################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BanI BbeI BbrPI BbsI BcgI BclI BlnI BpuAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 625 GTCTAC GT^[AC][GT]AC AccII 944 CGCG CG^CG AflII 69 CTTAAG C^TTAAG AlwNI 856 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1174 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 135 GAGCTC G[AT]GC[AT]^C AvaI 736 CCCGAG C^[CT]CG[GA]G AviII 641 TGCGCA TGC^GCA BciVI 900 GGATAC GTATCC(6/5) BfrI 69 CTTAAG C^TTAAG BglI 1156 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 468 AGATCT A^GATCT BlpI 729 GCTCAGC GC^T[ACGT]AGC Bpu1102I 729 GCTCAGC GC^T[ACGT]AGC BsaAI 286 TACGTG [CT]AC^GT[GA] BsaI 42 GGTCTC GGTCTC(1/5) BseRI 922 GAGGAG GAGGAG(10/8) BsiHKAI 135 GAGCTC G[AT]GC[AT]^C BsmFI 829 GTCCC GGGAC(10/14) BsoBI 736 CCCGAG C^[CT]CG[GA]G BspHI 173 TCATGA T^CATGA BstAPI 528 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 944 CGCG CG^CG BstXI 323 CCAATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 468 AGATCT [GA]^GATC[CT] CelII 729 GCTCAGC GC^T[ACGT]AGC DraIII 1060 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 666 CCATGG C^C[AT][AT]GG FauI 916 CCCGC CCCGC(4/6) FspI 641 TGCGCA TGC^GCA HindIII 314 AAGCTT A^AGCTT MflI 468 AGATCT [GA]^GATC[CT] MvnI 944 CGCG CG^CG NcoI 666 CCATGG C^CATGG NspI 357 GCATGT [GA]CATG^[CT] PstI 526 CTGCAG CTGCA^G RcaI 173 TCATGA T^CATGA SacI 135 GAGCTC GAGCT^C SapI 1264 GAAGAGC GCTCTTC(1/4) SbfI 525 CCTGCAGG CCTGCA^GG SmlI 69 CTTAAG C^T[CT][GA]AG Sse8387I 525 CCTGCAGG CCTGCA^GG StyI 666 CCATGG C^C[AT][AT]GG XhoII 468 AGATCT [GA]^GATC[CT] XmnI 1174 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BalI 815 TGGCCA TGG^CCA 953 TGGCCA BanII 135 GAGCTC G[GA]GC[CT]^C 936 GAGCCC BbvCI 651 CCTCAGC CCTCAGC(-5/-2) 919 GCTGAGG Bsp1407I 478 TGTACA T^GTACA 573 TGTACA BsrGI 478 TGTACA T^GTACA 573 TGTACA DsaI 666 CCATGG C^C[GA][CT]GG 724 CCGTGG EarI 370 CTCTTC CTCTTC(1/4) 1264 GAAGAG Ksp632I 370 CTCTTC CTCTTC(1/4) 1264 GAAGAG MaeII 287 ACGT A^CGT 1152 ACGT MluNI 815 TGGCCA TGG^CCA 953 TGGCCA MscI 815 TGGCCA TGG^CCA 953 TGGCCA MslI 577 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 852 CACCCAGATG MspA1I 673 CAGCGG C[AC]G^C[GT]G 917 CCGCTG PflMI 558 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1059 CCACTTTGTGG PleI 1012 GACTC GAGTC(4/5) 142 GAGTC PpuMI 239 AGGACCT [GA]G^G[AT]CC[CT] 740 AGGTCCT SfcI 526 CTGCAG C^T[GA][CT]AG 786 CTACAG SspBI 478 TGTACA T^GTACA 573 TGTACA TaqI 399 TCGA T^CGA 548 TCGA Tsp509I 1175 AATT ^AATT 1204 AATT TthHB8I 399 TCGA T^CGA 548 TCGA Van91I 558 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1059 CCACTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp45I TspRI