(Linear) (Six-base) MAPSORT of: KIEE9628.seq Check: 550 from: 1 to: 2646 KIEE9628.orf With 182 enzymes: SgfI * July 3, 2012 19:03 .. AccI GT'mk_AC Cuts at: 0 127 2227 2646 Size: 127 2100 419 Fragments arranged by size: 2100 419 127 Acc65I G'GTAC_C Cuts at: 0 772 2646 Size: 772 1874 AclI AA'CG_TT Cuts at: 0 437 2646 Size: 437 2209 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1049 2646 Size: 1049 1597 AflIII A'CryG_T Cuts at: 0 2513 2646 Size: 2513 133 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 950 982 2646 Size: 950 32 1664 Fragments arranged by size: 1664 950 32 ApoI r'AATT_y Cuts at: 0 505 1312 1375 2646 Size: 505 807 63 1271 Fragments arranged by size: 1271 807 505 63 AseI AT'TA_AT Cuts at: 0 952 2646 Size: 952 1694 AvaI C'yCGr_G Cuts at: 0 26 1401 2646 Size: 26 1375 1245 Fragments arranged by size: 1375 1245 26 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 356 389 1779 1812 2646 Size: 356 33 1390 33 834 Fragments arranged by size: 1390 834 356 33 33 BanI G'GyrC_C Cuts at: 0 66 772 2646 Size: 66 706 1874 Fragments arranged by size: 1874 706 66 BanII G_rGCy'C Cuts at: 0 39 113 1911 2476 2646 Size: 39 74 1798 565 170 Fragments arranged by size: 1798 565 170 74 39 BbsI GAAGACnn'nnnn_ Cuts at: 0 1037 2646 Size: 1037 1609 BbvCI CC'TCA_GC Cuts at: 0 1196 2646 Size: 1196 1450 BciVI GTATCCnnnnn_n' Cuts at: 0 1753 2646 Size: 1753 893 BclI T'GATC_A Cuts at: 0 489 2646 Size: 489 2157 BglII A'GATC_T Cuts at: 0 2093 2646 Size: 2093 553 BmgBI CAC'GTC Cuts at: 0 650 2646 Size: 650 1996 BmrI ACTGGGnnnn_n' Cuts at: 0 151 2646 Size: 151 2495 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1927 2646 Size: 1927 719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2110 2327 2646 Size: 2110 217 319 Fragments arranged by size: 2110 319 217 Bpu10I CC'TnA_GC Cuts at: 0 1196 2646 Size: 1196 1450 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 203 2490 2646 Size: 203 2287 156 Fragments arranged by size: 2287 203 156 BsaBI GATnn'nnATC Cuts at: 0 467 1741 2646 Size: 467 1274 905 Fragments arranged by size: 1274 905 467 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2232 2262 2646 Size: 2232 30 384 Fragments arranged by size: 2232 384 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 206 209 218 221 1919 2646 Size: 206 3 9 3 1698 727 Fragments arranged by size: 1698 727 206 9 3 3 BseYI C'CCAG_C Cuts at: 0 247 1880 2646 Size: 247 1633 766 Fragments arranged by size: 1633 766 247 BsiEI CG_ry'CG Cuts at: 0 139 2646 Size: 139 2507 BsiHKAI G_wGCw'C Cuts at: 0 87 213 589 2646 Size: 87 126 376 2057 Fragments arranged by size: 2057 376 126 87 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2638 2646 Size: 2638 8 Bsp1286I G_dGCh'C Cuts at: 0 39 87 113 213 589 1911 2476 2646 Size: 39 48 26 100 376 1322 565 170 Fragments arranged by size: 1322 565 376 170 100 48 39 26 BsrBI CCG'CTC Cuts at: 0 567 2646 Size: 567 2079 BsrDI GCAATG_nn' Cuts at: 0 1517 2197 2646 Size: 1517 680 449 Fragments arranged by size: 1517 680 449 BssSI C'ACGA_G Cuts at: 0 2584 2646 Size: 2584 62 BstYI r'GATC_y Cuts at: 0 2093 2646 Size: 2093 553 Bsu36I CC'TnA_GG Cuts at: 0 242 2646 Size: 242 2404 BtgI C'CryG_G Cuts at: 0 1604 2646 Size: 1604 1042 BtsI GCAGTG_nn' Cuts at: 0 1696 2646 Size: 1696 950 ClaI AT'CG_AT Cuts at: 0 2140 2646 Size: 2140 506 DraIII CAC_nnn'GTG Cuts at: 0 1174 2646 Size: 1174 1472 EaeI y'GGCC_r Cuts at: 0 136 553 2646 Size: 136 417 2093 Fragments arranged by size: 2093 417 136 EagI C'GGCC_G Cuts at: 0 136 2646 Size: 136 2510 EarI CTCTTCn'nnn_ Cuts at: 0 1401 2066 2646 Size: 1401 665 580 Fragments arranged by size: 1401 665 580 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1049 2110 2327 2646 Size: 1049 1061 217 319 Fragments arranged by size: 1061 1049 319 217 EcoNI CCTnn'n_nnAGG Cuts at: 0 1915 2646 Size: 1915 731 EcoO109I rG'GnC_Cy Cuts at: 0 76 1155 2646 Size: 76 1079 1491 Fragments arranged by size: 1491 1079 76 EcoRI G'AATT_C Cuts at: 0 1312 2646 Size: 1312 1334 EcoRV GAT'ATC Cuts at: 0 404 1974 2646 Size: 404 1570 672 Fragments arranged by size: 1570 672 404 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2218 2646 Size: 2218 428 HaeII r_GCGC'y Cuts at: 0 265 519 2554 2646 Size: 265 254 2035 92 Fragments arranged by size: 2035 265 254 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 393 425 520 552 1642 1674 2646 Size: 393 32 95 32 1090 32 972 Fragments arranged by size: 1090 972 393 95 32 32 32 HincII GTy'rAC Cuts at: 0 128 441 2228 2369 2627 2646 Size: 128 313 1787 141 258 19 Fragments arranged by size: 1787 313 258 141 128 19 KpnI G_GTAC'C Cuts at: 0 776 2646 Size: 776 1870 MfeI C'AATT_G Cuts at: 0 962 2646 Size: 962 1684 MslI CAynn'nnrTG Cuts at: 0 1615 2278 2646 Size: 1615 663 368 Fragments arranged by size: 1615 663 368 MspA1I CmG'CkG Cuts at: 0 141 1200 2646 Size: 141 1059 1446 Fragments arranged by size: 1446 1059 141 NcoI C'CATG_G Cuts at: 0 1604 2646 Size: 1604 1042 NspI r_CATG'y Cuts at: 0 337 2517 2646 Size: 337 2180 129 Fragments arranged by size: 2180 337 129 PciI A'CATG_T Cuts at: 0 2513 2646 Size: 2513 133 PflMI CCAn_nnn'nTGG Cuts at: 0 1824 2574 2646 Size: 1824 750 72 Fragments arranged by size: 1824 750 72 PfoI T'CCnGG_A Cuts at: 0 2343 2646 Size: 2343 303 PpuMI rG'GwC_Cy Cuts at: 0 76 2646 Size: 76 2570 PshAI GACnn'nnGTC Cuts at: 0 1864 2646 Size: 1864 782 PsiI TTA'TAA Cuts at: 0 2444 2522 2646 Size: 2444 78 124 Fragments arranged by size: 2444 124 78 PstI C_TGCA'G Cuts at: 0 146 176 1703 2646 Size: 146 30 1527 943 Fragments arranged by size: 1527 943 146 30 PvuII CAG'CTG Cuts at: 0 1200 2646 Size: 1200 1446 SalI G'TCGA_C Cuts at: 0 126 2226 2646 Size: 126 2100 420 Fragments arranged by size: 2100 420 126 SanDI GG'GwC_CC Cuts at: 0 76 2646 Size: 76 2570 SapI GCTCTTCn'nnn_ Cuts at: 0 2066 2646 Size: 2066 580 SexAI A'CCwGG_T Cuts at: 0 302 2646 Size: 302 2344 SfcI C'TryA_G Cuts at: 0 142 172 993 1080 1539 1585 1699 2646 Size: 142 30 821 87 459 46 114 947 Fragments arranged by size: 947 821 459 142 114 87 46 30 SmaI CCC'GGG Cuts at: 0 1403 2646 Size: 1403 1243 SmlI C'TyrA_G Cuts at: 0 218 2469 2646 Size: 218 2251 177 Fragments arranged by size: 2251 218 177 StyI C'CwwG_G Cuts at: 0 1604 2646 Size: 1604 1042 TatI w'GTAC_w Cuts at: 0 2484 2646 Size: 2484 162 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2107 2139 2646 Size: 2107 32 507 Fragments arranged by size: 2107 507 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 526 1523 2646 Size: 526 997 1123 Fragments arranged by size: 1123 997 526 XmaI C'CCGG_G Cuts at: 0 1401 2646 Size: 1401 1245 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AloI ApoI AseI AvaI BaeI BanI BanII BbsI BbvCI BciVI BclI BglII BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BssSI BstYI Bsu36I BtgI BtsI ClaI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII KpnI MfeI MslI MspA1I NcoI NspI PciI PflMI PfoI PpuMI PshAI PsiI PstI PvuII SalI SanDI SapI SexAI SfcI SmaI SmlI StyI TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI BcgI BfrBI BglI BlpI Bme1580I BmtI BsaI BsaAI BsaHI BsaWI BsgI BsiWI BsmI BspEI BspHI BspMI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI CspCI DraI DrdI EciI EcoICRI FseI FspI FspAI HindIII HpaI KasI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI