Restriction map for
pFN21AB5914
(Linear) (Six-base) MAPSORT of: KIBB5914.seq Check: 529 from: 1 to: 1590
KIBB5914.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 0 110 1590
Size: 110 1480
Acc65I G'GTAC_C
Cuts at: 0 674 1590
Size: 674 916
ApoI r'AATT_y
Cuts at: 0 1115 1590
Size: 1115 475
AvaI C'yCGr_G
Cuts at: 0 758 793 1590
Size: 758 35 797
Fragments arranged by size:
797 758 35
BanI G'GyrC_C
Cuts at: 0 251 674 1590
Size: 251 423 916
Fragments arranged by size:
916 423 251
BanII G_rGCy'C
Cuts at: 0 793 965 1570 1590
Size: 793 172 605 20
Fragments arranged by size:
793 605 172 20
BclI T'GATC_A
Cuts at: 0 842 926 1590
Size: 842 84 664
Fragments arranged by size:
842 664 84
BglI GCCn_nnn'nGGC
Cuts at: 0 210 730 1590
Size: 210 520 860
Fragments arranged by size:
860 520 210
BglII A'GATC_T
Cuts at: 0 1074 1590
Size: 1074 516
BlpI GC'TnA_GC
Cuts at: 0 824 964 1590
Size: 824 140 626
Fragments arranged by size:
824 626 140
Bme1580I G_kGCm'C
Cuts at: 0 254 1590
Size: 254 1336
BmgBI CAC'GTC
Cuts at: 0 1392 1590
Size: 1392 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 848 1450 1590
Size: 848 602 140
Fragments arranged by size:
848 602 140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 452 1590
Size: 99 353 1138
Fragments arranged by size:
1138 353 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 838 1055 1495 1590
Size: 94 744 217 440 95
Fragments arranged by size:
744 440 217 95 94
Bpu10I CC'TnA_GC
Cuts at: 0 787 1590
Size: 787 803
BsaAI yAC'GTr
Cuts at: 0 1494 1590
Size: 1494 96
BsaHI Gr'CG_yC
Cuts at: 0 782 1590
Size: 782 808
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1329 1590
Size: 1329 261
BseYI C'CCAG_C
Cuts at: 0 175 932 1343 1590
Size: 175 757 411 247
Fragments arranged by size:
757 411 247 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1554 1590
Size: 700 854 36
Fragments arranged by size:
854 700 36
BsiHKAI G_wGCw'C
Cuts at: 0 80 809 965 1590
Size: 80 729 156 625
Fragments arranged by size:
729 625 156 80
BsmI GAATG_Cn'
Cuts at: 0 170 527 1491 1590
Size: 170 357 964 99
Fragments arranged by size:
964 357 170 99
Bsp1286I G_dGCh'C
Cuts at: 0 80 254 793 809 965 1570 1590
Size: 80 174 539 16 156 605 20
Fragments arranged by size:
605 539 174 156 80 20 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1475 1590
Size: 1475 115
BsrBI CCG'CTC
Cuts at: 0 1331 1590
Size: 1331 259
BsrDI GCAATG_nn'
Cuts at: 0 186 1590
Size: 186 1404
BsrFI r'CCGG_y
Cuts at: 0 587 724 1590
Size: 587 137 866
Fragments arranged by size:
866 587 137
BsrGI T'GTAC_A
Cuts at: 0 571 1590
Size: 571 1019
BstAPI GCAn_nnn'nTGC
Cuts at: 0 599 804 1356 1590
Size: 599 205 552 234
Fragments arranged by size:
599 552 234 205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1362 1590
Size: 1362 228
BstYI r'GATC_y
Cuts at: 0 1074 1590
Size: 1074 516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 259 1590
Size: 259 1331
ClaI AT'CG_AT
Cuts at: 0 578 1590
Size: 578 1012
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 723 758 1590
Size: 723 35 832
Fragments arranged by size:
832 723 35
DraI TTT'AAA
Cuts at: 0 519 1590
Size: 519 1071
EaeI y'GGCC_r
Cuts at: 0 238 747 902 1590
Size: 238 509 155 688
Fragments arranged by size:
688 509 238 155
EcoICRI GAG'CTC
Cuts at: 0 963 1590
Size: 963 627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 838 1055 1495 1590
Size: 94 744 217 440 95
Fragments arranged by size:
744 440 217 95 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 817 1590
Size: 817 773
EcoRI G'AATT_C
Cuts at: 0 1115 1590
Size: 1115 475
EcoRV GAT'ATC
Cuts at: 0 475 1590
Size: 475 1115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1080 1590
Size: 1080 510
HaeII r_GCGC'y
Cuts at: 0 1038 1590
Size: 1038 552
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 1590
Size: 451 32 1107
Fragments arranged by size:
1107 451 32
KpnI G_GTAC'C
Cuts at: 0 678 1590
Size: 678 912
MfeI C'AATT_G
Cuts at: 0 1040 1590
Size: 1040 550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 1200 1590
Size: 982 218 390
Fragments arranged by size:
982 390 218
MscI TGG'CCA
Cuts at: 0 240 1590
Size: 240 1350
MslI CAynn'nnrTG
Cuts at: 0 293 883 1590
Size: 293 590 707
Fragments arranged by size:
707 590 293
MspA1I CmG'CkG
Cuts at: 0 714 932 1590
Size: 714 218 658
Fragments arranged by size:
714 658 218
NaeI GCC'GGC
Cuts at: 0 726 1590
Size: 726 864
NgoMIV G'CCGG_C
Cuts at: 0 724 1590
Size: 724 866
NspI r_CATG'y
Cuts at: 0 1137 1402 1590
Size: 1137 265 188
Fragments arranged by size:
1137 265 188
PflMI CCAn_nnn'nTGG
Cuts at: 0 247 1590
Size: 247 1343
PfoI T'CCnGG_A
Cuts at: 0 655 815 1590
Size: 655 160 775
Fragments arranged by size:
775 655 160
PmlI CAC'GTG
Cuts at: 0 1494 1590
Size: 1494 96
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1412 1444 1590
Size: 1412 32 146
Fragments arranged by size:
1412 146 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 332 364 1590
Size: 332 32 1226
Fragments arranged by size:
1226 332 32
PstI C_TGCA'G
Cuts at: 0 55 457 1590
Size: 55 402 1133
Fragments arranged by size:
1133 402 55
PvuII CAG'CTG
Cuts at: 0 714 932 1590
Size: 714 218 658
Fragments arranged by size:
714 658 218
SacI G_AGCT'C
Cuts at: 0 965 1590
Size: 965 625
SfcI C'TryA_G
Cuts at: 0 51 144 453 1590
Size: 51 93 309 1137
Fragments arranged by size:
1137 309 93 51
SphI G_CATG'C
Cuts at: 0 1402 1590
Size: 1402 188
StuI AGG'CCT
Cuts at: 0 511 607 874 1590
Size: 511 96 267 716
Fragments arranged by size:
716 511 267 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1305 1315 1590
Size: 1305 10 275
Fragments arranged by size:
1305 275 10
TatI w'GTAC_w
Cuts at: 0 571 1590
Size: 571 1019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 369 401 1590
Size: 369 32 1189
Fragments arranged by size:
1189 369 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 233 1590
Size: 69 164 1357
Fragments arranged by size:
1357 164 69
XmnI GAAnn'nnTTC
Cuts at: 0 1115 1590
Size: 1115 475
Enzymes that do cut:
AccI Acc65I ApoI AvaI BanI BanII BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
BtgZI ClaI CspCI DraI EaeI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI HaeII Hin4I KpnI MfeI MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PflMI PfoI
PmlI PpiI PsrI PstI PvuII SacI SfcI SphI
StuI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BfrBI BmtI BpuEI BsaI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgI BtsI DraIII DrdI
EagI EarI EciI EcoO109I FseI FspI FspAI HincII
HindIII HpaI KasI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PpuMI
PshAI PsiI PspOMI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI
SwaI Tth111I XbaI XhoI XmaI ZraI