################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeI MaeII MluI MluNI MroI MscI MspA1I NarI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI TaqI TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 176 CGCG CG^CG AciI 177 GCGG CCGC(-3/-1) AcsI 3 GAATTC [GA]^AATT[CT] AfaI 117 GTAC GT^AC AlwNI 495 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 817 GGGCCC GGGCC^C ApoI 3 GAATTC [GA]^AATT[CT] AspI 70 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 397 GCTGAGG CCTCAGC(-5/-2) BclI 1275 TGATCA T^GATCA BcnI 408 CCGGG CC^[GC]GG BpmI 733 CTCCAG CTGGAG(16/14) BsaBI 1270 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 665 CCAGGG C^C[ACGT][ACGT]GG BsaWI 362 ACCGGA [AT]^CCGG[AT] BsgI 812 GTGCAG GTGCAG(16/14) BsmI 160 GAATGC GAATGC(1/-1) BsrDI 225 CATTGC GCAATG(2/0) BsrFI 104 GCCGGC [GA]^CCGG[CT] BstAPI 139 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 176 CGCG CG^CG BstXI 735 CCAGGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 693 GGATCT [GA]^GATC[CT] Cfr10I 104 GCCGGC [GA]^CCGG[CT] DraII 816 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 816 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 3 GAATTC G^AATTC EcoRV 958 GATATC GAT^ATC FbaI 1275 TGATCA T^GATCA HindIII 449 AAGCTT A^AGCTT MamI 1270 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1305 CAATTG C^AATTG MflI 693 GGATCT [GA]^GATC[CT] MunI 1305 CAATTG C^AATTG MvnI 176 CGCG CG^CG NaeI 104 GCCGGC GCC^GGC NciI 408 CCGGG CC^[GC]GG NdeI 1036 CATATG CA^TATG NgoMIV 104 GCCGGC G^CCGGC NlaIV 817 GGGCCC GG[ACGT]^[ACGT]CC PflFI 70 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 117 GTAC GT^AC SapI 577 GAAGAGC GCTCTTC(1/4) SmlI 944 CTCAAG C^T[CT][GA]AG SspI 1019 AATATT AAT^ATT StuI 1330 AGGCCT AGG^CCT Tth111I 70 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 693 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AlwI 952 GATCC GGATC(4/5) 693 GGATC Asp700I 300 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 976 GAATGTTTTC BbsI 168 GAAGAC GAAGAC(2/6) 22 GTCTTC BpuAI 168 GAAGAC GAAGAC(2/6) 22 GTCTTC EarI 577 GAAGAG CTCTTC(1/4) 1092 GAAGAG Ksp632I 577 GAAGAG CTCTTC(1/4) 1092 GAAGAG MslI 48 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 757 CACAGGTATG PflMI 383 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 494 CCAGATGCTGG PstI 871 CTGCAG CTGCA^G 1164 CTGCAG SacI 415 GAGCTC GAGCT^C 805 GAGCTC Van91I 383 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 494 CCAGATGCTGG XmnI 300 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 976 GAATGTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AluI AspHI BanII BbvI BmyI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII PleI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI