(Circular) (Six-base) MAPSORT of: pFN21AE0980.seq Check: 6253 from: 1 to: 6439 pFN21AE0980 6439 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4249 278 Size: 53 83 186 936 2713 2468 Fragments arranged by size: 2713 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2221 3122 3782 1937 Size: 284 901 660 4594 Fragments arranged by size: 4594 901 660 284 Acc65I G'GTAC_C Cuts at: 3607 3760 3607 Size: 153 6286 AclI AA'CG_TT Cuts at: 1563 4566 4939 1563 Size: 3003 373 3063 Fragments arranged by size: 3063 3003 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2852 4502 5886 6154 1632 Size: 644 576 1650 1384 268 1917 Fragments arranged by size: 1917 1650 1384 644 576 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6242 AflIII A'CryG_T Cuts at: 1203 5338 6315 1203 Size: 4135 977 1327 Fragments arranged by size: 4135 1327 977 AgeI A'CCGG_T Cuts at: 5270 5270 Size: 6439 AhdI GACnn_n'nnGTC Cuts at: 5168 5168 Size: 6439 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2057 1184 Size: 873 5566 AlwNI CAG_nnn'CTG Cuts at: 2092 2782 3476 4182 5754 2092 Size: 690 694 706 1572 2777 Fragments arranged by size: 2777 1572 706 694 690 ApaLI G'TGCA_C Cuts at: 4496 5652 4496 Size: 1156 5283 ApoI r'AATT_y Cuts at: 1493 2133 2264 2926 3676 3748 3974 4126 6235 Size: 640 131 662 750 72 226 152 2109 Cuts at: 6235 1493 Size: 1697 Fragments arranged by size: 2109 1697 750 662 640 226 152 131 72 AseI AT'TA_AT Cuts at: 160 1032 4993 160 Size: 872 3961 1606 Fragments arranged by size: 3961 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 AvaI C'yCGr_G Cuts at: 1870 1943 3764 1870 Size: 73 1821 4545 Fragments arranged by size: 4545 1821 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2819 2852 3591 3624 2819 Size: 33 739 33 5634 Fragments arranged by size: 5634 739 33 33 BamHI G'GATC_C Cuts at: 3769 4136 3769 Size: 367 6072 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 3064 3607 3760 5215 Size: 325 217 627 269 1008 543 153 1455 Cuts at: 5215 618 Size: 1842 Fragments arranged by size: 1842 1455 1008 627 543 325 269 217 153 BanII G_rGCy'C Cuts at: 1391 3343 3758 1391 Size: 1952 415 4072 Fragments arranged by size: 4072 1952 415 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 3334 928 Size: 971 383 1052 4033 Fragments arranged by size: 4033 1052 971 383 BbvCI CC'TCA_GC Cuts at: 2665 2665 Size: 6439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3593 3627 4630 4664 1903 Size: 34 1656 34 1003 34 3678 Fragments arranged by size: 3678 1656 1003 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4331 5541 4331 Size: 1210 5229 BclI T'GATC_A Cuts at: 1548 2310 3715 1548 Size: 762 1405 4272 Fragments arranged by size: 4272 1405 762 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 5050 136 Size: 107 122 71 631 605 406 2972 1525 Fragments arranged by size: 2972 1525 631 605 406 122 107 71 BglII A'GATC_T Cuts at: 6434 6434 Size: 6439 BlpI GC'TnA_GC Cuts at: 3662 3856 3662 Size: 194 6245 Bme1580I G_kGCm'C Cuts at: 1790 4500 5656 1790 Size: 2710 1156 2573 Fragments arranged by size: 2710 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3498 1318 Size: 2180 4259 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4170 5128 442 Size: 416 53 83 399 24 2753 958 1753 Fragments arranged by size: 2753 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2774 5099 1239 Size: 1535 2325 2579 Fragments arranged by size: 2579 2325 1535 Bpu10I CC'TnA_GC Cuts at: 2665 2665 Size: 6439 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2942 4564 5429 5727 2942 Size: 1622 865 298 3654 Fragments arranged by size: 3654 1622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5102 882 Size: 617 3603 2219 Fragments arranged by size: 3603 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4230 493 Size: 711 3026 2702 Fragments arranged by size: 3026 2702 711 BsaBI GATnn'nnATC Cuts at: 6433 6433 Size: 6439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4246 4628 5263 275 Size: 53 83 186 936 2713 382 635 1451 Fragments arranged by size: 2713 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4872 5270 5544 5691 6102 4872 Size: 398 274 147 411 5209 Fragments arranged by size: 5209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3440 3470 1178 Size: 30 165 30 2037 30 4147 Fragments arranged by size: 4147 2037 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3377 3546 1186 Size: 2191 169 4079 Fragments arranged by size: 4079 2191 169 BseYI C'CCAG_C Cuts at: 2541 3615 5642 6138 2541 Size: 1074 2027 496 2842 Fragments arranged by size: 2842 2027 1074 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5866 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3053 3889 4650 5678 1540 Size: 275 186 1052 836 761 1028 2301 Fragments arranged by size: 2301 1052 1028 836 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3343 3602 3727 4500 4585 5656 3343 Size: 259 125 773 85 1071 4126 Fragments arranged by size: 4126 1071 773 259 125 85 BsmI GAATG_Cn' Cuts at: 3436 3950 4043 3436 Size: 514 93 5832 Fragments arranged by size: 5832 514 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3630 3630 Size: 6439 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3343 3602 3727 3758 4500 4585 5656 Size: 399 1553 259 125 31 742 85 1071 Cuts at: 5656 1391 Size: 2174 Fragments arranged by size: 2174 1553 1071 742 399 259 125 85 31 BspEI T'CCGG_A Cuts at: 6102 6102 Size: 6439 BspHI T'CATG_A Cuts at: 2842 4328 6058 2842 Size: 1486 1730 3223 Fragments arranged by size: 3223 1730 1486 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3619 3794 844 Size: 2775 175 3489 Fragments arranged by size: 3489 2775 175 BsrBI CCG'CTC Cuts at: 1394 1823 4326 1394 Size: 429 2503 3507 Fragments arranged by size: 3507 2503 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4934 5108 65 Size: 1183 3686 174 1396 Fragments arranged by size: 3686 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 5083 5270 1700 Size: 110 220 3053 187 2869 Fragments arranged by size: 3053 2869 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6439 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6439 BssSI C'ACGA_G Cuts at: 4499 5511 4499 Size: 1012 5427 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6439 BstBI TT'CG_AA Cuts at: 5243 5243 Size: 6439 BstEII G'GTnAC_C Cuts at: 1184 2638 1184 Size: 1454 4985 BstXI CCAn_nnnn'nTGG Cuts at: 3017 3103 3017 Size: 86 6353 BstYI r'GATC_y Cuts at: 1972 2362 2710 3234 3356 3473 3769 4136 4521 Size: 390 348 524 122 117 296 367 385 Cuts at: 4521 4538 5990 6076 6088 6434 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 524 390 385 367 348 346 296 122 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3096 3352 513 Size: 552 939 1092 256 3600 Fragments arranged by size: 3600 1092 939 552 256 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3210 3492 487 Size: 683 56 1984 282 3434 Fragments arranged by size: 3434 1984 683 282 56 BtsI GCAGTG_nn' Cuts at: 3010 3955 4749 4769 3010 Size: 945 794 20 4680 Fragments arranged by size: 4680 945 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6439 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6404 DraI TTT'AAA Cuts at: 3743 3743 Size: 6439 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5446 809 Size: 507 4130 1802 Fragments arranged by size: 4130 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3019 3108 3886 Size: 54 1425 50 275 18 1189 89 778 Cuts at: 3886 4775 6404 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1189 889 778 275 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3886 1537 Size: 275 2074 4090 Fragments arranged by size: 4090 2074 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 3281 3426 4369 1353 Size: 879 1049 145 943 3423 Fragments arranged by size: 3423 1049 943 879 145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5026 5400 5546 387 Size: 1064 138 3437 374 146 1280 Fragments arranged by size: 3437 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3341 3341 Size: 6439 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2774 2852 4502 5099 5886 6154 Size: 393 644 498 78 1650 597 787 268 Cuts at: 6154 1239 Size: 1524 Fragments arranged by size: 1650 1524 787 644 597 498 393 268 78 EcoO109I rG'GnC_Cy Cuts at: 2779 3654 2779 Size: 875 5564 EcoRI G'AATT_C Cuts at: 2264 2926 3748 4126 2264 Size: 662 822 378 4577 Fragments arranged by size: 4577 822 662 378 EcoRV GAT'ATC Cuts at: 3537 3537 Size: 6439 FspI TGC'GCA Cuts at: 4945 4945 Size: 6439 HaeII r_GCGC'y Cuts at: 1678 5586 1678 Size: 3908 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2497 2529 3325 3357 3376 Size: 32 135 32 925 32 796 32 19 Cuts at: 3376 3408 5080 5112 5154 5186 1373 Size: 32 1672 32 42 32 2626 Fragments arranged by size: 2626 1672 925 796 135 42 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 3783 4029 669 Size: 988 281 1845 246 3079 Fragments arranged by size: 3079 1845 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6439 HpaI GTT'AAC Cuts at: 4029 4029 Size: 6439 KpnI G_GTAC'C Cuts at: 3611 3764 3611 Size: 153 6286 MfeI C'AATT_G Cuts at: 4038 4038 Size: 6439 MluI A'CGCG_T Cuts at: 6315 6315 Size: 6439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2453 3096 5553 5737 1305 Size: 1148 643 2457 184 2007 Fragments arranged by size: 2457 2007 1148 643 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3021 10 Size: 54 1425 343 1189 3428 Fragments arranged by size: 3428 1425 1189 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3101 4397 4756 4915 518 Size: 648 1935 1296 359 159 2042 Fragments arranged by size: 2042 1935 1296 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2151 3855 4532 5680 5925 1606 Size: 456 89 1704 677 1148 245 2120 Fragments arranged by size: 2120 1704 1148 677 456 245 89 NaeI GCC'GGC Cuts at: 2032 2032 Size: 6439 NcoI C'CATG_G Cuts at: 513 1065 2004 3096 3352 513 Size: 552 939 1092 256 3600 Fragments arranged by size: 3600 1092 939 552 256 NdeI CA'TA_TG Cuts at: 387 2806 387 Size: 2419 4020 NgoMIV G'CCGG_C Cuts at: 2030 2030 Size: 6439 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6439 NotI GC'GGCC_GC Cuts at: 3886 3886 Size: 6439 NspI r_CATG'y Cuts at: 1132 1229 3157 3797 5342 1132 Size: 97 1928 640 1545 2229 Fragments arranged by size: 2229 1928 1545 640 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6439 PciI A'CATG_T Cuts at: 5338 5338 Size: 6439 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6439 PmeI GTTT'AAAC Cuts at: 3743 3743 Size: 6439 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4508 4540 6021 6053 4508 Size: 32 1481 32 4894 Fragments arranged by size: 4894 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2904 1613 Size: 1291 5148 PsiI TTA'TAA Cuts at: 4009 4009 Size: 6439 PstI C_TGCA'G Cuts at: 830 2097 3791 830 Size: 1267 1694 3478 Fragments arranged by size: 3478 1694 1267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6439 PvuII CAG'CTG Cuts at: 2151 2151 Size: 6439 SacI G_AGCT'C Cuts at: 3343 3343 Size: 6439 SalI G'TCGA_C Cuts at: 1936 3781 1936 Size: 1845 4594 SapI GCTCTTCn'nnn_ Cuts at: 3426 3426 Size: 6439 SbfI CC_TGCA'GG Cuts at: 3791 3791 Size: 6439 ScaI AGT'ACT Cuts at: 2756 4687 6399 2756 Size: 1931 1712 2796 Fragments arranged by size: 2796 1931 1712 SfcI C'TryA_G Cuts at: 826 1045 2093 3787 4922 5603 5794 826 Size: 219 1048 1694 1135 681 191 1471 Fragments arranged by size: 1694 1471 1135 1048 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6439 SmaI CCC'GGG Cuts at: 1872 3766 1872 Size: 1894 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2921 4543 5444 5706 820 Size: 197 926 978 1622 901 262 1553 Fragments arranged by size: 1622 1553 978 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4230 493 Size: 3737 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6439 SphI G_CATG'C Cuts at: 1132 3157 3797 1132 Size: 2025 640 3774 Fragments arranged by size: 3774 2025 640 SspI AAT'ATT Cuts at: 5 52 2492 2820 4363 5 Size: 47 2440 328 1543 2081 Fragments arranged by size: 2440 2081 1543 328 47 StuI AGG'CCT Cuts at: 1517 2214 1517 Size: 697 5742 StyI C'CwwG_G Cuts at: 513 1065 2004 2551 3096 3352 3878 513 Size: 552 939 547 545 256 526 3074 Fragments arranged by size: 3074 939 552 547 545 526 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4466 4483 4636 4821 5266 325 Size: 1255 2886 17 153 185 445 1498 Fragments arranged by size: 2886 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 4685 6397 Size: 274 80 33 51 1443 777 1931 1712 Cuts at: 6397 96 Size: 138 Fragments arranged by size: 1931 1712 1443 777 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2124 2156 6399 6431 2124 Size: 32 4243 32 2132 Fragments arranged by size: 4243 2132 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6439 XbaI T'CTAG_A Cuts at: 3775 3775 Size: 6439 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3103 2011 Size: 1092 5347 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6439 XmaI C'CCGG_G Cuts at: 1870 3764 1870 Size: 1894 4545 XmnI GAAnn'nnTTC Cuts at: 2492 4568 2492 Size: 2076 4363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4247 276 Size: 53 83 186 936 2713 2468 Fragments arranged by size: 2713 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI