(Circular) (Six-base) MAPSORT of: pFN21AE4276.seq Check: 9789 from: 1 to: 8488 pFN21AE4276 8488 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4230 6298 278 Size: 53 83 186 936 2694 2068 2468 Fragments arranged by size: 2694 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4108 5831 1937 Size: 2171 1723 4594 Fragments arranged by size: 4594 2171 1723 Acc65I G'GTAC_C Cuts at: 5809 5809 Size: 8488 AclI AA'CG_TT Cuts at: 1563 4765 6615 6988 1563 Size: 3202 1850 373 3063 Fragments arranged by size: 3202 3063 1850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3444 3462 3759 4797 5418 6551 7935 8203 Size: 1812 18 297 1038 621 1133 1384 268 Cuts at: 8203 1632 Size: 1917 Fragments arranged by size: 1917 1812 1384 1133 1038 621 297 268 18 AflII C'TTAA_G Cuts at: 820 1017 2104 820 Size: 197 1087 7204 Fragments arranged by size: 7204 1087 197 AflIII A'CryG_T Cuts at: 1203 2175 4318 7387 8364 1203 Size: 972 2143 3069 977 1327 Fragments arranged by size: 3069 2143 1327 977 972 AgeI A'CCGG_T Cuts at: 7319 7319 Size: 8488 AhdI GACnn_n'nnGTC Cuts at: 7217 7217 Size: 8488 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8488 AlwNI CAG_nnn'CTG Cuts at: 2788 3316 3782 6231 7803 2788 Size: 528 466 2449 1572 3473 Fragments arranged by size: 3473 2449 1572 528 466 ApaLI G'TGCA_C Cuts at: 5304 6545 7701 5304 Size: 1241 1156 6091 Fragments arranged by size: 6091 1241 1156 ApoI r'AATT_y Cuts at: 1493 2566 2760 2867 3231 3398 3415 3958 4019 Size: 1073 194 107 364 167 17 543 61 Cuts at: 4019 4116 4202 4289 4398 4444 4830 5131 5207 Size: 97 86 87 109 46 386 301 76 Cuts at: 5207 5241 5772 5797 6023 6175 8284 1493 Size: 34 531 25 226 152 2109 1697 Fragments arranged by size: 2109 1697 1073 543 531 386 364 301 226 194 167 152 109 107 97 87 86 76 61 46 34 25 17 AseI AT'TA_AT Cuts at: 160 1032 7042 160 Size: 872 6010 1606 Fragments arranged by size: 6010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8488 AvaI C'yCGr_G Cuts at: 1870 1943 2502 5813 1870 Size: 73 559 3311 4545 Fragments arranged by size: 4545 3311 559 73 BamHI G'GATC_C Cuts at: 5818 6185 5818 Size: 367 8121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5809 7264 618 Size: 325 217 627 4022 1455 1842 Fragments arranged by size: 4022 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2097 5807 1391 Size: 706 3710 4072 Fragments arranged by size: 4072 3710 706 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3027 3060 4365 4448 5424 5685 5763 Size: 971 1128 33 1305 83 976 261 78 Cuts at: 5763 928 Size: 3653 Fragments arranged by size: 3653 1305 1128 976 971 261 83 78 33 BbvCI CC'TCA_GC Cuts at: 5563 5563 Size: 8488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6679 6713 1903 Size: 34 4742 34 3678 Fragments arranged by size: 4742 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4064 6380 7590 4064 Size: 2316 1210 4962 Fragments arranged by size: 4962 2316 1210 BclI T'GATC_A Cuts at: 1548 4711 5292 1548 Size: 3163 581 4744 Fragments arranged by size: 4744 3163 581 BfrBI ATG'CAT Cuts at: 3000 4898 3000 Size: 1898 6590 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7099 136 Size: 107 122 71 631 605 5427 1525 Fragments arranged by size: 5427 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3656 4656 8483 3656 Size: 1000 3827 3661 Fragments arranged by size: 3827 3661 1000 BlpI GC'TnA_GC Cuts at: 5905 5905 Size: 8488 Bme1580I G_kGCm'C Cuts at: 1790 5308 6549 7705 1790 Size: 3518 1241 1156 2573 Fragments arranged by size: 3518 2573 1241 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8488 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5295 5370 6219 Size: 416 53 83 399 24 3878 75 849 Cuts at: 6219 7177 442 Size: 958 1753 Fragments arranged by size: 3878 1753 958 849 416 399 83 75 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8488 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5049 5049 Size: 8488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2840 3657 5294 5321 7148 1239 Size: 1601 817 1637 27 1827 2579 Fragments arranged by size: 2579 1827 1637 1601 817 27 Bpu10I CC'TnA_GC Cuts at: 4980 5563 4980 Size: 583 7905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 6613 7478 7776 2081 Size: 4532 865 298 2793 Fragments arranged by size: 4532 2793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5609 5719 7151 882 Size: 617 4110 110 1432 2219 Fragments arranged by size: 4110 2219 1432 617 110 BsaAI yAC'GTr Cuts at: 493 1204 6279 493 Size: 711 5075 2702 Fragments arranged by size: 5075 2702 711 BsaBI GATnn'nnATC Cuts at: 5592 5709 8482 5592 Size: 117 2773 5598 Fragments arranged by size: 5598 2773 117 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4227 6295 6677 7312 Size: 53 83 186 936 2694 2068 382 635 Cuts at: 7312 275 Size: 1451 Fragments arranged by size: 2694 2068 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2128 3246 6921 7319 7593 7740 8151 2128 Size: 1118 3675 398 274 147 411 2465 Fragments arranged by size: 3675 2465 1118 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3050 3080 4561 4591 1178 Size: 30 165 30 1647 30 1481 30 5075 Fragments arranged by size: 5075 1647 1481 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3071 3146 4582 5558 5702 1186 Size: 1885 75 1436 976 144 3972 Fragments arranged by size: 3972 1885 1436 976 144 75 BseYI C'CCAG_C Cuts at: 7691 8187 7691 Size: 496 7992 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2894 3069 3588 1163 Size: 573 1158 175 519 6063 Fragments arranged by size: 6063 1158 573 519 175 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5938 6699 7727 1540 Size: 275 186 3937 761 1028 2301 Fragments arranged by size: 3937 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3635 5308 5467 6549 6634 7705 3635 Size: 1673 159 1082 85 1071 4418 Fragments arranged by size: 4418 1673 1082 1071 159 85 BsmI GAATG_Cn' Cuts at: 2977 4185 5748 5999 6092 2977 Size: 1208 1563 251 93 5373 Fragments arranged by size: 5373 1563 1208 251 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5419 5419 Size: 8488 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 3635 5308 5467 5807 6549 6634 Size: 399 307 1538 1673 159 340 742 85 Cuts at: 6634 7705 1391 Size: 1071 2174 Fragments arranged by size: 2174 1673 1538 1071 742 399 340 307 159 85 BspEI T'CCGG_A Cuts at: 2128 3246 8151 2128 Size: 1118 4905 2465 Fragments arranged by size: 4905 2465 1118 BspHI T'CATG_A Cuts at: 6377 8107 6377 Size: 1730 6758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3499 5843 844 Size: 2655 2344 3489 Fragments arranged by size: 3489 2655 2344 BsrBI CCG'CTC Cuts at: 1394 1823 6375 1394 Size: 429 4552 3507 Fragments arranged by size: 4552 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2479 6983 7157 65 Size: 1183 1231 4504 174 1396 Fragments arranged by size: 4504 1396 1231 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7132 7319 1700 Size: 110 5322 187 2869 Fragments arranged by size: 5322 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4281 96 Size: 4185 4303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8488 BssSI C'ACGA_G Cuts at: 5606 6548 7560 5606 Size: 942 1012 6534 Fragments arranged by size: 6534 1012 942 BstAPI GCAn_nnn'nTGC Cuts at: 794 2972 3316 794 Size: 2178 344 5966 Fragments arranged by size: 5966 2178 344 BstBI TT'CG_AA Cuts at: 7292 7292 Size: 8488 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8488 BstXI CCAn_nnnn'nTGG Cuts at: 4528 5345 4528 Size: 817 7671 BstYI r'GATC_y Cuts at: 1972 3656 3860 4311 4656 5818 6185 6570 6587 Size: 1684 204 451 345 1162 367 385 17 Cuts at: 6587 8039 8125 8137 8483 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1684 1452 1162 451 385 367 346 345 204 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 5599 513 Size: 552 4534 3402 Fragments arranged by size: 4534 3402 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7749 Fragments arranged by size: 7749 683 56 BtsI GCAGTG_nn' Cuts at: 2328 2777 5474 5577 6004 6798 6818 2328 Size: 449 2697 103 427 794 20 3998 Fragments arranged by size: 3998 2697 794 449 427 103 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5001 5036 529 Size: 35 4437 35 3981 Fragments arranged by size: 4437 3981 35 35 DraI TTT'AAA Cuts at: 2218 2262 3462 5792 2218 Size: 44 1200 2330 4914 Fragments arranged by size: 4914 2330 1200 44 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7495 809 Size: 507 6179 1802 Fragments arranged by size: 6179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4370 5935 6824 Size: 54 1425 50 275 18 2540 1565 889 Cuts at: 6824 8453 8 Size: 1629 43 Fragments arranged by size: 2540 1629 1565 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5935 1537 Size: 275 4123 4090 Fragments arranged by size: 4123 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2393 2808 3048 3123 3305 3540 3915 6418 Size: 1040 415 240 75 182 235 375 2503 Cuts at: 6418 1353 Size: 3423 Fragments arranged by size: 3423 2503 1040 415 375 240 235 182 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3093 7075 7449 7595 387 Size: 1064 138 1504 3982 374 146 1280 Fragments arranged by size: 3982 1504 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2840 3444 3462 3657 3759 4797 5294 Size: 393 1208 604 18 195 102 1038 497 Cuts at: 5294 5321 5418 6551 7148 7935 8203 1239 Size: 27 97 1133 597 787 268 1524 Fragments arranged by size: 1524 1208 1133 1038 787 604 597 497 393 268 195 102 97 27 18 EcoO109I rG'GnC_Cy Cuts at: 3581 4668 3581 Size: 1087 7401 EcoRI G'AATT_C Cuts at: 2760 4019 4398 5797 6175 2760 Size: 1259 379 1399 378 5073 Fragments arranged by size: 5073 1399 1259 379 378 EcoRV GAT'ATC Cuts at: 2158 4219 4804 2158 Size: 2061 585 5842 Fragments arranged by size: 5842 2061 585 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2713 2713 Size: 8488 FspI TGC'GCA Cuts at: 6994 6994 Size: 8488 HaeII r_GCGC'y Cuts at: 1678 7635 1678 Size: 5957 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3050 3082 3542 3574 3656 Size: 32 135 32 1478 32 460 32 82 Cuts at: 3656 3688 4536 4568 4928 4960 5414 5446 5624 Size: 32 848 32 360 32 454 32 178 Cuts at: 5624 5656 7129 7161 7203 7235 1373 Size: 32 1473 32 42 32 2626 Fragments arranged by size: 2626 1478 1473 848 460 454 360 178 135 82 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2060 3295 5010 5669 5832 6078 Size: 988 281 122 1235 1715 659 163 246 Cuts at: 6078 669 Size: 3079 Fragments arranged by size: 3079 1715 1235 988 659 281 246 163 122 HindIII A'AGCT_T Cuts at: 748 2690 4164 748 Size: 1942 1474 5072 Fragments arranged by size: 5072 1942 1474 HpaI GTT'AAC Cuts at: 3295 6078 3295 Size: 2783 5705 KpnI G_GTAC'C Cuts at: 5813 5813 Size: 8488 MfeI C'AATT_G Cuts at: 2844 4430 6087 2844 Size: 1586 1657 5245 Fragments arranged by size: 5245 1657 1586 MluI A'CGCG_T Cuts at: 8364 8364 Size: 8488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 3105 4339 7602 7786 1305 Size: 831 969 1234 3263 184 2007 Fragments arranged by size: 3263 2007 1234 969 831 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4372 10 Size: 54 1425 343 2540 4126 Fragments arranged by size: 4126 2540 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3896 4323 6446 6805 6964 518 Size: 648 2730 427 2123 359 159 2042 Fragments arranged by size: 2730 2123 2042 648 427 359 159 MspA1I CmG'CkG Cuts at: 1606 5904 6581 7729 7974 1606 Size: 4298 677 1148 245 2120 Fragments arranged by size: 4298 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7936 NdeI CA'TA_TG Cuts at: 387 2635 5386 387 Size: 2248 2751 3489 Fragments arranged by size: 3489 2751 2248 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8488 NotI GC'GGCC_GC Cuts at: 5935 5935 Size: 8488 NsiI A_TGCA'T Cuts at: 3002 4900 3002 Size: 1898 6590 NspI r_CATG'y Cuts at: 1132 1229 2179 3004 3310 4322 4902 5516 5846 Size: 97 950 825 306 1012 580 614 330 Cuts at: 5846 7391 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1012 950 825 614 580 330 306 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8488 PciI A'CATG_T Cuts at: 2175 4318 7387 2175 Size: 2143 3069 3276 Fragments arranged by size: 3276 3069 2143 PflMI CCAn_nnn'nTGG Cuts at: 1108 3722 1108 Size: 2614 5874 PfoI T'CCnGG_A Cuts at: 2391 3682 3991 5310 2391 Size: 1291 309 1319 5569 Fragments arranged by size: 5569 1319 1291 309 PmeI GTTT'AAAC Cuts at: 5792 5792 Size: 8488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2597 2629 6557 6589 8070 8102 2597 Size: 32 3928 32 1481 32 2983 Fragments arranged by size: 3928 2983 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3581 4668 3581 Size: 1087 7401 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8488 PsiI TTA'TAA Cuts at: 3593 5505 6058 3593 Size: 1912 553 6023 Fragments arranged by size: 6023 1912 553 PstI C_TGCA'G Cuts at: 830 2320 2784 3321 3742 5481 5840 830 Size: 1490 464 537 421 1739 359 3478 Fragments arranged by size: 3478 1739 1490 537 464 421 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8488 SalI G'TCGA_C Cuts at: 1936 5830 1936 Size: 3894 4594 SanDI GG'GwC_CC Cuts at: 4668 4668 Size: 8488 SapI GCTCTTCn'nnn_ Cuts at: 2808 2808 Size: 8488 SbfI CC_TGCA'GG Cuts at: 5840 5840 Size: 8488 ScaI AGT'ACT Cuts at: 6736 8448 6736 Size: 1712 6776 SfcI C'TryA_G Cuts at: 826 1045 2316 2780 3317 3738 4109 5067 5234 Size: 219 1271 464 537 421 371 958 167 Cuts at: 5234 5477 5836 6971 7652 7843 826 Size: 243 359 1135 681 191 1471 Fragments arranged by size: 1471 1271 1135 958 681 537 464 421 371 359 243 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8488 SmaI CCC'GGG Cuts at: 1872 5815 1872 Size: 3943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 2104 2502 6592 7493 7755 Size: 197 926 153 8 398 4090 901 262 Cuts at: 7755 820 Size: 1553 Fragments arranged by size: 4090 1553 926 901 398 262 197 153 8 SnaBI TAC'GTA Cuts at: 493 6279 493 Size: 5786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8488 SphI G_CATG'C Cuts at: 1132 3004 5846 1132 Size: 1872 2842 3774 Fragments arranged by size: 3774 2842 1872 SspI AAT'ATT Cuts at: 5 52 6412 5 Size: 47 6360 2081 Fragments arranged by size: 6360 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8488 StyI C'CwwG_G Cuts at: 513 1065 3796 3904 4562 5036 5729 5927 513 Size: 552 2731 108 658 474 693 198 3074 Fragments arranged by size: 3074 2731 693 658 552 474 198 108 SwaI ATTT'AAAT Cuts at: 3462 3462 Size: 8488 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5482 6515 6532 6685 6870 7315 325 Size: 1255 3902 1033 17 153 185 445 1498 Fragments arranged by size: 3902 1498 1255 1033 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3390 4281 4471 Size: 274 80 33 51 1443 1413 891 190 Cuts at: 4471 6734 8446 96 Size: 2263 1712 138 Fragments arranged by size: 2263 1712 1443 1413 891 274 190 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3243 3275 3604 3636 4561 4593 8448 8480 3243 Size: 32 329 32 925 32 3855 32 3251 Fragments arranged by size: 3855 3251 925 329 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4548 1316 Size: 3232 5256 XbaI T'CTAG_A Cuts at: 2239 3211 3653 3748 4927 5824 2239 Size: 972 442 95 1179 897 4903 Fragments arranged by size: 4903 1179 972 897 442 95 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2719 2719 Size: 8488 XhoI C'TCGA_G Cuts at: 1943 2502 1943 Size: 559 7929 XmaI C'CCGG_G Cuts at: 1870 5813 1870 Size: 3943 4545 XmnI GAAnn'nnTTC Cuts at: 2226 2569 4658 6617 2226 Size: 343 2089 1959 4097 Fragments arranged by size: 4097 2089 1959 343 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4228 6296 276 Size: 53 83 186 936 2694 2068 2468 Fragments arranged by size: 2694 2468 2068 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI