(Linear) (Six-base) MAPSORT of: KIBB7881.seq Check: 1111 from: 1 to: 1734 KIBB7881.orf With 182 enzymes: SgfI * July 3, 2012 16:30 .. AccI GT'mk_AC Cuts at: 0 565 1734 Size: 565 1169 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 8 26 74 144 1059 1143 1395 1479 Size: 8 18 48 70 915 84 252 84 Cuts at: 1479 1734 Size: 255 Fragments arranged by size: 915 255 252 84 84 70 48 18 8 AflIII A'CryG_T Cuts at: 0 761 1734 Size: 761 973 AhdI GACnn_n'nnGTC Cuts at: 0 415 1734 Size: 415 1319 AleI CACnn'nnGTG Cuts at: 0 1706 1734 Size: 1706 28 AlwNI CAG_nnn'CTG Cuts at: 0 237 270 327 475 1734 Size: 237 33 57 148 1259 Fragments arranged by size: 1259 237 148 57 33 ApaI G_GGCC'C Cuts at: 0 222 1734 Size: 222 1512 ApoI r'AATT_y Cuts at: 0 750 1613 1734 Size: 750 863 121 Fragments arranged by size: 863 750 121 AvaI C'yCGr_G Cuts at: 0 194 905 1734 Size: 194 711 829 Fragments arranged by size: 829 711 194 BanII G_rGCy'C Cuts at: 0 222 320 1003 1423 1734 Size: 222 98 683 420 311 Fragments arranged by size: 683 420 311 222 98 BbsI GAAGACnn'nnnn_ Cuts at: 0 674 1734 Size: 674 1060 BbvCI CC'TCA_GC Cuts at: 0 238 1734 Size: 238 1496 Bme1580I G_kGCm'C Cuts at: 0 222 539 1734 Size: 222 317 1195 Fragments arranged by size: 1195 317 222 BmrI ACTGGGnnnn_n' Cuts at: 0 1043 1211 1379 1463 1734 Size: 1043 168 168 84 271 Fragments arranged by size: 1043 271 168 168 84 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 599 1734 Size: 599 1135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 304 307 515 626 1041 1559 1734 Size: 292 12 3 208 111 415 518 175 Fragments arranged by size: 518 415 292 208 175 111 12 3 Bpu10I CC'TnA_GC Cuts at: 0 238 1002 1734 Size: 238 764 732 Fragments arranged by size: 764 732 238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 1734 Size: 910 824 BsaI GGTCTCn'nnnn_ Cuts at: 0 602 775 902 1287 1600 1734 Size: 602 173 127 385 313 134 Fragments arranged by size: 602 385 313 173 134 127 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 347 377 751 781 1734 Size: 347 30 374 30 953 Fragments arranged by size: 953 374 347 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 97 328 373 469 709 1458 1734 Size: 97 231 45 96 240 749 276 Fragments arranged by size: 749 276 240 231 97 96 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 461 1168 1252 1734 Size: 461 707 84 482 Fragments arranged by size: 707 482 461 84 BsiHKAI G_wGCw'C Cuts at: 0 320 1003 1423 1734 Size: 320 683 420 311 Fragments arranged by size: 683 420 320 311 BsmBI CGTCTCn'nnnn_ Cuts at: 0 951 1734 Size: 951 783 Bsp1286I G_dGCh'C Cuts at: 0 222 320 539 1003 1423 1734 Size: 222 98 219 464 420 311 Fragments arranged by size: 464 420 311 222 219 98 BsrDI GCAATG_nn' Cuts at: 0 1482 1734 Size: 1482 252 BstEII G'GTnAC_C Cuts at: 0 366 1734 Size: 366 1368 BstXI CCAn_nnnn'nTGG Cuts at: 0 844 1734 Size: 844 890 BstYI r'GATC_y Cuts at: 0 60 266 723 1734 Size: 60 206 457 1011 Fragments arranged by size: 1011 457 206 60 Bsu36I CC'TnA_GG Cuts at: 0 311 1734 Size: 311 1423 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1212 1247 1464 1499 1734 Size: 1212 35 217 35 235 Fragments arranged by size: 1212 235 217 35 35 EaeI y'GGCC_r Cuts at: 0 135 1734 Size: 135 1599 EarI CTCTTCn'nnn_ Cuts at: 0 50 1734 Size: 50 1684 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 139 1431 1734 Size: 139 1292 303 Fragments arranged by size: 1292 303 139 EcoICRI GAG'CTC Cuts at: 0 318 1001 1421 1734 Size: 318 683 420 313 Fragments arranged by size: 683 420 318 313 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 8 26 74 144 292 304 307 515 Size: 8 18 48 70 148 12 3 208 Cuts at: 515 626 1041 1059 1143 1395 1479 1559 1734 Size: 111 415 18 84 252 84 80 175 Fragments arranged by size: 415 252 208 175 148 111 84 84 80 70 48 18 18 12 8 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 310 376 1734 Size: 310 66 1358 Fragments arranged by size: 1358 310 66 EcoO109I rG'GnC_Cy Cuts at: 0 190 219 1490 1734 Size: 190 29 1271 244 Fragments arranged by size: 1271 244 190 29 EcoRI G'AATT_C Cuts at: 0 1613 1734 Size: 1613 121 EcoRV GAT'ATC Cuts at: 0 1110 1734 Size: 1110 624 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 38 79 1734 Size: 38 41 1655 Fragments arranged by size: 1655 41 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 91 123 401 433 1410 1442 1662 1694 Size: 91 32 278 32 977 32 220 32 Cuts at: 1694 1734 Size: 40 Fragments arranged by size: 977 278 220 91 40 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 987 1322 1574 1734 Size: 987 335 252 160 Fragments arranged by size: 987 335 252 160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 234 1734 Size: 234 1500 MscI TGG'CCA Cuts at: 0 137 1734 Size: 137 1597 MslI CAynn'nnrTG Cuts at: 0 676 968 1218 1706 1734 Size: 676 292 250 488 28 Fragments arranged by size: 676 488 292 250 28 MspA1I CmG'CkG Cuts at: 0 856 936 1734 Size: 856 80 798 Fragments arranged by size: 856 798 80 NspI r_CATG'y Cuts at: 0 765 1734 Size: 765 969 PasI CC'CwG_GG Cuts at: 0 771 1734 Size: 771 963 PciI A'CATG_T Cuts at: 0 761 1734 Size: 761 973 PflMI CCAn_nnn'nTGG Cuts at: 0 327 654 1734 Size: 327 327 1080 Fragments arranged by size: 1080 327 327 PfoI T'CCnGG_A Cuts at: 0 269 1734 Size: 269 1465 PpuMI rG'GwC_Cy Cuts at: 0 190 1734 Size: 190 1544 PspOMI G'GGCC_C Cuts at: 0 218 1734 Size: 218 1516 PvuII CAG'CTG Cuts at: 0 856 1734 Size: 856 878 SacI G_AGCT'C Cuts at: 0 320 1003 1423 1734 Size: 320 683 420 311 Fragments arranged by size: 683 420 320 311 SmlI C'TyrA_G Cuts at: 0 889 1734 Size: 889 845 StuI AGG'CCT Cuts at: 0 1007 1175 1511 1734 Size: 1007 168 336 223 Fragments arranged by size: 1007 336 223 168 StyI C'CwwG_G Cuts at: 0 257 1734 Size: 257 1477 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 763 1734 Size: 763 971 TatI w'GTAC_w Cuts at: 0 462 1734 Size: 462 1272 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 347 379 1734 Size: 347 32 1355 Fragments arranged by size: 1355 347 32 XmnI GAAnn'nnTTC Cuts at: 0 21 1240 1324 1408 1576 1734 Size: 21 1219 84 84 168 158 Fragments arranged by size: 1219 168 158 84 84 21 Enzymes that do cut: AccI AcuI AflIII AhdI AleI AlwNI ApaI ApoI AvaI BanII BbsI BbvCI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BsrDI BstEII BstXI BstYI Bsu36I CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HindIII MmeI MscI MslI MspA1I NspI PasI PciI PflMI PfoI PpuMI PspOMI PvuII SacI SmlI StuI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EagI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI