(Circular) (Six-base) MAPSORT of: pFN21AE9628.seq Check: 7360 from: 1 to: 7351 pFN21AE9628 7351 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5161 278 Size: 53 83 186 936 3625 2468 Fragments arranged by size: 3625 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2132 4232 4694 1937 Size: 195 2100 462 4594 Fragments arranged by size: 4594 2100 462 195 Acc65I G'GTAC_C Cuts at: 2777 4672 2777 Size: 1895 5456 AclI AA'CG_TT Cuts at: 1563 2442 5478 5851 1563 Size: 879 3036 373 3063 Fragments arranged by size: 3063 3036 879 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3054 5414 6798 7066 1632 Size: 1422 2360 1384 268 1917 Fragments arranged by size: 2360 1917 1422 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7154 AflIII A'CryG_T Cuts at: 1203 4518 6250 7227 1203 Size: 3315 1732 977 1327 Fragments arranged by size: 3315 1732 1327 977 AgeI A'CCGG_T Cuts at: 6182 6182 Size: 7351 AhdI GACnn_n'nnGTC Cuts at: 6080 6080 Size: 7351 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7351 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2955 2987 2955 Size: 32 7319 AlwNI CAG_nnn'CTG Cuts at: 5094 6666 5094 Size: 1572 5779 ApaLI G'TGCA_C Cuts at: 5408 6564 5408 Size: 1156 6195 ApoI r'AATT_y Cuts at: 1493 2510 3317 3380 4660 4886 5038 7147 1493 Size: 1017 807 63 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 1017 807 226 152 63 AseI AT'TA_AT Cuts at: 160 1032 2957 5905 160 Size: 872 1925 2948 1606 Fragments arranged by size: 2948 1925 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7351 AvaI C'yCGr_G Cuts at: 1870 1943 2031 3406 4676 1870 Size: 73 88 1375 1270 4545 Fragments arranged by size: 4545 1375 1270 88 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2361 2394 3784 3817 2361 Size: 33 1390 33 5895 Fragments arranged by size: 5895 1390 33 33 BamHI G'GATC_C Cuts at: 4681 5048 4681 Size: 367 6984 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2071 2777 4672 6127 618 Size: 325 217 627 284 706 1895 1455 1842 Fragments arranged by size: 1895 1842 1455 706 627 325 284 217 BanII G_rGCy'C Cuts at: 1391 2044 2118 3916 4481 4670 1391 Size: 653 74 1798 565 189 4072 Fragments arranged by size: 4072 1798 653 565 189 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3042 928 Size: 971 1143 5237 Fragments arranged by size: 5237 1143 971 BbvCI CC'TCA_GC Cuts at: 3201 3201 Size: 7351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5542 5576 1903 Size: 34 50 34 3521 34 3678 Fragments arranged by size: 3678 3521 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 5243 6453 3758 Size: 1485 1210 4656 Fragments arranged by size: 4656 1485 1210 BclI T'GATC_A Cuts at: 1548 2494 1548 Size: 946 6405 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5962 136 Size: 107 122 71 631 605 4290 1525 Fragments arranged by size: 4290 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4098 7346 4098 Size: 3248 4103 BlpI GC'TnA_GC Cuts at: 4768 4768 Size: 7351 Bme1580I G_kGCm'C Cuts at: 1790 5412 6568 1790 Size: 3622 1156 2573 Fragments arranged by size: 3622 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2655 1318 Size: 1337 6014 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2156 5082 6040 Size: 416 53 83 399 24 739 2926 958 Cuts at: 6040 442 Size: 1753 Fragments arranged by size: 2926 1753 958 739 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7351 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3932 Size: 7351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4115 4332 6011 1239 Size: 2876 217 1679 2579 Fragments arranged by size: 2876 2579 1679 217 Bpu10I CC'TnA_GC Cuts at: 3201 3201 Size: 7351 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2208 4495 5476 6341 6639 2208 Size: 2287 981 865 298 2920 Fragments arranged by size: 2920 2287 981 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6014 882 Size: 617 4515 2219 Fragments arranged by size: 4515 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5142 493 Size: 711 3938 2702 Fragments arranged by size: 3938 2702 711 BsaBI GATnn'nnATC Cuts at: 2472 3746 7345 2472 Size: 1274 3599 2478 Fragments arranged by size: 3599 2478 1274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5158 5540 6175 275 Size: 53 83 186 936 3625 382 635 1451 Fragments arranged by size: 3625 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5784 6182 6456 6603 7014 5784 Size: 398 274 147 411 6121 Fragments arranged by size: 6121 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4237 4267 1178 Size: 30 165 30 2834 30 4262 Fragments arranged by size: 4262 2834 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2211 2214 2223 2226 3924 1186 Size: 1025 3 9 3 1698 4613 Fragments arranged by size: 4613 1698 1025 9 3 3 BseYI C'CCAG_C Cuts at: 2252 3885 6554 7050 2252 Size: 1633 2669 496 2553 Fragments arranged by size: 2669 2553 1633 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6778 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2144 4801 5562 6590 1540 Size: 275 186 143 2657 761 1028 2301 Fragments arranged by size: 2657 2301 1028 761 275 186 143 BsiHKAI G_wGCw'C Cuts at: 2092 2218 2594 5412 5497 6568 2092 Size: 126 376 2818 85 1071 2875 Fragments arranged by size: 2875 2818 1071 376 126 85 BsmI GAATG_Cn' Cuts at: 4862 4955 4862 Size: 93 7258 BsmBI CGTCTCn'nnnn_ Cuts at: 4643 4643 Size: 7351 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2092 2118 2218 2594 3916 4481 Size: 399 254 48 26 100 376 1322 565 Cuts at: 4481 4670 5412 5497 6568 1391 Size: 189 742 85 1071 2174 Fragments arranged by size: 2174 1322 1071 742 565 399 376 254 189 100 85 48 26 BspEI T'CCGG_A Cuts at: 7014 7014 Size: 7351 BspHI T'CATG_A Cuts at: 5240 6970 5240 Size: 1730 5621 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4706 844 Size: 3862 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2572 5238 1394 Size: 429 749 2666 3507 Fragments arranged by size: 3507 2666 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3522 4202 5846 6020 65 Size: 1183 2274 680 1644 174 1396 Fragments arranged by size: 2274 1644 1396 1183 680 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5995 6182 1700 Size: 110 4185 187 2869 Fragments arranged by size: 4185 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7351 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7351 BssSI C'ACGA_G Cuts at: 4589 5411 6423 4589 Size: 822 1012 5517 Fragments arranged by size: 5517 1012 822 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7351 BstBI TT'CG_AA Cuts at: 6155 6155 Size: 7351 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7351 BstYI r'GATC_y Cuts at: 1972 4098 4681 5048 5433 5450 6902 6988 7000 Size: 2126 583 367 385 17 1452 86 12 Cuts at: 7000 7346 1972 Size: 346 1977 Fragments arranged by size: 2126 1977 1452 583 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2247 2247 Size: 7351 BtgI C'CryG_G Cuts at: 513 1065 3609 513 Size: 552 2544 4255 Fragments arranged by size: 4255 2544 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6612 Fragments arranged by size: 6612 683 56 BtsI GCAGTG_nn' Cuts at: 3701 4867 5661 5681 3701 Size: 1166 794 20 5371 Fragments arranged by size: 5371 1166 794 20 ClaI AT'CG_AT Cuts at: 1554 4145 1554 Size: 2591 4760 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7316 DraI TTT'AAA Cuts at: 4655 4655 Size: 7351 DraIII CAC_nnn'GTG Cuts at: 3179 3179 Size: 7351 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6358 809 Size: 507 5042 1802 Fragments arranged by size: 5042 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2141 2558 4798 Size: 54 1425 50 275 18 311 417 2240 Cuts at: 4798 5687 7316 8 Size: 889 1629 43 Fragments arranged by size: 2240 1629 1425 889 417 311 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2141 4798 1537 Size: 275 329 2657 4090 Fragments arranged by size: 4090 2657 329 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3406 4071 5281 1353 Size: 2053 665 1210 3423 Fragments arranged by size: 3423 2053 1210 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5938 6312 6458 387 Size: 1064 138 4349 374 146 1280 Fragments arranged by size: 4349 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3054 4115 4332 5414 6011 6798 7066 Size: 393 1422 1061 217 1082 597 787 268 Cuts at: 7066 1239 Size: 1524 Fragments arranged by size: 1524 1422 1082 1061 787 597 393 268 217 EcoNI CCTnn'n_nnAGG Cuts at: 3920 3920 Size: 7351 EcoO109I rG'GnC_Cy Cuts at: 2081 3160 2081 Size: 1079 6272 EcoRI G'AATT_C Cuts at: 3317 4660 5038 3317 Size: 1343 378 5630 Fragments arranged by size: 5630 1343 378 EcoRV GAT'ATC Cuts at: 2409 3979 2409 Size: 1570 5781 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4223 4223 Size: 7351 FspI TGC'GCA Cuts at: 5857 5857 Size: 7351 HaeII r_GCGC'y Cuts at: 1678 2270 2524 4559 6498 1678 Size: 592 254 2035 1939 2531 Fragments arranged by size: 2531 2035 1939 592 254 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 2525 2557 3647 Size: 32 135 32 826 32 95 32 1090 Cuts at: 3647 3679 5992 6024 6066 6098 1373 Size: 32 2313 32 42 32 2626 Fragments arranged by size: 2626 2313 1090 826 135 95 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2133 2446 4233 4374 4632 4695 Size: 988 281 195 313 1787 141 258 63 Cuts at: 4695 4941 669 Size: 246 3079 Fragments arranged by size: 3079 1787 988 313 281 258 246 195 141 63 HindIII A'AGCT_T Cuts at: 748 748 Size: 7351 HpaI GTT'AAC Cuts at: 4941 4941 Size: 7351 KpnI G_GTAC'C Cuts at: 2781 4676 2781 Size: 1895 5456 MfeI C'AATT_G Cuts at: 2967 4950 2967 Size: 1983 5368 MluI A'CGCG_T Cuts at: 7227 7227 Size: 7351 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6465 6649 1305 Size: 5160 184 2007 Fragments arranged by size: 5160 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5529 Fragments arranged by size: 5529 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3620 4283 5309 5668 5827 518 Size: 648 2454 663 1026 359 159 2042 Fragments arranged by size: 2454 2042 1026 663 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 3205 4767 5444 6592 6837 1606 Size: 540 1059 1562 677 1148 245 2120 Fragments arranged by size: 2120 1562 1148 1059 677 540 245 NcoI C'CATG_G Cuts at: 513 1065 3609 513 Size: 552 2544 4255 Fragments arranged by size: 4255 2544 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7351 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7351 NotI GC'GGCC_GC Cuts at: 4798 4798 Size: 7351 NspI r_CATG'y Cuts at: 1132 1229 2342 4522 4709 6254 1132 Size: 97 1113 2180 187 1545 2229 Fragments arranged by size: 2229 2180 1545 1113 187 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7351 PciI A'CATG_T Cuts at: 4518 6250 4518 Size: 1732 5619 PflMI CCAn_nnn'nTGG Cuts at: 1108 3829 4579 1108 Size: 2721 750 3880 Fragments arranged by size: 3880 2721 750 PfoI T'CCnGG_A Cuts at: 4348 4348 Size: 7351 PmeI GTTT'AAAC Cuts at: 4655 4655 Size: 7351 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5420 5452 6933 6965 5420 Size: 32 1481 32 5806 Fragments arranged by size: 5806 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2081 2081 Size: 7351 PshAI GACnn'nnGTC Cuts at: 1613 3869 1613 Size: 2256 5095 PsiI TTA'TAA Cuts at: 4449 4527 4921 4449 Size: 78 394 6879 Fragments arranged by size: 6879 394 78 PstI C_TGCA'G Cuts at: 830 2151 2181 3708 4703 830 Size: 1321 30 1527 995 3478 Fragments arranged by size: 3478 1527 1321 995 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7351 PvuII CAG'CTG Cuts at: 3205 3205 Size: 7351 SalI G'TCGA_C Cuts at: 1936 2131 4231 4693 1936 Size: 195 2100 462 4594 Fragments arranged by size: 4594 2100 462 195 SanDI GG'GwC_CC Cuts at: 2081 2081 Size: 7351 SapI GCTCTTCn'nnn_ Cuts at: 4071 4071 Size: 7351 SbfI CC_TGCA'GG Cuts at: 4703 4703 Size: 7351 ScaI AGT'ACT Cuts at: 5599 7311 5599 Size: 1712 5639 SexAI A'CCwGG_T Cuts at: 2307 2307 Size: 7351 SfcI C'TryA_G Cuts at: 826 1045 2147 2177 2998 3085 3544 3590 3704 Size: 219 1102 30 821 87 459 46 114 Cuts at: 3704 4699 5834 6515 6706 826 Size: 995 1135 681 191 1471 Fragments arranged by size: 1471 1135 1102 995 821 681 459 219 191 114 87 46 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7351 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7351 SmaI CCC'GGG Cuts at: 1872 3408 4678 1872 Size: 1536 1270 4545 Fragments arranged by size: 4545 1536 1270 SmlI C'TyrA_G Cuts at: 820 1017 1943 2223 4474 5455 6356 6618 820 Size: 197 926 280 2251 981 901 262 1553 Fragments arranged by size: 2251 1553 981 926 901 280 262 197 SnaBI TAC'GTA Cuts at: 493 5142 493 Size: 4649 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7351 SphI G_CATG'C Cuts at: 1132 4709 1132 Size: 3577 3774 SspI AAT'ATT Cuts at: 5 52 5275 5 Size: 47 5223 2081 Fragments arranged by size: 5223 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7351 StyI C'CwwG_G Cuts at: 513 1065 3609 4790 513 Size: 552 2544 1181 3074 Fragments arranged by size: 3074 2544 1181 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5378 5395 5548 5733 6178 325 Size: 1255 3798 17 153 185 445 1498 Fragments arranged by size: 3798 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4489 5597 7309 Size: 274 80 33 51 1443 2512 1108 1712 Cuts at: 7309 96 Size: 138 Fragments arranged by size: 2512 1712 1443 1108 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4112 4144 7311 7343 4112 Size: 32 3167 32 4120 Fragments arranged by size: 4120 3167 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7351 XbaI T'CTAG_A Cuts at: 4687 4687 Size: 7351 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2531 3528 2531 Size: 997 6354 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7351 XmaI C'CCGG_G Cuts at: 1870 3406 4676 1870 Size: 1536 1270 4545 Fragments arranged by size: 4545 1536 1270 XmnI GAAnn'nnTTC Cuts at: 5480 5480 Size: 7351 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5159 276 Size: 53 83 186 936 3625 2468 Fragments arranged by size: 3625 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI