################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BciVI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1602 GGTACC G^GTACC Asp700I 483 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1602 GGTACC G^GTACC AvaII 912 GGACC G^G[AT]CC BalI 1014 TGGCCA TGG^CCA BanII 1334 GAGCTC G[GA]GC[CT]^C BbvCI 659 CCTCAGC CCTCAGC(-5/-2) BcgI 1599 CGAGGTACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1163 CCCGG CC^[GC]GG BfaI 526 CTAG C^TAG BglI 67 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1656 GCTCAGC GC^T[ACGT]AGC BpmI 784 CTCCAG CTGGAG(16/14) Bpu1102I 1656 GCTCAGC GC^T[ACGT]AGC BsiEI 1045 CGACCG CG[GA][CT]^CG BsmBI 1619 CGTCTC CGTCTC(1/5) BsmI 1431 GCATTC GAATGC(1/-1) BspHI 837 TCATGA T^CATGA BspMI 1605 ACCTGC ACCTGC(4/8) BsrFI 25 GCCGGC [GA]^CCGG[CT] BstEII 633 GGTTACC G^GT[ACGT]ACC BstNI 777 CCTGG CC^[AT]GG BstPI 633 GGTTACC G^GT[ACGT]ACC CelII 1656 GCTCAGC GC^T[ACGT]AGC Cfr10I 25 GCCGGC [GA]^CCGG[CT] EcoO65I 633 GGTTACC G^GT[ACGT]ACC EcoRII 777 CCTGG ^CC[AT]GG EcoRV 1530 GATATC GAT^ATC KpnI 1602 GGTACC GGTAC^C MaeI 526 CTAG C^TAG MluNI 1014 TGGCCA TGG^CCA MscI 1014 TGGCCA TGG^CCA MslI 1092 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 777 CCTGG CC^[AT]GG NaeI 25 GCCGGC GCC^GGC NciI 1163 CCCGG CC^[GC]GG NdeI 800 CATATG CA^TATG NgoMIV 25 GCCGGC G^CCGGC NspI 1148 GCATGC [GA]CATG^[CT] PshAI 895 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 88 CTGCAG CTGCA^G PvuII 144 CAGCTG CAG^CTG RcaI 837 TCATGA T^CATGA SacI 1334 GAGCTC GAGCT^C SapI 1426 GAAGAGC GCTCTTC(1/4) ScaI 749 AGTACT AGT^ACT SfcI 88 CTGCAG C^T[GA][CT]AG SmlI 916 CTTGAG C^T[CT][GA]AG SphI 1148 GCATGC GCATG^C StuI 207 AGGCCT AGG^CCT Tsp45I 170 GTGAC ^GT[GC]AC XcmI 1091 CCATGGCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 483 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 215 GTCTAC GT^[AC][GT]AC 1116 GTCTAC BbsI 1322 GAAGAC GAAGAC(2/6) 284 GTCTTC BclI 305 TGATCA T^GATCA 1710 TGATCA BpuAI 1322 GAAGAC GAAGAC(2/6) 284 GTCTTC BseRI 1550 CTCCTC GAGGAG(10/8) 1357 GAGGAG BsmAI 1284 GAGAC GTCTC(1/5) 1620 GTCTC BssKI 777 CCTGG ^CC[ACGT]GG 1163 CCCGG BstXI 1005 CCACTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1091 CCATGGCTGTGG DraII 773 AGGGCCT [GA]G^G[ACGT]CC[CT] 1648 GGGGCCT DsaI 1091 CCATGG C^C[GA][CT]GG 1347 CCATGG EaeI 1014 TGGCCA [CT]^GGCC[GA] 1103 TGGCCG EcoO109I 773 AGGGCCT [GA]G^G[ACGT]CC[CT] 1648 GGGGCCT EcoRI 259 GAATTC G^AATTC 921 GAATTC FbaI 305 TGATCA T^GATCA 1710 TGATCA HapII 26 CCGG C^CGG 1164 CCGG HpaII 26 CCGG C^CGG 1164 CCGG HphI 1311 TCACC GGTGA(8/7) 1483 TCACC MaeII 302 ACGT A^CGT 1492 ACGT MspA1I 55 CCGCTG C[AC]G^C[GT]G 144 CAGCTG MspI 26 CCGG C^CGG 1164 CCGG MwoI 67 GCCATCGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1096 GCTGTGGTGGC NcoI 1091 CCATGG C^CATGG 1347 CCATGG ScrFI 777 CCTGG CC^[ACGT]GG 1163 CCCGG SspI 485 AATATT AAT^ATT 813 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI BanI BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BstF5I BstYI Cac8I Cfr13I DdeI DpnII EarI EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII