(Circular) (Six-base) MAPSORT of: pFN21AE3208.seq Check: 1124 from: 1 to: 5776 pFN21AE3208 5776 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3839 5223 5491 1632 Size: 2207 1384 268 1917 Fragments arranged by size: 2207 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2081 2623 2755 3519 5091 2081 Size: 542 132 764 1572 2766 Fragments arranged by size: 2766 1572 764 542 132 ApaI G_GGCC'C Cuts at: 2745 2745 Size: 5776 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2192 2376 3101 1870 Size: 73 249 184 725 4545 Fragments arranged by size: 4545 725 249 184 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2898 3097 4552 618 Size: 325 217 627 1111 199 1455 1842 Fragments arranged by size: 1842 1455 1111 627 325 217 199 BanII G_rGCy'C Cuts at: 1391 2180 2319 2351 2745 3095 1391 Size: 789 139 32 394 350 4072 Fragments arranged by size: 4072 789 394 350 139 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2236 2261 Size: 107 122 71 631 605 337 227 25 Cuts at: 2261 2414 4387 136 Size: 153 1973 1525 Fragments arranged by size: 1973 1525 631 605 337 227 153 122 107 71 25 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 2129 2515 3193 2129 Size: 386 678 4712 Fragments arranged by size: 4712 678 386 Bme1580I G_kGCm'C Cuts at: 1790 2745 2810 3837 4993 1790 Size: 955 65 1027 1156 2573 Fragments arranged by size: 2573 1156 1027 955 65 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3072 3507 4465 Size: 416 53 83 399 24 1655 435 958 Cuts at: 4465 442 Size: 1753 Fragments arranged by size: 1753 1655 958 435 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 2585 2600 2645 2720 2732 2741 4436 Size: 1223 123 15 45 75 12 9 1695 Cuts at: 4436 1239 Size: 2579 Fragments arranged by size: 2579 1695 1223 123 75 45 15 12 9 Bpu10I CC'TnA_GC Cuts at: 2777 2777 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2306 3901 4766 5064 2306 Size: 1595 865 298 3018 Fragments arranged by size: 3018 1595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2049 3567 493 Size: 711 845 1518 2702 Fragments arranged by size: 2702 1518 845 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5551 Fragments arranged by size: 5551 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 2459 2519 2603 2654 2738 2920 3036 Size: 1240 33 60 84 51 84 182 116 Cuts at: 3036 1186 Size: 3926 Fragments arranged by size: 3926 1240 182 116 84 84 60 51 33 BseYI C'CCAG_C Cuts at: 2133 4979 5475 2133 Size: 2846 496 2434 Fragments arranged by size: 2846 2434 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 2375 2386 2441 2822 2866 1163 Size: 573 482 157 11 55 381 44 4073 Fragments arranged by size: 4073 573 482 381 157 55 44 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2054 2310 2385 2735 3837 3922 4993 2054 Size: 256 75 350 1102 85 1071 2837 Fragments arranged by size: 2837 1102 1071 350 256 85 75 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 BsmBI CGTCTCn'nnnn_ Cuts at: 2621 2621 Size: 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2180 2310 2319 2351 2385 2735 Size: 399 264 126 130 9 32 34 350 Cuts at: 2735 2745 2810 3095 3837 3922 4993 1391 Size: 10 65 285 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 285 264 130 126 85 65 34 32 10 9 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2232 3131 844 Size: 1388 899 3489 Fragments arranged by size: 3489 1388 899 BsrBI CCG'CTC Cuts at: 1394 1823 2545 2728 3663 1394 Size: 429 722 183 935 3507 Fragments arranged by size: 3507 935 722 429 183 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 2385 2682 4420 4607 1700 Size: 110 425 150 297 1738 187 2869 Fragments arranged by size: 2869 1738 425 297 187 150 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 2312 3836 4848 2312 Size: 1524 1012 3240 Fragments arranged by size: 3240 1524 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2389 2398 794 Size: 1595 9 4172 Fragments arranged by size: 4172 1595 9 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2359 2062 Size: 297 5479 BstYI r'GATC_y Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771 Size: 1134 367 385 17 1452 86 12 346 Cuts at: 5771 1972 Size: 1977 Fragments arranged by size: 1977 1452 1134 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2424 2810 2988 3292 4086 4106 2424 Size: 386 178 304 794 20 4094 Fragments arranged by size: 4094 794 386 304 178 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DraIII CAC_nnn'GTG Cuts at: 2398 2398 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2253 3223 Size: 54 1425 50 275 18 268 155 970 Cuts at: 3223 4112 5741 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 970 889 275 268 155 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2595 2631 2973 3706 1353 Size: 1242 36 342 733 3423 Fragments arranged by size: 3423 1242 733 342 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2179 4363 4737 4883 387 Size: 1064 138 590 2184 374 146 1280 Fragments arranged by size: 2184 1280 1064 590 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2462 2585 2600 2645 2720 2732 2741 Size: 393 830 123 15 45 75 12 9 Cuts at: 2741 3839 4436 5223 5491 1239 Size: 1098 597 787 268 1524 Fragments arranged by size: 1524 1098 830 787 597 393 268 123 75 45 15 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 2300 3051 2300 Size: 751 5025 EcoO109I rG'GnC_Cy Cuts at: 2741 2742 2741 Size: 1 5775 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2197 2197 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2958 4923 1678 Size: 1280 1965 2531 Fragments arranged by size: 2531 1965 1280 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 2473 2505 2896 Size: 32 135 32 534 32 335 32 391 Cuts at: 2896 2928 4417 4449 4491 4523 1373 Size: 32 1489 32 42 32 2626 Fragments arranged by size: 2626 1489 534 391 335 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 2324 748 Size: 1576 4200 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 10 Size: 54 1425 343 268 3686 Fragments arranged by size: 3686 1425 343 268 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 3734 4093 4252 518 Size: 648 1191 1377 359 159 2042 Fragments arranged by size: 2042 1377 1191 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2719 3192 3869 5017 5262 1606 Size: 522 591 473 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 591 522 473 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 1108 Size: 1515 4261 PfoI T'CCnGG_A Cuts at: 2658 2854 2658 Size: 196 5580 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2741 2741 Size: 5776 PstI C_TGCA'G Cuts at: 830 2037 2286 2496 2718 2760 2995 3128 830 Size: 1207 249 210 222 42 235 133 3478 Fragments arranged by size: 3478 1207 249 235 222 210 133 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2128 2719 2128 Size: 591 5185 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2595 2973 2595 Size: 378 5398 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SexAI A'CCwGG_T Cuts at: 2589 2589 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 2033 2282 2492 2714 2756 2991 3124 Size: 219 988 249 210 222 42 235 133 Cuts at: 3124 4259 4940 5131 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 988 681 249 235 222 219 210 191 133 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 2378 3103 1872 Size: 506 725 4545 Fragments arranged by size: 4545 725 506 SmlI C'TyrA_G Cuts at: 820 1017 1943 2321 3880 4781 5043 820 Size: 197 926 378 1559 901 262 1553 Fragments arranged by size: 1559 1553 926 901 378 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 3215 513 Size: 552 939 243 968 3074 Fragments arranged by size: 3074 968 939 552 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3017 3803 3820 3973 4158 4603 325 Size: 1255 1437 786 17 153 185 445 1498 Fragments arranged by size: 1498 1437 1255 786 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2033 2065 5736 5768 2033 Size: 32 3671 32 2041 Fragments arranged by size: 3671 2041 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 2884 3112 2884 Size: 228 5548 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 2376 3101 1870 Size: 506 725 4545 Fragments arranged by size: 4545 725 506 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI