################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccI 121 GTCTAC GT^[AC][GT]AC AflIII 91 ACGTGT A^C[GA][CT]GT Aor51HI 230 AGCGCT AGC^GCT AspHI 517 GTGCTC G[AT]GC[AT]^C BcnI 299 CCCGG CC^[GC]GG BlpI 14 GCTCAGC GC^T[ACGT]AGC Bpu1102I 14 GCTCAGC GC^T[ACGT]AGC BsiHKAI 517 GTGCTC G[AT]GC[AT]^C BsmFI 212 GGGAC GGGAC(10/14) BspHI 336 TCATGA T^CATGA BspMI 204 GCAGGT ACCTGC(4/8) BssHII 541 GCGCGC G^CGCGC CelII 14 GCTCAGC GC^T[ACGT]AGC DpnII 537 GATC ^GATC Eco47III 230 AGCGCT AGC^GCT EcoNI 420 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 230 AGCGCT [GA]GCGC^[CT] HapII 300 CCGG C^CGG HpaII 300 CCGG C^CGG MaeIII 395 GTGAC ^GT[ACGT]AC MboI 537 GATC ^GATC MslI 556 CACCTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 218 CAGCTG C[AC]G^C[GT]G MspI 300 CCGG C^CGG NciI 299 CCCGG CC^[GC]GG NdeII 537 GATC ^GATC NlaIV 621 GGGTCC GG[ACGT]^[ACGT]CC PvuII 218 CAGCTG CAG^CTG RcaI 336 TCATGA T^CATGA Sau3AI 537 GATC ^GATC Tsp45I 395 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 540 CGCG CG^CG 542 CGCG AvaII 305 GGTCC G^G[AT]CC 622 GGTCC BalI 222 TGGCCA TGG^CCA 524 TGGCCA BbsI 87 GAAGAC GAAGAC(2/6) 511 GTCTTC BpuAI 87 GAAGAC GAAGAC(2/6) 511 GTCTTC BsmAI 130 GAGAC GTCTC(1/5) 475 GAGAC BsrI 329 ACTGG ACTGG(1/-1) 441 ACTGG BstF5I 321 CATCC GGATG(2/0) 429 GGATG BstUI 540 CGCG CG^CG 542 CGCG EcoT14I 485 CCAAGG C^C[AT][AT]GG 527 CCAAGG FokI 321 CATCC GGATG(9/13) 429 GGATG HphI 245 TCACC GGTGA(8/7) 296 TCACC MluNI 222 TGGCCA TGG^CCA 524 TGGCCA MscI 222 TGGCCA TGG^CCA 524 TGGCCA MvnI 540 CGCG CG^CG 542 CGCG NlaIII 337 CATG CATG^ 522 CATG SfaNI 201 GATGC GCATC(5/9) 430 GATGC StyI 485 CCAAGG C^C[AT][AT]GG 527 CCAAGG TaqI 314 TCGA T^CGA 425 TCGA TfiI 73 GATTC G^A[AT]TC 333 GATTC TthHB8I 314 TCGA T^CGA 425 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI BbvI BmyI BpmI BsaJI BsgI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI EaeI EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MaeII MboII MnlI MvaI MwoI PleI Sau96I ScrFI TseI TspRI