################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 721 GTCTAC GT^[AC][GT]AC AccII 835 CGCG CG^CG AfaI 1162 GTAC GT^AC AlwNI 1117 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1378 CCTGAGG CC^T[ACGT]AGG BalI 860 TGGCCA TGG^CCA BanI 1193 GGTGCC G^G[CT][GA]CC BbsI 571 GAAGAC GAAGAC(2/6) BbvCI 937 GCTGAGG CCTCAGC(-5/-2) BciVI 1392 GGATAC GTATCC(6/5) BclI 134 TGATCA T^GATCA BfaI 1144 CTAG C^TAG BglI 770 GCCGACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 571 GAAGAC GAAGAC(2/6) BsaBI 1066 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1058 GAGACC GGTCTC(1/5) BseRI 1003 GAGGAG GAGGAG(10/8) BspHI 731 TCATGA T^CATGA BstAPI 1257 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 835 CGCG CG^CG Bsu36I 1378 CCTGAGG CC^T[ACGT]AGG CfoI 323 GCGC GCG^C DraIII 1258 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 860 TGGCCA [CT]^GGCC[GA] EarI 918 GAAGAG CTCTTC(1/4) Eco81I 1378 CCTGAGG CC^T[ACGT]AGG EcoRI 274 GAATTC G^AATTC EcoRV 191 GATATC GAT^ATC FauI 991 GCGGG CCCGC(4/6) FbaI 134 TGATCA T^GATCA HgaI 1119 GCGTC GACGC(5/10) HhaI 323 GCGC GCG^C HinP1I 323 GCGC G^CGC HincII 604 GTCAAC GT[CT]^[GA]AC HindII 604 GTCAAC GT[CT]^[GA]AC Ksp632I 918 GAAGAG CTCTTC(1/4) MaeI 1144 CTAG C^TAG MaeII 665 ACGT A^CGT MamI 1066 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 860 TGGCCA TGG^CCA MscI 860 TGGCCA TGG^CCA MseI 227 TTAA T^TAA MvnI 835 CGCG CG^CG PaeR7I 155 CTCGAG C^TCGAG PflMI 612 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 430 CTGCAG CTGCA^G PvuII 935 CAGCTG CAG^CTG RcaI 731 TCATGA T^CATGA RsaI 1162 GTAC GT^AC SacI 19 GAGCTC GAGCT^C SexAI 31 ACCAGGT A^CC[AT]GGT SfcI 430 CTGCAG C^T[GA][CT]AG SmaI 1370 CCCGGG CCC^GGG Tru9I 227 TTAA T^TAA Van91I 612 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 155 CTCGAG C^TCGAG XmaCI 1370 CCCGGG C^CCGGG XmaI 1370 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 274 GAATTC [GA]^AATT[CT] 1045 GAATTT ApoI 274 GAATTC [GA]^AATT[CT] 1045 GAATTT AspHI 19 GAGCTC G[AT]GC[AT]^C 1264 GTGCTC AvaI 155 CTCGAG C^[CT]CG[GA]G 1370 CCCGGG AvaII 905 GGACC G^G[AT]CC 923 GGACC BanII 19 GAGCTC G[GA]GC[CT]^C 93 GGGCTC BglII 44 AGATCT A^GATCT 656 AGATCT BpmI 5 CTCCAG CTGGAG(16/14) 1123 CTGGAG BsgI 1255 CTGCAC GTGCAG(16/14) 694 GTGCAG BsiHKAI 19 GAGCTC G[AT]GC[AT]^C 1264 GTGCTC BsmAI 1058 GAGAC GTCTC(1/5) 3 GTCTC BsoBI 155 CTCGAG C^[CT]CG[GA]G 1370 CCCGGG Cac8I 74 GCAGGC GC[ACGT]^[ACGT]GC 839 GCAAGC DraII 941 AGGCCCT [GA]G^G[ACGT]CC[CT] 971 AGGCCCT EcoO109I 941 AGGCCCT [GA]G^G[ACGT]CC[CT] 971 AGGCCCT EcoT14I 617 CCTTGG C^C[AT][AT]GG 1088 CCAAGG MslI 126 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1275 CACTCCTGTG MspA1I 63 CAGCGG C[AC]G^C[GT]G 935 CAGCTG SmlI 155 CTCGAG C^T[CT][GA]AG 1243 CTCAAG StyI 617 CCTTGG C^C[AT][AT]GG 1088 CCAAGG TfiI 51 GATTC G^A[AT]TC 1303 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI Asp700I BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI