################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MroI MseI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 253 GAATTT [GA]^AATT[CT] AflIII 80 ACGTGT A^C[GA][CT]GT AocI 324 CCTTAGG CC^T[ACGT]AGG ApoI 253 GAATTT [GA]^AATT[CT] BalI 2 TGGCCA TGG^CCA BbrPI 79 CACGTG CAC^GTG BcnI 584 CCCGG CC^[GC]GG BsaAI 79 CACGTG [CT]AC^GT[GA] BseRI 650 CTCCTC GAGGAG(10/8) BspHI 197 TCATGA T^CATGA BsrFI 523 GCCGGC [GA]^CCGG[CT] BstF5I 427 GGATG GGATG(2/0) Bsu36I 324 CCTTAGG CC^T[ACGT]AGG Cfr10I 523 GCCGGC [GA]^CCGG[CT] EaeI 2 TGGCCA [CT]^GGCC[GA] EarI 273 GAAGAG CTCTTC(1/4) Eco81I 324 CCTTAGG CC^T[ACGT]AGG EcoRV 181 GATATC GAT^ATC FauI 74 GCGGG CCCGC(4/6) FokI 427 GGATG GGATG(9/13) Ksp632I 273 GAAGAG CTCTTC(1/4) MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MslI 422 CACACGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 523 GCCGGC GCC^GGC NciI 584 CCCGG CC^[GC]GG NgoMIV 523 GCCGGC G^CCGGC NlaIV 573 GGCCCC GG[ACGT]^[ACGT]CC PleI 469 GAGTC GAGTC(4/5) PmaCI 79 CACGTG CAC^GTG PmlI 79 CACGTG CAC^GTG PstI 340 CTGCAG CTGCA^G RcaI 197 TCATGA T^CATGA ScaI 224 AGTACT AGT^ACT SfaNI 428 GATGC GCATC(5/9) SmlI 557 CTCAAG C^T[CT][GA]AG TaqI 472 TCGA T^CGA Tsp509I 254 AATT ^AATT TthHB8I 472 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 48 GACGTC GACGT^C 629 GACGTC AciI 37 CCGC CCGC(-3/-1) 74 GCGG AluI 138 AGCT AG^CT 384 AGCT BfaI 308 CTAG C^TAG 656 CTAG BmyI 76 GGGCAC G[AGT]GC[ACT]^C 581 GTGCCC BpmI 228 CTCCAG CTGGAG(16/14) 466 CTGGAG BsaJI 99 CCTCGG C^C[ACGT][ACGT]GG 230 CCAGGG BsmI 121 GAATGC GAATGC(1/-1) 190 GCATTC Bsp1286I 76 GGGCAC G[AGT]GC[ACT]^C 581 GTGCCC BstNI 186 CCAGG CC^[AT]GG 230 CCAGG Cfr13I 526 GGCCC G^G[ACGT]CC 573 GGCCC DdeI 325 CTTAG C^T[ACGT]AG 512 CTGAG DpnII 368 GATC ^GATC 484 GATC EcoRII 186 CCAGG ^CC[AT]GG 230 CCAGG HapII 524 CCGG C^CGG 585 CCGG HgaI 91 GACGC GACGC(5/10) 175 GACGC HpaII 524 CCGG C^CGG 585 CCGG MaeI 308 CTAG C^TAG 656 CTAG MboI 368 GATC ^GATC 484 GATC MspI 524 CCGG C^CGG 585 CCGG MvaI 186 CCAGG CC^[AT]GG 230 CCAGG NdeII 368 GATC ^GATC 484 GATC PshAI 464 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 629 GACGTCTGTC Sau3AI 368 GATC ^GATC 484 GATC Sau96I 526 GGCCC G^G[ACGT]CC 573 GGCCC SfcI 291 CTATAG C^T[GA][CT]AG 340 CTGCAG TfiI 319 GAATC G^A[AT]TC 536 GAATC TspRI 335 CAGTG CA[GC]TG[ACGT][ACGT]^ 641 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AcyI AfaI BbvI BsaHI BsiYI BslI BsrI BssKI Cac8I Fnu4HI HaeIII Hin1I HinfI HphI ItaI MaeII MboII MnlI MwoI NlaIII RsaI ScrFI TseI