Restriction map for
pFN21AE4276
(Linear) (Six-base) MAPSORT of: KIEE4276.seq Check: 7004 from: 1 to: 3783
KIEE4276.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 0 2225 3783
Size: 2225 1558
AccI GT'mk_AC
Cuts at: 0 2103 3783
Size: 2103 1680
AclI AA'CG_TT
Cuts at: 0 2760 3783
Size: 2760 1023
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1439 1457 1754 2792 3413 3783
Size: 1439 18 297 1038 621 370
Fragments arranged by size:
1439 1038 621 370 297 18
AflII C'TTAA_G
Cuts at: 0 99 3783
Size: 99 3684
AflIII A'CryG_T
Cuts at: 0 170 2313 3783
Size: 170 2143 1470
Fragments arranged by size:
2143 1470 170
AlwNI CAG_nnn'CTG
Cuts at: 0 783 1311 1777 3783
Size: 783 528 466 2006
Fragments arranged by size:
2006 783 528 466
ApaLI G'TGCA_C
Cuts at: 0 3299 3783
Size: 3299 484
ApoI r'AATT_y
Cuts at: 0 561 755 862 1226 1393 1410 1953 2014
Size: 561 194 107 364 167 17 543 61
Cuts at: 2014 2111 2197 2284 2393 2439 2825 3126 3202
Size: 97 86 87 109 46 386 301 76
Cuts at: 3202 3236 3767 3783
Size: 34 531 16
Fragments arranged by size:
561 543 531 386 364 301 194 167
109 107 97 87 86 76 61 46
34 17 16
AvaI C'yCGr_G
Cuts at: 0 497 3783
Size: 497 3286
BanII G_rGCy'C
Cuts at: 0 92 3783
Size: 92 3691
BbsI GAAGACnn'nnnn_
Cuts at: 0 1022 1055 2360 2443 3419 3680 3758 3783
Size: 1022 33 1305 83 976 261 78 25
Fragments arranged by size:
1305 1022 976 261 83 78 33 25
BbvCI CC'TCA_GC
Cuts at: 0 3558 3783
Size: 3558 225
BciVI GTATCCnnnnn_n'
Cuts at: 0 2059 3783
Size: 2059 1724
BclI T'GATC_A
Cuts at: 0 2706 3287 3783
Size: 2706 581 496
Fragments arranged by size:
2706 581 496
BfrBI ATG'CAT
Cuts at: 0 995 2893 3783
Size: 995 1898 890
Fragments arranged by size:
1898 995 890
BglII A'GATC_T
Cuts at: 0 1651 2651 3783
Size: 1651 1000 1132
Fragments arranged by size:
1651 1132 1000
Bme1580I G_kGCm'C
Cuts at: 0 3303 3783
Size: 3303 480
BmrI ACTGGGnnnn_n'
Cuts at: 0 3290 3365 3783
Size: 3290 75 418
Fragments arranged by size:
3290 418 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3044 3783
Size: 3044 739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1652 3289 3316 3783
Size: 835 817 1637 27 467
Fragments arranged by size:
1637 835 817 467 27
Bpu10I CC'TnA_GC
Cuts at: 0 2975 3558 3783
Size: 2975 583 225
Fragments arranged by size:
2975 583 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 3783
Size: 76 3707
BsaI GGTCTCn'nnnn_
Cuts at: 0 3604 3714 3783
Size: 3604 110 69
Fragments arranged by size:
3604 110 69
BsaBI GATnn'nnATC
Cuts at: 0 3587 3704 3783
Size: 3587 117 79
Fragments arranged by size:
3587 117 79
BsaHI Gr'CG_yC
Cuts at: 0 2222 3783
Size: 2222 1561
BsaWI w'CCGG_w
Cuts at: 0 123 1241 3783
Size: 123 1118 2542
Fragments arranged by size:
2542 1118 123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1045 1075 2556 2586 3783
Size: 1045 30 1481 30 1197
Fragments arranged by size:
1481 1197 1045 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1066 1141 2577 3553 3697 3783
Size: 1066 75 1436 976 144 86
Fragments arranged by size:
1436 1066 976 144 86 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1064 1583 3783
Size: 889 175 519 2200
Fragments arranged by size:
2200 889 519 175
BsiHKAI G_wGCw'C
Cuts at: 0 1630 3303 3462 3783
Size: 1630 1673 159 321
Fragments arranged by size:
1673 1630 321 159
BsmI GAATG_Cn'
Cuts at: 0 972 2180 3743 3783
Size: 972 1208 1563 40
Fragments arranged by size:
1563 1208 972 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3414 3783
Size: 3414 369
Bsp1286I G_dGCh'C
Cuts at: 0 92 1630 3303 3462 3783
Size: 92 1538 1673 159 321
Fragments arranged by size:
1673 1538 321 159 92
BspEI T'CCGG_A
Cuts at: 0 123 1241 3783
Size: 123 1118 2542
Fragments arranged by size:
2542 1118 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1494 3783
Size: 1494 2289
BsrDI GCAATG_nn'
Cuts at: 0 474 3783
Size: 474 3309
BsrGI T'GTAC_A
Cuts at: 0 2276 3783
Size: 2276 1507
BssSI C'ACGA_G
Cuts at: 0 3601 3783
Size: 3601 182
BstAPI GCAn_nnn'nTGC
Cuts at: 0 967 1311 3783
Size: 967 344 2472
Fragments arranged by size:
2472 967 344
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2523 3340 3783
Size: 2523 817 443
Fragments arranged by size:
2523 817 443
BstYI r'GATC_y
Cuts at: 0 1651 1855 2306 2651 3783
Size: 1651 204 451 345 1132
Fragments arranged by size:
1651 1132 451 345 204
BtgI C'CryG_G
Cuts at: 0 3594 3783
Size: 3594 189
BtsI GCAGTG_nn'
Cuts at: 0 323 772 3469 3572 3783
Size: 323 449 2697 103 211
Fragments arranged by size:
2697 449 323 211 103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2996 3031 3783
Size: 2996 35 752
Fragments arranged by size:
2996 752 35
DraI TTT'AAA
Cuts at: 0 213 257 1457 3783
Size: 213 44 1200 2326
Fragments arranged by size:
2326 1200 213 44
EaeI y'GGCC_r
Cuts at: 0 2365 3783
Size: 2365 1418
EarI CTCTTCn'nnn_
Cuts at: 0 388 803 1043 1118 1300 1535 1910 3783
Size: 388 415 240 75 182 235 375 1873
Fragments arranged by size:
1873 415 388 375 240 235 182 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1088 3783
Size: 1088 2695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1439 1457 1652 1754 2792 3289 3316
Size: 835 604 18 195 102 1038 497 27
Cuts at: 3316 3413 3783
Size: 97 370
Fragments arranged by size:
1038 835 604 497 370 195 102 97
27 18
EcoO109I rG'GnC_Cy
Cuts at: 0 1576 2663 3783
Size: 1576 1087 1120
Fragments arranged by size:
1576 1120 1087
EcoRI G'AATT_C
Cuts at: 0 755 2014 2393 3783
Size: 755 1259 379 1390
Fragments arranged by size:
1390 1259 755 379
EcoRV GAT'ATC
Cuts at: 0 153 2214 2799 3783
Size: 153 2061 585 984
Fragments arranged by size:
2061 984 585 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 708 3783
Size: 708 3075
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1537 1569 1651 1683 2531 2563
Size: 1045 32 460 32 82 32 848 32
Cuts at: 2563 2923 2955 3409 3441 3619 3651 3783
Size: 360 32 454 32 178 32 132
Fragments arranged by size:
1045 848 460 454 360 178 132 82
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 55 1290 3005 3664 3783
Size: 55 1235 1715 659 119
Fragments arranged by size:
1715 1235 659 119 55
HindIII A'AGCT_T
Cuts at: 0 685 2159 3783
Size: 685 1474 1624
Fragments arranged by size:
1624 1474 685
HpaI GTT'AAC
Cuts at: 0 1290 3783
Size: 1290 2493
MfeI C'AATT_G
Cuts at: 0 839 2425 3783
Size: 839 1586 1358
Fragments arranged by size:
1586 1358 839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1100 2334 3783
Size: 131 969 1234 1449
Fragments arranged by size:
1449 1234 969 131
MscI TGG'CCA
Cuts at: 0 2367 3783
Size: 2367 1416
MslI CAynn'nnrTG
Cuts at: 0 1891 2318 3783
Size: 1891 427 1465
Fragments arranged by size:
1891 1465 427
NdeI CA'TA_TG
Cuts at: 0 630 3381 3783
Size: 630 2751 402
Fragments arranged by size:
2751 630 402
NsiI A_TGCA'T
Cuts at: 0 997 2895 3783
Size: 997 1898 888
Fragments arranged by size:
1898 997 888
NspI r_CATG'y
Cuts at: 0 174 999 1305 2317 2897 3511 3783
Size: 174 825 306 1012 580 614 272
Fragments arranged by size:
1012 825 614 580 306 272 174
PciI A'CATG_T
Cuts at: 0 170 2313 3783
Size: 170 2143 1470
Fragments arranged by size:
2143 1470 170
PflMI CCAn_nnn'nTGG
Cuts at: 0 1717 3783
Size: 1717 2066
PfoI T'CCnGG_A
Cuts at: 0 386 1677 1986 3305 3783
Size: 386 1291 309 1319 478
Fragments arranged by size:
1319 1291 478 386 309
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 3783
Size: 592 32 3159
Fragments arranged by size:
3159 592 32
PpuMI rG'GwC_Cy
Cuts at: 0 1576 2663 3783
Size: 1576 1087 1120
Fragments arranged by size:
1576 1120 1087
PsiI TTA'TAA
Cuts at: 0 1588 3500 3783
Size: 1588 1912 283
Fragments arranged by size:
1912 1588 283
PstI C_TGCA'G
Cuts at: 0 315 779 1316 1737 3476 3783
Size: 315 464 537 421 1739 307
Fragments arranged by size:
1739 537 464 421 315 307
SanDI GG'GwC_CC
Cuts at: 0 2663 3783
Size: 2663 1120
SapI GCTCTTCn'nnn_
Cuts at: 0 803 3783
Size: 803 2980
SfcI C'TryA_G
Cuts at: 0 311 775 1312 1733 2104 3062 3229 3472
Size: 311 464 537 421 371 958 167 243
Cuts at: 3472 3783
Size: 311
Fragments arranged by size:
958 537 464 421 371 311 311 243
167
SmlI C'TyrA_G
Cuts at: 0 91 99 497 3783
Size: 91 8 398 3286
Fragments arranged by size:
3286 398 91 8
SphI G_CATG'C
Cuts at: 0 999 3783
Size: 999 2784
StyI C'CwwG_G
Cuts at: 0 1791 1899 2557 3031 3724 3783
Size: 1791 108 658 474 693 59
Fragments arranged by size:
1791 693 658 474 108 59
SwaI ATTT'AAAT
Cuts at: 0 1457 3783
Size: 1457 2326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3477 3783
Size: 3477 306
TatI w'GTAC_w
Cuts at: 0 1385 2276 2466 3783
Size: 1385 891 190 1317
Fragments arranged by size:
1385 1317 891 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1238 1270 1599 1631 2556 2588 3783
Size: 1238 32 329 32 925 32 1195
Fragments arranged by size:
1238 1195 925 329 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2543 3783
Size: 2543 1240
XbaI T'CTAG_A
Cuts at: 0 234 1206 1648 1743 2922 3783
Size: 234 972 442 95 1179 861
Fragments arranged by size:
1179 972 861 442 234 95
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 714 3783
Size: 714 3069
XhoI C'TCGA_G
Cuts at: 0 497 3783
Size: 497 3286
XmnI GAAnn'nnTTC
Cuts at: 0 221 564 2653 3783
Size: 221 343 2089 1130
Fragments arranged by size:
2089 1130 343 221
ZraI GAC'GTC
Cuts at: 0 2223 3783
Size: 2223 1560
Enzymes that do cut:
AatII AccI AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AvaI BanII BbsI BbvCI BciVI BclI BfrBI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrDI BsrGI BssSI BstAPI
BstXI BstYI BtgI BtsI CspCI DraI EaeI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI NdeI NsiI
NspI PciI PflMI PfoI PpiI PpuMI PsiI PstI
SanDI SapI SfcI SmlI SphI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BcgI BglI BlpI BmgBI BmtI BsaAI BseYI
BsiEI BsiWI BspHI BsrBI BsrFI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI DraIII DrdI EagI EcoICRI
EcoNI FseI FspI FspAI HaeII KasI KpnI MluI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PmeI PmlI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI XmaI