Restriction map for
pFN21AE4276
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BcnI
BglI BlnI BlpI Bpu1102I BsaAI
BsiEI BsiWI BspDI BspHI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FseI
FspI HaeII HhaI HinP1I KasI
KpnI KspI MluI MspA1I MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 2221 GACGTC GACGT^C
AccI 2102 GTCTAC GT^[AC][GT]AC
AclI 2759 AACGTT AA^CGTT
AcyI 2221 GACGTC G[GA]^CG[CT]C
AflII 99 CTTAAG C^TTAAG
Alw44I 3299 GTGCAC G^TGCAC
ApaLI 3299 GTGCAC G^TGCAC
AspI 2540 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 497 CTCGAG C^[CT]CG[GA]G
BalI 2365 TGGCCA TGG^CCA
BanII 88 GGGCTC G[GA]GC[CT]^C
BbvCI 3557 CCTCAGC CCTCAGC(-5/-2)
BciVI 2048 GTATCC GTATCC(6/5)
BfrI 99 CTTAAG C^TTAAG
BsaHI 2221 GACGTC G[GA]^CG[CT]C
BsmBI 3420 GAGACG CGTCTC(1/5)
BsoBI 497 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2276 TGTACA T^GTACA
BspMI 1485 ACCTGC ACCTGC(4/8)
BsrDI 467 GCAATG GCAATG(2/0)
BsrGI 2276 TGTACA T^GTACA
BssSI 3601 CTCGTG CACGAG(-5/-1)
DsaI 3594 CCACGG C^C[GA][CT]GG
EaeI 2365 TGGCCA [CT]^GGCC[GA]
FauI 2478 GCGGG CCCGC(4/6)
HgaI 3178 GACGC GACGC(5/10)
Hin1I 2221 GACGTC G[GA]^CG[CT]C
HpaI 1288 GTTAAC GTT^AAC
MluNI 2365 TGGCCA TGG^CCA
MscI 2365 TGGCCA TGG^CCA
PaeR7I 497 CTCGAG C^TCGAG
PflFI 2540 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1711 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2759 AACGTT AA^CGTT
SapI 796 GCTCTTC GCTCTTC(1/4)
SphI 995 GCATGC GCATG^C
SspBI 2276 TGTACA T^GTACA
SwaI 1454 ATTTAAAT ATTT^AAAT
Tth111I 2540 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1711 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 707 CCACTAGAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 497 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccIII 123 TCCGGA T^CCGGA
1241 TCCGGA
AciI 1073 GCGG CCGC(-3/-1)
2478 GCGG
AflIII 170 ACATGT A^C[GA][CT]GT
2313 ACATGT
BclI 2706 TGATCA T^GATCA
3287 TGATCA
BglII 1651 AGATCT A^GATCT
2651 AGATCT
BsaBI 3583 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3700 GATGTGAATC
BsaI 3720 GAGACC GGTCTC(1/5)
3598 GGTCTC
BsaWI 123 TCCGGA [AT]^CCGG[AT]
1241 TCCGGA
BseAI 123 TCCGGA T^CCGGA
1241 TCCGGA
BsiYI 874 CCTCTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1711 CCATCCAGTGG
BslI 874 CCTCTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1711 CCATCCAGTGG
BsmFI 3344 GGGAC GGGAC(10/14)
2664 GTCCC
BspEI 123 TCCGGA T^CCGGA
1241 TCCGGA
BspLU11I 170 ACATGT A^CATGT
2313 ACATGT
BstAPI 961 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1305 GCAGCATCTGC
BstXI 2516 CCAAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3333 CCAGATTAGTGG
DraII 1575 AGGTCCT [GA]G^G[ACGT]CC[CT]
2662 GGGTCCC
EcoO109I 1575 AGGTCCT [GA]G^G[ACGT]CC[CT]
2662 GGGTCCC
EcoT22I 993 ATGCAT ATGCA^T
2891 ATGCAT
FbaI 2706 TGATCA T^GATCA
3287 TGATCA
HapII 124 CCGG C^CGG
1242 CCGG
HindIII 685 AAGCTT A^AGCTT
2159 AAGCTT
HpaII 124 CCGG C^CGG
1242 CCGG
MamI 3583 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3700 GATGTGAATC
MfeI 839 CAATTG C^AATTG
2425 CAATTG
MroI 123 TCCGGA T^CCGGA
1241 TCCGGA
MslI 1887 CATTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2314 CATGTAAATG
MspI 124 CCGG C^CGG
1242 CCGG
MunI 839 CAATTG C^AATTG
2425 CAATTG
NdeI 629 CATATG CA^TATG
3380 CATATG
NsiI 993 ATGCAT ATGCA^T
2891 ATGCAT
PpuMI 1575 AGGTCCT [GA]G^G[AT]CC[CT]
2662 GGGTCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AspHI AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsmAI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI