Restriction map for
pFN21AE3208
(Linear) (Six-base) MAPSORT of: KIEE3208.seq Check: 7669 from: 1 to: 1071
KIEE3208.orf
With 182 enzymes: SgfI *
July 3, 2012 17:26 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 76 618 750 1071
Size: 76 542 132 321
Fragments arranged by size:
542 321 132 76
ApaI G_GGCC'C
Cuts at: 0 740 1071
Size: 740 331
AvaI C'yCGr_G
Cuts at: 0 187 371 1071
Size: 187 184 700
Fragments arranged by size:
700 187 184
BanI G'GyrC_C
Cuts at: 0 893 1071
Size: 893 178
BanII G_rGCy'C
Cuts at: 0 175 314 346 740 1071
Size: 175 139 32 394 331
Fragments arranged by size:
394 331 175 139 32
BglI GCCn_nnn'nGGC
Cuts at: 0 231 256 409 1071
Size: 231 25 153 662
Fragments arranged by size:
662 231 153 25
BlpI GC'TnA_GC
Cuts at: 0 124 510 1071
Size: 124 386 561
Fragments arranged by size:
561 386 124
Bme1580I G_kGCm'C
Cuts at: 0 740 805 1071
Size: 740 65 266
Fragments arranged by size:
740 266 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 1067 1071
Size: 1067 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 580 595 640 715 727 736 1071
Size: 457 123 15 45 75 12 9 335
Fragments arranged by size:
457 335 123 75 45 15 12 9
Bpu10I CC'TnA_GC
Cuts at: 0 772 1071
Size: 772 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1071
Size: 301 770
BsaAI yAC'GTr
Cuts at: 0 44 1071
Size: 44 1027
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 454 514 598 649 733 915 1031
Size: 421 33 60 84 51 84 182 116
Cuts at: 1031 1071
Size: 40
Fragments arranged by size:
421 182 116 84 84 60 51 40
33
BseYI C'CCAG_C
Cuts at: 0 128 1071
Size: 128 943
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 370 381 436 817 861 1071
Size: 213 157 11 55 381 44 210
Fragments arranged by size:
381 213 210 157 55 44 11
BsiHKAI G_wGCw'C
Cuts at: 0 49 305 380 730 1071
Size: 49 256 75 350 341
Fragments arranged by size:
350 341 256 75 49
BsmBI CGTCTCn'nnnn_
Cuts at: 0 616 1071
Size: 616 455
Bsp1286I G_dGCh'C
Cuts at: 0 49 175 305 314 346 380 730 740
Size: 49 126 130 9 32 34 350 10
Cuts at: 740 805 1071
Size: 65 266
Fragments arranged by size:
350 266 130 126 65 49 34 32
10 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 1071
Size: 227 844
BsrBI CCG'CTC
Cuts at: 0 540 723 1071
Size: 540 183 348
Fragments arranged by size:
540 348 183
BsrFI r'CCGG_y
Cuts at: 0 230 380 677 1071
Size: 230 150 297 394
Fragments arranged by size:
394 297 230 150
BssSI C'ACGA_G
Cuts at: 0 307 1071
Size: 307 764
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 393 1071
Size: 384 9 678
Fragments arranged by size:
678 384 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 354 1071
Size: 57 297 717
Fragments arranged by size:
717 297 57
BtsI GCAGTG_nn'
Cuts at: 0 419 805 983 1071
Size: 419 386 178 88
Fragments arranged by size:
419 386 178 88
DraIII CAC_nnn'GTG
Cuts at: 0 393 1071
Size: 393 678
EaeI y'GGCC_r
Cuts at: 0 93 248 1071
Size: 93 155 823
Fragments arranged by size:
823 155 93
EarI CTCTTCn'nnn_
Cuts at: 0 590 626 968 1071
Size: 590 36 342 103
Fragments arranged by size:
590 342 103 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 174 1071
Size: 174 897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 580 595 640 715 727 736 1071
Size: 457 123 15 45 75 12 9 335
Fragments arranged by size:
457 335 123 75 45 15 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 1046 1071
Size: 295 751 25
Fragments arranged by size:
751 295 25
EcoO109I rG'GnC_Cy
Cuts at: 0 736 737 1071
Size: 736 1 334
Fragments arranged by size:
736 334 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 192 1071
Size: 192 879
HaeII r_GCGC'y
Cuts at: 0 953 1071
Size: 953 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 468 500 891 923 1071
Size: 101 32 335 32 391 32 148
Fragments arranged by size:
391 335 148 101 32 32 32
HindIII A'AGCT_T
Cuts at: 0 319 1071
Size: 319 752
MscI TGG'CCA
Cuts at: 0 95 1071
Size: 95 976
MslI CAynn'nnrTG
Cuts at: 0 352 1071
Size: 352 719
MspA1I CmG'CkG
Cuts at: 0 123 714 1071
Size: 123 591 357
Fragments arranged by size:
591 357 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 618 1071
Size: 618 453
PfoI T'CCnGG_A
Cuts at: 0 653 849 1071
Size: 653 196 222
Fragments arranged by size:
653 222 196
PspOMI G'GGCC_C
Cuts at: 0 736 1071
Size: 736 335
PstI C_TGCA'G
Cuts at: 0 32 281 491 713 755 990 1071
Size: 32 249 210 222 42 235 81
Fragments arranged by size:
249 235 222 210 81 42 32
PvuII CAG'CTG
Cuts at: 0 123 714 1071
Size: 123 591 357
Fragments arranged by size:
591 357 123
SapI GCTCTTCn'nnn_
Cuts at: 0 590 968 1071
Size: 590 378 103
Fragments arranged by size:
590 378 103
SexAI A'CCwGG_T
Cuts at: 0 584 1071
Size: 584 487
SfcI C'TryA_G
Cuts at: 0 28 277 487 709 751 986 1071
Size: 28 249 210 222 42 235 85
Fragments arranged by size:
249 235 222 210 85 42 28
SmaI CCC'GGG
Cuts at: 0 373 1071
Size: 373 698
SmlI C'TyrA_G
Cuts at: 0 316 1071
Size: 316 755
StyI C'CwwG_G
Cuts at: 0 242 1071
Size: 242 829
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1012 1071
Size: 1012 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 1071
Size: 28 32 1011
Fragments arranged by size:
1011 32 28
XbaI T'CTAG_A
Cuts at: 0 879 1071
Size: 879 192
XmaI C'CCGG_G
Cuts at: 0 371 1071
Size: 371 700
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaAI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI
BstXI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HindIII MscI MslI MspA1I
PflMI PfoI PspOMI PstI PvuII SapI SexAI SfcI
SmaI SmlI StyI TaqII TstI XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XcmI XhoI XmnI ZraI