################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MroI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 564 GTCTAC GT^[AC][GT]AC AccII 427 CGCG CG^CG AflIII 761 ACATGT A^C[GA][CT]GT AhdI 410 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 310 CCTGAGG CC^T[ACGT]AGG ApaI 218 GGGCCC GGGCC^C AspEI 410 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 135 TGGCCA TGG^CCA BbsI 667 GAAGAC GAAGAC(2/6) BbvCI 237 GCTGAGG CCTCAGC(-5/-2) BpuAI 667 GAAGAC GAAGAC(2/6) BsmBI 957 GAGACG CGTCTC(1/5) BspLU11I 761 ACATGT A^CATGT BsrDI 1475 GCAATG GCAATG(2/0) BstEII 366 GGTGACC G^GT[ACGT]ACC BstPI 366 GGTGACC G^GT[ACGT]ACC BstUI 427 CGCG CG^CG BstXI 837 CCAGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 310 CCTGAGG CC^T[ACGT]AGG CfoI 156 GCGC GCG^C EaeI 135 TGGCCA [CT]^GGCC[GA] Eam1105I 410 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 55 GAAGAG CTCTTC(1/4) Eco81I 310 CCTGAGG CC^T[ACGT]AGG EcoO65I 366 GGTGACC G^GT[ACGT]ACC EcoRI 1613 GAATTC G^AATTC EcoRV 1108 GATATC GAT^ATC EcoT14I 257 CCAAGG C^C[AT][AT]GG HhaI 156 GCGC GCG^C HinP1I 156 GCGC G^CGC Ksp632I 55 GAAGAG CTCTTC(1/4) MluNI 135 TGGCCA TGG^CCA MscI 135 TGGCCA TGG^CCA MseI 332 TTAA T^TAA MvnI 427 CGCG CG^CG NspI 761 ACATGT [GA]CATG^[CT] PpuMI 189 AGGACCC [GA]G^G[AT]CC[CT] PvuII 854 CAGCTG CAG^CTG SfaNI 472 GCATC GCATC(5/9) SmlI 889 CTTGAG C^T[CT][GA]AG StyI 257 CCAAGG C^C[AT][AT]GG Tru9I 332 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcsI 750 AAATTT [GA]^AATT[CT] 1613 GAATTC ApoI 750 AAATTT [GA]^AATT[CT] 1613 GAATTC AvaI 194 CCCGAG C^[CT]CG[GA]G 905 CCCGAG AvaII 99 GGACC G^G[AT]CC 190 GGACC BsmFI 98 GGGAC GGGAC(10/14) 681 GTCCC BsoBI 194 CCCGAG C^[CT]CG[GA]G 905 CCCGAG EcoNI 306 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 372 CCTATTAGAGG FauI 936 GCGGG CCCGC(4/6) 1255 GCGGG MspA1I 854 CAGCTG C[AC]G^C[GT]G 934 CAGCGG PflMI 321 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 648 CCAGACTTTGG TaqI 690 TCGA T^CGA 1686 TCGA Tsp45I 367 GTGAC ^GT[GC]AC 546 GTCAC TthHB8I 690 TCGA T^CGA 1686 TCGA Van91I 321 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 648 CCAGACTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AspHI BanII BbvI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI StuI TfiI TseI Tsp509I TspRI XhoII XmnI