Restriction map for
pFN21AB5914
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbsI BbvCI BcgI
BciVI BfrI BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoT14I EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MluI MroI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI StyI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 674 GGTACC G^GTACC
AccI 109 GTAGAC GT^[AC][GT]AC
AcsI 1115 GAATTC [GA]^AATT[CT]
AcyI 781 GACGCC G[GA]^CG[CT]C
AlwI 1209 GATCC GGATC(4/5)
ApoI 1115 GAATTC [GA]^AATT[CT]
Asp700I 1111 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 674 GGTACC G^GTACC
BalI 238 TGGCCA TGG^CCA
BbrPI 1492 CACGTG CAC^GTG
BglII 1074 AGATCT A^GATCT
BsaAI 1492 CACGTG [CT]AC^GT[GA]
BsaHI 781 GACGCC G[GA]^CG[CT]C
BseRI 1338 CTCCTC GAGGAG(10/8)
Bsp1407I 571 TGTACA T^GTACA
BspDI 577 ATCGAT AT^CGAT
BspMI 1466 ACCTGC ACCTGC(4/8)
BsrBI 1329 GAGCGG CCGCTC(-3/-3)
BsrDI 179 GCAATG GCAATG(2/0)
BsrGI 571 TGTACA T^GTACA
BstXI 1355 CCATGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1074 AGATCT [GA]^GATC[CT]
ClaI 577 ATCGAT AT^CGAT
DraI 517 TTTAAA TTT^AAA
EcoNI 813 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1115 GAATTC G^AATTC
EcoRV 473 GATATC GAT^ATC
HaeII 1034 GGCGCT [GA]GCGC^[CT]
HgaI 781 GACGC GACGC(5/10)
Hin1I 781 GACGCC G[GA]^CG[CT]C
KpnI 674 GGTACC GGTAC^C
MboII 942 GAAGA GAAGA(8/7)
MfeI 1040 CAATTG C^AATTG
MflI 1074 AGATCT [GA]^GATC[CT]
MluNI 238 TGGCCA TGG^CCA
MscI 238 TGGCCA TGG^CCA
MunI 1040 CAATTG C^AATTG
NaeI 724 GCCGGC GCC^GGC
NgoMIV 724 GCCGGC G^CCGGC
PflMI 241 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1492 CACGTG CAC^GTG
PmlI 1492 CACGTG CAC^GTG
SacI 961 GAGCTC GAGCT^C
SphI 1398 GCATGC GCATG^C
SspBI 571 TGTACA T^GTACA
TfiI 945 GATTC G^A[AT]TC
Van91I 241 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1074 AGATCT [GA]^GATC[CT]
XmnI 1111 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 753 CGCG CG^CG
1501 CGCG
AvaI 758 CCCGAG C^[CT]CG[GA]G
793 CCCGAG
BanI 251 GGCACC G^G[CT][GA]CC
674 GGTACC
BclI 842 TGATCA T^GATCA
926 TGATCA
BglI 204 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
724 GCCGGCAAGGC
BlpI 823 GCTGAGC GC^T[ACGT]AGC
963 GCTCAGC
Bpu1102I 823 GCTGAGC GC^T[ACGT]AGC
963 GCTCAGC
BsgI 715 CTGCAC GTGCAG(16/14)
1533 GTGCAG
BsoBI 758 CCCGAG C^[CT]CG[GA]G
793 CCCGAG
BsrFI 587 ACCGGC [GA]^CCGG[CT]
724 GCCGGC
BstUI 753 CGCG CG^CG
1501 CGCG
CelII 823 GCTGAGC GC^T[ACGT]AGC
963 GCTCAGC
Cfr10I 587 ACCGGC [GA]^CCGG[CT]
724 GCCGGC
FauI 765 GCGGG CCCGC(4/6)
1502 GCGGG
FbaI 842 TGATCA T^GATCA
926 TGATCA
MaeII 1391 ACGT A^CGT
1493 ACGT
MslI 289 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
879 CATGATGATG
MspA1I 712 CAGCTG C[AC]G^C[GT]G
930 CAGCTG
MvnI 753 CGCG CG^CG
1501 CGCG
NspI 1133 ACATGC [GA]CATG^[CT]
1398 GCATGC
PleI 312 GAGTC GAGTC(4/5)
485 GAGTC
PstI 51 CTGCAG CTGCA^G
453 CTGCAG
PvuII 712 CAGCTG CAG^CTG
930 CAGCTG
XcmI 62 CCATCTTACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
226 CCAAATGTGTTGTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I