################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AccIII AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmI Bsp1407I BspEI BspHI BspMI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KspI MluI MluNI MroI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PvuI RcaI RsrII SacI SacII SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 772 GGTACC G^GTACC AccII 22 CGCG CG^CG AclI 436 AACGTT AA^CGTT AflIII 2513 ACATGT A^C[GA][CT]GT AocI 241 CCTGAGG CC^T[ACGT]AGG AseI 951 ATTAAT AT^TAAT AsnI 951 ATTAAT AT^TAAT Asp718I 772 GGTACC G^GTACC BbsI 1030 GAAGAC GAAGAC(2/6) BbvCI 1195 CCTCAGC CCTCAGC(-5/-2) BciVI 1742 GTATCC GTATCC(6/5) BclI 489 TGATCA T^GATCA BglII 2093 AGATCT A^GATCT BpuAI 1030 GAAGAC GAAGAC(2/6) BsiEI 136 CGGCCG CG[GA][CT]^CG BsmBI 2632 CGTCTC CGTCTC(1/5) BspDI 2139 ATCGAT AT^CGAT BspLU11I 2513 ACATGT A^CATGT BsrBI 565 GAGCGG CCGCTC(-3/-3) BssSI 2584 CTCGTG CACGAG(-5/-1) BstUI 22 CGCG CG^CG BstYI 2093 AGATCT [GA]^GATC[CT] Bsu36I 241 CCTGAGG CC^T[ACGT]AGG ClaI 2139 ATCGAT AT^CGAT DraIII 1169 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1604 CCATGG C^C[GA][CT]GG EagI 136 CGGCCG C^GGCCG EclXI 136 CGGCCG C^GGCCG Eco52I 136 CGGCCG C^GGCCG Eco81I 241 CCTGAGG CC^T[ACGT]AGG EcoNI 1911 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1312 GAATTC G^AATTC EcoT14I 1604 CCATGG C^C[AT][AT]GG FauI 38 CCCGC CCCGC(4/6) FbaI 489 TGATCA T^GATCA KpnI 772 GGTACC GGTAC^C MfeI 962 CAATTG C^AATTG MflI 2093 AGATCT [GA]^GATC[CT] MunI 962 CAATTG C^AATTG MvnI 22 CGCG CG^CG NcoI 1604 CCATGG C^CATGG PpuMI 75 GGGACCC [GA]G^G[AT]CC[CT] PshAI 1860 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 951 ATTAAT AT^TAAT Psp1406I 436 AACGTT AA^CGTT PvuII 1198 CAGCTG CAG^CTG SapI 2071 GAAGAGC GCTCTTC(1/4) SexAI 302 ACCTGGT A^CC[AT]GGT SmaI 1401 CCCGGG CCC^GGG StyI 1604 CCATGG C^C[AT][AT]GG XhoII 2093 AGATCT [GA]^GATC[CT] XmaCI 1401 CCCGGG C^CCGGG XmaI 1401 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 126 GTCGAC GT^[AC][GT]AC 2226 GTCGAC AlwI 91 GGATC GGATC(4/5) 958 GGATC AvaI 26 CCCGAG C^[CT]CG[GA]G 1401 CCCGGG BanI 66 GGCACC G^G[CT][GA]CC 772 GGTACC BpmI 2342 CTCCAG CTGGAG(16/14) 2089 CTGGAG BsaBI 463 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1737 GATTTGTATC BsoBI 26 CCCGAG C^[CT]CG[GA]G 1401 CCCGGG BsrDI 2198 CATTGC GCAATG(2/0) 1510 GCAATG Cac8I 37 GCCCGC GC[ACGT]^[ACGT]GC 2574 GCTGGC DraII 75 GGGACCC [GA]G^G[ACGT]CC[CT] 1154 AGGGCCT EaeI 136 CGGCCG [CT]^GGCC[GA] 553 CGGCCA EarI 1406 GAAGAG CTCTTC(1/4) 2071 GAAGAG EcoO109I 75 GGGACCC [GA]G^G[ACGT]CC[CT] 1154 AGGGCCT EcoRV 402 GATATC GAT^ATC 1972 GATATC HapII 135 CCGG C^CGG 1402 CCGG HpaII 135 CCGG C^CGG 1402 CCGG Ksp632I 1406 GAAGAG CTCTTC(1/4) 2071 GAAGAG MamI 463 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1737 GATTTGTATC MslI 1611 CATAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2274 CATTGTGGTG MspA1I 139 CCGCTG C[AC]G^C[GT]G 1198 CAGCTG MspI 135 CCGG C^CGG 1402 CCGG NspI 333 GCATGT [GA]CATG^[CT] 2513 ACATGT PflMI 1818 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2568 CCACAAGCTGG SalI 126 GTCGAC G^TCGAC 2226 GTCGAC SmlI 218 CTCAAG C^T[CT][GA]AG 2469 CTTGAG Van91I 1818 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2568 CCACAAGCTGG XcmI 519 CCATATATTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1516 CCACTACATAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I