 2829 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2580 3474 4632 748
Size: 1832 894 1158 3539
Fragments arranged by size:
3539 1832 1158 894
HpaI GTT'AAC
Cuts at: 5013 5013
Size: 7423
KasI G'GCGC_C
Cuts at: 4225 4225
Size: 7423
KpnI G_GTAC'C
Cuts at: 4748 4748
Size: 7423
MfeI C'AATT_G
Cuts at: 5022 5022
Size: 7423
MluI A'CGCG_T
Cuts at: 7299 7299
Size: 7423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3163 3646 6537 6721 1305
Size: 1858 483 2891 184 2007
Fragments arranged by size:
2891 2007 1858 483 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4667 10
Size: 54 1425 343 2835 2766
Fragments arranged by size:
2835 2766 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3947 4159 4661 5381 5740 5899 518
Size: 648 2781 212 502 720 359 159 2042
Fragments arranged by size:
2781 2042 720 648 502 359 212 159
MspA1I CmG'CkG
Cuts at: 1606 2049 4489 4521 4839 5516 6664 6909 1606
Size: 443 2440 32 318 677 1148 245 2120
Fragments arranged by size:
2440 2120 1148 677 443 318 245 32
NaeI GCC'GGC
Cuts at: 2011 4350 2011
Size: 2339 5084
NarI GG'CG_CC
Cuts at: 4226 4226
Size: 7423
NcoI C'CATG_G
Cuts at: 513 1065 2004 2667 3462 513
Size: 552 939 663 795 4474
Fragments arranged by size:
4474 939 795 663 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7423
NgoMIV G'CCGG_C
Cuts at: 2009 4348 2009
Size: 2339 5084
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7423
NotI GC'GGCC_GC
Cuts at: 4870 4870
Size: 7423
NspI r_CATG'y
Cuts at: 1132 1229 2488 4164 4781 6326 1132
Size: 97 1259 1676 617 1545 2229
Fragments arranged by size:
2229 1676 1545 1259 617 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7423
PciI A'CATG_T
Cuts at: 6322 6322
Size: 7423
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2488 1108
Size: 1380 6043
PfoI T'CCnGG_A
Cuts at: 4680 4680
Size: 7423
PmeI GTTT'AAAC
Cuts at: 4727 4727
Size: 7423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3513 3545 5492 5524 7005 7037 3513
Size: 32 1947 32 1481 32 3899
Fragments arranged by size:
3899 1947 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2600 1613
Size: 987 6436
PsiI TTA'TAA
Cuts at: 4993 4993
Size: 7423
PstI C_TGCA'G
Cuts at: 830 4775 830
Size: 3945 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7423
PvuII CAG'CTG
Cuts at: 4521 4521
Size: 7423
SacI G_AGCT'C
Cuts at: 2030 3355 2030
Size: 1325 6098
SacII CC_GC'GG
Cuts at: 4490 4490
Size: 7423
SalI G'TCGA_C
Cuts at: 1936 4765 1936
Size: 2829 4594
SapI GCTCTTCn'nnn_
Cuts at: 2018 2606 2018
Size: 588 6835
SbfI CC_TGCA'GG
Cuts at: 4775 4775
Size: 7423
ScaI AGT'ACT
Cuts at: 5671 7383 5671
Size: 1712 5711
SfcI C'TryA_G
Cuts at: 826 1045 4771 5906 6587 6778 826
Size: 219 3726 1135 681 191 1471
Fragments arranged by size:
3726 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4129 4358 4129
Size: 229 7194
SfoI GGC'GCC
Cuts at: 4227 4227
Size: 7423
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7423
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7423
SmaI CCC'GGG
Cuts at: 1872 4750 1872
Size: 2878 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3535 5527 6428 6690 820
Size: 197 926 1592 1992 901 262 1553
Fragments arranged by size:
1992 1592 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4194 5214 493
Size: 3701 1020 2702
Fragments arranged by size:
3701 2702 1020
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7423
SphI G_CATG'C
Cuts at: 1132 4781 1132
Size: 3649 3774
SspI AAT'ATT
Cuts at: 5 52 4213 5347 5
Size: 47 4161 1134 2081
Fragments arranged by size:
4161 2081 1134 47
StuI AGG'CCT
Cuts at: 1517 4123 4471 1517
Size: 2606 348 4469
Fragments arranged by size:
4469 2606 348
StyI C'CwwG_G
Cuts at: 513 1065 2004 2444 2606 2667 3432 3462 3760
Size: 552 939 440 162 61 765 30 298
Cuts at: 3760 3908 4862 513
Size: 148 954 3074
Fragments arranged by size:
3074 954 939 765 552 440 298 162
148 61 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5450 5467 5620 5805 6250 325
Size: 1255 3870 17 153 185 445 1498
Fragments arranged by size:
3870 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2894 3704 4101
Size: 274 80 33 51 1443 917 810 397
Cuts at: 4101 5669 7381 96
Size: 1568 1712 138
Fragments arranged by size:
1712 1568 1443 917 810 397 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7383 7415 7383
Size: 32 7391
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7423
XbaI T'CTAG_A
Cuts at: 4759 4759
Size: 7423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4027 4286 4027
Size: 259 7164
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7423
XmaI C'CCGG_G
Cuts at: 1870 4748 1870
Size: 2878 4545
XmnI GAAnn'nnTTC
Cuts at: 3559 5552 3559
Size: 1993 5430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5231 276
Size: 53 83 186 936 3697 2468
Fragments arranged by size:
3697 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SanDI SexAI SrfI SwaI