(Circular) (Six-base) MAPSORT of: pFN21AE4088.seq Check: 8052 from: 1 to: 6061 pFN21AE4088 6061 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 4188 4561 1563 Size: 2625 373 3063 Fragments arranged by size: 3063 2625 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2723 4124 5508 5776 1632 Size: 1091 1401 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1091 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6061 AlwNI CAG_nnn'CTG Cuts at: 2505 3804 5376 2505 Size: 1299 1572 3190 Fragments arranged by size: 3190 1572 1299 ApaI G_GGCC'C Cuts at: 2826 2826 Size: 6061 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 2008 3370 3596 3748 5857 1493 Size: 515 1362 226 152 2109 1697 Fragments arranged by size: 2109 1697 1362 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 3386 1870 Size: 73 1443 4545 Fragments arranged by size: 4545 1443 73 BamHI G'GATC_C Cuts at: 3391 3758 3391 Size: 367 5694 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3382 4837 618 Size: 325 217 627 1595 1455 1842 Fragments arranged by size: 1842 1595 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2424 2814 2826 3004 3380 1391 Size: 1033 390 12 178 376 4072 Fragments arranged by size: 4072 1033 390 376 178 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2020 2180 928 Size: 971 121 160 4809 Fragments arranged by size: 4809 971 160 121 BbvCI CC'TCA_GC Cuts at: 2403 2403 Size: 6061 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4252 4286 1903 Size: 34 2315 34 3678 Fragments arranged by size: 3678 2315 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 3280 1548 Size: 1732 4329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4672 136 Size: 107 122 71 631 605 3000 1525 Fragments arranged by size: 3000 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 3478 3478 Size: 6061 Bme1580I G_kGCm'C Cuts at: 1790 2041 2100 2826 4122 5278 1790 Size: 251 59 726 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 726 251 59 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2072 3792 4750 Size: 416 53 83 399 24 655 1720 958 Cuts at: 4750 442 Size: 1753 Fragments arranged by size: 1753 1720 958 655 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2723 4721 1239 Size: 1484 1998 2579 Fragments arranged by size: 2579 1998 1484 Bpu10I CC'TnA_GC Cuts at: 2403 3003 2403 Size: 600 5461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2934 4186 5051 5349 2934 Size: 1252 865 298 3646 Fragments arranged by size: 3646 1252 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4724 882 Size: 617 3225 2219 Fragments arranged by size: 3225 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3852 493 Size: 711 2648 2702 Fragments arranged by size: 2702 2648 711 BsaBI GATnn'nnATC Cuts at: 3279 6055 3279 Size: 2776 3285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3868 4250 4885 275 Size: 53 83 186 936 2335 382 635 1451 Fragments arranged by size: 2335 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2367 4494 4892 5166 5313 5724 2367 Size: 2127 398 274 147 411 2704 Fragments arranged by size: 2704 2127 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5836 Fragments arranged by size: 5836 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2432 3140 3318 1186 Size: 1099 147 708 178 3929 Fragments arranged by size: 3929 1099 708 178 147 BseYI C'CCAG_C Cuts at: 5264 5760 5264 Size: 496 5565 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2838 1163 Size: 573 1102 4386 Fragments arranged by size: 4386 1102 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2162 2351 2367 2424 2814 4122 4207 5278 2162 Size: 189 16 57 390 1308 85 1071 2945 Fragments arranged by size: 2945 1308 1071 390 189 85 57 16 BsmI GAATG_Cn' Cuts at: 2171 3572 3665 2171 Size: 1401 93 4567 Fragments arranged by size: 4567 1401 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2100 2162 2351 2367 2424 2814 Size: 399 251 59 62 189 16 57 390 Cuts at: 2814 2826 3004 3380 4122 4207 5278 1391 Size: 12 178 376 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 376 251 189 178 85 62 59 57 16 12 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3416 844 Size: 2572 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3948 1394 Size: 429 2125 3507 Fragments arranged by size: 3507 2125 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2229 4556 4730 65 Size: 1183 981 2327 174 1396 Fragments arranged by size: 2327 1396 1183 981 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 4705 4892 1700 Size: 110 299 2596 187 2869 Fragments arranged by size: 2869 2596 299 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 4121 5133 4121 Size: 1012 5049 BstAPI GCAn_nnn'nTGC Cuts at: 794 2150 794 Size: 1356 4705 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2747 2747 Size: 6061 BstYI r'GATC_y Cuts at: 1972 2698 3391 3758 4143 4160 5612 5698 5710 Size: 726 693 367 385 17 1452 86 12 Cuts at: 5710 6056 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 726 693 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5509 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5322 Fragments arranged by size: 5322 683 56 BtsI GCAGTG_nn' Cuts at: 3166 3577 4371 4391 3166 Size: 411 794 20 4836 Fragments arranged by size: 4836 794 411 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6026 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026 Size: 54 1425 50 275 18 1678 889 1629 Cuts at: 6026 8 Size: 43 Fragments arranged by size: 1678 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 3092 3991 1353 Size: 1224 515 899 3423 Fragments arranged by size: 3423 1224 899 515 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4648 5022 5168 387 Size: 1064 138 3059 374 146 1280 Fragments arranged by size: 3059 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2422 2812 2422 Size: 390 5671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2723 4124 4721 5508 5776 1239 Size: 393 1091 1401 597 787 268 1524 Fragments arranged by size: 1524 1401 1091 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2822 2822 Size: 6061 EcoRI G'AATT_C Cuts at: 2008 3370 3748 2008 Size: 1362 378 4321 Fragments arranged by size: 4321 1362 378 EcoRV GAT'ATC Cuts at: 2965 2965 Size: 6061 FspI TGC'GCA Cuts at: 4567 4567 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 5208 1678 Size: 3530 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373 Size: 32 135 32 3130 32 42 32 2626 Fragments arranged by size: 3130 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 2454 748 Size: 1706 4355 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3310 3660 3310 Size: 350 5711 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 2917 5175 5359 1305 Size: 1071 541 2258 184 2007 Fragments arranged by size: 2258 2007 1071 541 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4239 Fragments arranged by size: 4239 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2766 4019 4378 4537 518 Size: 648 891 709 1253 359 159 2042 Fragments arranged by size: 2042 1253 891 709 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3477 4154 5302 5547 1606 Size: 1871 677 1148 245 2120 Fragments arranged by size: 2120 1871 1148 677 245 NaeI GCC'GGC Cuts at: 2111 2111 Size: 6061 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5509 NdeI CA'TA_TG Cuts at: 387 3042 387 Size: 2655 3406 NgoMIV G'CCGG_C Cuts at: 2109 2109 Size: 6061 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 3419 4964 1132 Size: 97 2190 1545 2229 Fragments arranged by size: 2229 2190 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6061 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2394 2505 1108 Size: 1286 111 4664 Fragments arranged by size: 4664 1286 111 PfoI T'CCnGG_A Cuts at: 3240 3240 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 2822 2822 Size: 6061 PstI C_TGCA'G Cuts at: 830 2880 3173 3413 830 Size: 2050 293 240 3478 Fragments arranged by size: 3478 2050 293 240 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 SacI G_AGCT'C Cuts at: 2424 2814 2424 Size: 390 5671 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SapI GCTCTTCn'nnn_ Cuts at: 2577 2577 Size: 6061 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 2876 3169 3349 3409 4544 5225 5416 Size: 219 1831 293 180 60 1135 681 191 Cuts at: 5416 826 Size: 1471 Fragments arranged by size: 1831 1471 1135 681 293 219 191 180 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3388 1872 Size: 1516 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2949 4165 5066 5328 820 Size: 197 926 1006 1216 901 262 1553 Fragments arranged by size: 1553 1216 1006 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3026 3985 5 Size: 47 2974 959 2081 Fragments arranged by size: 2974 2081 959 47 StuI AGG'CCT Cuts at: 1517 3337 1517 Size: 1820 4241 StyI C'CwwG_G Cuts at: 513 1065 3500 513 Size: 552 2435 3074 Fragments arranged by size: 3074 2435 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4088 4105 4258 4443 4888 325 Size: 1255 2508 17 153 185 445 1498 Fragments arranged by size: 2508 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4307 6019 96 Size: 274 80 33 51 1443 2330 1712 138 Fragments arranged by size: 2330 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6021 6053 6021 Size: 32 6029 Tth111I GACn'n_nGTC Cuts at: 1316 2078 1316 Size: 762 5299 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6061 XmaI C'CCGG_G Cuts at: 1870 3386 1870 Size: 1516 4545 XmnI GAAnn'nnTTC Cuts at: 2309 2985 4190 2309 Size: 676 1205 4180 Fragments arranged by size: 4180 1205 676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI