(Circular) (Six-base) MAPSORT of: pFN21AB5914.seq Check: 1708 from: 1 to: 6295 pFN21AB5914 6295 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2115 3638 1937 Size: 178 1523 4594 Fragments arranged by size: 4594 1523 178 Acc65I G'GTAC_C Cuts at: 2679 3616 2679 Size: 937 5358 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4358 5742 6010 1632 Size: 2726 1384 268 1917 Fragments arranged by size: 2726 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 5194 6171 1203 Size: 3991 977 1327 Fragments arranged by size: 3991 1327 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6295 AlwNI CAG_nnn'CTG Cuts at: 4038 5610 4038 Size: 1572 4723 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 3120 3604 3830 3982 6091 1493 Size: 1627 484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1627 484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 2763 2798 3620 1870 Size: 73 820 35 822 4545 Fragments arranged by size: 4545 822 820 73 35 BamHI G'GATC_C Cuts at: 3625 3992 3625 Size: 367 5928 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2256 2679 3616 5071 618 Size: 325 217 627 469 423 937 1455 1842 Fragments arranged by size: 1842 1455 937 627 469 423 325 217 BanII G_rGCy'C Cuts at: 1391 2798 2970 3575 3614 1391 Size: 1407 172 605 39 4072 Fragments arranged by size: 4072 1407 605 172 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5324 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4486 4520 1903 Size: 34 2549 34 3678 Fragments arranged by size: 3678 2549 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4187 5397 4187 Size: 1210 5085 BclI T'GATC_A Cuts at: 1548 2847 2931 1548 Size: 1299 84 4912 Fragments arranged by size: 4912 1299 84 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2215 2735 4906 Size: 107 122 71 631 605 543 520 2171 Cuts at: 4906 136 Size: 1525 Fragments arranged by size: 2171 1525 631 605 543 520 122 107 71 BglII A'GATC_T Cuts at: 3079 6290 3079 Size: 3211 3084 BlpI GC'TnA_GC Cuts at: 2829 2969 3712 2829 Size: 140 743 5412 Fragments arranged by size: 5412 743 140 Bme1580I G_kGCm'C Cuts at: 1790 2259 4356 5512 1790 Size: 469 2097 1156 2573 Fragments arranged by size: 2573 2097 1156 469 BmgBI CAC'GTC Cuts at: 1318 3397 1318 Size: 2079 4216 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2853 3455 4026 Size: 416 53 83 399 24 1436 602 571 Cuts at: 4026 4984 442 Size: 958 1753 Fragments arranged by size: 1753 1436 958 602 571 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2457 2104 Size: 353 5942 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2843 3060 3500 4955 1239 Size: 860 744 217 440 1455 2579 Fragments arranged by size: 2579 1455 860 744 440 217 Bpu10I CC'TnA_GC Cuts at: 2792 2792 Size: 6295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4420 5285 5583 4420 Size: 865 298 5132 Fragments arranged by size: 5132 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4958 882 Size: 617 3459 2219 Fragments arranged by size: 3459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3499 4086 493 Size: 711 2295 587 2702 Fragments arranged by size: 2702 2295 711 587 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2787 4102 4484 5119 Size: 53 83 186 936 1254 1315 382 635 Cuts at: 5119 275 Size: 1451 Fragments arranged by size: 1451 1315 1254 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6070 Fragments arranged by size: 6070 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3334 1186 Size: 2148 4147 BseYI C'CCAG_C Cuts at: 2180 2937 3348 5498 5994 2180 Size: 757 411 2150 496 2481 Fragments arranged by size: 2481 2150 757 496 411 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2705 3559 1163 Size: 573 969 854 3899 Fragments arranged by size: 3899 969 854 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2814 2970 4356 4441 5512 2085 Size: 729 156 1386 85 1071 2868 Fragments arranged by size: 2868 1386 1071 729 156 85 BsmI GAATG_Cn' Cuts at: 2175 2532 3496 3806 3899 2175 Size: 357 964 310 93 4571 Fragments arranged by size: 4571 964 357 310 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2259 2798 2814 2970 3575 3614 Size: 399 295 174 539 16 156 605 39 Cuts at: 3614 4356 4441 5512 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 605 539 399 295 174 156 85 39 16 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3480 3650 844 Size: 2636 170 3489 Fragments arranged by size: 3489 2636 170 BsrBI CCG'CTC Cuts at: 1394 1823 3336 4182 1394 Size: 429 1513 846 3507 Fragments arranged by size: 3507 1513 846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2191 4790 4964 65 Size: 1183 943 2599 174 1396 Fragments arranged by size: 2599 1396 1183 943 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2592 2729 4939 5126 1700 Size: 110 782 137 2210 187 2869 Fragments arranged by size: 2869 2210 782 187 137 110 BsrGI T'GTAC_A Cuts at: 96 2576 96 Size: 2480 3815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 2604 2809 3361 794 Size: 1810 205 552 3728 Fragments arranged by size: 3728 1810 552 205 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6295 BstXI CCAn_nnnn'nTGG Cuts at: 3367 3367 Size: 6295 BstYI r'GATC_y Cuts at: 1972 3079 3625 3992 4377 4394 5846 5932 5944 Size: 1107 546 367 385 17 1452 86 12 Cuts at: 5944 6290 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1107 546 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4804 Fragments arranged by size: 4804 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2264 487 Size: 683 56 1038 4518 Fragments arranged by size: 4518 1038 683 56 BtsI GCAGTG_nn' Cuts at: 3811 4605 4625 3811 Size: 794 20 5481 Fragments arranged by size: 5481 794 20 ClaI AT'CG_AT Cuts at: 1554 2583 1554 Size: 1029 5266 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2728 2763 529 Size: 35 2164 35 4061 Fragments arranged by size: 4061 2164 35 35 DraI TTT'AAA Cuts at: 2524 3599 2524 Size: 1075 5220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2243 2752 2907 Size: 54 1425 50 275 18 413 509 155 Cuts at: 2907 3742 4631 6260 8 Size: 835 889 1629 43 Fragments arranged by size: 1629 1425 889 835 509 413 275 155 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4225 1353 Size: 2872 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2968 2968 Size: 6295 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2843 3060 3500 4358 4955 5742 Size: 393 467 744 217 440 858 597 787 Cuts at: 5742 6010 1239 Size: 268 1524 Fragments arranged by size: 1524 858 787 744 597 467 440 393 268 217 EcoNI CCTnn'n_nnAGG Cuts at: 2822 2822 Size: 6295 EcoRI G'AATT_C Cuts at: 3120 3604 3982 3120 Size: 484 378 5433 Fragments arranged by size: 5433 484 378 EcoRV GAT'ATC Cuts at: 2480 2480 Size: 6295 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3085 3085 Size: 6295 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 3043 5442 1678 Size: 1365 2399 2531 Fragments arranged by size: 2531 2399 1365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2456 2488 4936 4968 5010 Size: 32 135 32 884 32 2448 32 42 Cuts at: 5010 5042 1373 Size: 32 2626 Fragments arranged by size: 2626 2448 884 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3639 3885 669 Size: 988 281 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6295 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 2683 3620 2683 Size: 937 5358 MfeI C'AATT_G Cuts at: 3045 3894 3045 Size: 849 5446 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2987 3205 5409 5593 1305 Size: 1682 218 2204 184 2007 Fragments arranged by size: 2204 2007 1682 218 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2245 10 Size: 54 1425 343 413 4060 Fragments arranged by size: 4060 1425 413 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 2888 4253 4612 4771 518 Size: 648 1132 590 1365 359 159 2042 Fragments arranged by size: 2042 1365 1132 648 590 359 159 MspA1I CmG'CkG Cuts at: 1606 2719 2937 3711 4388 5536 5781 1606 Size: 1113 218 774 677 1148 245 2120 Fragments arranged by size: 2120 1148 1113 774 677 245 218 NaeI GCC'GGC Cuts at: 2731 2731 Size: 6295 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4804 Fragments arranged by size: 4804 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NgoMIV G'CCGG_C Cuts at: 2729 2729 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 3142 3407 3653 5198 1132 Size: 97 1913 265 246 1545 2229 Fragments arranged by size: 2229 1913 1545 265 246 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6295 PciI A'CATG_T Cuts at: 5194 5194 Size: 6295 PflMI CCAn_nnn'nTGG Cuts at: 1108 2252 1108 Size: 1144 5151 PfoI T'CCnGG_A Cuts at: 2660 2820 2660 Size: 160 6135 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PmlI CAC'GTG Cuts at: 3499 3499 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3417 3449 4364 4396 5877 5909 3417 Size: 32 915 32 1481 32 3803 Fragments arranged by size: 3803 1481 915 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2337 2369 2337 Size: 32 6263 PstI C_TGCA'G Cuts at: 830 2060 2462 3647 830 Size: 1230 402 1185 3478 Fragments arranged by size: 3478 1230 1185 402 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 PvuII CAG'CTG Cuts at: 2719 2937 2719 Size: 218 6077 SacI G_AGCT'C Cuts at: 2970 2970 Size: 6295 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SfcI C'TryA_G Cuts at: 826 1045 2056 2149 2458 3643 4778 5459 5650 Size: 219 1011 93 309 1185 1135 681 191 Cuts at: 5650 826 Size: 1471 Fragments arranged by size: 1471 1185 1135 1011 681 309 219 191 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4399 5300 5562 820 Size: 197 926 2456 901 262 1553 Fragments arranged by size: 2456 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 3407 3653 1132 Size: 2275 246 3774 Fragments arranged by size: 3774 2275 246 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 2516 2612 2879 1517 Size: 999 96 267 4933 Fragments arranged by size: 4933 999 267 96 StyI C'CwwG_G Cuts at: 513 1065 2004 3734 513 Size: 552 939 1730 3074 Fragments arranged by size: 3074 1730 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3310 3320 4322 4339 4492 4677 5122 Size: 1255 1730 10 1002 17 153 185 445 Cuts at: 5122 325 Size: 1498 Fragments arranged by size: 1730 1498 1255 1002 445 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2576 4541 6253 Size: 274 80 33 51 1443 599 1965 1712 Cuts at: 6253 96 Size: 138 Fragments arranged by size: 1965 1712 1443 599 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2374 2406 6255 6287 2374 Size: 32 3849 32 2382 Fragments arranged by size: 3849 2382 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2238 2074 Size: 164 6131 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 3120 4424 3120 Size: 1304 4991 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI