(Circular) (Six-base) MAPSORT of: pFN21AE0949.seq Check: 9472 from: 1 to: 5998 pFN21AE0949 5998 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2631 3341 1937 Size: 694 710 4594 Fragments arranged by size: 4594 710 694 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2564 3068 4061 5445 5713 1632 Size: 932 504 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 932 504 268 AflII C'TTAA_G Cuts at: 820 1017 2074 820 Size: 197 1057 4744 Fragments arranged by size: 4744 1057 197 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 2586 1166 Size: 1420 4578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5998 AlwNI CAG_nnn'CTG Cuts at: 2866 3741 5313 2866 Size: 875 1572 3551 Fragments arranged by size: 3551 1572 875 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 3307 3533 3685 5794 1493 Size: 1814 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 2741 3323 1870 Size: 73 798 582 4545 Fragments arranged by size: 4545 798 582 73 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3319 4774 618 Size: 325 217 627 1532 1455 1842 Fragments arranged by size: 1842 1532 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2144 2945 3317 1391 Size: 753 801 372 4072 Fragments arranged by size: 4072 801 753 372 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BbvCI CC'TCA_GC Cuts at: 2657 2925 2657 Size: 268 5730 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4189 4223 1903 Size: 34 2252 34 3678 Fragments arranged by size: 3678 2252 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2899 3890 5100 2899 Size: 991 1210 3797 Fragments arranged by size: 3797 1210 991 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3167 4609 136 Size: 107 122 71 631 605 1495 1442 1525 Fragments arranged by size: 1525 1495 1442 631 605 122 107 71 BglII A'GATC_T Cuts at: 2473 5993 2473 Size: 3520 2478 BlpI GC'TnA_GC Cuts at: 2735 3415 2735 Size: 680 5318 Bme1580I G_kGCm'C Cuts at: 1790 2895 4059 5215 1790 Size: 1105 1164 1156 2573 Fragments arranged by size: 2573 1164 1156 1105 BmgBI CAC'GTC Cuts at: 1318 3158 1318 Size: 1840 4158 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3102 3729 4687 Size: 416 53 83 399 24 1685 627 958 Cuts at: 4687 442 Size: 1753 Fragments arranged by size: 1753 1685 958 627 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 2747 3296 4658 1239 Size: 1496 12 549 1362 2579 Fragments arranged by size: 2579 1496 1362 549 12 Bpu10I CC'TnA_GC Cuts at: 2657 2925 2657 Size: 268 5730 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4123 4988 5286 4123 Size: 865 298 4835 Fragments arranged by size: 4835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2053 4661 882 Size: 617 554 2608 2219 Fragments arranged by size: 2608 2219 617 554 BsaAI yAC'GTr Cuts at: 493 1204 2293 3789 493 Size: 711 1089 1496 2702 Fragments arranged by size: 2702 1496 1089 711 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3805 4187 4822 275 Size: 53 83 186 936 2272 382 635 1451 Fragments arranged by size: 2272 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4431 4829 5103 5250 5661 4431 Size: 398 274 147 411 4768 Fragments arranged by size: 4768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2277 2307 3008 3038 1178 Size: 30 165 30 874 30 701 30 4138 Fragments arranged by size: 4138 874 701 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2942 1186 Size: 1756 4242 BseYI C'CCAG_C Cuts at: 5201 5697 5201 Size: 496 5502 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5425 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2144 4059 4144 5215 2144 Size: 1915 85 1071 2927 Fragments arranged by size: 2927 1915 1071 85 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2895 2945 3317 4059 4144 5215 Size: 399 354 751 50 372 742 85 1071 Cuts at: 5215 1391 Size: 2174 Fragments arranged by size: 2174 1071 751 742 399 372 354 85 50 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 2178 3887 5617 2178 Size: 1709 1730 2559 Fragments arranged by size: 2559 1730 1709 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3353 844 Size: 2509 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3885 1394 Size: 429 2062 3507 Fragments arranged by size: 3507 2062 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4493 4667 65 Size: 1183 3245 174 1396 Fragments arranged by size: 3245 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4642 4829 1700 Size: 110 2832 187 2869 Fragments arranged by size: 2869 2832 187 110 BsrGI T'GTAC_A Cuts at: 96 2483 2578 96 Size: 2387 95 3516 Fragments arranged by size: 3516 2387 95 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 4058 5070 4058 Size: 1012 4986 BstAPI GCAn_nnn'nTGC Cuts at: 794 2539 794 Size: 1745 4253 BstBI TT'CG_AA Cuts at: 4802 4802 Size: 5998 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2335 2335 Size: 5998 BstYI r'GATC_y Cuts at: 1972 2473 3328 3695 4080 4097 5549 5635 5647 Size: 501 855 367 385 17 1452 86 12 Cuts at: 5647 5993 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 855 501 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2671 2729 513 Size: 552 1606 58 3782 Fragments arranged by size: 3782 1606 552 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5259 Fragments arranged by size: 5259 683 56 BtsI GCAGTG_nn' Cuts at: 3083 3514 4308 4328 3083 Size: 431 794 20 4753 Fragments arranged by size: 4753 794 431 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2398 2433 529 Size: 35 1834 35 4094 Fragments arranged by size: 4094 1834 35 35 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DraIII CAC_nnn'GTG Cuts at: 3070 3070 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2820 2827 2958 Size: 54 1425 50 275 18 990 7 131 Cuts at: 2958 3445 4334 5963 8 Size: 487 889 1629 43 Fragments arranged by size: 1629 1425 990 889 487 275 131 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2381 3264 3928 1353 Size: 1028 883 664 3423 Fragments arranged by size: 3423 1028 883 664 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4585 4959 5105 387 Size: 1064 138 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2142 2142 Size: 5998 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2564 2735 2747 3068 3296 4061 4658 Size: 393 932 171 12 321 228 765 597 Cuts at: 4658 5445 5713 1239 Size: 787 268 1524 Fragments arranged by size: 1524 932 787 765 597 393 321 268 228 171 12 EcoO109I rG'GnC_Cy Cuts at: 2245 2548 2746 2245 Size: 303 198 5497 Fragments arranged by size: 5497 303 198 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FspI TGC'GCA Cuts at: 2648 4504 2648 Size: 1856 4142 HaeII r_GCGC'y Cuts at: 1678 5145 1678 Size: 3467 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2126 2158 2245 2277 2765 Size: 32 135 32 554 32 87 32 488 Cuts at: 2765 2797 3008 3040 4639 4671 4713 4745 1373 Size: 32 211 32 1599 32 42 32 2626 Fragments arranged by size: 2626 1599 554 488 211 135 87 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 2319 748 Size: 1571 4427 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2883 3033 5112 5296 1305 Size: 1578 150 2079 184 2007 Fragments arranged by size: 2079 2007 1578 184 150 MscI TGG'CCA Cuts at: 10 64 1489 1832 2822 2960 10 Size: 54 1425 343 990 138 3048 Fragments arranged by size: 3048 1425 990 343 138 54 MslI CAynn'nnrTG Cuts at: 518 1166 2586 2861 3956 4315 4474 518 Size: 648 1420 275 1095 359 159 2042 Fragments arranged by size: 2042 1420 1095 648 359 275 159 MspA1I CmG'CkG Cuts at: 1606 2680 2924 3414 4091 5239 5484 1606 Size: 1074 244 490 677 1148 245 2120 Fragments arranged by size: 2120 1148 1074 677 490 245 244 NcoI C'CATG_G Cuts at: 513 1065 2671 513 Size: 552 1606 3840 Fragments arranged by size: 3840 1606 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 2366 3356 4901 1132 Size: 97 1137 990 1545 2229 Fragments arranged by size: 2229 1545 1137 990 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5998 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 2569 3070 1108 Size: 1461 501 4036 Fragments arranged by size: 4036 1461 501 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2746 2245 Size: 501 5497 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PstI C_TGCA'G Cuts at: 830 2535 3350 830 Size: 1705 815 3478 Fragments arranged by size: 3478 1705 815 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 SacI G_AGCT'C Cuts at: 2144 2144 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SapI GCTCTTCn'nnn_ Cuts at: 3264 3264 Size: 5998 SbfI CC_TGCA'GG Cuts at: 2535 3350 2535 Size: 815 5183 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 2531 2791 3346 4481 5162 5353 826 Size: 219 1486 260 555 1135 681 191 1471 Fragments arranged by size: 1486 1471 1135 681 555 260 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2074 4102 5003 5265 820 Size: 197 926 131 2028 901 262 1553 Fragments arranged by size: 2028 1553 926 901 262 197 131 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2671 3437 513 Size: 552 1606 766 3074 Fragments arranged by size: 3074 1606 766 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2873 4025 4042 4195 4380 4825 325 Size: 1255 1293 1152 17 153 185 445 1498 Fragments arranged by size: 1498 1293 1255 1152 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2483 2578 2709 Size: 274 80 33 51 1443 506 95 131 Cuts at: 2709 4244 5956 96 Size: 1535 1712 138 Fragments arranged by size: 1712 1535 1443 506 274 138 131 95 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2277 2309 5958 5990 2277 Size: 32 3649 32 2285 Fragments arranged by size: 3649 2285 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 3183 4127 3183 Size: 944 5054 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI