################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 911 CGCG CG^CG ApaI 736 GGGCCC GGGCC^C BalI 93 TGGCCA TGG^CCA BanI 893 GGTGCC G^G[CT][GA]CC BfaI 880 CTAG C^TAG BsaAI 42 TACGTG [CT]AC^GT[GA] BsmBI 622 GAGACG CGTCTC(1/5) BspMI 218 ACCTGC ACCTGC(4/8) BsrI 1057 ACTGG ACTGG(1/-1) BssSI 307 CACGAG CACGAG(-5/-1) BstUI 911 CGCG CG^CG DpnII 468 GATC ^GATC DraIII 388 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 242 CCAAGG C^C[AT][AT]GG HaeII 949 GGCGCT [GA]GCGC^[CT] HindIII 319 AAGCTT A^AGCTT MaeI 880 CTAG C^TAG MaeIII 686 GTCAC ^GT[ACGT]AC MboI 468 GATC ^GATC MluNI 93 TGGCCA TGG^CCA MscI 93 TGGCCA TGG^CCA MslI 348 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 911 CGCG CG^CG NdeII 468 GATC ^GATC PflMI 612 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 367 GAGTC GAGTC(4/5) Sau3AI 468 GATC ^GATC SexAI 584 ACCTGGT A^CC[AT]GGT SmaI 371 CCCGGG CCC^GGG SmlI 316 CTCAAG C^T[CT][GA]AG StyI 242 CCAAGG C^C[AT][AT]GG TfiI 483 GAATC G^A[AT]TC Tsp45I 686 GTCAC ^GT[GC]AC Van91I 612 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 879 TCTAGA T^CTAGA XmaCI 371 CCCGGG C^CCGGG XmaI 371 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AvaI 187 CCCGAG C^[CT]CG[GA]G 371 CCCGGG BlpI 123 GCTGAGC GC^T[ACGT]AGC 509 GCTTAGC Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC 509 GCTTAGC BsmAI 622 GAGAC GTCTC(1/5) 637 GAGAC BsmFI 204 GTCCC GGGAC(10/14) 369 GTCCC BsoBI 187 CCCGAG C^[CT]CG[GA]G 371 CCCGGG BsrBI 538 GAGCGG CCGCTC(-3/-3) 721 GAGCGG BstAPI 378 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 387 GCACAGGGTGC BstXI 50 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 347 CCATCTGTGTGG CelII 123 GCTGAGC GC^T[ACGT]AGC 509 GCTTAGC CfoI 259 GCGC GCG^C 950 GCGC DraII 735 GGGGCCC [GA]G^G[ACGT]CC[CT] 736 GGGCCCC EaeI 93 TGGCCA [CT]^GGCC[GA] 248 TGGCCG EcoNI 291 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1042 CCTCTGGGAGG EcoO109I 735 GGGGCCC [GA]G^G[ACGT]CC[CT] 736 GGGCCCC FauI 207 CCCGC CCCGC(4/6) 400 CCCGC HhaI 259 GCGC GCG^C 950 GCGC HinP1I 259 GCGC G^CGC 950 GCGC HinfI 367 GAGTC G^A[ACGT]TC 483 GAATC HphI 531 GGTGA GGTGA(8/7) 470 TCACC MaeII 43 ACGT A^CGT 788 ACGT MspA1I 121 CAGCTG C[AC]G^C[GT]G 712 CAGCTG NlaIII 447 CATG CATG^ 759 CATG PvuII 121 CAGCTG CAG^CTG 712 CAGCTG SapI 595 GAAGAGC GCTCTTC(1/4) 961 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI Ksp632I MboII MnlI MspI MvaI MwoI NciI NlaIV PstI RsaI Sau96I ScrFI SfaNI SfcI TseI TspRI